• Microbiology and Biotechnology Letters
• /
• v.18 no.4
• /
• pp.401-405
• /
• 1990

Maximum specific pro-UK production rate was achieved at 15 mllmin of perfusion rate as $5.7 \times 10^{-8}$ $\mu g$/h/cell by cytostatic cultivation of human cell line 579 with DMEM and 590 FBS. As perfusion rates were increased, glutamine uptake rates were also increased but ammonium production rates remained relatively constant, which resulted in low ratio of ammonium to glutamine at high perfusion rate. Partially it quantitatively explains why the productivity is increased in perfusion cultivations. At maintenance period of 15 days by controlling metabolic process, such as 5 mM of glucose, 2 mM of gtutamine, 10% of air saturation and pH 6.2, high speecific product production rate and product yield on substrate were obtained as $12\times 10^{-8}$$\mu g$/h/cell abd 0.226 mglg of glucose, respetively. This product yield corresponds to 0.223 mg/day of productivity at 10 mllmin of perfusion rate.

• Journal of Bio-Environment Control
• /
• v.19 no.4
• /
• pp.387-391
• /
• 2010

This research was conducted to get the basic information for improving production amount as well as fruit quality by using Prohaxadion-Calcium (Pro-Ca) used as 'Sato Nishiki', cherry cultivar, which is most frequently cultivated in Korea. In fruiting trees, we used Pro-ca and concentration of treatment selected 150, 200 and $250\;mg{\cdot}L^{-1}$. When treating Pro-Ca $200\;mg{\cdot}L^{-1}$, we could get the highest effect on inhibition of shoot growth. All treatments of Pro-Ca were increased chlorophyll a and b, respectively compared with control. Although treatments of Pro-Ca treatment showed the decreased effect on leaf area and there was no difference in fruit quality among treatments. Also, Pro-Ca $250\;mg{\cdot}L^{-1}$, sprayed 3 times, and pclobutrazol ($PP_{333}$) 500 and $1,000\;mg{\cdot}L^{-1}$, applicated soil, were treated to 1 year old plnted pot. Pro-Ca and $PP_{333}$ showed inhibition of shoot growth, especially, Pro-Ca showed the more effect than $pp_{333}$ on growth inhibition. Soil applications of $pp_{333}$ showed less effect to enlargement of tree trunk compared to Pro-Ca and control. Pro-Ca and $pp_{333}$ were not difference in number of inter-nodes when comparing to control, but they on decreased leaf area and increased chlorophyll.

• Journal of Korean Ophthalmic Optics Society
• /
• v.14 no.1
• /
• pp.69-80
• /
• 2009

Purpose: We investigated validity of a monitor-based computer eye test program, Test Chart 2000 Pro (developed by Thomson Software Solutions, UK). Methods: We chose ten common eye tests of the Test Chart 2000 Pro and Korean Standard Eye Test, applying them to same subject groups each by each, followed by comparison and analyses of agreement degree of the results. Results: Among the ten eye tests, Snellen Chart, Cross-cyl target, Duochrome test, Fan and Block test, and Random dot stereograms showed statistically significant agreement between both the Korean standard eye test method and Test Chart 2000 Pro. On the other hand, some disagreements were found between the two eye test methods in LogMAR Chart, Single Letter Chart, Phoria Test, Fixation Disparity Test, and Worth 4 Dot Test. Conclusions: Comparing to the Korean Standard Eye Test that consists of Han eye chart and Phoroptor, validity of the Test Chart 2000 Pro is not so high. Further improvements of the Test Chart 2000 Pro in accuracy are required.

• Journal of Animal Science and Technology
• /
• v.63 no.5
• /
• pp.1159-1168
• /
• 2021

Ovotransferrin (OTF), an egg protein known as transferrin family protein, possess strong antimicrobial and antioxidant activity. This is because OTF has two iron binding sites, so it has a strong metal chelating ability. The present study aimed to evaluate the improved immune-enhancing activities of OTF hydrolysates produced using bromelain, pancreatin, and papain. The effects of OTF hydrolysates on the production and secretion of pro-inflammatory mediators in RAW 264.7 macrophages were confirmed. The production of nitric oxide (NO) was evaluated using Griess reagent and the expression of inducible nitric oxide synthase (iNOS) were evaluated using quantitative real-time polymerase chain reaction (PCR). And the production of pro-inflammatory cytokines (tumor necrosis factor [TNF]-α and interleukin [IL]-6) and the phagocytic activity of macrophages were evaluated using an ELISA assay and neutral red uptake assay, respectively. All OTF hydrolysates enhanced NO production by increasing iNOS mRNA expression. Treating RAW 264.7 macrophages with OTF hydrolysates increased the production of pro-inflammatory cytokines and the phagocytic activity. The production of NO and pro-inflammatory cytokines induced by OTF hydrolysates was inhibited by the addition of specific mitogen-activated protein kinase (MAPK) inhibitors. In conclusion, results indicated that all OTF hydrolysates activated RAW 264.7 macrophages by activating MAPK signaling pathway.

• The Journal of Information Systems
• /
• v.19 no.4
• /
• pp.25-49
• /
• 2010

As companies introduced ERP (Enterprise Resource Planning) systems since the middle of 1990s, the databases of the companies has become centralized and gigantic. The companies are now developing data-mining based applications on those centralized and gigantic databases for knowledge management. Almost of them are using $Pro^*C$/C++, a embedded SQL programming language, and it's because the $Pro^*C$/C++ is independent of platforms and also fast. However, they suffer from difficulties in development and maintenance due to the characteristics of corporate databases which have intrinsically large number of tables and fields. The purpose of this research is to design and implement a methodology which makes it easier to develop and maintain embedded SQL applications based on relational databases. Firstly, this article analyzes the syntax of $Pro^*C$/C++ and addresses the concept of repetition and duplication which causes the difficulties in development and maintenance of corporate information systems. Then, this article suggests a management architecture of source codes and databases in which a preprocessor generates $Pro^*C$/C++ source codes by referring a DB table specification, which would solve the problem of repetition and duplication. Moreover, this article also suggests another architecture of DB administration in which the preprocessor generates DB administration commands by referring the same table specification, which would solve the problem of repetition and duplication again. The preprocessor, named $PrePro^*C$, has been developed under the UNIX command-line prompt environment to preprocess $Pro^*C$/C++ source codes and SQL administration commands, and is under update to be used in another DB interface environment like ODBC and JDBC, too.

• Journal of the Korea Convergence Society
• /
• v.10 no.1
• /
• pp.245-256
• /
• 2019

The study is aimed at examining the influence of empathy and pro-social behavior on school life happiness of multicultural and Korean adolescents through sports activities. The results of the analysis of total 461 adolescents participating in sports activities and not participating in sports activities are as follows. Adolescents participating in sports activities have higher levels of empathy, pro-social behavior and school life happiness than those who do not participate. And the more experience involved in sports activities, the higher the level of empathy, the level of pro-social behavior and the level of school life happiness. There is a significant correlation between empathy and pro-social behavior and school life happiness. Empathy and pro-social behavior factors affecting school life happiness are statistically influencing factors such as "relationship formation", "communication", "cooperation", "assistance", "emotional expression", and "comfort". The results of this study suggest the importance of dealing with empathy and pro-social behavior through sports activities in the school life happiness of multicultural families and Korean adolescents.

• Restorative Dentistry and Endodontics
• /
• v.31 no.1
• /
• pp.30-35
• /
• 2006

To evaluate the change of working length with various instrumentation techniques in curved canals, working length and canal curvature were determined before and after canal instrumentation in buccal or mesial canals of extracted human molars. Stainless steel K-files ($MANI^{(R)}$, Matsutani Seisakusho Co. Takanezawa, Japan), nickel-titanium K-files (Naviflex $NT^{TM}$, Brassier, Savannah, USA) , $ProFile^{(R)}$, and ProTaper (Dentsply-Maillefer, Ballaigues, Switzerland) were used to prepare the canals with crown-down technique. In two hand instrumentation groups coronal flaring was made with Gates Glidden burs. Apical canals were instrumented until apical diameter had attained a size of 30. Positional relation between the tooth apex and the $\#10$ K-file tip was examined by using AutoCAD 2000 (Autodesk Corp., San Rafael. CA, USA) under a stereomicroscope before and after coronal flaring, and after apical instrumentation. Degree of canal curvature was also measured with Schneider's method in radiographs. Data of working length and canal curvature changes were statistically analyzed with one-way ANOVA and Tukey's studentized range test. Working length and canal curvature were decreased significantly in each step in all instrumentation groups. Coronal flaring using Cates Glidden burs in hand instrument groups and whole canal instrumentation using stainless steel hand K-files caused significantly more working length change than in ProFile instrumentation group (p<0.05). The result of this study demonstrates that all of the above kinds of instrumentation in curved canals cause reduction of working length and canal curvature at each instrumentation steps, and hand instrumentation causes more working length change than ProFile.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
• /
• 2004.05a
• /
• pp.322-325
• /
• 2004

Kefir에서 유산균인 Lactobacillus acidophilus와 Lactococcus lactis 그리고 효모인 Candida kefir를 분리하였고, 이를 이용하여 FM(Fermented milk) A, B, C 제조${\cdot}$분석하였다. 유산균 수는 FM B가 $8.6{\times}\;10^9\;cfu/ml$로 가장 많았고, 효모수는 FM C가 $1.3{\times}\;10^7cfu/ml$로 가장 많았다. 알콜 함량은 FM C가 3.2%, FM A가 0.98%, FM B가 0.15% 였다. 아미노산은 FM A에서는 Glu, Pro, Leu, Lys, Asp 등이, FM B에서는 Glu, Leu, Pro, Lys 등이, FM C에서는 Glu, Leu, Pro, Lys 등이 주요 아미노산으로 나타났다. 지방산은 FM A, FM B, FM C 모두 palmitic acid(Cl6:0)와 oleic acid(Cl8:1)가 각각 33.5${\sim}$37.7%와 22.1${\sim}$22.4%의 수준으로 주요 지방산을 차지하였다. 관능검사 결과FM C가 가장 높은 점수를 받았으며, 다음은 FM A, FM B 순이었다.

• Journal of The Korean Association For Science Education
• /
• v.38 no.5
• /
• pp.681-691
• /
• 2018

The purpose of this study is to investigate the impacts of Collaborative Problem solving for Character competency (CoProC) strategy on the practical character competency and collaborative problem solving competency in middle school. For this study, 49 seventh grade students (two classes) were selected for use applying the CoProC strategy (CoProC group) while 46 students (two classes) were used for traditional lecture and experimental class (comparative group). In the result, CoProC group showed a statistically significant result in practical character competency than the comparative group. In the sub items of the competency, the CoProC group showed result statistically significant in cooperation, communication, responsibility, and positive self-understanding than the comparative group. Analysis of the effect size of students' practical character competency showed that the CoProC group results showed more effective than the comparative group in terms of care, cooperation, communication, honesty, responsibility, positive self-understanding, and self-regulation. In addition, we investigated the effect of the CoProC strategy on collaborative problem solving competency. As a result, it showed a large effect in the total score of collaborative problem solving competency. Among the sub items of the competency, 'exploration and understanding of members' showed a small size of effect and 'Establishing and maintaining team organization' showed a medium size of effect. 'Communication' and 'self-reflection' showed a large effect. CoProC strategy embedded in Science subject could improve students' collaborative problem solving competency through the process of scientific communication in the scientific inquiry process.

• The Journal of the Korean dental association
• /
• v.50 no.8
• /
• pp.507-515
• /
• 2012

The purpose of this article is to discuss the effect of aging on the treatment of replantation and transplantation of teeth in old patients. I case of replantation and 2 cases of transplantations in old patients are reported in this article. Patients aged 65 and over are included. They had several problems such as periapical lesion, crack or foot fractures. In those cases, the replantation and transplantation were treatment of choice. White ProRoot MTA was used in apical retrograde filling and perforation repair. After replantation and transplantation of teeth, follow-up visits showed signs of healing in 3 cases. Considerations and indications for replantation and transplantation are not much affected by age. Old patients are aware that replantation and transplantation can be performed comfortably and that age is not a factor in prediction success.