• Microbiology and Biotechnology Letters
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• v.16 no.4
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• pp.259-264
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• 1988

Two forms of extracellular inulinase, designated as PI and PII were detected in the crude enzyme preparation from n species of Pseudomonas isolated from soil. PI and PII were purified to homogeneity by ammonium sulfate fractionation, DEAE Sephadex A-50 chromatography, Sephadex G-100 and Sephadex G-200 gel filteration. Both isoenzymes catalyzed specifically and endowise the cleavage of the $\beta$-2,1-fructofranoside linkage of inulin, and displayed no action upon sucrose, raffinose and levan. The optimal pH values for the PI and PII enzyme were pH 5.5 and 6.0, respectively and the highest activity of the two enzymes was observed at 55$^{\circ}C$. The Km values of PI and PII were calculated to be 2$\times$10$^{-3}$M and 5$\times$10$^{-3}$M, respectively.

• Tunnel and Underground Space
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• v.29 no.6
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• pp.508-522
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• 2019

Because of the slope failure, not only property damage but also human damage can occur, slope stability analysis should be conducted to predict and reinforce of the slope. This paper, defines the ground areas that can be characterized in terms of slope failure such as Rockmass jointset, Rockmass fault, Soil, Leakage water and Crush zone in sloped images. As a result, it was shown that the deep learning instance segmentation network can be used to recognize and automatically segment the precise shape of the ground region with different characteristics shown in the image. It showed the possibility of supporting the slope mapping work and automatically calculating the ground characteristics information of slopes necessary for decision making such as slope reinforcement.

• Journal of the Korean Society of Groundwater Environment
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• v.5 no.2
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• pp.101-109
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• 1998

Hydraulic property of fissured aquifers often depends on geologic structure which acts main channel of groundwater flow. We treated theories of linear flow related to vertical geologic structure. Then, we analyzed the result of two pumping tests conducted in Okmyeong-ri area (Kyeongbook province) using fractal model and found hydraulic characteristic of the fissured aquifer in this area. According to the pump test analyses, groundwater flow around the holes (pumping well D9; observation wells C3 and D7) of test 1 is linear. and is controlled by vertical geologic structure with infinite length and infinitesimally small width. On the other hand, around the hole D10 (pumping well) of test 2, groundwater flow is pseudo-radial (n=1.9) or radial (n=2). Thus, the characteristic of fractured aquifer often shows variable groundwater flow spatially and temporally.

• Journal of Sericultural and Entomological Science
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• v.40 no.2
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• pp.126-130
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• 1998

A noninsecticidal strain, Bacillus thuringiensis NTB-88, isolated from Korean soil, had a typical bipyramidal parasporal inclusion and its serotype is identical to B. thuringiensis subspmorrisoni (H8a8b). To elucidate differences between insecticidal and noninsecticidal strains, we compared strain NTB-88 to other toxic B. thuringiensis subsp. morrisoni strains (HD-12 and PG-14). Restriction endonucleases digested plasmid DNA patterns showed that strain NTB-88 was different from lepidopteran-toxic strain, HD-12, but it was similar to dipteran-toxic strain, PG-14. The gene type of strain NTB-88 was different from those of other insecticidal strains, Furthermore, the NH2-terminal amino acid sequence of crystal protein of strain NTB-88 had no relation to those of the previously known $\delta$-endotoxins in other toxic strains as well as HD-12 and PG-14 strains. Therefore, the noninsecticidal crystal protein in strain NTB-88 is novel and its property is different from insecticidal ones.

• Textile Coloration and Finishing
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• v.21 no.1
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• pp.38-45
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• 2009

First of all, the properties imparted to PET fabrics are resistance to and recovery from creasing or wrinkling when wet or dry; high resistance to stretch in the filament yarns but not in the staple; high abrasion resistance; good texture and appearance; resistance to heat ageing; good chemical resistance and good resistance, behind glass, to sunlight. But, the low moisture regain of PET fabric conduces to static troubles in textile processing. Furthermore, garments made from PET may, during wear, develop electric charges which attract to the fabric particles of soil(dirt, swarf, dust) flying in the air, so that the cuffs of shirts, for example, become soiled quickly and are not easily laundered clean. The sericin constitutes 25$\sim$30％ of silk protein and surrounds the fibroin fiber with sticky layer that supports the formation of a cocoon. The useful biochemical properties of sericin protein are oxidation resistant, antibacterial, UV resistant, hydrophilic property, and good affinity with hydrophobic material. These properties can be used as an improving reagent or a coating agent for natural and synthetic fibers, fabrics, and other intermediate products. The sericin is also applied to cross-link, and can be blended with other materials. In this study, we modified the surface of PET fabric by mixture of sericin finishing agent; sericin, polyuretane binder and 1,2,3,4-butanetetracarboxylic acid (BTCA) cross-link agent. Also, we investigated the finshing effect; moisture regain, stiffness, handle, drape and electrostatic. The moisture regain of PET fabric treated with sericin finishing agent was higher than that of untreated PET fabric. As a result of evaluating influence about handle of PET fabrics treated with sericin finishing agent, it was confirmed that the sericin finishing agent could be use as a linen like finishing agent.

• Archives of Pharmacal Research
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• v.27 no.5
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• pp.570-575
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• 2004

2-Hydroxymuconic semialdehyde (2-HMS) dehydrogenase catalyzes the conversion of 2-HMS to 4-oxalocrotonate, which is a step in the meta cleavage pathway of aromatic hydrocarbons in bacteria. A tomC gene that encodes 2-HMS dehydrogenase of Burkholderia cepacia G4, a soil bacterium that can grow on toluene, cresol, phenol, or benzene, was overexpressed into E. coli HB 101, and its gene product was characterized in this study. 2-HMS dehydrogenase from B. cepacia G4 has a high catalytic efficiency in terms of V$_{max}$K$_{max}$ towards 2-hydroxy-5-methyl-muconic semialdehyde followed by 2-HMS but has a very low efficiency for 5-chloro-2-hydroxymuconic semialdehyde. However, the enzyme did not utilize 2-hydroxy-6-oxo-hepta 2,4-dienoic acid and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid as substrates. The molecular weight of 2-HMS dehydrogenase from B. cepacia G4 was predicted to be 52 kDa containing 485 amino acid residues from the nucleotide sequence of the tomC gene, and it exhibited the highest identity of 78% with the amino acid sequence of 2-HMS dehydrogenase that is encoded in the aphC gene of Comamonas testosteroni TA441. 2-HMS dehydrogenase from B. cepacia G4 showed a significant phylogenetic relationship not only with other 2-HMS dehydrogenases, but also with different dehydrogenases from evolutionarily distant organisms.sms.

• Korean Journal of Human Ecology
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• v.7 no.2
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• pp.81-91
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• 1998

The subjects in this research were 368 girls in high school for survey, and wearing sensation and physiological responses were investigated through wearing trials on human body in climatic chamber based on these results from the survey. The results are as follows : 1. They enjoyed wearing t-shirts, jackets, vests, and blouses in order for the upper clothes, and they preferred t-shirts to blouses. For the lower clothes, they enjoyed slacks much more than skirts. The weight of clothes was significantly heavier in the group where they wore the uniforms(U-group) than in the group where they wore the free styles(F-group). When they chose the school wear, activity was the most important of all, and the maintenance was the least. 2. As the classes were a little cool and dry, most of them dissatisfied the environment. The degree of the satisfaction of the class environment and properties to it were higher in the U-group than in F-group. 3. In the textiles, colors, styles, activity, static electricity, seasonal property, and easiness of putting on and taking off the clothes, F-group was more satisfied than U-group. U-group was more satisfied than F-group in the soil of the clothes. 4. The thermal comfort, thickness, and tightness of the clothes were not significantly different between the groups. The clothes of U-group was heavier than those of F-group, and the tactile sensation in U-group was worse than F-group. In U-group the students felt the skirts very inconvenient when they acted. 5. The weight of the clothes influenced the wearing sensation, therefore the heavier the clothes were the less satisfied they felt. 6. The inside temperature of clothes was significantly higher in U-group than in F-group. The skin temperatures of abdomen and arm were significantly higher in U-group than in F-group, while the skin temperatures of thighs and legs were significantly lower in U-group than in F-group. U-group felt heavier than F-group in wearing the clothes. Therefore the improvement of the clothes weight is needed.

• Journal of Korean Society of Environmental Engineers
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• v.28 no.7
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• pp.764-769
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• 2006

In the electrokinetic(EK) process, oxygen production by electrolysis was proportional to current density. The dissolved oxygen (DO) concentration in anode tank and bioreactor increased with the circulation rate of electrolyte. The bacterial population in bioreactor rapidly increased by the supplement of current, but the DO concentration deceased by the increased bacterial oxygen consumption. From the results of EK bioremediation for pentadecane-contaminated soil, the bacterial population and removal efficiency at 1.88 $mA/cm^2$ were lower than those at 0.63 $mA/cm^2$. This is because the high oxygen production rate largely increased the production rate of organic acids, which reduced the electrolyte pH and bacterial activity. At 0.63 $mA/cm^2$, the highest bacterial population and removal efficiency could be obtained due to the appropriate oxygen production and small decrease in pH.

• Journal of the Korean GEO-environmental Society
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• v.17 no.6
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• pp.5-12
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• 2016

This paper deals with the result of thermal resistance test with backfill materials as bottom ash by using backfill material. Bottom ash, one of coal ashes, can be reused to replace sand because of its similar engineering properties. But without considering the thermal property, the abuse of bottom ash resulted in damage for existing structures. To investigate the thermal conductivity of bottom ash, laboratory tests for thermal resistance of that were carried out in this study. Thermal properties of bottom ash was compared with those of in-situ soil, sand, backfill material which can be applied as filling material. The tests were classified by water contents defined as the major influence factor. The beneficial use method of bottom ash was suggested as backfilling material.

• Proceedings of the Korean Geotechical Society Conference
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• 2009.09a
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• pp.905-912
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• 2009

1-g shaking table test is conducted to evaluate the dynamic behavior of a soil-structure system under seismic loading condition. A consistent similitude law between the model and prototype is needed to predict the behavior of the prototype structure, quantitatively. The natural frequency of geomaterial decreases with the increase of shaking intensity because of the non-linear property of the geomaterial. This phenomenon affects the applicability of similitude laws in 1-g shaking table tests. In this study, a simple method is suggested to determine the frequency of the input motions in 1-g tests in order to enhance the applicability of similitude laws. Modified input frequency is calculated using the frequency ratio with consideration of the variation of the natural frequency according to the intensity of input ground acceleration. To verify the applicability of the suggested method, a series of 1-g shaking table tests were performed for three different sizes of model piles having an overburden mass on their heads by varying the acceleration and the frequency of input motion. The acceleration amplification ratio on the overburden mass, the lateral displacement at the pile head and the maximum bending moment along the pile depth were measured. The projected behaviors of the virtual prototype based on the measured values of the model tests, where the input frequencies were calculated by the new method, showed good consistency, verifying the applicability of the suggested method.