• 대한한의학회지
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• 제44권2호
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• pp.106-118
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• 2023

Objectives: Network pharmacology is a method of constructing and analyzing a drug-compound-target network to predict potential efficacy and mechanisms related to drug targets. In that large-scale analysis can be performed in a short time, it is considered a suitable tool to explore the function and role of herbal medicine. Thus, we investigated the potential functions and pathways of Chongmyunggongjin-dan (CMGJD) on Alzheimer's disease (AD) via network pharmacology analysis. Methods: Using public databases and PubChem database, compounds of CMGJD and their target genes were collected. The putative target genes of CMGJD and known target genes of AD were compared and found the correlation. Then, the network was constructed using Cytoscape 3.9.1. and functional enrichment analysis was conducted based on the Gene Ontology (GO) Biological process and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathways to predict the mechanisms. Results: The result showed that total 104 compounds and 1157 related genes were gathered from CMGJD. The network consisted of 1157nodes and 10034 edges. 859 genes were interacted with AD gene set, suggesting that the effects of CMGJD are closely related to AD. Target genes of CMGJD are considerably associated with various pathways including 'Positive regulation of chemokine production', 'Cellular response to toxic substance', 'Arachidonic acid metabolic process', 'PI3K-Akt signaling pathway', 'Metabolic pathways', 'IL-17 signaling pathway' and 'Neuroactive ligand-receptor interaction'. Conclusion: Through a network pharmacological method, CMGJD was predicted to have high relevance with AD by regulating inflammation. This study could be used as a basis for effects of CMGJD on AD.

• Journal of Veterinary Science
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• 제23권1호
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• pp.2.1-2.18
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• 2022

Background: Co-infections of the porcine reproductive and respiratory syndrome virus (PRRSV) and the Haemophilus parasuis (HPS) are severe in Chinese pigs, but the immune response genes against co-infected with 2 pathogens in the lungs have not been reported. Objectives: To understand the effect of PRRSV and/or HPS infection on the genes expression associated with lung immune function. Methods: The expression of the immune-related genes was analyzed using RNA-sequencing and bioinformatics. Differentially expressed genes (DEGs) were detected and identified by quantitative real-time polymerase chain reaction (qRT-PCR), immunohistochemistry (IHC) and western blotting assays. Results: All experimental pigs showed clinical symptoms and lung lesions. RNA-seq analysis showed that 922 DEGs in co-challenged pigs were more than in the HPS group (709 DEGs) and the PRRSV group (676 DEGs). Eleven DEGs validated by qRT-PCR were consistent with the RNA sequencing results. Eleven common Kyoto Encyclopedia of Genes and Genomes pathways related to infection and immune were found in single-infected and co-challenged pigs, including autophagy, cytokine-cytokine receptor interaction, and antigen processing and presentation, involving different DEGs. A model of immune response to infection with PRRSV and HPS was predicted among the DEGs in the co-challenged pigs. Dual oxidase 1 (DUOX1) and interleukin-21 (IL21) were detected by IHC and western blot and showed significant differences between the co-challenged pigs and the controls. Conclusions: These findings elucidated the transcriptome changes in the lungs after PRRSV and/or HPS infections, providing ideas for further study to inhibit ROS production and promote pulmonary fibrosis caused by co-challenging with PRRSV and HPS.

• 융합정보논문지
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• 제12권3호
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• pp.85-107
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• 2022

본 연구는 수상 운송업이 전체 산업들과의 관계 속에서 경제 전반에 미치는 유발 효과 및 네트워크 연결성과 네트워크 시각화 분석을 시행하는 것이다. 이를 위하여 산업연관분석과 단위구조행렬을 활용하여 수상 운송업의 각종 유발계수를 분석하며, 단위구조행렬과 역행렬함수를 작성하고 Ucinet 6을 이용하여 네트워크 연결성과 NetDraw를 활용하는 네트워크 시각화 분석을 시행한다. 연구결과 수상 운송업의 각종 유발계수로서 투입계수, 생산유발계수, 부가가치유발계수, 산업간 연쇄효과의 분석 결과를 제시 하였으며, 네트워크 연결성으로서 연결중앙성, 인접중앙성, 사이중앙성 및 네트워크 시각화 분석의 내용을 제시하였다. 본 연구를 통하여 수상 운송업의 현재 위치와 현황 및 전체 산업들과의 관계를 확인하였으며 어떠한 산업들과 전략적 관계를 가져야 하는가를 제시하였다. 향후에는 2000년대 이후 발간된 산업연관분석을 활용하여 각종 유발 효과 및 연결성(중앙성), 네트워크 시각화 분석의 변동 현황과 추이에 대한 사항을 추가로 분석하는 것이 필요하다.

• 한국식생활문화학회지
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• 제24권6호
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• pp.613-630
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• 2009

This thesis, which involves honest life stories of members of the ìtraditionalî Korean generation that lived through the turbulent times of the first half of the twentieth century, assesses the meaning and import of Korean cuisine during an individual Korean's lifetime, as well as the relevant properties of the culinary culture of the traditional generation and how those properties continue to influence the present generation of Koreans. Thus, traditional Korean culinary culture was subdivided into the following four aspects, each of which were exemplified by representative examples. The first of these is slow-food dietary life, which is exemplified by fermented foods. The development of side dishes (panchan) based on fermentation - kimchi, different types of soy and bean paste, salted seafoods, dishes of dried radish or cucumber slices seasoned with soy sauce, and so on - made the quantitative and qualitative supplementation of food possible for traditional Koreans. The second of these aspects, referred to as friendly dietary life, is exemplified by self-sufficiently produced foods. The system of many species and small production suitable with the season made it possible to produce food from sustainable ecological systems and to maintain locally grown food-cultures, each of which was distinguished from others by a local specialty product. The third aspect of the traditional Korean culinary culture involves the same use of medicinal roots and plant materials for foodstuff, and this is exemplified by the use of foods to cure and prevent diseases. The notion, for example, that 'boiled rice is an invigorant' is characteristic of the notion that diet can function in a preventative medical context, and other similar Korean notions illustrate the importance, also, of the curative properties of food. The fourth and final aspect of traditional Korean culinary culture identified herein is creative dietary life, which can be viewed essentially as a Korean adaptation to the turbulence of life during the early $20^{th}$ century in Korea. This trend is exemplified by many Korean foods that were created in response to foreign influences, such as onions, cabbages, curry, etc. which found their place in overall Korean culture through the age of Japanese settlement, as well as the Korean war.

• Geomechanics and Engineering
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• 제35권2호
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• pp.121-133
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• 2023

The demand for cement and limestone crushed materials has increased many folds due to the tremendous increase in construction activities in Pakistan during the past few decades. The number of cement production industries has increased correspondingly, and so the rock-blasting operations at the limestone quarry sites. However, the safety procedures warranted at these sites for the blast-induced ground vibrations (BIGV) have not been adequately developed and/or implemented. Proper prediction and monitoring of BIGV are necessary to ensure the safety of structures in the vicinity of these quarry sites. In this paper, an attempt has been made to predict BIGV using artificial neural network (ANN) at three selected limestone quarries of Pakistan. The ANN has been developed in Python using Keras with sequential model and dense layers. The hyper parameters and neurons in each of the activation layers has been optimized using randomized and grid search method. The input parameters for the model include distance, a maximum charge per delay (MCPD), depth of hole, burden, spacing, and number of blast holes, whereas, peak particle velocity (PPV) is taken as the only output parameter. A total of 110 blast vibrations datasets were recorded from three different limestone quarries. The dataset has been divided into 85% for neural network training, and 15% for testing of the network. A five-layer ANN is trained with Rectified Linear Unit (ReLU) activation function, Adam optimization algorithm with a learning rate of 0.001, and batch size of 32 with the topology of 6-32-32-256-1. The blast datasets were utilized to compare the performance of ANN, multivariate regression analysis (MVRA), and empirical predictors. The performance was evaluated using the coefficient of determination (R²), mean absolute error (MAE), mean squared error (MSE), mean absolute percentage error (MAPE), and root mean squared error (RMSE)for predicted and measured PPV. To determine the relative influence of each parameter on the PPV, sensitivity analyses were performed for all input parameters. The analyses reveal that ANN performs superior than MVRA and other empirical predictors, andthat83% PPV is affected by distance and MCPD while hole depth, number of blast holes, burden and spacing contribute for the remaining 17%. This research provides valuable insights into improving safety measures and ensuring the structural integrity of buildings near limestone quarry sites.

• Animal Bioscience
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• 제36권12호
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• pp.1775-1784
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• 2023

Objective: The aim of this study was to reveal the role and regulatory mechanism of miR-188-5p in the proliferation and differentiation of goat muscle satellite cells. Methods: Goat skeletal muscle satellite cells isolated in the pre-laboratory were used as the test material. First, the expression of miR-188-5p in goat muscle tissues at different developmental stages was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). In addition, miR-188-5p was transfected into goat skeletal muscle satellite cells by constructing mimics and inhibitors of miR-188-5p, respectively. The changes of differentiation marker gene expression were detected by qPCR method. Results: It was highly expressed in adult goat latissimus dorsi and leg muscles, goat fetal skeletal muscle, and at the differentiation stage of muscle satellite cells. Overexpression and interference of miR-188-5p showed that miR-188-5p inhibited the proliferation and promoted the differentiation of goat muscle satellite cells. Target gene prediction and dual luciferase assays showed that miR-188-5p could target the 3'untranslated region of the calcium/calmodulin dependent protein kinase II beta (CAMK2B) gene and inhibit luciferase activity. Further functional studies revealed that CAMK2B promoted the proliferation and inhibited the differentiation of goat muscle satellite cells, whereas si-CAMK2B restored the function of miR-188-5p inhibitor. Conclusion: These results suggest that miR-188-5p inhibits the proliferation and promotes the differentiation of goat muscle satellite cells by targeting CAMK2B. This study will provide a theoretical reference for future studies on the molecular mechanisms of skeletal muscle development in goats.

• 한국경제지리학회지
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• 제26권3호
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• pp.223-237
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• 2023

서울온수산업단지는 준공한 지 50년이 넘은 노후산업단지로, 산업단지 기반시설과 공장 건축물의 노후화가 진행중이다. 그럼에도 불구하고, 현재 서울온수산업단지에는 기계 산업 중심의 도시산업 집적이 발생하고 있다. 이 연구의 목적은 노후산업단지의 물리적 시설 노후화와 산업 집적 특성을 종합적으로 분석하여 서울온수산업단지의 정체성을 도출하는 데에 있다. 연구 결과, 서울온수산업단지는 수도권에 집중된 거래 네트워크, 소필지화로 인한 영세기업의 입주, 소음 유발 공정의 허용, 고숙련 기술자 확보 용이성에 따라 도시산업 집적이 강화되고 있다. 그러나 이 집적은 산업단지 생산기능의 약화, 입주기업의 제한된 혁신성, 수도권 기계 산업경쟁력 약화에 따른 비혁신적 특성이 공존하는 것으로 나타났다. 이를 종합하면 서울온수산업단지의 정체성은 '비혁신형 도시산업 집적지'로, 수도권 중심의 기계 산업 네트워크에 기반한 비혁신적 집적이 발생하고 있는 노후산업단지이다.

• 한국경제지리학회지
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• 제26권4호
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• pp.458-476
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• 2023

본 연구는 공유지 비극의 극복 방안으로 공동체 자치 모델에 대한 기존 사례 연구를 분석하여 성공적인 공유지 관리의 필요 요소와 방해 요소를 도출한다. 이러한 요소를 성공적 공동체 자치 모델로 인정받는 제주해녀 공동체의 사회·경제적 속성과 비교 분석하여, 현재 당면한 내·외부적 변화 속에서 앞으로도 이러한 공동체 내부 기제가 유용하게 작동될 수 있을 것인지, 향후 제주해녀 공동체 자치의 지속에 필요한 사회·경제적 요소는 무엇인지 밝히는 것을 목적으로 하고 있다. 기존 사례에서 도출된 공동체 자치의 성공과 실패 요인에 따라 제주해녀 공동체 내부 작동 기제를 분석한 결과, 신뢰와 호혜성을 향상시키는 생산과 분배체계, 내재된 공동체 의식, 공식·비공식 조직의 역할 분담과 조직 내 제도화된 명시적, 암묵적 규범은 지속가능성의 내·외부 강점으로 작용했다. 그러나 네트워크의 폐쇄성, 생산성의 위기, 동질성의 약화와 새로운 주체의 등장은 내·외부 약점으로 작용했다. 결론적으로 향후 공유지 관리를 위한 제주해녀 공동체의 지속가능성을 위해서는 해녀학교를 활용한 노동의 재생산 기능의 재정비, 공동체 생업 및 문화 전승 주체의 명확성과 해녀 중심의 권한 유지, 그리고 해녀들의 기타소득 보장 방안 마련이 필요하다.

• Journal of Applied Biological Chemistry
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• 제66권
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• pp.394-403
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• 2023

지속 가능하고 친환경적인 농업 관행을 추구하기 위해 우리는 40년이 넘는 장기간 동안 화학 비료를 사용하지 않은 토양에 서식하는 근권 박테리아에 대한 광범위한 연구를 수행하였다. 이번 조사를 통해 식물생장촉진 근권박테리아 총 80종을 분리하고 이들의 식물생장 증진 가능성을 평가했다. 이러한 분리균중에서 Burkholderia cepacia CD2는 가장 우수한 식물 성장촉진 활성과 생장능을 나타내어 추가 분석을 위한 최적의 후보균주로 선정되었다. Burkholderia cepacia CD2는 인 가용화 능력, 사이드로포어 생산, 탈질화 능력, 아질산 이온 활용능력 및 요소분해효소 활성을 포함하여 식물 성장에 도움이 되는 다양한 유익한 특성을 나타내었다. 이러한 특성은 식물의 성장과 발달에 긍정적인 영향을 미치는 것으로 잘 알려져 있다. 균주의 분류학적 분류를 검증하기 위해 16S rRNA 유전자 서열분석을 통해 Burkholderia 속 내 위치를 확인하여 계통발생 관계에 대한 추가 통찰력을 제공하였다. 식물 생장 촉진 특성의 기본 메커니즘을 더 깊이 조사하기 위해 우리는 CD2에서 식물 생장촉진과 관련된 특정 유전자의 존재를 확인하려고 하였다. 이를 달성하기 위해 옥스포드 나노포어를 활용하여 전장 유전체 시퀀싱을 수행하였다. CD2 게놈에 대한 전장유전체 분석을 통해 식물 생장 개선에 중추적 요인으로 생각되는 하위 시스템 기능을 확인하였다. 이러한 발견을 바탕으로 Burkholderia cepacia CD2는 미생물 비료로 작용하여 화학 비료에 대한 지속 가능한 대안을 제공할 수 있는 잠재력을 가지고 있다는 결론을 내릴수 있다.

• 농촌계획
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• 제30권1호
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• pp.15-31
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• 2024

The study primarily aims to examine the characteristics of the transition from natural landscape to modern agricultural landscape on the Daesan plain in Dong-myeon, Changwon-si, in the lower reaches of the Nakdong River. The periods covered in the transition include the late Joseon Dynasty, the early Japanese colonial period, and the late Japanese colonial period. The study concluded the following: It was found that the Daesan Plain used to function as a hydrophilic landscape before it formed into a rural landscape. This is characterized by the various water resources in the Plain, primarily by the Nakdong River, with its back marsh tributaries, the Junam Reservoir and Jucheon. To achieve its recent form, the Daesan Plain was subjected to human trial and error. Through installation of irrigation facilities such as embankments and sluices, the irregularly-shaped wetlands were transformed into large-scale farmlands while the same irrigation facilities underwent constant renovation to permanently stabilize the rural landscape. These processes of transformation were similarly a product of typical colonial expropriation. During the Japanese colonial period, Japanese capitalists initiated the construction of private farms which led to the national land development policy by the Governor-General of Korea. These landscape changes are indicative of resource capitalism depicted by the expansion of agricultural production value by the application of resource capital to undeveloped natural space for economic viability. As a result, the hierarchical structure was magnified resulting to the exacerbation of community and economic structural imbalances which presents an alternative yet related perspective to the evolution of landscapes during the Japanese colonial period. In addition, considering Daesan Plain's vulnerability to changing weather conditions, natural processes have also been a factor to its landscape transformation. Such occurrences endanger the sustainability of the area as when floods inundate cultivated lands and render them unstable, endangering residents, as well as the harvests. In conclusion, the Daesan Plain originally took the form of a hydrophilic landscape and started significantly evolving into a rural landscape since the Japanese colonial period. Human-induced land development and geophysical processes significantly impacted this transformation which also exemplifies the several ways of how undeveloped natural landscapes turn into mechanized and capitalized rural landscapes by colonial resource capitalism and development policies.