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http://dx.doi.org/10.5187/jast.2021.e82

Improved immune-enhancing activity of egg white protein ovotransferrin after enzyme hydrolysis  

Lee, Jae Hoon (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Kim, Hyeon Joong (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Ahn, Dong Uk (Department of Animal Science, Iowa State University)
Paik, Hyun-Dong (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Publication Information
Journal of Animal Science and Technology / v.63, no.5, 2021 , pp. 1159-1168 More about this Journal
Abstract
Ovotransferrin (OTF), an egg protein known as transferrin family protein, possess strong antimicrobial and antioxidant activity. This is because OTF has two iron binding sites, so it has a strong metal chelating ability. The present study aimed to evaluate the improved immune-enhancing activities of OTF hydrolysates produced using bromelain, pancreatin, and papain. The effects of OTF hydrolysates on the production and secretion of pro-inflammatory mediators in RAW 264.7 macrophages were confirmed. The production of nitric oxide (NO) was evaluated using Griess reagent and the expression of inducible nitric oxide synthase (iNOS) were evaluated using quantitative real-time polymerase chain reaction (PCR). And the production of pro-inflammatory cytokines (tumor necrosis factor [TNF]-α and interleukin [IL]-6) and the phagocytic activity of macrophages were evaluated using an ELISA assay and neutral red uptake assay, respectively. All OTF hydrolysates enhanced NO production by increasing iNOS mRNA expression. Treating RAW 264.7 macrophages with OTF hydrolysates increased the production of pro-inflammatory cytokines and the phagocytic activity. The production of NO and pro-inflammatory cytokines induced by OTF hydrolysates was inhibited by the addition of specific mitogen-activated protein kinase (MAPK) inhibitors. In conclusion, results indicated that all OTF hydrolysates activated RAW 264.7 macrophages by activating MAPK signaling pathway.
Keywords
Ovotransferrin; Hydrolysates; Immune-enhancing activity; Mitogen-activated protein kinase (MAPK) pathway;
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