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Production and Characterization of Herbal Digestive Medicine Containing Probiotics  

Choi, Hak Joo (Traditional and Biomedical Research Center, Daejeon University)
Kim, Dong Hee (Traditional and Biomedical Research Center, Daejeon University)
Ji, Joong Gu (Department of Oriental Health Care, Joongbu University)
Miyamoto, Tomofumi (Department of Chemo-Pharmaceutical Science, Faculty of Pharmaceutical Sciences, Kyusu University)
Shin, Kwang Soo (Department of Microbiology & Biotechnology, College of Natural Sciences, Daejeon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.27, no.1, 2013 , pp. 107-111 More about this Journal
Abstract
To investigate the possible usage as probiotics, we isolated and identified one strain of bacillus from Korean traditional food, Jeotgal. The isolate was identified as Bacillus megaterium KS12 by examining its biochemical properties and 16S rRNA sequence analysis (99% similarity). B. megaterium KS12 exhibited high viability in artificial gastric juice for 3 h and in artificial bile salts for 24 h, about 14.4 and 14%, respectively. The herbal digestive medicine containing the isolate showed high starch hydrolytic activity and proteolytic activities (about 6-fold) compared to traditional herbal digestive medicine. The viable cells in the herbal digestive medicine containing the isolate were higher than those of traditional herbal digestive medicine.
Keywords
herbal digestive medicine; B. megaterium KS12; probiotics;
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