• Title/Summary/Keyword: probing

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Evaluation of periopathogenic bacteria of peri-implantitis using real-time PCR in Koreans: pilot study (한국인의 임플란트 주위 질환에서 real time PCR법을 이용한 치주세균 평가: pilot study)

  • Lee, Mun-Young;Kwon, Eun-Young;Kim, Hyun-Joo;Lee, Ju-Youn;Joo, Ji-Young
    • Journal of Dental Rehabilitation and Applied Science
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    • v.34 no.3
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    • pp.186-195
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    • 2018
  • Purpose: We performed quantitative and qualitative analysis of typical periodontal bacteria using real time PCR method to investigate the microbiological difference according to the severity of peri-implant disease in Koreans. Materials and Methods: Total of 60 implants were divided into three groups (healthy group, peri-implant mucositis group, peri-implantitis group) through periapical radiographs and clinical indices. The evaluated clinical parameters were pocket depth, plaque index, suppuration and bleeding on probing. Using a sterilized curette instrument, microbial samples were collected from the subgingival plaque and real-time PCR was performed on five periodontal bacteria. The relative expression levels of microorganisms were compared by comparative delta-CT method. Results: The relative expression levels of E. corrodens and T. denticola were significantly higher in the peri-implantitis group (P < 0.017). On the other hand, the relative expression level of F. nucleatum and P. gingivalis was relatively high in the healthy implant group regardless of the severity of disease. P. intermedia was significantly lower in the healthy implant group (P < 0.017). Conclusion: Periodontal bacteria were detected in Koreans with peri-implant diseases, but there was no microbiological distribution similar to periodontitis.

The effect of nutraceutical containing PRF-K2 on periodontal condition during maintenance phase (치주 유지관리기 환자에게 PRF-K2를 함유한 nutraceutical의 효과)

  • Kim, Yu-Kang;Chung, Hyun-Ju;Kim, Se-Won;Baek, Dong-Heon
    • Journal of Periodontal and Implant Science
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    • v.37 no.1
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    • pp.91-102
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    • 2007
  • The long term success of periodontal treatment is dependent upon the effectiveness of the main-tenance care program after active treatment. The purpose of this study was to evaluate whether nutraceutical containing PRF-K2 as natural product from plant and seaweed has beneficial effects on clinical parameters, gingival crevicular fluid (GCF) volume and GCF cytokine levels during main- tenance phase after periodontal treatment. Among the generally healthy and non-smoking. moderate to severe chronic periodontitis patients during maintenance phase in Department of Periodontics, Chonnam National University Hospital, twenty eight patients took nutraceutical containing PRF-K2 (Oscotec Inc. Cheonan, Korea) for 3 months as experimental group and sixteen patients received only maintenance care as control group. Clinical examination and GCF collection were performed at baseline, 1, 2 and 3 months of experiment. Total amounts and concentrations of GCF IL-1{\beta}, IL-1ra and $PGE_2$ were evaluated using ELISA kit. In probing pocket depth, experimental group showed the tendency of more reduction than control group after 3 months of experiment. Sulcus bleeding index (SBI) and GCF volume were significantly decreased in experimental group(p<0.05), whereas they were increased in control group. GCF IL-1{\beta} level tended to decrease in both experimental and control group and IL-1ra concentration tended to increase in experimental group and to decrease in control group. IL-1ra/IL-1{\beta} ratio tended to increase in experimental group and to decrease in control group during experimental period. GCF $PGE_2$ amount did not show any change in experimental group and tended to increase in control group. These results suggest that nutraceutical supplement which contain PRF-K2 could improve perio-dontal condition during maintenance phase after periodontal therapy.

Effect of scaling and root planing on the expression of anti-inflammatory cytokines (IL-4, IL-9, IL-10, and IL-13) in the gingival crevicular fluid of electronic cigarette users and non-smokers with moderate chronic periodontitis

  • Al-Hamoudi, Nawwaf;Alsahhaf, Abdulaziz;Deeb, Modhi Al;Alrabiah, Mohammed;Vohra, Fahim;Abduljabbar, Tariq
    • Journal of Periodontal and Implant Science
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    • v.50 no.2
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    • pp.74-82
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    • 2020
  • Purpose: The aim of this cross-sectional study was to investigate the effect of scaling and root planing (SRP) on the expression of anti-inflammatory cytokines (interleukin [IL]-4, IL-9, IL-10, and IL-13) in the gingival crevicular fluid (GCF) of electronic cigarette users and non-smokers with moderate chronic periodontitis (CP). Methods: Electronic cigarette users and non-smokers with CP were included in the study. Full-mouth plaque and gingival indices, probing depth (PD), clinical attachment loss (CAL), and marginal bone loss (MBL) were assessed. The GCF was collected, and its volume and levels of IL-4, IL-9, IL-10, and IL-13 were assessed. These parameters were evaluated at baseline and 3 months after SRP. The sample size was estimated, and comparisons between groups were performed. P<0.05 was considered to indicate statistical significance. Results: Thirty-six electronic cigarette users (47.7±5.8 years old) and 35 non-smokers (46.5±3.4 years old) with CP were included. At baseline, there were no differences in plaque index (PI), PD, CAL, MBL, and GCF IL-4, IL-9, IL-10, and IL-13 between electronic cigarette users and nonsmokers. At the 3-month follow-up, there were no significant differences in PI, gingival index (GI), PD, CAL, and MBL in electronic cigarette users compared to baseline, while there were significant reductions in PI, GI, and PD among non-smokers. At the 3-month follow-up, GCF IL-4, IL-9, IL-10, and IL-13 levels were significantly elevated in both groups (P<0.05) compared to baseline. The increases in GCF IL-4, IL-9, IL-10, and IL-13 levels were significantly higher in non-smokers (P<0.05) than in electronic cigarette users at the 3-month follow-up. Conclusions: Levels of GCF IL-4, IL-9, IL-10, and IL-13 increased after SRP in electronic cigarette users and non-smokers with CP; however, the anti-inflammatory effect of SRP was more profound in non-smokers than in electronic cigarette users.

Platelet-rich fibrin along with a modified minimally invasive surgical technique for the treatment of intrabony defects: a randomized clinical trial

  • Ahmad, Nabila;Tewari, Shikha;Narula, Satish Chander;Sharma, Rajinder Kumar;Tanwar, Nishi
    • Journal of Periodontal and Implant Science
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    • v.49 no.6
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    • pp.355-365
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    • 2019
  • Purpose: The modified minimally invasive surgical technique (M-MIST) has been successfully employed to achieve periodontal regeneration. Platelet-rich fibrin (PRF) is known to enhance wound healing through the release of growth factors. This study aimed to observe the outcomes of periodontal surgery when M-MIST was used with or without PRF for the treatment of isolated intrabony defects. Methods: This randomized clinical trial was conducted on 36 systemically healthy patients, who had chronic periodontitis associated with a single-site buccal probing pocket depth (PPD) and clinical attachment level of ≥5 mm. Patients were randomly divided into 2 groups: the test group treated with M-MIST and PRF, and the control group treated with M-MIST alone. The primary periodontal parameters analyzed were PPD, relative attachment level (RAL), and relative gingival margin level. The radiographic parameters analyzed were change in alveolar crest position (C-ACP), linear bone growth (LBG), and percentage bone fill (%BF). Patients were followed up to 6 months post-surgery. Results: Intragroup comparisons at 3 and 6 months showed consistently significant improvements in PPD and RAL in both the groups. In intergroup comparisons, the improvement in PPD reduction, gain in RAL, and the level of the gingival margin was similar in both groups at 3 and 6 months of follow-up. Furthermore, an intergroup comparison of radiographic parameters also demonstrated similar improvements in C-ACP, LBG, and %BF at 6 months of follow-up. Conclusions: M-MIST with or without PRF yielded comparable periodontal tissue healing in terms of improvements in periodontal and radiographic parameters. Further investigation is required to confirm the beneficial effects of PRF with M-MIST.

COMPARATIVE STUDY ON THE EFFICACY OF DIGITAL IMAGING FIBER-OPTIC TRANS-ILLUMINATION AND LASER FLUORESCENCE IN MONITORING THE REMINERALIZATION PROCESS OF INCIPIENT SMOOTH SURFACE ENAMEL LESIONS (Digital Imaging Fiber-Optic Trans-Illumination과 Laser Fluorescence를 이용한 평활면 초기우식증의 재광화에 관한 비교 연구)

  • Hwang, Kyu-Seon;Yoo, Seung-Hoon;Kim, Jong-Soo
    • Journal of the korean academy of Pediatric Dentistry
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    • v.34 no.2
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    • pp.183-191
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    • 2007
  • Through out the world dental caries seems to be decreased as it is difficult to make an accurate diagnosis for dental caries. The traditional diagnostic method which is probing and x-ray taking has many limitations to diagnose the early caries, so there were recommendations for the needs of new equipments such as laser fluorescence(LF), digital imaging fiber-optic trans-illumination(DIFOTI), and quantitative light fluorescence (QLF) which were developed from various study results. Also confocal laser scanning microscopy(CLSM) and ultrasonics are used for research progression. This study is to evaluate whether it is possible to monitor accurately for remineralization amount of enamel surface early caries using DIFOTI or LF After inducing artificial caries to bovine teeth to 10 participants remineralization was enhanced by 0 ppm and 500 ppm fluoride mouth rinse solution for 3 weeks. Then they were cross sectioned and analyzed using gold standard of the lesion depth measured by CLSM. The following results were obtained: 1. The measured percentage of light intensity(luminosity ratio) by DIFOTI increased with remineralization period, and showed significant reverse correlation with lesion depth measured by CLSM (p<0.01). 2. The measurement of laser fluorescence increased with remineralization period, and showed significant correlation with lesion depth measured by CLSM (p<0.01). 3. To the result for CLSM, 500 ppm fluoride mouth rinse group showed rapid rate for decreased tendency of lesion depth than 0 ppm fluoride mouth rinse group. In conclusion DIFOTI system was used to measure accurately for the remineralization amount of early surface caries, it is a very useful equipment to detect precisely the changes for early enamel caries remineralization during treatments.

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A Study of Brand Value Development on Convention Destination: Application of Means-End Chain and Laddering Technique (Means-End Chain과 Laddering을 이용한 컨벤션도시의 브랜드가치 개발에 관한 연구)

  • Oh, Chang-Ho;Yook, Poong-Rim;Hwang, Jae-Wie;Kang, Sun-Goo
    • Management & Information Systems Review
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    • v.31 no.2
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    • pp.253-272
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    • 2012
  • One of the fastest growing sectors in tourism industry is the use of applications from the MICE industry in various parts including business, politics, and cultural exchange due to globalization among the countries. The means-end chain theory suggests that convention participants perceive and judge convention destination as the means to achieving a desired end-state in a given destination selection situation. This study aims at establishing a convention destination's attributes, the benefits of consequences of using it, and the personal values it satisfies. A laddering interview was conducting in order to provide in-depth probing and to elicit responses from 96 visitors. This study presents the unique pyramid-structure of the means-end chain; a model linking perceived convention destination attributes to values. Understanding these concepts provides opportunities for convention destinations to target market segmentation and advertising based on the participants' desired end-states. In conclusion, the major correlations of attributions of the visitors, consequences, and the values are; informativeness(A), activity(A), tourist attraction(A) - sympathy(C), human relations(C) - self-development(V), and fellowship(V).

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Effects of the Short-Term Weight Control Program on Periodontal Health in the University Students: A Pilot Study (일개 대학생의 단기 체중조절 프로그램이 치주건강에 미치는 영향: 사전연구)

  • Koong, Hwa-Soo;Son, Soo-Jung;Park, Hoo-Seob;Seo, Hyung-Seok;Hwang, Soo-Jeong
    • Journal of dental hygiene science
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    • v.15 no.4
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    • pp.413-418
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    • 2015
  • Recent cross-sectional studies indicate that obesity is a risk factor for periodontal disease. This study was aimed to investigate whether the four-week weight control program including caloric restriction and exercise training could have an effect on periodontal health. Forty-one obese (body mass index [BMI] ${\geq}25.0$) and five overweight ($23.0{\leq}BMI<25.0$) students participated in the weight control program. Anthropometric data and oral examination data were collected at the baseline and at the 27th day. BMI, waist hip ratio (WHR), and percent of body fat (PBF) of the subjects decreased significantly, but gingival index, sites with bleeding on probing (BOP), and sites with shallow pocket depth didn't show the significant changes in paired t-test. There was no difference in the outcomes according to smoking, drinking alcohol, and sex. Nevertheless, PBF and sites with BOP (r=0.777) and WHR and sites with shallow pocket depth (r=0.444) showed positive correlations. PBF accounted for 58.9% of the variance in sites of BOP in regression analysis. We suggested that obesity might relate with periodontal health, although it was not clear whether weight control could influence on periodontal health directly.

Relationship of soil profile strength and apparent soil electrical conductivity to crop yield (실시간 포장에서 측정한 토양 경도 및 전자장 유도 전기전도도와 작물수량과의 관계)

  • Jung, Won-Kyo;Kitchen, Newell R.;Sudduth, Kenneth A.
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.2
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    • pp.109-115
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    • 2006
  • Understanding characteristics of claypan soils has long been an issue for researchers and farmers because the high-clay subsoil has a pronounced effect on grain crop productivity. The claypan restricts water infiltration and storage within the crop root zone, but these effects are not uniform within fields. Conventional techniques of identifying claypan soil characteristics require manual probing and analysis which can be quite expensive; an expense most farmers are unwilling to pay. On the other hand, farmers would be very interested if this information could be obtained with easy-to-use field sensors. Two examples of sensors that show promise for helping in claypan soil characterization are soil profile strength sensing and bulk soil apparent electrical conductivity (ECa). Little has been reported on claypan soils relating the combined information from these two sensors with grain crop yield. The objective of this research was to identify the relationships of sensed profile soil strength and soil EC with nine years of crop yield (maize and soybean) from a claypan soil field in central Missouri. A multiple-probe (five probes on 19-cm spacing) cone penetrometer was used to measure soil strength and an electromagnetic induction sensor was used to measure soil EC at 55 grid site locations within a 4-ha research field. Crop yields were obtained using a combine equipped with a yield monitoring system. Soil strength at the 15 to 45 cm soil depth were significantly correlated to crop yield and ECa. Estimated crop yields from apparent electrical conductivity and soil strength were validated with an independent data set. Using measurements from these two sensors, standard error rates for estimating yield ranged from 9 to 16%. In conclusion, these results showed that the sensed profile soil strength and soil EC could be used as a measure of the soil productivity for grain crop production.

A Tooth Morphologic Study Of The Mandibular Molars With Furcation Involvement (치근이개부병소를 지닌 하악대구치에 대한 치아형태학적연구)

  • Jin, Hyung-Kook;Kim, Hyun-Seop;Kim, Byung-Ok;Han, Kyung-Yoon
    • Journal of Periodontal and Implant Science
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    • v.26 no.1
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    • pp.266-275
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    • 1996
  • The purpose of this study was to evaluate the effect of anatomical predisposing factors on the development of furcation involvement. Root trunk length, root divergency angle, buccal root trunk concavity and cervical enamel projection of the mandibular 1st and 2nd molars with(l03 teeth) and without(42 teeth) furcation involvement and enamel projection were classified by Lindhe's degree and Masters's classification, respectively, and buccal root trunk concavity was examined by probing. Statistical analysis was performed by means of ANOVA and CHI-SQUARE test in Microstat. The obtained results were as follows : 1. Root trunk length was longer in teeth without furcation involvement($4.20{\pm}1.05mm$) than teeth with furcation involvement(I : $3.62{\pm}0.68mm$, II : $3.64{\pm}0.86mm$, III: $3.61{\pm}0.74mm$)(p<0.05), but there was fno significant difference among furcation involvement group according to the degree of furcation involvement(p0>.05). 2. The root divergency angle was wider in teeth with furcation involvement(I : $53.14^0{\pm}15.11^0$, II : $44.82^0{\pm}14.26^0$, III : $52.69^0{\pm}16.09^0$) than teeth without furcation involvement($34.81^0{\pm}16.57^0$(p<0.05). 3. The group of teeth without furcation involvement showed significantly hign percentage of teeth without buccal root concavity, and the group of teeth with furcation involvement showed significantly hign percentage of teeth with buccal root concavity(p<0.05) 4. The group of teeth without furcation involvement showed higher percentage of teeth with grade I cervical enamel projection, teeth with furcation involvement I or II defect showed higher percentage of teeth with grade II enamel projection, and teeth with furcation involvement III defect showed higher percentage of teeth with grade III enamel projection(p<0.05) The results suggest that short root trunk length, wide root divergency, buccal root concavity and well-developed enamel projection could affect development and progression of furcation involvement as anatomical predisposing factors of periodontal diseases.

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NOVEL TREATMENT MODALITY OF IMPLANT-RETAINED OVERDENTURE FOR IMMEDIATE FUNCTION IN COMPLETELY EDENTULOUS PATIENTS (무치악 환자에서 하악 임플랜트 지지 피개의치에 의한 즉시 하중 부담에 관한 연구)

  • Kim, Jang-Wook;Kim, Hyeong-Seob;Baik, Jin;Kwon, Kung-Rock
    • The Journal of Korean Academy of Prosthodontics
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    • v.45 no.1
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    • pp.34-47
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    • 2007
  • Statement of problem: Conventional mandibular complete denture lacks in support and stability for edentulous patients to use. Purpose: To enhance support and stability of mandibular complete denture, 2 implants were inserted on the inter-mental foramina space of edentulous patients. With 2 magnetic attachments on the inner side of the overdenture, we gave immediate loads and evaluated the stability of the implants, the responses of the supporting tissues, and the satisfaction of the patients. Material & methods: 6 edentulous patients (5 male, 1 female) each recieved 2 SLA implants in the inter-mental foramina region and implants were immediately loaded with overdenture with magnet attachments. To evaluate the implants stability and the peri-implant tissues, clinical exams, RFA tests and radiographic exams were preformed at the 1 week, 2wks, 6wks, 12wks, and 24wks. post- surgery. Results: The mean surgery time was $45.7{\pm}7.7mins$., while the denture delivery time was $45.5{\pm}12.6mins$. Only 2 of 14 implants were failed. Survived implants that remained were clincally and radiographically stable. Mean ISQ values were relatively stable, showing $69.71{\pm}5.55$, $69.00{\pm}9.48$, $67.92{\pm}7.86$, $67.92{\pm}9.58$, $70.08{\pm}7.61$, $71.92{\pm}6.43$ at the 1 wk, 2 wks, 6 wks, 12 wks, 24 wks. follow up check. Crestal bone changes were $-1.18mm{\pm}0.68mm$, $-1.35{\pm}0.69mm$, $-1.47{\pm}0.68mm$ at the 6wks, 12wks, 24wks. follow up check. Bleeding on probing(BOP index) was not significant. Conclusion: Mandibular ovedentures with 2 magnetic attachments over two interforaminal implants on edentulous patients for immediate function is a recommendable novel treatment for edentulous patients which shows stability on the implants and supporting tissue.