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http://dx.doi.org/10.5352/JLS.2008.18.6.845

Purification and Characterization of β-Lactamase Secreted from Bacillus sp. J105 Strain having β-Lectam Antibiotics Resistance.  

Cho, Kyeong-Soon (Public Health and Environment Institute of Pusan)
Kang, Byoung-Won (BK21 Center for Silver-Bio industrialization Project, Dong-A University)
Seo, Min-Jeong (Department of Biotechnology, Dong-A University)
Lee, Young-Choon (Department of Biotechnology, Dong-A University)
Lee, Jai-Heon (Department of Genetic Engineering, Dong-A University)
Joo, Woo-Hong (Department of Biology, Changwon National University)
Choi, Yung-Hyun (Department of Biochemistry, College of Oriental Medicine, Dong-eui University)
Lim, Hak-Seob (Department Bioinstitute, MILLENNIUM PROMISE CO., LTD.)
Kim, Jeong-In (School of Food and Life Science, Inje University)
Seo, Kwon-Il (Department of Food and Nutrition, Sunchon National University)
Jeong, Yong-Kee (Department of Biotechnology, Dong-A University, Department Bioinstitute, MILLENNIUM PROMISE CO., LTD.)
Publication Information
Journal of Life Science / v.18, no.6, 2008 , pp. 845-851 More about this Journal
Abstract
${\beta}-Lactamase$, secreted from Bacillus sp. J105 strain was purified to a single band on SDS-PAGE by ammonium sulfate precipitation, ion exchange column chromatography and gel-filtration. The molecular weight of the purified enzyme was 31 kDa on SDS-PAGE and its isoelectric point was 7.35. Optimal pH and temperature for enzymatic reaction were 5 and $40^{\circ}C$, respectively. As a result of total amino acid composition analysis of the purified enzyme, Gly and Ala were occupied 14.1 and 13.3 mole %, respectively. Km and Vmax value of purified enzyme were 1.33 mM and 0.36 mM/ml using ampicillin as a substrate, respectively.
Keywords
Antibiotics; Bacillus sp. J105${\beta}-lactamase$${\beta}-lactam$; enzyme purification;
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1 Wu, W., P. S. F. Mezes, Y. Q. Yanag, R. W. Balacher and J. O. Lampen. 1985. Cloning and sequencing of the $\beta$-lactamase I gene of Bacillus cereus 5/B and its expression in Bacillus subtilis. J. Bacteriol. 163, 487-492
2 Cho, K. S., W. D. Kang, D. H. Kim, H. Beak, H. K. Sung and Y. K. Joung. 1998. Characterization of resistant bacterium against $\beta$-lactam antibiotics. J. Dong-eui univ. 28, 403-410
3 Fu, K. P. and H. C. Neu. 1981. The role of inducible $\beta$- lactamases in the antagonisms seen with certain cephalosporin combinations. J. Antimicrob. Chemother. 7, 104-107   DOI
4 Hashizume, T., A. Yamaguchi and T. Sawai. 1986. Outer membrane permeability of imipenem in comparison with other $\beta$-lactam antibiotics. J. Antibiot. 1, 153-156
5 Jan, E. P., B. L. Masecar and M. J. Jervos. 1988. Characterization and comparison of two penicillinase-producing strains of Streptococcus (Enterococcus) faecalis. Antimicrob. Agents and Chemother. 32, 122-124   DOI   ScienceOn
6 Lampe, M. F., J. B. Allan, B. H. Minshew and J. C. Sherris. 1982. Mutational enzymatic resistance of Enterobacter species to beta-lactam antibiotics. Antimicrob. Agents and Chemother. 21, 655-660   DOI   ScienceOn
7 Livermore, D. M., F. Moosdeen, M. A. Lindridge, P. Kho and J. D. Williams. 1986. Behaviour of TEM-1 beta-lactamase as a resistance mechanism to ampicillin, mezlocillin and azlocillin in Escherichia coli. J. Antimicrob. Chemother. 17, 139-146   DOI
8 Minami, S., M. Inoue and S. Mitsuhashi. 1980. Purification and properties of acephalosporinase from Enterobacter cloacae. Antimicrob. Agents and Chemother. 18, 853-857   DOI   ScienceOn
9 Neu, H. C. 1984. Current mechanisms of resistance to anti- microbial agents in micro-organisms causing infection in the patient at risk for infection. Am. J. Med. 76(6A), 11
10 Weber, K. and M. Osborn. 1971. Measurement of molecular weights by electrophoresis on SDS-polyacrylamide gel. Meth. Enzymol. 26, 3-27
11 Danziger, L. H. and S. L. Pendland. 1995. Bacterial resistance to $\beta$-lactam antibiotics. Am. J. Health-Syst. Pharmacy 52, 3-8
12 Kasic, J. E. and L. Peacham. 1968. Properties of $\beta$-lactamase produced by three species of Mycobacteria. Biochem. J. 107, 675 -682   DOI
13 Fisher, J. F., S. O. Meroueh and S. Mobashery. 2005. Bacterial resistance to $\beta$-lactam antibiotics: Compelling opportunism, compelling opportunity. Chem. Rev. 105, 395-424   DOI   ScienceOn
14 Lineweaver, H. and D. Burk. 1934. The Determination of Enzyme Dissociation Constants. J. Am. Chem. Soc. 56, 658-666   DOI
15 Minami, S., A. Yotsuji, M. Inoue and S. Mitsuhashi. 1980. Induction of $\beta$-lactamase by various b-lactam anti-biotics in Enterobacter cloacae. Antimicrob. Agents and Chemother. 18, 382-385   DOI   ScienceOn
16 Lowry, O. H., N. J. Posebrough, L. A. Farr and R. J. Randal. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265-275
17 Massova, I. and S. Mobashery. 1998. Kinship and diversification of bacterial penicillin-binding proteins and beta- lactamases. Antimicrob. Agents Chemother. 42, 1-17   DOI   ScienceOn
18 Sawai, T., I. Takahashi and S. Yamagishi. 1970. Iodometric assay method for beta-lactamase with various beta-lactam antibiotics as substrates. J. Bacteriol. 13, 910-913
19 Poole, K. 2004. Resistance to beta-lactam antibiotics. Cell. Mol. Life Sci. 61, 2200-2223
20 Sawai, T., M. Kano and K. Tsukamoto. 1982. Characterization of eight $\beta$-lactamases of Gram-negative bacteria. J. Bacteriol. 152, 567-571
21 Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685   DOI   ScienceOn
22 Ghuysen, J. M. 1991. Serine-lactamases and penicillinbinding proteins. Annu. Rev. Microbiol. 45, 37-67   DOI   ScienceOn
23 Joris, B. 1988. The active-site-serine penicillin-recognizing enzymes as members of the Streptomyces R6l DD-peptidase family. Biochem. J. 250, 313-324   DOI
24 Mehta, R. J. and C. H. Nash. 1978. $\beta$-Lactamase activity in yeast. J. Antibiot. 31, 239-240   DOI
25 Bush, K., G. A. Jacoby and A. A. Medeiros. 1995. A functional classification scheme for beta-lactamases and its correlation with molecular structure. Antimicrob. Agents Chemother. 39, 1211-1233   DOI   ScienceOn
26 Ogawara, H. 1978. Production and property of $\beta$-lactamase in Streptomyces. Antimicrob. Agents and Chemother. 8, 402-408
27 Kushner, D. J. and C. Broil. 1977. Penicillinase formation by blue green algae. Microbiology 112, 219-223   DOI
28 Livermore, D. M. 1995. beta-Lactamases in laboratory and clinical resistance. Clin. Microbiol. Rev. 8, 557-584
29 Kitzis, M. D., D. Billet-klein, F. W. Goldstein, R. Williamson, G. T. Van Nhieu, J. Carlot and J. F. Acar. 1988. Dissemination of the novel plasmid-mediated $\beta$-lactamase CTX-1, which confers resistance to broad-spectrum cephalosporins, and its inhibition by $\beta$-lactamase inhibitors. Antimicrob. Agents and Chemother. 32, 9-14   DOI   ScienceOn
30 Kelly, J. A., O. Dideberg, P. Charlier, J. P. Wery, M. Libert, P. C. Moews, J. R. Knox, C. Duez, C. Fraipont and B. Joris. 1986. On the origin of bacterial resistance to penicillin: Comparison of a beta-lactamase and a penicillin target. Science 231, 1429-1431   DOI   ScienceOn