• 한국미생물·생명공학회지
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• 제46권2호
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• pp.135-144
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• 2018

The rapid increase in number and diversity of Extended Spectrum ${\beta}$-Lactamases (ESBLs) producing Enterobacteriaceae in natural aquatic environment is a major health concern worldwide. This study investigates abundance and distribution of ESBL producing multidrug resistant Enterobacteriaceae and molecular characterization of ESBL genes among isolates from highly polluted stretch of river Yamuna, India. Water samples were collected from ten different sites distributed across Delhi stretch of river Yamuna, during 2014-15. A total of 506 non duplicate Enterobacteriaceae isolates were obtained. Phenotypic detection of ESBL production and antibiotic sensitivity for 15 different antibiotics were performed according to CLSI guidelines (Clinical and Laboratory Standard Institute, 2015). A subset of ESBL positive Enterobacteriaceae isolates were identified by 16S rRNA gene and screened for ESBL genes, such as $bla_{CTX-M}$, $bla_{TEM}$ and $bla_{OXA}$. Out of 506 non-duplicate bacterial isolates obtained, 175 (34.58%) were positive for ESBL production. Susceptibility pattern for fifteen antibiotics used in this study revealed higher resistance to cefazolin, rifampicin and ampicillin. A high proportion (76.57%) of ESBL positive isolates showed multidrug resistance phenotype, with MAR index of 0.39 at Buddha Vihar and Old Delhi Railway bridge sampling site. Identification and PCR based characterization of ESBL genes revealed the prevalence of $bla_{CTX-M}$ and $bla_{TEM}$ genes to be 88.33% and 61.66%, respectively. Co-occurrence of $bla_{CTX-M}$ and $bla_{TEM}$ genes was detected in 58.33% of the resistant bacteria. The $bla_{OXA}$ gene was not detected in any isolates. This study highlights deteriorating condition of urban aquatic environment due to rising level of ESBL producing Enterobacteriaceae with multidrug resistance phenotype.

• Journal of Microbiology and Biotechnology
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• 제26권7호
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• pp.1290-1302
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• 2016

Microalgae are being researched for their potential as attractive biofuel feedstock, particularly for their lipid production. For maximizing biofuel production, it is necessary to explore the effects of environmental factors on algal lipid-producing potential. In this study, the effects of nitrogen (N) sources (NO₂-N, NO₃-N, urea-N, NH₄-N, and N-deficiency) and carbon-to-nitrogen ratios (C/N= 0, 1.0, 3.0, and 5.0) on algal lipid-producing potential of Chlorella sp. HQ were investigated. The results showed that for Chlorella growth and lipid accumulation potential, NO₂-N was the best amongst the nitrogen sources, and NO₃-N and urea-N also contributed to algal growth and lipid accumulation potential, but NH₄-N and N-deficiency instead caused inhibitory effects. Moreover, the results indicated that algal lipid-producing potential was related to C/N ratios. With NO₂-N treatment and carbon addition (C/N = 1.0, 3.0, and 5.0), total lipid yield was enhanced by 12.96-20.37%, but triacylglycerol (TAG) yields decreased by 25.52-94.31%. As for NO₃-N treatment, carbon addition led to a 17.82-57.43%/25.86-82.67% reduction of total lipid/TAG yields. When NH₄-N was used as the nitrogen source, total lipid/TAG yields were increased by 46.67-113.33%/28.99-74.76% with carbon addition. The total lipid/TAG yields of urea-N treatment varied with C/N ratios. Overall, the highest TAG yield (TAG yield: 38.75 ± 5.21 mg/l; TAG content: 44.16 ± 4.35%) was achieved under NO₂-N treatment without carbon addition (C/N = 0), the condition that had merit for biofuel production.

• 한국환경과학회지
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• 제12권4호
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• pp.503-510
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• 2003

How to plan the energy system is one of the keys f3r constructing the Environment -Friendly City. for this reason, a great number of surveys for utilizing unused energy have conducted by a planner. In regard to unused energy, the heat from incineration plants classify as a unused energy having high-exergy-energy. From this point of view, It is studied about the plant systems providing heat to district heating & cooling(D.H.C) and producing electric power. It is divided four system models as system I (10K [kgf/cm$^2$) vapor as outlet of boiler, supply far 10K vapor and return to 60$^{\circ}C$ as supply condition of district heating), system II (30 K vapor as outlet of boiler, supply for 5t vapor and return to 60f as supply condition of district heating), system 111 (30 K vapor as outlet of boiler, supply for 85$^{\circ}C$ hot water and return to 60$^{\circ}C$ as supply condition of district heating), system IV (30 K vapor as outlet of boiler, supply for 47$^{\circ}C$ hot water and return to 40t as supply condition of district heating). The results from the upper condition of four system, System II got a proper on economical benefits and system IV calculated as benefiting on energy saving effects, and suggest indifference curve as the total evaluation method of both economical benefits and energy saving.

• 조명전기설비학회논문지
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• 제27권3호
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• pp.65-78
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• 2013

To analyze influences under open fault conditions in switching devices, an integrated simulation and condition monitoring scheme for a permanent magnet synchronous generator (PMSG) based variable speed grid-connected wind turbine system are presented. Among various faults in power electronics components, the open fault in switching devices may arise when the switches are destructed by an accidental over current, or a fuse for short protection is blown out. Under such a faulty condition, the grid-side inverter as well as the generator-side converter does not operate normally, producing an increase of current harmonics, and a reduction in output and efficiency. As an effective way for a condition monitoring of generation system by online basis without requiring any diagnostic apparatus, the estimation schemes for generated voltage, flux linkage, and stator resistance are proposed and the validity of the proposed scheme is proved through comparative simulations.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2006년도 춘계학술대회
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• pp.478-481
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• 2006

Energy is vital to global prosperity, yet dependence on fossil fuels as our primary energy source contributes to global climate change environmental degradation, and health problems. Hydrogen $(H_2)$ offers tremendous potential as a clean renewable energy currency. Hydrogen has the highest gravimetric energy density of any known fuel and is compatible with electrochemical and combustion processes for energy conversion without producing carbon-based emission that contribute to environmental pollution and climate change. Numerous methodologies have been developed for effective hydrogen production. Among them, the biological hydrogen production has gained attention, because hydrogen can be produced by cellular metabolismunder the presence of water and sunlight. The green alga Chlamydomonas reinhardtii is capable of sustained $H_2$ photoproduction when grown under sulfur deprived condition. Under sulfur deprived conditions, PSII and photosynthetic $O_2$ evolution are inactivated, resulting in shift from aerobic to anaerobic condition in the culture. After anaerobiosis, sulfur deprived algal cells induce a reversible hydrogenase and start to evolve $H_2$ gas in the light. According to above principle, we investigated the effect of induction parameters such as cell age, cell density. light intensity, and sulfate concentration under sulfur deprived condition We also developed continuous hydrogen production system by sulfate re-addition under sulfur deprived condition.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 1994년도 가을 학술발표회 논문집
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• pp.137-142
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• 1994

This study is aimed for producing the high strength Re-Mi-Con about 400kg/$\textrm{cm}^2$ with Fly-Ash in consideration of the field condition and material in practice. Test results show that compressive strength for 28 days is 300~470kg/$\textrm{cm}^2$ within 30~40% of W/C according to the 15~21cm of target slump.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2005년도 International Meeting on Information Displayvol.II
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• pp.1252-1254
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• 2005

Recently, PDP barrier ribs require the formation of complex structure so that they are usually formed by etching method. For producing the fine ribs structure, during the etching process the metal ions of matrix (glass) of barrier materials should be understood on the etching mechanism with etching condition. We analyzed the quantity of Pb, Si, and B ions from the etch solution as a function of etching time.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1989년도 추계학술대회 논문집 학회본부
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• pp.5-9
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• 1989

In this paper, while 2-or 3-phase induction motor is driven with balance and no noise driving at dynamic and steady state condition. Single-phase induction motor is driven with pulsating torque except specific driving point. So we analyze the reason of producing pulsating torque with the view of unsymmetring concept. These results are applied to the design of high efficincy, high quality single-phase induction motor.

• 기술혁신연구
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• 제9권2호
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• pp.98-119
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• 2001

The purpose of this study is to classify the world countries into several cluste on the basis of the characteristics of the scientific and technological development, and analyse the main factors of development in each cluster. Major contributions of this study can be summarized as follows: (1) In the process of producing the composite indicators, individual indicators were adopted only if they met the condition which were logically compliable for the given time periods. Hence this thesis supports the reliability in comparing results of analysis in each period of years. (2) Could clearly capture the development characteristics and its changing process in each country.

• 한국식품영양학회지
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• 제13권2호
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• pp.171-175
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• 2000

Microbial proteases have certain unique characteristics, and are now widely used in food, leather, detergent, and pharmaceutical industries. Thermophilic Actinomyces producing the protease was isolated from soil in Wonju city. This strain was able to grow and produce protease at the culture temperature of 50$^{\circ}C$. The maximum protease production was obtained when 0.5% soluble starch and 0.4% yeast extract were used as carbon and nitrogen source, respectively. The other culture condition for the maximal productivity of the protease was 0.1% K2HPO4, and 0.05% CaCl2 at initial pH 8.0 for 48 hours.