• Journal of Applied Biological Chemistry
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• 제49권2호
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• pp.39-47
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• 2006

Incorporation of bioactive compounds such as vitamins, probiotics, bioactive peptides, and antioxidants into Nutrient Delivery System (NDS) for 'nanofood' provides simple way to develop novel functional foods that may have physiological benefits or reduce risks of diseases. As vital nutrient in nanofood, proteins possess unique functional properties including ability to form gels and emulsions, which allow them to be ideal nanofood materials for encapsulation of bioactive compounds. Based on protein physico-chemical properties, this review describes potential role of nanofood materials for development of NDS in hydrogel form, micro-or nano-particles. Applications of these nanofood materials to protect delivery-sensitive nutraceutical compounds are illustrated, and impacts of particle size on release properties are emphasized.

• Journal of Microbiology and Biotechnology
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• 제22권12호
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• pp.1597-1604
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• 2012

Genus Bacillus is a spore-forming bacterium that has unique properties in cell differentiation, allowing the forming of spores in stress conditions and activated in the vegetative cell, with suitable environments occurring during the life cycle acting as a trigger. Their habitat is mainly in soil; thus, many species of Bacillus are associated with plants as well as rhizosphere bacteria and endophytic bacteria. Signal transduction is the principal mechanism of interactions, both within the cell community and with the external environment, which provides the subsequent functions or properties for the cell. The antimicrobial compounds of Bacillus sp. are potentially useful products, which have been used in agriculture for the inhibition of phytopathogens, for the stimulation of plant growth, and in the food industry as probiotics. There are two systems for the synthesis of these substances: nonribosomal synthesis of cyclic lipopeptides (NRPS) and polyketides (PKS). For each group, the structures, properties, and genes of the main products are described. The different compounds described and the way in which they co-exist exhibit the relationship of Bacillus substances to plants, humans, and animals.

• 대한수의학회지
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• 제44권4호
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• pp.581-586
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• 2004

The growth characteristics and the properties of the Enterococcus faecium (E. faecium) isolated from intestine of slaughting pig were examined in order to confirm whether it will be used practically as probiotics or not. E. faecium was identified by morphological and biochemical tests including of final confirming by API strep kit. Among 264 samples, 44 isolates (16.7%) of E. faecium were observed as an exellent growth as a range of $1{\times}10^7CFU/ml{\sim}3.3{\times}10^9CFU/ml$ in viable bacterial count on MRI medium. All isolates were shown non-haemolysis on the blood agar exception of 28 isolates shown ${\alpha}$-haemolysis and were identified as E. faecium by biochemical test using API strep enzyme kits. Eight strains (18.2%) were finally selected from which they were excellent sensitivity in showing of the acid-tolerance and the bile-tolerance in compare with reference strain.

• 한국축산식품학회지
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• 제36권2호
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• pp.267-274
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• 2016

The purposes of this study were to investigate the impacts of concentration levels of whey protein isolate (WPI) and inulin on the formation and physicochemical properties of WPI/inulin nano complexes and to evaluate their potential prebiotic effects. WPI/inulin nano complexes were produced using the internal gelation method. Transmission electron microscopy (TEM) and particle size analyzer were used to assess the morphological and physicochemical characterizations of nano complexes, respectively. The encapsulation efficiency of resveratrol in nano complexes was studied using HPLC while the potential prebiotic effects were investigated by measuring the viability of probiotics. In TEM micrographs, the globular forms of nano complexes in the range of 10 and 100 nm were successfully manufactured. An increase in WPI concentration level from 1 to 3% (w/v) resulted in a significant (p<0.05) decrease in the size of nano complexs while inulin concentration level did not affect the size of nano complexes. The polydispersity index of nano complexes was below 0.3 in all cases while the zeta-potential values in the range of -2 and -12 mV were observed. The encapsulation efficiency of resveratrol was significantly (p<0.05) increased as WPI and inulin concentration levels were increased from 1 to 3% (w/v). During incubation at 37℃ for 24 h, WPI/inulin nano complexes exhibited similar viability of probiotics with free inulin and had significantly (p<0.05) higher viability than negative control. In conclusions, WPI and inulin concentration levels were key factors affecting the physicochemical properties of WPI/inulin nano complexes and had potential prebiotic effect.

• 농업생명과학연구
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• 제46권4호
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• pp.119-127
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• 2012

To investigate the influence of multi-probiotic, fermented ginseng byproduct and fermented sulfone on the performance, intestinal microflora and immunity of broiler, a five weeks trial was conducted with 340, 1-d-old $Ross{\times}Ross$ broiler. All broilers were divided into five different groups having 68 birds in each treatment, and they were assigned as control, antibiotic avilamycin (AB), multi-probiotic (MP), fermented sulfone (FS) and fermented ginseng byproduct (FGB). Each artificial or naturally derived probiotic was inoculated 0.1% level with the basal diet, and all diets were provided to birds for five weeks. Weight gain and feed intake were measured weekly basis, and blood, spleen and feces were collectedand used for the physiological properties of broiler chickens. All performances and cholesterol profiles were not significantly differed but numerically lower level of neutral fat and LDL was found in multi-probiotics and FGB treatments respectively. The salmonella spp and E. coli numbers in the ileum were high in control in relation to those of other treatments and were significantly decreased in antibiotics treatments (p<0.05). In addition, Lactobacillus spp. showed significantly higher proliferation in MP as compared to that of others (p<0.05). Fecal ammonia and $CO_2$ gas emission was significantly decreased in MP, FGB and FS, respectively (p<0.05), but significantly increased proliferation of spleen was determined in MP group in comparison of other treatments (p<0.05). Therefore, the results indicates that multi-probiotics would be valuable feed additives to improve the salmonella, E. coli and Lactobacillus proliferation, and manure gas emission of broiler chickens, but further study related to the production of manure gas emission of MP is necessary.

• Journal of Microbiology and Biotechnology
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• 제29권7호
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• pp.1022-1032
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• 2019

Probiotics are known to provide the host with immune-modulatory effects and are therefore of remarkable interest for therapeutic and prophylactic applications against various disorders, including inflammatory diseases. Weissella cibaria JW15 (JW15) has been reported to possess probiotic and antioxidant properties. However, the effect of JW15 on inflammatory responses has not yet been reported. Therefore, the objective of the current study was to evaluate the anti-inflammatory potential of JW15 against lipopolysaccharide (LPS) stimulation. The production of pro-inflammatory factors and the cellular signaling pathways following treatment with heat-killed JW15 was examined in LPS-induced RAW 264.7 cells. Treatment with heat-killed JW15 decreased nitric oxide and prostaglandin $E_2$ production via down-regulation of the inducible nitric oxide synthase and cyclooxygenase-2. In addition, treatment with heat-killed JW15 suppressed the expression of pro-inflammatory cytokines, interleukin $(IL)-1{\beta}$, IL-6, and tumor necrosis factor-${\alpha}$. The anti-inflammatory properties of treating with heat-killed JW15 were associated with mitogen-activated protein kinase signaling pathway-mediated suppression of nuclear factor-${\kappa}B$. These results indicated that JW15 possesses anti-inflammatory potential and provide a molecular basis regarding the development of functional probiotic products.

• 한국축산시설환경학회지
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• 제8권1호
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• pp.35-42
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• 2002

본 시험은 미생물제제를 이용한 발효돈분 액비를 질소 함량을 기준으로 하여 수수$\times$수단그라스 교잡종 재비시 사용하였을 때 생육, 생산성 및 토양에 미치는 영향을 구명하고자 3처리(화학비료 시용구, 일반발효돈분비액비 시용구, 미생물제제 이용 발효돈분액비 시용구)를 난괴법 3반복으로 배치하여 제주지역에서 수행하였다. 파종후 생육초기에는 초장이 일반발효돈분액비 시용구가 화학비료 시용구에 비하여 다소 길었으나. 수확시에는 처리간 차이없이 비슷하였다. ha당 총 건물수량은 41.848~15.421kg으로 처리간 유의적인 차이는 없었다. 또한 수수교잡종의 CP, NDF, ADF 및 무기물 함량도 처리간 큰 차이없이 비슷한 경향이였다. 시험후 토양의 pH는 5.35~5.63으로 비슷하여 유의적인 차니는 보이지 않았으나 유효인산 함량은 화학비료 시용구가 발효돈분액비 시용구들에 비해 높았다(P<0.05). 치환성 양이온 중 K는 미생물제제 이용 발효돈분액비를 시용하였을 때 유의적으로 높은 함량을 보였으나 (p<0.05), 2차 수확후에는 차이가 없었다.

• 한국축산식품학회지
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• 제29권1호
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• pp.114-120
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• 2009

총 50두의 돼지를 출하하기 전 55일 동안 일반 사료에 0.3% 들국화분말, 0.1% 생균제, 0.1% 셀레늄을 급여한 시험구 돼지의 육질특성을 조사하였다. 대조구에 비하여 일반성분, 콜레스테롤함량, 이화학적 특성 및 육색의 변화에 유의적인 차이가 나타나지 않았다. 조직감의 경우 경도는 유의적으로 높았으나 파쇄성, 응집성, 탄력성, 검성 및 씹힘성에는 유의적인 차이가 나타나지 않았다. 관능검사의 경우 신선육의 종합적인 기호도, 조리육의 연도, 다즙성 및 종합적기호도가 대조구보다 높게 나타났으나, 신선육의 육색, 드립 및 마블링 지수, 조리육의 육색, 육향 및 맛에서는 유의적인 차이가 나타나지 않았다. 지방산 조성의 경우 처리구의 myristic acid, palmitoleic acid, oleic acid, unsaturated fatty acid(UFA), MUFA(monounsaturated fatty acid), MUFA/SFA(saturated fatty acid) 및 n-6/n-3 ratio가 높게 나타났으나, palmitic acid, stearic acid, linolenic acid, arachidonic acid, SFA, essential fatty acid(EFA)의 지방산의 함량은 낮게 나타났다.

• 생명과학회지
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• 제33권4호
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• pp.334-342
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• 2023

가자미식해에서 분리된 유산균 40종은 Lactobacillus plantarum spp., Leuconostoc mesenteroides spp., Lactobacillus brevis spp., and Weisella paramenteroides spp.로 확인됐으며, 40종 중 10종을 선별해 검사에 사용했다. 본 연구에서는 가자미식해에서 분리된 유산균을 프로바이오틱스로 활용하기 위해 산 및 인공위액 저항성, 담즙산 저항성, 자가 응집성, 공동 응집성, 세포 표면 소수성 등의 프로바이오틱 연구를 수행하였다. 분리한 유산균주는 산성 및 인공 위액에 대해 저항성을 보여 높은 생존율을 나타내었으며, L. plantarum GS11이 가장 뛰어난 저항성을 보였다. 또한 담즙산 저항성 측정 결과 모든 유산균주가 10⁸~10⁹ log CFU/ml의 생균수로, 100% 이상의 생존력을 보여주었다. 그리고 세포 표면 부착능을 간접적으로 측정하기 위해 응집력 평가를 한 결과, autoaggregation 능력을 46% 이상 나타내었다. 세포 표면 소수성 평가를 위해 Xylene 부착능을 측정한 결과 분리된 유산균주에서 32.2%의 소수성을 가지는 B. subtilis 보다 더 뛰어난 세포 부착률을 보여주었다. 이와같이 유산균은 프로바이오틱스로 활용될 만한 유의미한 결과를 보였으며, L. plantarum GS12와 L. plantarum GS13을 제외한 유산균에서 항균 활성이 나타났다. 따라서 가자미 식해에서 분리된 유산균은 다양한 프로바이오틱스 특성을 가진 프로바이오틱스로 활용할 수 있을 것으로 판단되어진다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권5호
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• pp.751-755
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• 2006

Dadih is Indonesian traditional fermented buffalo milk believed by the natives to have beneficial effects on human health. This may be due to the probiotic properties possessed by the lactic acid bacteria (LAB) involved in its fermentation process. It was discovered that ten strains of dadih lactic isolates possessed some probiotic properties in vitro. In this study, the adhesion properties of dadih LAB, in comparison with documented probiotic strains, were investigated in vitro by using mucin extracted from human faeces and Caco-2 cells as the models for human intestinal mucosal surface and intestinal cells respectively. The adhesion results showed the distinction of Lactobacillus reuteri IS-27560 in adhering to both mucus layer and Caco-2 cells. The competition assay for adhesion to the mucus layer between dadih LAB and selected pathogens indicated the competence of Lactococcus lactis IS-16183 and Lactobacillus rhamnosus IS-7257 in significantly inhibiting the adhesion of Escherichia coli O157:H7. Accordingly, these two strains may be potential candidates for use as probiotic strains. Overall, the adhesion properties of all dadih LAB strains were relatively comparable to that of Lactobacillus casei Shirota and Lactobacillus rhamnosus GG, the documented probiotic strains.