• 한국균학회지
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• 제10권3호
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• pp.111-117
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• 1982

The carpophores of Cryptoporus volvatus collected in Gyeong-gi Province of Korea were extracted with water and a protein-polysaccharide fraction was obtained after dialysis and lyophilization. The antitumor activity of this fraction was tested against sarcoma 180 implanted in A-strain mice. The tumor inhibition ratio was 80.4% in case of the high dose group (50mg/kg, ip, 10 days) and 70.3% in the low dose group (20mg/kg, ip, 10 days). The protein­polysaccharide fraction was chemically analyzed and was found to be a complex of a protein which was 18.2% of the fraction when determined by Lowry-Folin method, and a polysaccharide which was 55.3% of ther fraction when determined by Anthrone method. Their subunits were identified as four monosaccharides and 18 amino acids by gas-liquid chromatography and amino acid autoanalysis.

• 한국동물학회지
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• 제30권4호
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• pp.371-378
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• 1987

The fractions of serum proteins, the levels of serum immunoglobulins and the positive rate of HBs Ag were investigated in Korean patients with Down's syndrome. Mean concentrations (g/dl) of serum protein, albumin, a1,-globulin fraction, a2-globulin fraction, $\beta$-globulin fraction and Y-globulin fraction were 7.80$\pm$1.44, 3.89$\pm$0.74, 0.26$\pm$0.09, 0.80$\pm$0. 18, 1.00토0.29 and 1.85$\pm$0.64, respectively. Mean concentrations(mg/dl) of IgG, IgA and IgM were 1,682.17$\pm$600.26, 247.39$\pm$180.86 and 170.87$\pm$79.90, respectively. The positive rate of HBs Ag was 21.74% These results revealed higher levels of Y-globulin fraction, IgG and positive rate of HBs Ag in patients with Down's syndrome than in normal population.

• 셀메드
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• 제4권1호
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• pp.5.1-5.8
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• 2014

Healthcare-associated infection represents a frequent cause of mortality that increases hospital costs. Due to increasing microbial resistance to antibiotics, it is necessary to search for alternative therapies. Consequently, novel alternatives for the control of resistant microorganisms have been studied. Among them, plant antimicrobial protein presents enormous potential, with flowers being a new source of antimicrobial molecules. In this work, the antimicrobial activity of protein-rich fractions from flower tissues from 18 different species was evaluated against several human pathogenic bacteria. The results showed that protein-rich fractions of 12 species were able to control bacterial development. Due its broad inhibition spectrum and high antibacterial activity, the protein-rich fraction of Hibiscus rosa-sinensis was subjected to DEAE-Sepharose chromatography, yielding a retained fraction and a non-retained fraction. The retained fraction inhibits 29.5% of Klebsiella pneumoniae growth, and the non-retained fraction showed 31.5% of growth inhibition against the same bacteria. The protein profile of the chromatography fractions was analyzed by using SDS-PAGE, revealing the presence of two major protein bands in the retained fraction, of 20 and 15 kDa. The results indicate that medicinal plants have the biotechnological potential to increase knowledge about antimicrobial protein structure and action mechanisms, assisting in the rational design of antimicrobial compounds for the development of new antibiotic drugs.

• Archives of Pharmacal Research
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• 제13권4호
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• pp.330-337
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• 1990

The crude polysaccharide from Panax ginseng prepared by hot water extration and precipiation with ethanol was further fractionated into neutral and acidic fractions by DEAE- cellulose ion exchange chromatography. The chemical compositions were 85.0% carbohydrorate and 15.0% protein for the neutral fraction, and 28.4% carbohydrate, 10.0% protein and 29.0% uronic acid for the acidic fraction. The acidic fraction was more effective in increasing of the ratio of spleen to body weight, the number of antibody secreting cells to SRBC and phagocytic activity of reticuloendothelial system, as well as antitumor activity against the solid form of sarcoma 180 in ICR mice than the neutral fraction. All polysaccharide fractions were mitogenic to cultured spleen cells of C57BL/6 mice. However, FA was different from FN in the co-mitogenicities with lectin mitogens. Both crude and acidic fractions potentiated remarkably the mitogenic activity of PHA-P or LPS in dose-dependent manner but neutral fraction enhanced only that of LPS. Three polysaccharide fractions had no effect on that of Con A. These results suggest that the acidic fraction may stimulate B and Td cells as well as macrophages while the neutral fraction may simulate only B cells and macropages.

• 농업과학연구
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• 제44권3호
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• pp.392-399
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• 2017

The present study was conducted to investigate the effects of different dietary proteins as fraction-enriched protein, defined by Cornell net carbohydrates and protein system (CNCPS), on in vivo ruminal fermentation pattern and blood metabolites in Holstein steers fed total mixed ration (TMR) containing 17.2% crude protein. Four ruminally cannulated Holstein steers in a $4{\times}4$ Latin square design consumed TMR only (control) and TMR with rapeseed meal (AB1), soybean meal (B2), and perilla meal (B3C). Each protein was substituted for 23.0% of crude protein in TMR. Rumen digesta were taken through ruminal cannula at 1 h interval during the feeding cycle in order to analyze ruminal pH, ammonia-N, and volatile fatty acids (VFA). Plasma metabolites in blood taken via the jugular vein after the rumen digesta sampling were analyzed. Feeding perilla meal significantly (p < 0.05) decreased mean ruminal pH compared with control and the other protein feeding groups. Compared with control, feeding protein significantly (p < 0.05) increased ruminal ammonia-N concentration except for AB1. Statistically (p > 0.05) similar total VFA appeared among control and the supplemented groups. However, control, AB1, and B2 showed higher (p < 0.05) acetate concentrations than B3C, and propionate was vice versa. CNCPS fractionated protein significantly (p < 0.05) affected concentrations of albumin and total protein in blood; i.e. plasma albumin was lower for control and B2 groups than AB1 and B3C groups. Despite lack of significances (p > 0.05) in creatinine and blood urea nitrogen, AB1 and B2 groups were numerically higher than the others.

• KSBB Journal
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• 제7권4호
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• pp.302-307
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• 1992

protein phosphatase 2A는 bovine brain homogenate의 세포질 fraction에서 얻어졌다. 기질로서 인산화된 histione H1을 이용하여 측정한 phosphatase 의 활성은 dipalmitoyIphophatidylcholine(DPPC) 혹은 phosphatidylserine/DPPC의 혼합물로 구성된 liposome의 존재하에서 저해되었다. Protein phosphatase 2A의 lipid membrane에의 결합은 다중층 지질막의 혼합물 계에서 liposome 의 양이 증가함에 따라서 상등액 중의 phosphatase의 활성이 감소하는 것으로 확인할 수 있었다. 또한 [$^{125}I$]protein phosphatase 2A가 liposome과 동시에 용출되는 것으로도 확인되었다. 그러나 liposome에 대한 protein phosphatase의 친화력은 높지 않았다. 한편, okadaic acid와 liposome은 협동으로 phosphatase의 활성을 감소시켰다. 이것은 okadaic acid가 lipid membrane이나 membrane에 결함한 phosphatase에는 결합하지 않는다는 것을 의미한다. 그러므로 lipid membrane에 의한 protein phosphatase 2A의 활성 저해 효과는 phosphatase 2A와 lipid membrane과의 결합에 의한 것이라고 설명될 수있다.

• Journal of Plant Biology
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• 제36권2호
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• pp.171-176
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• 1993

Protein kinase C, a protein related in PI cascade, was partially purified from the cytosol protein of etiolated plants of Glycine max by DEAE-52 cellulose chromatography and phenylsepharose chromatography. When the DEAE column was eluted with 0-0.8 M linear gradient KCl, tow fractions were found that increased the phosphorylation of histon H1 about five and nine-fold in the presence of 5 $\mu\textrm{g}$/mL phosphatidylserine and 0.5 $\mu\textrm{g}$/mL diolein, respectively. These fractions were used as DEAE pool. The reaction eluted with relatively high concentration of KCl was loaded on phyenylsepharose column with 5 mM CaCl2 and eluted with 1 mM EGTA. A fraction contained the protein kinase C, which increased the phosphorylation of the histon H1 was fractionated. To determine the molecular weight of PKC, the fraction eluted from phenylsepharose column was analyzed by 5~15% polyacrylamide gel electrophoresis after concentrated with the Amicon membrane (YM10). That revealed two bands corresponding to 60 and 65 kGy by silver staining of the gel, respectively.

• 한국균학회지
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• 제8권2호
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• pp.115-116
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• 1980

To investigate mechanism of antineoplastic activities of the protein-polysaccharide fraction of Coriolus versicolor (Fr.) Quel., the mycelium of the fungus was grown in a liquid medium and was extracted with hot water. The extract was purified and used for examining its effects upon immune response in mice. The fraction was administered at a dose of 20mg/kg/day to ICR mice for five days. After ten days the mice were immunized by sheep red blood cells. The plaque-forming cells (PFC) in their spleen were increased in the treated mice. The number of PFC in case of the protein-removed polysaccharide was lower than that of the entire protein-­polysaccharide fraction of the fungus.

• Parasites, Hosts and Diseases
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• 제30권3호
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• pp.209-218
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• 1992

혈청이나 뇌척수액에서 특이항체를 증명하여 유구낭미충증을 진단함에 있어 유구낭미충의 낭액이 가장 민감하고도 특이한 항원임은 이미 알려졌다. 낭액을 항원으로 사용하여도 다른 기생충증과 빈도는 낮지만 교차반응이 발생하므로 교차반응을 일으키는 구성단백질에 대하여 더 관찰할 필요가 있다. 이 연구에서는 유구낭미충 낭액을 Sephacryl S-300 Superfine 젤로 분획하고, 낭액 및 그 분획이 낭액을 면역시켜 만든 토끼 항혈청 (RACF)과 반응하는 양상을 면역전기영동법으로 관찰하여 낭액의 구성 단백질의 성질을 분석하고자 하였다. 유구낭미충 장액을 젤여과법으로 분획하면 제 3분회과 제 4분회에 단백질이 가장 많이 포함되었다. 제 3분획은 disc-PAGE상 분자량 150 kDa인 것으로 이미 알려진 Band C가 주(주) 구성 단백질이었다. 제 4분획은 분자량이 약 65kDa일 것으로 추정되는 Band N 단백질이 주 구성단백질이었다. 낭액에 대한 토끼 항혈청을 낭액과 면역전기영동법으로 반응시키면 주 침강대 2개와 작은 침강대 7∼8개를 형성하였는데 낭액의 제 3분획은 주 침강대 중 밖에 위치한 긴 침강대를 형성한 반면, 제4분획은 주 침강대 중 안쪽으로 위치한 짧은 침강대를 형성하였다. 유구낭미충과 혈청학적 교차반응을 가장 많이 일으키는 포충(포충, hydatid cyst)의 낭액과 RACF(유구낭미충 낭액에 대한 토끼 항혈청)을 면역전기영동법으로 반응시킨 바, 제 4분획이 반응한 위치에서 침강대를 형성하여 제 4분획의 주구성 단백질이 포충증과의 혈청학적 교차반응의 원인인 것으로 추정하였다. 유구낭미충증으로 이미 진단한 환자의 혈청 중 효소면역 측정 법으로 항체가가 매우 높았던 혈청 8개를 선택하여 유구낭미충 낭액 및 유구낭미충 충체 추출액과 각각 면역전기영동법으로 반응시킨 바 각각 7명에서 침강대를 형성하였고, 제 3분회 및 제 4분회으로 면역전기영동법으로 반응시킨 환자혈청 4개 중에서는 제 3분획에서는 4개 모두가 침강대를 형성한 반면 제 4분 회에서는 침강대가 혈청 2개에서 형성되었다.

• 약학회지
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• 제32권5호
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• pp.313-318
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• 1988

Radioprotective ginseng protein fraction was isolated from Korean white ginseng and its cytotoxic effect on CHO-K1 cells was studied by the method of measuring the relative cell survival and total cellular protein content (FRAME method). When ginseng protein at the dose of 300, 600, 900, $1200{\mu}g/ml$ was treated to cells for 24 hrs, the relative survival was significantly decreased at the concentration of above $600{\mu}g/ml$, indicating the presence of cytotoxic effect of the protein at certain concentration. When cellular protein content was measured after ginseng protein at the dose of 300, 600, 900, $1200\;{\mu}g/ml$ was treated, the amount of cellular protein was significantly reduced at the concentration above $600{\mu}g/ml$ in the case of 24 hr treatment and at all concentrations including $300{\mu}g/ml$ in the case of 72 hr treatment. The data suggest that the protein may inhibit cell growth, resulting in the reduction of live cells in culture. $ID_{50}$ value which is the concentration of ginseng protein that reduces the total cellular protein content to 50% of the control was calculated as 2276.86 and $1323.32\;{\mu}g/ml$ in groups treated for 24 and 72 hr, respectively. Since $ID_{50}$ value of above $1000{\mu}g/ml$ indicates very weak cytotoxicity, the ginseng protein seems to exert very weak cytotoxic effect on CHO-K1 cells.