• Food Science of Animal Resources
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• v.28 no.3
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• pp.276-282
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• 2008

It is estimated that over 80% of deer antlers produced in the world are consumed in Korea. However, mislabeling or fraudulent replacement of costly antlers with cheaper ones is one of the most common problems in the domestic antler market. Therefore, there is a great need for the development of technology to identify species of antlers. This study was carried out to develop an accurate and reliable method for the identification and authentication of species or subspecies of antlers using DNA sequence analysis and comparison of mitochondrial cytochrome band D-loop region genes among antlers of five deer species, Cervus elaphus sibericus, Cervus elaphus canadensis, Cervus nippon, Cervus elaphus bactrianus and Rangifer tarandus. A variable region of cytochrome band D-loop genes was amplified using PCR with specifically designed primers and sequenced directly. The cytochrome band D-loop region genes showed different DNA sequences between the species of antlers and thus it is possible to differentiate between species on the basis of sequence variation. To distinguish between reindeer (Rangifer tarandus) antlers and other deer antlers, PCR amplicons of the cytochrome b gene were digested with the restriction enzymes NlaIV and TaqI, respectively, which generates a species-specific DNA profile of the reindeer. In addition, samples of 32 sliced antlers labeled Cervus elaphus sibericus from commercial markets were collected randomly and the mt DNA D-loop region of these antler samples was sequenced. Among the antler samples investigated, only 62.5% were from Cervus elaphus sibericus, and others were from Cervus elaphus bactrianus (25.0%), elk (Cervus elaphus canadensis) and reindeer (Rangifer tarandus). Our results suggest that DNA sequencing of mt DNA and PCR-RFLP methods using NlaIV and TaqI enzymes are useful for the identification and discrimination of deer antler species by routine analysis.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.10 no.1
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• pp.64-75
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• 1999

Objective：The purpose of this study was to investigate the prevalence and characteristics of comorbid disorders and associated symptoms in pervasive developmental disorder(PDD) and to examine the correlation between associated symptoms and developmental characteristics in PDD children. Method：The sample consisted of 209 cases of PDD and 143 cases of developmental language disorder(DLD)(control group) who were treated at the Seoul National Mental Hospital from Jan. 1996 to Mar. 1999. The diagnostic work based on DSM-IV criteria was performed by one or two child psychiatrists, while the clinical feature was evaluated by doctors’s notes, occupational/speech therapy reports, and results of social maturity scale(SMS), childhood autism rating scale(CARS), and psycho-educational profile(PEP). Two groups were compared on a wide range of measures including comorbid disorders, associated symptoms, treatment drugs, and PEP. The relation between associated symptom & PEP was investigated in total(106 cases) and in each dignostic group. Sixty-four cases of PDD were divided into three groups by CARS and then compared on associated symptoms. Result：The prevalence of comorbid disorder was 19.6% in PDD, 41.2% in DLD. The rate of manifestation of 13 associated symptoms was 31.47% in PDD, 22.13% in DLD on the average. Associated symptoms significantly high in PDD were preoccupation, obsession, self-mutilation, stereotypy, sleep problems, and odd response. In total patient group, associated symptoms that significantly influenced PEP were preoccupation, self-stimulation, stereotypy, inappropriate affect, sleep problems, and odd response. But, in each diagnostic group, no associated symptom influenced PEP. Associated symptoms significantly different between the 3 groups of CARS were stereotypy, anxiety, and sleep problems. Conclusion：These preliminary results suggest that developmental characteristics may influence associated symptoms in PDD children and a realistic approach considering minute diagnosis by associated symptoms and comorbid disorders is required.

• The Journal of Korean Academy of Sensory Integration
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• v.7 no.1
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• pp.27-36
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• 2009

Introduction : Occupational therapists commonly use clinical observation to assess neuromuscular function witch is a fundamental component of sensory integration function. Clinical Observation of Motor and Postural Skills (COMPS) is a standardized assessment with seven items and used to screen if a child's problem is due to neuromuscular and sensory integration system. However, developmental standard of the test need to be validated with Korean children. Objective : This study is purposed to propose developmental standard of the COMPS for Korean children. Method : Seven to nine years old students (76 male and 70 female) participated in this study. In order to find out any difference by gender and age, the data was analyzed using t-test and ANOVA. Results : There is no significant difference by gender for all other items except Prone Extension Position (PEP). There is significant difference between children who are 7 years old and those who are 9 years old for Slow Motion(SM), Finger-Nose Touching (FNT), Asymmetrical Tonic Neck Reflex (ATNR), Supine Flexion(SF). There is also significant difference between those who are 8 years old and 9 years old for SM, FNT, ATNR. However, there is no significant difference between those who are 7 years and 8 years old. Conclusions : This study examines any difference in neuromuscular characteristics by age among school-aged children, based on the COMPS. The result of this study will provide a good evidence to establish developmental standard of COMPS for Korean children. It issuggested to continue further standardization work of the COMPS in order to establish a developmental standard for Korean children.

• The Journal of Engineering Geology
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• v.23 no.3
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• pp.201-216
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• 2013

Ground penetrating radar (GPR) surveys were conducted in a sand tank model in a laboratory and at an alluvial field site to detect the groundwater table and to investigate the influence of saturation on GPR response in the unsaturated zone. In the sand tank model, the groundwater table and saturation in the sand layer were altered by injecting water, which was then drained by a valve inserted into the bottom of the tank. GPR vertical reflection profile (VRP) data were obtained in the sand tank model for rising and lowering of the groundwater table to estimate the groundwater table and saturation. Results of the lab-scale model provide information on the sensitivity of GPR signals to changes in the water content and in the groundwater table. GPR wave velocities in the vadose zone are controlled mainly by variations in water content (increased travel time is interpreted as an increase in saturation). At the field site, VRP data were collected to a depth of 220 m to estimate the groundwater table at an alluvial site near the Nakdong river at Iryong-ri, Haman-gun, South Korea. Results of the field survey indicate that under saturated conditions, the first reflector of the GPR is indicative of the capillary fringe and not the actual groundwater table. To measure the groundwater table more accurately, we performed a GPR survey using the common mid-point (CMP) method in the vicinity of well-3, and sunk a well to check the groundwater table. The resultant CMP data revealed reflective events from the capillary fringe and groundwater table showing hyperbolic patterns. The normal moveout correction was applied to evaluate the velocity of the GPR, which improved the accuracy of saturation and groundwater table information at depth. The GPR results show that the saturation information, including the groundwater table, is useful in assessing the hydrogeologic properties of the vadose zone in the field.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.2 no.1
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• pp.46-58
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• 1999

Purpose: The aim of the present study was to evaluate the long-term clinical profile including the underlying etioligy and the prognostic factors of the neonatal cholestasis. Method: We studied the 190 infants presented with neonatal cholestasis for the last 12 years (from 1981 to 1992). The underlying causes, clinical findings and long-term outcomes were evaluated. And the prognostic factors were also analyzed. Result: Underlying disease were neonatal hepatitis in 101 (idiopathic in 77 and infectious in 24), intrahepatic bile duct paucity in 5, biliary atresia in 79, choledochal cyst in 5. Metabolic disease was not observed in this study. The important clinical problems during follow-up were persistent high fever, gastrointestinal bleeding, hepatic encephalopathy and ascites. The main causes of the death were hepatic encephalopathy and gastrointestinal bleeding. While three fourth of infants with idiopathic and infectious neonatal hepatitis recovered usually within a year, five-year survival rate for biliary atresia was just 40%, the mortality observed usually within the first year after Kasai operation and prognostic factor was the time of operation. Underlying disease was the most important prognostic factor of neonatal cholestasis. Conclusion: This study showed that most common causes of neonatal cholestasis were biliary atresia and idiopathic neonatal hepatitis, infectious neonatal hepatitis, choledochal cyst and Alagille syndrome, but few neonatal cholestasis of genetic or metabolic liver disease was observed. The most important long-term prognostic factor of neonatal cholestasis was the underlying disease.

• Journal of Mushroom
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• v.9 no.2
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• pp.59-62
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• 2011

To develop a new cultivar of King oyster mushroom(Pleurotus eryngii), G09-21 as parental strain was selected by the method of Di-mon crossing between monokaryotic strains derived from ASI 2824(Keunneutari No.2) and dikaryotic strain ASI 2887(Aeryni 3). The Pe21-51($G09-21-10{\times}2844-11$) was shown the best cultural characteristics, selected to be a new cultivar and named as 'Song-A'. The 'Song-A' was formed incompatibility line distinctly in the confrontation growth of parental strains Keunneutari No.2, Aeryni 3 and ASI 2844. The optimum temperature for mycelial growth, fruiting body development and pH arrange were $25{\sim}30^{\circ}C$, $14{\sim}16^{\circ}C$ and pH5~8, respectively. Fruiting body production per bottle was about $94.7{\pm}29.5$ g which is almost 106% quantity compared to that of other cultuvar Keunneutari No.2. And also the stip is thick and long but the number of available stipe is few. Analysis of the genetic characteristics of the new cultivar 'Song-A' showed a different DNA profile as that of the control strains, Keunneutari No.2, Aeryni 3 and ASI 2844, when RAPD(Random Amplified Polymorphic DNA) primers URP4 and 7 were used. This new cultivar 'Song-A' of Pleurotus eryngii is characterized by a small number of primordia formation and the stip is thick and long. Therefore, we expect that this new strain will save of labor and cost by without culling work.

• Journal of the Korean association of regional geographers
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• v.2 no.1
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• pp.115-131
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• 1996

Tourism is multi-faceted phenomenon which involves movement to stay in destinations outside the normal place of residence. Thus, tourism is a composite phenomenon which incorporates the diversity of variables and relationships to be found in the tourist travel process. Tourism-behaviors are of major economic and social significance. With the growth of mass-tourism, the government departments of tourism is to provide many tourist facilities, to develop travel destination and tourism resources. At same time, the growth of mass-tourism has prompted perceptive travellers to raise many questions concerning the many travel destination and the tourism resources of encouraging further tourism demand. Most of tourism have a mental in their minds about various objects. This allowed him to choose tourism destination as a important rules. In tourism geography, behavioral approach to wildness, tourism resources and places has risen since 1960's. The tourist profile can be viewed under two major categories: the tourist social-economic and behavioral characteristic. Particulary, the motivations, attitude, need, values and preference of travel destinations are of crucial importance in contributing to their desion-making process. In this view, this paper is emprical study investigated travel patterns and behavioral characteristic of tourists and potential tourist in East Chonnam. The purpose of this paper is to show the spatial preference and tourism behavior of travel destinations. For this study, we are undertaken the questionary method employed by recreation geographers in order to collect research data for the East Chonnam-citizens functioned as a major tourist demand. The East Chonnam Province have many tourist-places including national park, provincial park, and local tourist-places. Thus, citizen of the East Chonnam evaluate many tourist-places very high, but evaluate the facilities of tourist place very low. The high ranks of preferences to travel destinations among tourist-places are Hyangilam, Geomoon island, Odongis land, Songkwang temple. The major travel destinations occurred the temporary travel trip are Odong island, Sunam temple, Songkwang temple. Heungkook temple, Hyangilam which are the type of mountainous in located inland. The relationship between the degree of preference and tourism behavior of travel trip for the travel destinations does not correlate each other. The result of analysis about the degree of seasonal preferences presented spatial differences according to characteristics of tourist-places. The typical travel destination are divided into seasons : Odong island. Mt. Backwoon, Goemoon island of spring, Goemoon island, Sungbul valley, Banggukpo beach of summer, Songkwang temple, Mt. Pal young of fall. The future of tourism will be rapidly grow with increase of personal mobility and leisure time, chang of tourism behavior. Thus, it is imperative that planning and development for tour-root, facilities of tourist-places should be implemented to increase tourism demand.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.14 no.4
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• pp.201-211
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• 1981

For the effective utilization of sardine, sardinops melanosticta, one of the major coastal fish in Korea, of which annual catch has been increasing from year to year since 1970, it was processed in form of fermented fish paste. The fish were treated with BHA and Teaox-Ⅱ in concentration of $0.01\%\;and\;0.02\%$ to prevent the oxidation of lipid during fermentation and then salted with $20\%$ table salt and fermented at room temperature of $25\pm3^{\circ}C$. The duration of fermentation necessary for the final product with an acceptable taste was determined by sensory evaluation by means of profile method. From the result of sensory evaluation, one month was found to be suitable as the reasonable duration of fermentation. Both BHA and Tenox-Ⅱ in conceatration of $0.02\%$showed a good preventing effect on the lipid oxidation during fermentation. In case of fermented sardine treated with both antioxidants, lipid oxidation occurred little up to two months, whereas the control showed a remarkable deterioration during one month of fermentation. Most of the nucleotides in sardine was decomposed from adenosing triphosphate to inosine and hypoxanthine during the fermentation of one month. The great portion of free amino acids in the extractives of product was occupied by leucine, glutamic acid, isoleucine, alanine, valine and ysine in turn, and their coatent was $59.4\%$ of the total free amino acids. The amount of essential amino acids was $59.4\%$ of the total free amino acids. The contents of 5'-IMP, betaine, trimethylamine oxide and total creatinine in the extractives of product were $1.9{\mu}mole/g,\;4.9mg\%,\;1.0mg\%\;and\;475mg\%$, respectively. According to the omission test, the main constituents of the characteristic taste of fermented sardine could be assumed as free amino acids and a little amount of 5'-IMP.

• Geophysics and Geophysical Exploration
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• v.12 no.1
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• pp.132-142
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• 2009

The Tamar rift valley runs through the City of Launceston, Tasmania. Damage has occurred to city buildings due to earthquake activity in Bass Strait. The presence of the ancient valley, the Tamar valley, in-filled with soft sediments that vary rapidly in thickness from 0 to 250mover a few hundreds metres, is thought to induce a 2D resonance pattern, amplifying the surface motions over the valley and in Launceston. Spatially averaged coherency (SPAC), frequency-wavenumber (FK) and horizontal to vertical spectrum ratio (HVSR) microtremor survey methods are combined to identify and characterise site effects over the Tamar valley. Passive seismic array measurements acquired at seven selected sites were analysed with SPAC to estimate shear wave velocity (slowness) depth profiles. SPAC was then combined with HVSR to improve the resolution of these profiles in the sediments to an approximate depth of 125 m. Results show that sediments thicknesses vary significantly throughout Launceston. The top layer is composed of as much as 20m of very soft Quaternary alluvial sediments with a velocity from 50 m/s to 125 m/s. Shear-wave velocities in the deeper Tertiary sediment fill of the Tamar valley, with thicknesses from 0 to 250m vary from 400 m/s to 750 m/s. Results obtained using SPAC are presented at two selected sites (GUN and KPK) that agree well with dispersion curves interpreted with FK analysis. FK interpretation is, however, limited to a narrower range of frequencies than SPAC and seems to overestimate the shear wave velocity at lower frequencies. Observed HVSR are also compared with the results obtained by SPAC, assuming a layered earth model, and provide additional constraints on the shear wave slowness profiles at these sites. The combined SPAC and HVSR analysis confirms the hypothesis of a layered geology at the GUN site and indicates the presence of a 2D resonance pattern across the Tamar valley at the KPK site.

• Journal of Radiation Protection and Research
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• v.40 no.2
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• pp.79-86
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• 2015

$^3He$ gas has been used for neutron monitors as the neutron converter owing to its advantages such as high sensitivity, good ${\gamma}$-discrimination capability, and long-term stability. However, $^3He$ is becoming more difficult to obtain in last few years due to a global shortage of $^3He$ gas. Accordingly, the cost of a neutron monitor using $^3He$ gas as a neutron converter is becoming more expensive. Demand on a neutron monitor using an alternative neutron conversion material is widely increased. $^{10}B$ has many advantages among various $^3He$ alternative materials, as a neutron converter. In order to develop a neutron converter using $^{10}B$ (actually $B_4C$), we calculated the optimal thickness of a neutron converter with a Monte Carlo simulation using MCNP6. In addition, a neutron converter was fabricated by the Ar sputtering method and the neutron signal detection efficiencies were measured with respect to various thicknesses of fabricated a neutron converter. Also, we developed a 2-dimensional multi-wire proportional chamber (MWPC) for neutron beam profile monitoring using the fabricated a neutron converter, and performed experiments for neutron response of the neutron monitor at the 30 MW research reactor HANARO at the Korea Atomic Energy Research Institute. The 2-dimensional MWPC with boron ($B_4C$) neutron converter was proved to be useful for neutron beam monitoring, and can be applied to other types of neutron imaging.