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Preliminary Studies on the Role of ${\alpha}-Esterase$ Isozymes in Quantitative Traits of Two Multivoltine Silkworm (Bombyx mori L.) Races and their $F_1$ Hybrid  

Kumaresan P. (Central Sericultural Germplasm Resources Centre)
Somasundaram P. (Central Sericultural Germplasm Resources Centre)
Kumar K. Ashok (Central Sericultural Germplasm Resources Centre)
Urs S. Raje (Central Sericultural Germplasm Resources Centre)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.13, no.2, 2006 , pp. 113-117 More about this Journal
Abstract
Heterosis was studied involving two multivoltine silkworm breeds viz, APM1 and SLKSPM through rearing and isozyme analysis. A positive significant heterotic effect was observed in fecundity, hatching % and survivability. The heterobeltiosis was observed only in fecundity and hatching %. Isozyme analysis of ${\alpha}-esterase$ showed variation in loci and allelic expression. The allele with heterozygosity $(Est-2^{12})$ was observed at the Est-2 locus in $F_1$ progeny. Est-3 was observed in $F_1$ progeny, whereas it was completely absent in both parental lines. The present study suggests that the markers ($Est-2^{12}$ and Est-3) targeted for introgression may be useful for the improvement of fecundity and survivability as the phenomenon of heterosis was observed only in $F_1$ progeny.
Keywords
Heterosis; Isozyme; Quantitative traits; Multivoltine; Silkworm races;
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1 Das, S. K., S. Pattnaik, B. Ghosh, T. Singh, B. P. Nair, S. K. Sen and G Subba Rao (1994) Heterosis analysis in some three-way crosses of Bombyx mori L. Sericologia 34, 51-61
2 Eguchi, M., N. Yoshitake and H. Kai (1965) Types and inherit­ance of the blood esterase in the silkworm, Bombyx mori L. Jpn. J. Genet. 40, 15-19   DOI
3 Falconer, D. S. and T. F. C. Mackay (1996) Changes of gene frequency. Introduction to Quantitative Genetics. 4th edition. A.w. Longman Ltd., Harlow England, pp. 23-77
4 Kumaresan, P., R. K. Sinha and K. Thangavelu (2003) Hetero­sis studies in some elite multivoltine silkworm (Bombyx mori L.) races with popular bivoltine $NB_{4}D_{2}$. Int. J. Indust. Entomol. 7, 221-229
5 Rai, B. (1979) Heterosis breeding. Agri-biological publication, Delhi, India
6 Selander, R. K. (1976) Genetic variation in natural popula­tions; in Molecular Evolution Ayala FJ. (ed). Sinauer Associ­ates, Inc., Suderland, Massachusetts, pp. 277
7 Yoshitake, N. and M. Akiyama (1965) Genetic aspect on the esterase activities of the egg in the silkworm, Bombyx mori L. (in Japanese). Jpn. Sericult. Sci. 34, 327-332
8 Bhargava, S. K., V. Thiagarajan and R. K. Datta (1993) Hybrid vigour in the silkworm, Bombyx mori L. G. it. Ent. 6, 449­-453
9 Richardson, B. J., P. R. Baverstock and M. Adams (1986) Allozyme Electrophoresis. A Hand book for Animal Sys­tematic and Population studies, Atademic Press New York
10 Suzuki, M. G., T. Terada, M. Kobayashi and T. Shimada (1999) Diapause-associated transcription of BmEts, a gene encod­ing an ETS transcription factor homology in Bombyx mori. Insect Biochem. Mol. Biol. 29, 339-347   DOI   ScienceOn
11 Harada, C. (1961) Heterosis of the quantitative characters in silkworm. Bul. Seric. Expt. Stat. Jpn. 17, 50-52
12 Vinnani, S. S. (1994) Heterosis and hybrid rice breeding. Monographs on Theoretical and Applied Genetics., Springer-Verlag. 22, 1-38
13 Rao, P. R. T., B. Ghosh, S. M. Moorthy, S. K. Das, G. C. Roy, A. K. Sengupta and S. K. Sen (1998) Combining ability, gene action and heterosis through introgressive hybridization in Bombyx mori L.; in Perspectives in Cytology and Genet­ics. Manna, G. K. and S. C. Roy (eds.), 9, 461-471. AICCG Publication Kalyani University, India
14 Hayashi, Yoshio and Yun Fun-Ja (2002). Geographical studies on chemato pscyche infascia (Trichoptera : Hydropschinae) by Esterase isozymes. Biogeography 4, 1-6
15 Eguchi, M. and Yoshitake (1967) Interrelation of non-specific esterase among various tissues in the silkworm Bombyx mori L. Jpn. J. Sericult. 36, 193-198
16 Krishnaswamy, S. (1978) New technology of silkworm rear­ing. Bulletin No.2, CSR & T1, Mysore, India, pp. 1-18
17 Weeden, N. F. and J. L. Wendel (I989) Genetics of plant isozymes; in Isozymes in plant biology, Soltis, D. E. and P. S. Soltis (eds), Dioscorides Press, Portland, Oregon, USA, pp. 46-72
18 Yoshitake, N. and M. Eguchi (1965) Distribution of the blood esterase types in various strains of the silkwonn, Bombyx mori L. (in Japanese). Jpn. Sericult. Sci. 34, 95-98
19 Bernardo, R. (1992) Relationship between single cross perfor­mance and molecular marker heterozygosity. Theor. Appl. Genet. 83, 628-634
20 Banuprakash, K. G., R. Govindan and M. C. Devaiah (1994) Heterosis observed in the hybrids between some improved multivoltines and bivoltines of Bombyx mori L. Insect Sci. Applic.15, 313-321
21 Laemmli, E. K. (1970) Cleavage of structure proteins during assembly of the head of bacteriophage T4. Nature 227, 680­-685   DOI   ScienceOn