• 한국산학기술학회논문지
• /
• 제12권6호
• /
• pp.2652-2659
• /
• 2011

생리활성, 물성 및 관능적인 특성 등이 개선된 발효빵의 개발을 위하여 복합 생균제와 제빵효모의 적정 사용량 규명을 위한 연구를 수행하였다. 복합 생균제와 제빵효모(Saccharomyces cerevisiae)의 초기 사용량은 각각 1.5 : 0.1(Test 1군), 0.3 : 0.02(Test 2군), 0.15 : 0.01(g/g)(Test 3군)였다. 복합 생균제의 사용으로 반죽내의 생균제 균수는 44~642배 증가하였으며, 반죽의 pH는 산성화 되었다. 복합 생균제를 사용한 반죽의 pH와 비교시, 제빵효모를 사용하여 15시간 발효한 반죽의 pH는 상대적으로 높게 나타났다. 스타터균의 사용량을 달리한 세 가지 그룹들 중에서, 빵의 부피와 경도, 식빵껍질의 두께, 기공의 형태, 압박력 지수, 빵의 비용적 등에서 유의적인 차이가 없었다. 그러나, 관능평가에서는 발효빵의 향, 맛 및 전체적인 기호도는 Test 2군에서 유의적으로 높게 나타났다(p<05). 결과적으로, Test 1군과 Test 3군들보다 Test 2군에서 우수한 제빵특성을 보였다.

• 동의생리병리학회지
• /
• 제27권1호
• /
• pp.107-111
• /
• 2013

To investigate the possible usage as probiotics, we isolated and identified one strain of bacillus from Korean traditional food, Jeotgal. The isolate was identified as Bacillus megaterium KS12 by examining its biochemical properties and 16S rRNA sequence analysis (99% similarity). B. megaterium KS12 exhibited high viability in artificial gastric juice for 3 h and in artificial bile salts for 24 h, about 14.4 and 14%, respectively. The herbal digestive medicine containing the isolate showed high starch hydrolytic activity and proteolytic activities (about 6-fold) compared to traditional herbal digestive medicine. The viable cells in the herbal digestive medicine containing the isolate were higher than those of traditional herbal digestive medicine.

• Asian-Australasian Journal of Animal Sciences
• /
• 제18권3호
• /
• pp.409-413
• /
• 2005

The objective of this research was to combine the physiological functionality of probiotics (Lactobacillus acidophilus and Bifidobacterium longum) and the milk-clotting activity of culture filtrate from lao-chao to develop a new dairy product which was different from the commercial yogurt. Rhizopus javanicus and Saccharomyces cerevisiae were chosen as a mold and yeast starter for production of culture filtrate. The study results indicated that both probiotic counts increased with incubation time and maintained $10^7$-$10^8$ CFU/ml after 6 h incubation with 10-30% culture filtrates. By contrast, samples with 40% culture filtrates inhibited the growth of L. acidophilus and B. longum. The more culture filtrates were added, the lower titratable acidities and higher pH values in Kou Woan Lao were detected after 36 h fermentation. No significant differences (p>0.05) were found for both L. acidophilus and B. longum, when grown in differing concentrations of skim milk powders. Storage results showed both L. acidophilus and B. longum exhibited excellent stability for 14 days at $4^{\circ}C$ in the Kou Woan Lao.

• Journal of Microbiology and Biotechnology
• /
• 제30권5호
• /
• pp.726-732
• /
• 2020

Indonesian kefir grains are potential sources of lactic acid bacteria (LAB) that may act as probiotics with specific functional properties. In this study we explored the potential of the probiotic and cholesterol-lowering effect of LAB isolated from Indonesian kefir grains obtained from Bogor, Bandung, Jakarta, and Yogyakarta. The results revealed that 10 isolates showed considerable survivability at low pH and bile salt with total cell reduction of ~3 log colony-forming units per milliliter after exposure to pH 2.5 and 0.5% (w/v) bile salt for 1 and 3 h, respectively. All strains exhibited strong antimicrobial activities against pathogenic bacteria and were sensitive to a wide spectrum of antibiotics but exhibited weak bile salt hydrolase activity. Identification based on 16S RNA suggested that nine isolates were Lactobacillus kefiri and one was Lactobacillus rhamnosus. The ability of the isolates to reduce cholesterol from the media varied, ranging from 22.08% to 68.75% with the highest reduction shown by L. kefiri JK17. The ability to remove cholesterol from the media decreased greatly in resting and dead cells, ranging from 14.58% to 22.08% in resting cells and from 7.89% to 18.17% in dead cells. It can be concluded that Indonesian kefir grains contain LAB potentially acting as probiotics capable of reducing cholesterol. The cholesterol-lowering effect especially occurs when the cells are metabolically active.

• 대한수의학회지
• /
• 제56권2호
• /
• pp.75-84
• /
• 2016

Probiotics that are able to provide beneficial effects on animal health have become important ingredients of dog foods. This study was conducted to characterize the probiotic potentials of two strains, Lactobacillus reuteri BCLR-42 and Lactobacillus plantarum BCLP-51, that were derived from feces of healthy dogs and evaluated based on tolerance to low pH and bile acid, antimicrobial activities, enzyme profiles, sensitivity to antibiotics, and innate immune enhancing potentials. Both strains showed survival of more than 90% at pH 3 and 0.2% bile acid and exhibited broad antimicrobial activities against indicator bacteria. Moreover, both strains showed high sensitivity to antibiotics, except vancomycin, metronidazole, and gentamicin. The alkaline phosphatase was negligible (score 0), whereas they showed strong beta galactosidase activity (score range 5 or 3, respectively). The phagocytosis and oxidative burst activities of canine granulocytes were significantly enhanced in response to both strains. These results show that both strains have the capability to act as probiotics and the potential for application as ingredients in dog foods.

• 한국축산식품학회지
• /
• 제40권6호
• /
• pp.1014-1032
• /
• 2020

This work aimed to investigate the psychobiotic effects of six bacterial strains on the mind and behavior of male Wistar rats. The probiotic (PRO) group (n=7) were rats pre-treated with antibiotics for 7 days followed by 14-day probiotic administration, antibiotics (ANT) group (n=7) were rats treated with antibiotics for 21 days without probiotics. The control (CON) group (n=7) were rats that received sham treatment for 21 days. The six bacterial strains with probiotic properties were mostly isolated from Thai fermented foods; Pedicoccus pentosaceus WS11, Lactobacillus plantarum SK321, L. fermentum SK324, L. brevis TRBC 3003, Bifidobacterium adolescentis TBRC 7154 and Lactococcus lactis subsp. lactis TBRC 375. The probiotics were freeze-dried into powder (6×10⁹ CFU/5 g) and administered to the PRO group via oral gavage. Behavioral tests were performed. The PRO group displayed significantly reduced anxiety level and increased locomotor function using a marble burying test and open field test, respectively and significantly improved short-term memory performance using a novel object recognition test. Antibiotics significantly reduced microbial counts in rat feces in the ANT group by 100 fold compared to the PRO group. Probiotics significantly enhanced antioxidant enzymatic and non-enzymatic defenses in rat brains as assessed using catalase activity and ferric reducing antioxidant power assay, respectively. Probiotics also showed neuroprotective effects with less pyknotic cells and lower frequency of vacuolization in cerebral cortex. This multi-strain probiotic formulation from Thai fermented foods may offer a potential to develop psychobiotic-rich functional foods to modulate human mind and behaviors.

• 한국미생물·생명공학회지
• /
• 제31권3호
• /
• pp.297-300
• /
• 2003

In order to select probiotics with a high survival rate in gut and the growth inhibition of virulent pathogens to human beings or animals, we have screened lactic acid bacteria and yeasts from Jeot-gal to assess resistance against the artificial gastric acid and bile juice. Lactic acid bacteria and yeasts isolated were incubated for 24 h in artificial bile juice after incubation for 2 h in artificial gastric acid. Especially, strain HW 161 and strain NK 181 showed the higher survival for 2 h incubation in artificial gastric acid. All of 3 strains of lactic acid bacteria and 2 strains of yeast were showed higher growth rate than the control in artificial bile. The antimicrobial activity of lactic acid bacteria and yeasts was also investigated to prove efficacy as probiotic organisms. Lactic acid bacteria were shown the inhibition of Gram positive and negative bacteria, but yeasts narrow inhibition.

• 한국식품영양과학회지
• /
• 제28권5호
• /
• pp.1003-1009
• /
• 1999

For the isolation of probiotics which may be used for the human beings and animals, we have screened the microorganisms from chicken intestines which have acid and bile tolerance and the growth inhibition of pathogenic E. coli and Salmonella typhimurium. Among them, a strain which was identified as Lactobacillus salivarius had around 66% of survival after 2h incubation in the artificial gastric juice and 9% of survival after 24h incubation in the presence of 0.3% bile salts, and showed complete inhibition against both path ogenic E. coli and Salmonella typhimurium after 24 h coincubation. Its storage stability after lyophilization could be improved by adding polyvinylpyrrolidone.

• Journal of Dairy Science and Biotechnology
• /
• 제37권1호
• /
• pp.40-48
• /
• 2019

Bifidobacteria are a prototype probiotic, which normally inhabit the intestinal tract of humans. In the present study, four species of Bifidobacterium isolated from the feces of infants were characterized. The tolerance for acid or bile salt, autoaggregation, and antibiotic resistance of the bacteria were examined. The four species were resistant to low pH, bile salts, and up to 3% bile acid. Autoaggregation rates were as high as 90%. The bacteria were consistently resistant to gentamicin, kanamycin, streptomycin, ciprofloxacin, and nalidixic acid. Due to their tolerance to environmental factors like acid and bile salts, B. longum MG723, B. breve MG729, B. bifidum MG731, and B. animalis subsp. lactis MG741 are potentially valuable as probiotics and may be useful for industrial application.

• 한국초지조사료학회지
• /
• 제26권4호
• /
• pp.293-300
• /
• 2006

본 시험은 제주도내 발생하는 돈분액비의 활용도를 높이고 화학비료 사용 절감 및 대처하는 목적으로 수행되어졌다. 품종간의 건물수량은 수수${\times}$수단그라스가 $1,480.4kg/10a$로 가장 높았으며, 수수${\times}$수수교잡종이 1,444.9.5 kg/10a, 옥수수 1,054.6 kg/10a로 나타났다.(p<0.01). 처리별 건물수량은 미생물제제를 처리한 돈분액비가 1,562 kg/10a로 높게 나타났으며, 돈분액비 시용구는 1,410 kg/10a, 화학비료구 1,008 kg/10a로 나타났다(p<0.01). 토양성분은 시험 전 1.32 ppm이었던 Zn 함량이 시험 후 화학비료구, 돈분액비구, 미생물제제를 처리한 돈분액비구에서는 4.23, 4.12, 4.00 ppm으로 나타났으며, Cu 함량은 시험 전 0.59 ppm 시험 후에 토양에서는 화학비료구, 돈분액비구, 미생물제제를 처리한 돈분액비구에서는 각각 0.821, 0.854, 1.027 ppm으로 높아졌다. 이 시험의 결과로 미생물제제를 처리한 돈분액비가 화학비료구나 일반돈분액비에 비해 비료성분이 떨어지지 않으며 관수효과까지 있어 사료작물에 적합한 비료자원이라 할 수 있겠다.