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http://dx.doi.org/10.5851/kosfa.2016.36.2.267

Physicochemical Characterization and Potential Prebiotic Effect of Whey Protein Isolate/Inulin Nano Complex  

Ha, Ho-Kyung (Department of Animal Bioscience and Institute of Agriculture and Life Science, Gyeongsang National University)
Jeon, Na-Eun (Department of Animal Bioscience and Institute of Agriculture and Life Science, Gyeongsang National University)
Kim, Jin Wook (Department of Animal Bioscience and Institute of Agriculture and Life Science, Gyeongsang National University)
Han, Kyoung-Sik (Department of Animal Biotechnology and Resource, Sahmyook University)
Yun, Sung Seob (R&D Center, Edam Co., Ltd)
Lee, Mee-Ryung (Department of Food and Nutrition, Daegu University)
Lee, Won-Jae (Department of Animal Bioscience and Institute of Agriculture and Life Science, Gyeongsang National University)
Publication Information
Food Science of Animal Resources / v.36, no.2, 2016 , pp. 267-274 More about this Journal
Abstract
The purposes of this study were to investigate the impacts of concentration levels of whey protein isolate (WPI) and inulin on the formation and physicochemical properties of WPI/inulin nano complexes and to evaluate their potential prebiotic effects. WPI/inulin nano complexes were produced using the internal gelation method. Transmission electron microscopy (TEM) and particle size analyzer were used to assess the morphological and physicochemical characterizations of nano complexes, respectively. The encapsulation efficiency of resveratrol in nano complexes was studied using HPLC while the potential prebiotic effects were investigated by measuring the viability of probiotics. In TEM micrographs, the globular forms of nano complexes in the range of 10 and 100 nm were successfully manufactured. An increase in WPI concentration level from 1 to 3% (w/v) resulted in a significant (p<0.05) decrease in the size of nano complexs while inulin concentration level did not affect the size of nano complexes. The polydispersity index of nano complexes was below 0.3 in all cases while the zeta-potential values in the range of -2 and -12 mV were observed. The encapsulation efficiency of resveratrol was significantly (p<0.05) increased as WPI and inulin concentration levels were increased from 1 to 3% (w/v). During incubation at 37℃ for 24 h, WPI/inulin nano complexes exhibited similar viability of probiotics with free inulin and had significantly (p<0.05) higher viability than negative control. In conclusions, WPI and inulin concentration levels were key factors affecting the physicochemical properties of WPI/inulin nano complexes and had potential prebiotic effect.
Keywords
nano complex; whey protein isolate (WPI); inulin; prebiotic effect;
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1 Aryana, K. J., Plauche, S., Rao, R. M., McGrew, P., and Shah, N. P. (2007) Fat-free plain yogurt manufactured with inulins of various chain lengths and Lactobacillus acidophilus. J Food Sci. 72, M79-M84.   DOI
2 Bruno, F. A., Lankaputhra, W. E. V., and Shah, N. P. (2002) Growth, viability and activity of Bifidobacterium ssp. in skim milk containing prebiotics. J Food Sci. 67, 2740-2744.   DOI
3 Bryant, C. M. and McClements, D. J. (2000) Influence of NaCl and CaCl2 on cold-set gelation of heat-denatured whey protein. J. Food Sci. 65, 801-804.   DOI
4 Chen, L. and Subirade, M. (2005) Chitosan/β-lactoglobulin core-shell nanoparticles as nutraceutical carriers. Biomater. 26, 6041-6053.   DOI
5 Fathi, M., Mozafari, M. R., and Mohebbi, M. (2012) Nanoencapsulation of food ingredients using lipid based delivery systems. Trends Food Sci. Tech. 23, 13-27.   DOI
6 Das, S. and NG, K. Y. (2010) Resveratrol-loaded calciumpectinate beads: Effects of formulation parameters on drug release and bead characteristics. J. Pharm. Sci. 99, 840-860.   DOI
7 Demetriades, K., Coupland, J. N., and McClements, D. J. (1997) Physicochemical properties of whey protein-stabilized emulsions as affected by heating and ionic strength. J. Food Sci. 62, 462-467.   DOI
8 Donkor, O. N., Nilmini, S. L. I., Stolic, P., Vasiljevic, T., and Shah, N. P. (2007) Survival and activity of selected probiotic organisms in set-type yoghurt during cold storage. Int. Dairy J. 17, 657-665.   DOI
9 Fioramonti, S. A. Perez, A. A., Aríngoli, E. E., Rubiolo, A. C., and Santiago, L. G. (2014) Design and characterization of soluble biopolymer complexes produced by electrostatic self-assembly of a whey protein isolate and sodium alginate. Food Hydrocolloid. 35, 129-136.   DOI
10 Gilbowski, P. (2009) Rheological properties and structure of inulin-whey protein gels. Int. Dairy J. 19, 443-449.   DOI
11 Gilbowski, P. and Gilbowska, A. (2009) Effect of calcium chloride on rheological properties and structure of inulin-whey protein gels. World Acad. Sci. Eng. Technol. 27, 813-817.
12 Ha, H. K., Kim, J. W., Lee, M. R., and Lee, W. J. (2013) Formation and characterization of quercetin-loaded chitosan oligosaccharide/ β-lactoglobulin nanoparticle. Food Res. Int. 52, 82-90.   DOI
13 He, J. S. and Ruan, K. (2009) Kinetics of phase separation during pressure-induced gelation of a whey protein isolate. Food Hydrocolloid. 23, 1729-1733.   DOI
14 Koh, J. H., Choi, S. H., Park, S. W., Choi, N. J., Kim, Y., and Kim, S. H. (2013) Synbiotic impact of tagatose on viability of Lactobacillus rhamnosus strain GG mediated by the phosphotransferase system (PTS). Food Microbiol. 36, 7-13.   DOI
15 Leclerc, P.-L., Remondetto, G. E., Ramassamy, C., and Subirade, M. (2005) Whey protein nanospheres as drug carriers for oral administration. Conference on bioencapsulation, Kingston, pp. 24-26.
16 Hu, B., Pan, C., Sun, Y., Hou, Z., Ye, H., Hu, B., and Zeng, X. (2008) Optimization of fabrication parameters to produce chitosan-tripolyphosphate nanoparticles for delivery of tea catechins. J. Agric. Food Chem. 56, 7451-7458.   DOI
17 Keowmaneechai, E. and McClements, D. J. (2002) Effect of CaCl2 and KCl on physicochemical properties of model nutritional beverages based on whey protein stabilized oil-in-water emulsions. J. Food Sci. 67, 665-671.   DOI
18 Lee, M. R., Choi, H. N., Ha, H. K., and Lee, W. J. (2013) Production and characterization of β-lactoglobulin/alginate nanoemulsion containing coenzyme Q10: Impact of heat treatment and alginate concentrate. Korean J. Food Sci. An. 33, 67-74.   DOI
19 Lee, M. R., Nam, G. W., Choi, H. N., Yun, H. S., Kim, S. H., You, S. K., Park, D. J., and Lee, W. J. (2008) Structure and chemical properties of beta-lactoglobulin nanoparticles. J. Agric. Life Sci. 42, 31-36.
20 Liang, L., Tajmir-Riahi, H. A., and Subirade, M. (2008) Interaction of beta-lactoglobulin with resveratrol and its biological implications. Biomacromolecules 9, 50-56.   DOI
21 Livney, Y. D. (2010) Milk proteins as vehicles for bioactives. Curr. Opin. Colloid Interface Sci. 15, 73-83.   DOI
22 Sessa, M., Balestrieri, M. L., Ferrari, G., Servillo, L., Castaldo, D., D’Onofrio, N., Donsi, F., and Tsao, R. (2014) Bioavailability of encapsulated resveratrol into nanoemulsion-based delivery systems. Food Chem. 147, 42-50.   DOI
23 Tobin, J. T., Fitzsimons, S. M., Kelly, A. L. Kelly, P. M., Auty, M. A. E., and Fenelon, M. A. (2010) Microparticulation of mixtures of whey protein and inulin. Int. J. Dairy Tech. 63, 32-40.   DOI
24 Ron, N., Zimet, P., Bargarum, J., and Livney, Y. D. (2010) β- lactoglobulin-polysaccharide complexes as nanovehicles for hydrophobic nutraceuticals in non-fat foods and clear beverages. Int. Dairy J. 20, 686-693.   DOI
25 Schaller-Povolny, L. A. and Smith, D. E. (2002) Interaction of milk proteins with inulin. Milchwissenschaft. 57, 494-497.
26 Zimet, P. and Livney, Y. D. (2009) Beta-lactoglobulin and its nanocomplexes with pectin as vehicles for ω-3 polyunsaturated fatty acids. Food Hydrocolloid. 23, 1120-1126.   DOI