• Proceedings of the Korean Society of Developmental Biology Conference
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• 2009.02a
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• pp.49-57
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• 2009

The cloning of canids was succeeded in 2005, several years after the birth of Dolly the sheep and also after the cloning of numerous other laboratory and farm animal species. The delay of successful somatic cell nuclear transfer (SCNT)was due to the unique reproductive characteristics of the female dogin comparison to other domestic mammals, such as ovulation of immature canine oocyte and a requirement of 25 days for the completion of meiosis within the oviduct (Holst & Phemister, 1971). When the technology for the recovery of in vivo matured oocyte was established, the application of cloning also became possible and cloned dog offspring were obtained. This report summarizes the progress of technical procedures that are required for cloning canids and the application of this technique. The first cloned dog, Snuppy, was achieved using an in vivo-matured oocyte which was enucleated and transferred with an adult skin cell of male Afghan hound. After establishment of a criterion of well-matured oocyte for the improvement of SCNT efficiency, we obtained three cloned female Afghan hound and a toy poodle cloned from 14 year-old aged Poodle using SCNT through this factor. To date, cloned dogs appeared to be normal and those that have reached puberty have been confirmed to be fertile. Through application of canine SCNT technique, first, we demonstrated that SNCT is useful for conserving the breed of endangered animal from extinction through cloning of endangered gray wolves using inter-species SCNT and keeping the pure pedigree through the cloning of Sapsaree, a Korean natural monument. Secondly, we showed possibility of human disease model cloned dog and transgenic cloned dog production through cloning of red fluorescent protein expressing dog. Finally, SCNT can be used for the propagation of valuable genotypes for making elite seed stock and pet dog. In summary, dog cloning is a reproducible technique that offers the opportunity to preserve valuable genetics and a potential step towards the production of gene targeted transgenic cloned dogs for the study of human diseases.

• Journal of Korean Society of Forest Science
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• v.96 no.2
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• pp.170-177
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• 2007

The objectives of this study were to select early, late, and abundant flowering trees of black locust from domesticated and introduced cultivars, and prediction of flowering period by calculation of accumulated temperature in spring. Four cultivars (Debreceni-2, Pusztavacs, Jaszkiseri, and Rozsaszin AC) from Hungary and a cultivar from Beijing, China, were introduced, propagated by seed and planted in a seed orchard. For domesticated black locust, 63 cultivars from 10 locations throughout the country were selected and propagated by root cutting. Criteria for selection of domesticated cultivars were abundant flowering, long flowering period, or abundant nectar production with, if possible, straight stems. Accumulated temperature was calculated from data of a nearby weather station by accumulating daily maximum temperature minus 5 degree Celsius from January 1 up to the date reaching 880 degrees. Daily mean temperature was also used to calculate accumulated temperature up to the date reaching 450 degrees. The percentages of two-year and three-year-old flowering trees propagated by root cutting were higher than that of trees propagated by seeds, while four-year-old trees all flowered regardless of propagation methods. Among the domesticated cultivars, all the cultivars from Ganghwa showed abundant flowering with highest nectar production of 6.5 ul per flower, which was 100% more than other domesticated cultivars and 50% more than Debreceni-2 cultivar with highest nectar production among the introduced cultivars from Hungary. At the end of the eight years of observations, two trees of Debreceni-2 cultivars and a tree from Beijing, China were selected for early flowering trees which flowered 2 to 3 days earlier than average trees, while a tree of Debeceni-2 and three trees from Bejing were selected for late flowering trees which flowered 2 to 3 days later than average trees. It is possible to extend the flowering period of black locust by 4 to 6 days by planting early and late flowering cultivars together. Abundant flowering trees were unable to be selected due to severe damages by leaf gall midges which killed many trees and reduced the crown size of the remaining trees in the seed orchard, and which were first found in Korea in 2001 and now damaging most of the black locust forests in Korea. The prediction of flowering period by accumulated temperature indicated that black locust flowered to a peak when accumulated daily maximum temperature reached 880 degrees Celsius, and when daily mean temperature reached 450 degrees.

• FLOWER RESEARCH JOURNAL
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• v.18 no.3
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• pp.179-185
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• 2010

This study was carried out to investigate the optimal culture conditions for gametophytes growth and sporophytes regeneration of Pyrrosia linearifolia in order to provide for the masspropagation system foundation of Pyrrosia linearifolia using their life cycle. Among many different media, 2MS medium was most effective in prothallus proliferation. Prothallus growth was promoted as the total concentration of nitrogen sources increased, and the best result was observed on 120 mM nitrogen. The best concentration of sucrose was 3%. The addition of 5~20 mM IAA, NAA, BA and kinetin promoted the propagation of prothallus. But 2iP demonstrated the most inhibitory effect on prothallus proliferation. Gametophytes shaking-cultured with liquid medium showed similar growth with solid medium and normal formation of reproductive organs. Shoot regeneration was most effective on 1/8MS medium, but growth was promoted on 1/2MS medium. For promotion of shoot regeneration and growth, the suitable concentrations of sucrose and $NaH_2PO_4$ were 1% and $50{\sim}100mg{\cdot}mL^{-1}$ in 1/8MS medium, respectively.

• FLOWER RESEARCH JOURNAL
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• v.19 no.3
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• pp.164-167
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• 2011

A new spray rose cultivar 'Glory Purple' was bred from the cross between a pink spray cultivar 'Macarena' and a pink spray cultivar 'Million Pink' at the National Institute of Horticulture & Herbal Science. The cross was made in 2004 and a candidate genotype which named as 'Glory Purple' was selected in 2009 after three years investigation for agronomical characters from 2007 to 2009. 'Glory Purple', a purple colored spray cultivar, has vigorous growth and has powdery mildew resistance. The major characteristics of this cultivar are high yield and long stem with relatively large flower size with $157stems/m^2/year$ in yield, 63.5 cm in mean length of cut flower, 6.8 cm in mean flower diameter, 25.78 in mean petal number, and 10.2 days in mean vase life. This cultivar is suitable for propagation by both cutting and grafting methods. The consumer's preference of this cultivar is relatively higher than that of the control cultivars, 'Pinky and Charming'.

• Korean journal of applied entomology
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• v.60 no.4
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• pp.423-429
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• 2021

The ovipositional characteristics of the endangered species Prosopocoilus astacoides blanchardi was investigated for indoor mass-rearing. This insect required 3 weeks or more for mating after emergence when introduced at the 1:1 ratio of male to female. Among temperatures studied at 16, 20, 24, 28, and 32℃, the pre-ovipositional period of P. astacoides blanchardi was longest at 16℃ with an average of 109 days after emergence, while it was shortest at 32℃ with an average of 59.4 days. The number of eggs laid at 24℃ was highest with an average of 40.6 per female. The average lifespan of adults at 16℃ was 208.8 days for males and 263.9 days for females, while at 32℃ 96.1 days for males and 130.3 days for females. The number of laid eggs was dependent on insect rearing chamber size. The oviposition-trees did not significantly (P > 0.05) affect the number of laid eggs and ovipositional period. The results of this study could be used as rearing data for the restoration and mass propagation of the endangered species P. astacoides blanchardi.

• Journal of the Korean Wood Science and Technology
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• v.47 no.4
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• pp.519-532
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• 2019

The objectives of this study were to evaluate the effects of shelling ratio and resin content on the properties of bamboo oriented strand board (BOSB) from betung (Dendrocalamus asper) and to determine the correlation between the results of dynamic and static bending tests. Strands were steam-treated at $126^{\circ}C$ for 1 h under 0.14 MPa pressure and followed by washing with 1% NaOH solution. Three-layer BOSB with the core layer perpendicular to the surface was formed with shelling ratios (face:core ratio) of 30:70; 40:60; 50:50; 60:40 and binded with 7% and 8% of phenol formaldehyde (PF) resin with the addition of 1% of wax. The evaluation of physical and mechanical properties of BOSB was conducted in accordance with the JIS A 5908:2003 standard and the results were compared with CSA 0437.0 standard for commercial OSB (Grade O-1). Non-destructive testing was conducted using Metriguard Model 239A Stress Wave Timer which has a wave propagation time from 1 to $9,999{\mu}s$ and a resolution of $1{\mu}s$. BOSB with 8% resin content showed better physical and mechanical properties than those with 7% resin content. The increase of the face layer ratio improved the strength of BOSB in parallel direction to the grain. The results suggested that shelling ratio of 50:50 could be used as a simple way to reduce PF resin requirements from 8% to 7% and to meet the requirements of CSA 0437.0 standard. The results of non-destructive and destructive tests showed a strong correlation, suggesting that non-destructive test can be used to estimate the bending properties of BOSB.

• The Journal of the Convergence on Culture Technology
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• v.7 no.4
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• pp.721-726
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• 2021

Plant cells have a totipotencial capacity, the ability of each cell to produce a new complete individual through development. By applying this, several technologies are being developed for widespread application of somatic embryogenesis by processing hormones in vitro as a method of propagation of plants. In order to use this technology, in Kalanchoe pinnata, a plant capable of asexual reproduction with more regular cell division, kinetin belonging to cytokinin and picloram among hormones belonging to auxin were added in combination and treated for 8 weeks, and then the typical performance was evaluated. As a result of our experiment, the rooting effect in leaf slices showed a 70% incidence rate at a picloram concentration of 0.1 mg/L. It has been proven that a concentration difference of 1:5-1:10 in the ratio of kinetin and picloram is effective. It is the experimental result that the effect of auxin is essential for the development of Kalanchoe roots. As for the effect of shooting, the incidence rate was 60% at the picloram concentration of 0.5 mg/L. The kinetin concentration from 0.5 and 1.0 mg/L and has a significant effect on development. It has been proven that the ratio of kinetin to picloram is effective with a concentration difference of 1:1-1:2. These results show that the combination of cytokinin and auxin is crucially important for shooting. It is thought that it can be the basis of a technology for inducing mass proliferation in vitro by inducing direct organogenesis with a combination of hormones.

• Journal of Powder Materials
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• v.29 no.1
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• pp.47-55
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• 2022

Tungsten carbide is widely used in carbide tools. However, its production process generates a significant number of end-of-life products and by-products. Therefore, it is necessary to develop efficient recycling methods and investigate the remanufacturing of tungsten carbide using recycled materials. Herein, we have recovered 99.9% of the tungsten in cemented carbide hard scrap as tungsten oxide via an alkali leaching process. Subsequently, using the recovered tungsten oxide as a starting material, tungsten carbide has been produced by employing a self-propagating high-temperature synthesis (SHS) method. SHS is advantageous as it reduces the reaction time and is energy-efficient. Tungsten carbide with a carbon content of 6.18 wt % and a particle size of 116 nm has been successfully synthesized by optimizing the SHS process parameters, pulverization, and mixing. In this study, a series of processes for the high-efficiency recycling and quality improvement of tungsten-based materials have been developed.

• Journal of the Daesoon Academy of Sciences
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• v.44
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• pp.263-297
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• 2023

This study aims to understand human nature in Daesoon Thought. In addition, it seeks to determine the characteristics of human nature and how the theory of human nature in Daesoon Thought relates to the direction of the Basic Works and the Three Major Societal Works of Daesoon Jinrihoe. In order to understand the theory of human nature in Daesoon Thought, I have classified various theories of human nature that appear in the Confucian tradition and have analyzed and reviewed them. I used this framework of types as a methodological criterion to study the theory of human nature found in Daesoon Thought. In Daesoon Thought, human nature is defined clearly as the conscience that is honest and truthful. Also, it accepts humans' basic biological needs, the pursuit of clothing, food, and sexual desires as part of human nature. This view can be evaluated as quite similar, although not perfectly consistent, with the theory of human nature posited by Wang Fuzhi (王夫之), who defined human nature as a combination of morality and biological desires. The theory of human nature in Daesoon Thought emphasizes human morality, but at the same time, it fully accepts that biological needs are basic elements of human life. In addition, it shows a characteristic that strongly implies a religious tendency as it recognizes that human nature is internalized through divine commands issued by the Supreme God of the Ninth Heaven (九天上帝 Gucheon Sangje), the transcendent and absolute ruler. The Basic Works of Daesoon Jinrihoe, which are Propagation, Edification, and Cultivation, aim to restore human nature to its innate state of honesty and truthfulness. Among the Three Major Societal Works, we can see that the works of Social Welfare and Charity Aid are implemented in accordance with the accepting acknowledgment of basic human needs (the pursuit of clothing, food, and sexual desires), and helping people meet their needs in appropriate ways and to appropriate degrees.

• Journal of the Daesoon Academy of Sciences
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• v.22
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• pp.471-507
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• 2014

Edification in Daesoonjinrihoe is not only a phenomenon that occurs following the differences of religious experience or spiritual development among the community members, which enables the members to share teaching and learning experiences with one another, but also an issue determined as one of the major activities of the religious order and a plan for achieving the purpose of the religious order-Podeokchenha(Wordly Propagation), Gujechansaeng (Salvation of all mankind) and Jisangcheonguk Geonseol(Building of earthly paradise). The purpose of this article is to clarify its concept and provide an example of edification, through considering the historical model for edification to help the cultivators with their work of edification. The archetype of edification of Daesoonjinrihoe was formed and gradually developed in phases by Sangje, Kang Jeungsan, the Supreme God(姜甑山, 1871-1909), Doju, Jo Jeongsan(趙鼎山, 1895-1958) and Dojeon, Park Wudang(朴牛堂, 1917-1995), by the three of whom the Religious Authority was succeeded. Sangje descended to the human world and preached to people to live by the rule of Haewon Sangsaeng(Resolution of grievances for the mutual beneficences of all life) and set an example of abolishing the old customs, living in mutual beneficences and having respect for human being. Doju, in revering the last will of Sangje, established the religious order by setting its creed, rituals and activities, which formed most contents of the archetype of edification. Dojeon set up a religious faith system by firmly establishing the Religious Authority and performed the True Law in accordance with Sangje's program of heaven to educate the cultivators to achieve the goal of self-cultivation following the last will of Doju. Through this, a perfect method to reach the state of Dotong(The Truly Unified State of Dao) is fulfilled. In this way, the archetype of edification was formed in the process of succession of Religious Authority. In conclusion, edification in Daesoonjinrihoe contributes to a 'systematic conveyance and understanding' through the historical archetype of edification, and it can be described as a concept that becomes a model to put into practice the 'True Law' of teachings given by two Sangjes for Dotong. Therefore, edification of Daesoonjinrihoe is drawing attention of its development as an important activity that realizes the ultimate value of the religious order because it solves the problems of immorality(absence of Dao), disorder and disregard of human value generated from the other side of this material civilization, with the truth of Haewon Sangsaeng, and has a function of rebuilding and leading the individuals and the society to the Truly Unified State of Dao through performing of the True Law.