• 미생물학회지
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• 제33권2호
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• pp.105-110
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• 1997

한국에서 분리한 U. maydis에서 바이러스 혹은 dsRNA를 가지고 있는 A series와 SH series를 조합하여 옥수수에서 인위적으로 mating시켜 A45, A23, A211, A310, SH614, SH24의 6개 교배형 균주를 분리하였다. 새로운 교배형 균주에서 바이러스 dsRNA를 비교 분석하여 본 결과, mating전 양친형 균주에서 보이고 있는 H, M, L strand의 dsRNA 양상을 모두 가지고 있고 균주간 특별한 molecular exclusion 현상은 보이지 않았다. 교배형 6개으 균주에서 바이러스를 분리하였다. 분리된 바이러스로부터 dsRNA를 분석한 결과 균주간 mating시 dsRNA 재조합을 통한 새로운 encapsidation은 발생하지 않는다는 것을 확인하였다. Toxin test 결과, SH614는 SH9, SH10, SH11 균주에 대해서, A310과 SH24의 2종은 SH11 균주에 대해 특이적 성장억제 현상을 보였다. 이는 mating에 따른 dsRNA의 재조합이 toxin gene의 발현에 영향을 미치지 않는다는 것을 시사한다.

• The Plant Pathology Journal
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• 제36권6호
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• pp.558-569
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• 2020

Fusarium head blight (FHB) is a devastating fungal disease of wheat (Triticum aestivum L.). The lack of genetic resources with stable FHB resistance combined with a reliable and rapid screening method to evaluate FHB resistance is a major limitation to the development of FHB resistant wheat germplasm. The present study utilized an immature wheat spike culture method to screen wheat spike culture derived variants (SCDV) for FHB resistance. Mycotoxin concentrations determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS) correlated significantly (P < 0.01) with FHB severity and disease progression during in vitro spike culture. Selected SCDV lines assessed for FHB resistance in a Fusarium field disease nursery in Carman, Manitoba, Canada in 2016 showed significant (P < 0.01) correlation of disease severity to the in vitro spike culture screening method. Selected resistant SCDV lines were also crossed with an elite cv. CDC Hughes and the progeny of F₂ and BC₁F₂ were screened by high resolution melt curve (HRM) analyses for the wheat UDP-glucosyl transferase gene (TaUGT-3B) single nucleotide polymorphism to identify resistant (T-allele) and susceptible (G-allele) markers. The progeny from the crosses were also screened for FHB severity using the immature spike culture method and identified resistant progeny grouped according to the HRM genotyping data. The results demonstrate a reliable approach using the immature spike culture to screen for FHB resistance in progeny of crosses in early stage of breeding programs.

• Asian-Australasian Journal of Animal Sciences
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• 제12권1호
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• pp.1-4
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• 1999

Beijing White Chickens laying larger eggs and smaller eggs were respectively used as parental individuals for mating to produce the F1 progeny and then the F1 progeny individuals mated to produce 125 individuals of the F2 progeny. Three bands associated with the egg weight performance were identified from DNA fingerprints of the 125 individuals generated with a bovine minisatellite probe BM6.5B. The simple linear correlation analysis showed that the coefficients of correlation between frequencies of the three bands (DB1, DB2 and DB3) and egg weights were -0.6, -0.6 and 0.9, respectively.

• Asian-Australasian Journal of Animal Sciences
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• 제14권11호
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• pp.1505-1510
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• 2001

A method for mapping quantitative trait loci (QTL) was introduced incorporating the information of mixed progeny from complex pedigrees. The method consisted of two steps based on single marker analysis. The first step was to examine the marker-trait association with a mixed model considering common environmental effect and reversed QTL-marker linkage phase. The second step was to estimate QTL effects by a weighted least square analysis. A simulation study indicated that the method incorporating mixed progeny from multiple generations improved the accuracy of QTL detection. The influence of within-genotype variance and recombination rate on QTL analysis was further examined. Detecting a QTL with a large within-genotype variance was more difficult than with a small within-genotype variance. Most of the significant marker-QTL association was detectable when the recombination rate was less than 15%.

• International Journal of Industrial Entomology and Biomaterials
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• 제13권2호
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• pp.113-117
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• 2006

Heterosis was studied involving two multivoltine silkworm breeds viz, APM1 and SLKSPM through rearing and isozyme analysis. A positive significant heterotic effect was observed in fecundity, hatching % and survivability. The heterobeltiosis was observed only in fecundity and hatching %. Isozyme analysis of ${\alpha}-esterase$ showed variation in loci and allelic expression. The allele with heterozygosity $(Est-2^{12})$ was observed at the Est-2 locus in $F_1$ progeny. Est-3 was observed in $F_1$ progeny, whereas it was completely absent in both parental lines. The present study suggests that the markers ($Est-2^{12}$ and Est-3) targeted for introgression may be useful for the improvement of fecundity and survivability as the phenomenon of heterosis was observed only in $F_1$ progeny.

• Journal of Microbiology
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• 제34권2호
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• pp.166-171
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• 1996

Genetic analysis was conducted on newly isolated coliphages form soil by using a RAPD assay. From the initial result, the coliphages were turned out to be different form one another but were closely related to .psi..lambda. due to the fact that they shared the samed RAPD maker in which other T phage testings failed to show. By using the primers EC01 or EC02, a fast genetic mutation of .psi.C1 was found by producing specific RAPD markers on the phages from the first filial progeny to the second filial progeny. When we made a RAPD assay with combined primers (EC01, EC05 and EC08), the genetic mutation was again confirmed in .psi.C1. The assay detection showed mutations in other coliphages such as .psi.C2 and .psi.C3 by revealing specific RAPD bands among different progeny phages, where genetic instability of the coliphages in implied.

• Journal of Plant Biotechnology
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• 제31권2호
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• pp.103-108
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• 2004

본 연구는 나리의 종간잡종 육성체계를 확립하기 위한 기초자료를 얻고자 Oriental과 Asiatic 잡종 (OA-hybrid) 의 후대 획득에 필요한 실험을 수행하여 얻은 결과이다. 체세포 배수화를 통한 이질 4배체 OA-hybrid 3계통으로 화분발아 실험을 수행한 결과, 0-80% 범위로 계통간 차이가 현저하였다. 이질 4배체 OA-hybrid를 자방친 또는 화분친으로 사용하고 Asiatic 또는 Oriental hybrid와 교배시 후대 획득수에 차이가 있었는데 4배체 OA-hybrid를 화분친으로 이용하였을 때, 그리고 상대친을 Asiatic hybrid로 교배하였을 때 후대획득이 현저히 용이하였다. 그러나, 이질 4배체 F$_1$ OA-hybrid를 자방친으로 사용하거나 Oriental hybrid와 교배하면 후대 획득률이 현저히 저하되거나 또는 전혀 후대생산이 이루어지지 않았다. 이질 4배체 F$_1$ OA-hybrid와 교배시 상대친의 ploidy level은 4배체 보다는 2배체가 유리하였다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2015년도 추계학술대회 및 정기총회
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• pp.37-37
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• 2015

dsRNA was found in malformed cultures of Lentinula edodes strain FMRI0339, one of the three most popular sawdust cultivated commercial strains of shiitake, and was also found in healthy-looking fruiting bodies and actively growing mycelia. Cloning of the partial genome of the dsRNA revealed the presence of the RdRp sequence of a novel L. edodes mycovirus (LeV), and sequence comparison of the cloned amplicon showed an identical sequence to known RdRp genes of LeV found in strain HKA. The meiotic stability of dsRNA was examined by measuring the ratio of the presence of dsRNA among sexual monokaryotic progeny. More than 40% of the monokaryotic progeny still contained the dsRNA, indicating the persistence of dsRNA during sexual reproduction. Comparing the mycelia growth of monokaryotic progeny suggested that, although variations in the growth rate existed among progeny and virus infection was observed in highly actively growing progeny, there appeared to be a tendency toward a lower frequency of virus incidence in actively growing progeny. This study attempted to cure the edible mushroom L. edodes strain FMRI0339 of the L. edodes mycovirus (LeV) in order to obtain an isogenic virus-free fungal strain as well as a virus-infected strain for comparison. Mycelial fragmentation, followed by being spread on a plate with serial dilutions resulted in a virus-free colony. Viral absence was confirmed with gel electrophoresis after dsRNA-specific virus purification, Northern blot analysis, and PCR using reverse transcriptase (RT-PCR). Once cured, all of fungal cultures remained virus-free over the next two years. Interestingly, the viral titer of LeV varied depending on the culture condition. The titer from the plate culture showed at least a 20-fold higher concentration than that grown in the liquid culture. However, the reduced virus titer in the liquid culture was recovered by transferring the mycelia to a plate containing the same medium. In addition, oxygen-depleted culture conditions resulted in a significant decrease of viral concentration, but not to the extent seen in the submerged liquid culture. Although no $discernable phenotypic changes in colony morphology were observed, virus-cured strains showed significantly higher growth rates and mycelial mass than virus-infected strains. We were also explored effects of LeV on fruiting body formation and mushroom yield. The fruiting body formation yield of virus-free L. edodes was larger than virus-infected L. edodes. These results indicate that LeV infection has a deleterious effect on mycelial growth and fruiting body formation. In addition, we have been investigated host-parasite interaction between L. edodes and its mycovirus interaction to study viral mechanism by establishment of proteomics.

• Journal of the Korean Data and Information Science Society
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• 제15권1호
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• pp.75-81
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• 2004

We selected, in previous research, a major DNA Marker 235bp of ILSTS035 microsatellite locus in progeny test Hanwoo chromosome 6. We apply a major DNA Marker 235bp to perormance valuation Hanwoo chomosome 6. We use bootstrap BCa method and calculate confidence interval. A major DNA Marker 235bp is verified that it does not have environmental effect but affects primely economic trait factor.

• 농업과학연구
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• 제2권2호
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• pp.341-356
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• 1975

본 연구는 육성후대의 3개 동기생육형 합성품종과 서부 oregon의 저온다습한 기후 하에 선발된 7개인자형의 다계교배 파생계통들의 능력을 검정하기 위하여 대비품종으로 다수성품종 Fawn을 공시하여 대전의 충남대학교 공과대학 실험포장에서 수행하였다. 특히 광합성과 관련이 깊은 식물체 및 잎의 주요형질과 아울러 재식후 첫여름의 조사로 생산량을 조사 분석하였던바 주요한 결론을 요약하면 다음과 같다. 1) 파생계통 및 합성품종군과 대비품종 상호간에는 생엽중의 유전적인 차이를 나타내었던바 다계교배 파생계통군의 평균 생엽중이 가장 무거웠고 다음이 합성품종군 이었으며 대비품종 Fawn이 가장 가벼웠다. 한편 다계교배 파생계통군 대에서는 인자형들간에 건엽중의 유전적 차이를 나타내고 있었다. 2) 엽면적 역시 파생계통 군합성품종군 및 대비품종간에 유전적 차이를 보였는데 동기생육형 합성품종군의 평균 엽면적이 대비품종 Fawn에 비해 현저히 넓었으며 다계교배 파생계통군이 가장 넓은 평균 엽면적을 보았다. 3) 합성품종군 파생계통군 및 대비품종간에 특정엽중(단위엽면적당엽중)의 차이는 인정되지 않았는데 앞으로 이특저엽중의 중간변이에 관한 연구가 요망된다. 4) 엽폭은 파생계통군, 합성품종군 및 대비품종간에 현저한 유전적인 차이가 인정되었던바 다계교배 파생계통군의 평균엽폭이 가장 넓었고 다음에 동기새육형 합성품종군 이었으며 대비품종 Fawn이 가장 좁았다. 5) 제1회 예취전 초장은 대비품종 Fawn이 동계생육형 합성품종이나 파생계통에 대해 현저히 길었으며 한편7개인자형의 다계교배 파생계통간에서는 초장의 편차가 크게 나타났으나 초장의 재생력은 품종 파생계통간 혹은 내에서 유의차가 인정되지 않았다. 6) 일차 예취전의 주폭(株幅) 즉 포장피복능력은 동기생육형 합성품종에서 컷고 한편 1차예취후의 주폭은 다계교배파생계통들간에 상당한 차이를 나타내었던바 유전적인 차이가 충분히 인정되었다. 8) 제1차예취시의 청예수량은 합성품종의 후기세대에서 상당한 개선을 보았으며 다계교배 파생계통의 7개 인형들간에는 상당한 유전적인 차이를 보였다. 다계교배 파생계통군의 평균수량은 대비 품종이나 동기생육형 합성품종의 수량보다 현저히 높았던바 이로서 보다 수량성이 높은 품종의 개량이 앞으로 가능할 것이라고 추측된다. 9) 여름동안의 청예수량 및 초장 재생력으로 보면 동기생육형 품종군 및 파생계통들간의 재생력에는 대체로 차이가 없었다. 10) 전체적으로 볼때 특정엽중 및 제1회 예취전초장을 제외한 전형질에서 모두 유의성은 인정되는 것은 아니라 하더라도 다계교배 파생계통군이 가장 우수하였고 다음이 합성품종군이었으며 대비품종 Fawn이 가장 떨어졌다. 그리고 다계교배 파생계통의 7개 인자형 간에는 유전적인 차이가 인정되었다. 11) 통계적인 유의성이 모두 인정되는것은 아니나 모두 형질에서 품종 1002가 1001 및 1000두품종보다 우수 하였다. 12) 여러형질간의 상관연구의 결과는 잘일치되고 있는바 보다나은 품종의 개량을 위한 바람직한 인자형의 선발에 기여 할수 있을 것이다.