• Title/Summary/Keyword: prism

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Measurement and Analysis of the Dynamics of Peptide-Antibody Interactions Using an Ellipsometric Biosensor Based on a Silicon Substrate (실리콘 기판을 사용한 바이오센서와 회전 타원분광계를 이용한 펩타이드-항체 접합의 동특성 측정과 분석)

  • Lee, Geun-Jae;Cho, Hyun Mo;Jo, Jae Heung
    • Korean Journal of Optics and Photonics
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    • v.28 no.1
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    • pp.9-15
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    • 2017
  • We precisely measured and analyzed the dynamics of peptide-antibody interactions, using an ellipsometric biosensor based on a silicon substrate. To reduce the signal error due to the imperfect flatness of the substrate for extremely low concentrations of peptide, we fabricated the biosensor with a silicon substrate coated with Dextran SAM, instead of a glass prism coated with a thin metallic thin film. At an injection speed of $100{\mu}l/min$ of buffer liquid, we detected the dynamics of antibody-Dextran SAM or peptide-antibody fixed on biosensor, respectively. We detected the dynamics of antibody-Dextran SAM interactions down to a low concentration of 5 ng per liter, and we precisely measured the dynamics of association and dissociation of peptide and antibody down to 100 nM of peptide. We obtained the rate constants for association and dissociation from fitting the data by using deduced dynamical equation. As a result, we obtained an equilibrium constant for dissociation of 97 nM of peptide-antibody complex, which belongs to Class I.

Estimation of Critical Chloride Content for Corrosion of Reinforcing Steel in Concrete by Field Exposure Experiment (현장 폭로실험에 의한 콘크리트 중 철근의 부식 임계 염화물량 평가)

  • Yu, Kyung-Geun;Bae, Su-Ho;Park, Jae-Im;Lee, Kwang-Myong;Kim, Jee-Sang
    • Proceedings of the Korea Concrete Institute Conference
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    • 2008.04a
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    • pp.585-588
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    • 2008
  • To predict the service life of reinforced concrete structures exposed to chloride environment, quantitative measures of material properties such as the critical chloride content for corrosion in concrete and the diffusion coefficient of chloride ions of concrete and the surface chloride content of the concrete are essential. However, it should be noted that they are influenced by several factors such as concrete mix proportions, cement type, and environmental conditions, etc. Thus, the purpose of this research is to estimate more actually the critical chloride content for corrosion of the reinforcing steel in concrete by field exposure experiment. For this purpose, the prism concrete test specimens were made for water-cement(W/C) ratios of 31%, 42%, 50%, and 70%, and then the field exposure experiment for them were conducted at Youngduk of the east coast for about 3 years. During the test, corrosion monitoring by half cell potential method was carried out to detect the time to initiation of corrosion for test specimens and its chloride content was evaluated by breaking the concrete test specimens when corrosion of the reinforcing steel in concrete was perceived. It was observed from the test results that the critical chloride content for corrosion of reinforcing steel in concrete would be dependent on W/C ratio and almost irrespective of concrete cover.

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Slant Shear Test for Determining the Interfacial Shear Strength of Concrete Strengthened with Ultra-High Performance Fiber Reinforced Concrete (초고성능 섬유보강 콘크리트로 보강된 콘크리트의 계면 전단강도 결정을 위한 경사전단 실험)

  • Lim, Woo-Young;Hong, Sung-Gul
    • Journal of the Korea Concrete Institute
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    • v.28 no.6
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    • pp.637-646
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    • 2016
  • In this study, slant shear tests for the prism specimens strengthened with ultra-high performance fiber reinforced concrete (UHPFRC), normal- and high-strength concrete were performed to evaluate the interfacial shear strength between old and new concrete substrate. Test parameters are the roughness of surface, concrete strength, and fiber volume fraction of UHPFRC. The surface of the concrete was roughened by shot blasting. Test results showed that the adhesion bond resistance of the specimen with a roughened surface was very large compared to that of the specimen with a smooth surface. In addition, the interfacial shear strength appeared to be affected by the concrete strength rather than the fiber volume fraction. For the roughened surface by shot-blasting method, interfacial shear resistance exceeded the upper limit which is presented in current design codes even if the shear-friction reinforcements are not provided. Based on the test results, it is applicable to use the current concrete design codes to achieve the shear-friction design for the interface between conventional concrete and UHPFRC. However, for the surface which is not processed, it would be appropriate to provide additional shear-friction reinforcement.

Shear Strength of Ultra-High Performance Fiber-Reinforced Concrete(UHPFRC) I-shaped Beams without Stirrup (강섬유 보강 초고성능 콘크리트(UHPFRC) I형 보의 전단 강도)

  • Lee, Ji-Hyung;Hong, Sung-Gul
    • Journal of the Korea Concrete Institute
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    • v.29 no.1
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    • pp.53-64
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    • 2017
  • Ultra-high performance fiber-reinforced concrete (UHPFRC) is characterized by a post-cracking residual tensile strength with a large tensile strain as well as a high compressive strength. To determine a material tensile strength of UHPFRC, three-point loading test on notched prism and direct tensile test on doubly notched plate were compared and then the design tensile strength is decided. Shear tests on nine I-shaped beams with varied types of fiber volume ratio, shear span ratio and size effect were conducted to investigate shear behavior in web. From the test results, the stress redistribution ability represented as diagonal cracked zone was quantified by inclination of principal stress in web. The test results shows that the specimens were capable of resistance to shear loading without stirrup in a range of large deformation and the strength increase with post-cracking behavior is stable. However at the ultimate state all test specimens failed as a crack localization in the damaged zone and the shear strength of specimens is affected by shear span ratio and effective depth. Strength predictions show that the existing recommendations should be modified considering shear span ratio and effective depth as design parameters.

Single-crystal Structure of Fully Dehydrated and Largely NH4+-exchanged Zeolite Y (FAU, Si/Al = 1.70), │(NH4)60Na11│[Si121Al71O384]-FAU

  • Seo, Sung-Man;Kim, Ghyung-Hwa;Kim, Young-Hun;Wang, Lian-Zhou;Lu, Gao-Qing;Lim, Woo-Taik
    • Bulletin of the Korean Chemical Society
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    • v.30 no.3
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    • pp.543-550
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    • 2009
  • The single-crystal structure of largely ammonium-exchanged zeolite Y dehydrated at room temperature (293 K) and 1 ${\times}\;10^{-6}$ Torr. has been determined using synchrotron X-radiation in the cubic space group $Fd\overline{3}m\;(a=24.9639(2)\AA)$ at 294 K. The structure was refined to the final error index $R_1$ = 0.0429 with 926 reflections where $F_o>4\sigma(F_o)$; the composition (best integers) was identified as |$(NH_4)_{60}Na_{11}$|[$Si_{121}Al_{71}O_{384}$]-FAU. The 11 $Na^{+}$ ions per unit cell were found at three different crystallographic sites and 60 ${NH_4}^{+}$ ions were distributed over three sites. The 3 $Na^{+}$ ions were located at site I, the center of the hexagonal prism ($Na-O\;=\;2.842(5)\;\AA\;and\;O-Na-O\;=\;85.98(12)^{\circ}$). The 4 $Na^{+}$ and 22 ${NH_4}^{+}$ ions were found at site I' in the sodalite cavity opposite the double 6-rings, respectively ($Na-O\;=\;2.53(13)\;\AA,\;O-Na-O\;=\;99.9(7)^{\circ},\;N-O\;=\;2.762(11)\;\AA,\;and\;O-N-O =\;89.1(5)^{\circ}$). About 4 $Na^{+}$ ions occupied site II ($(Na-O\;=\;2.40(4)\;\AA\;and\;O-Na-O\;=\;108.9(3)^{\circ}$) and 29 ${NH_4}^{+}$ ions occupy site II ($N-O\;=\;2.824(9)\;\AA\;and\;O-N-O\;=\;87.3(3)^{\circ}$) opposite to the single 6-rings in the supercage. The remaining 9 ${NH_4}^{+}$ ions were distributed over site III' ($N-O\;=\;2.55(3),\;2.725(13)\;\AA\;and\;O-N-O\;=\;94.1(13),\;62.16(15),\;155.7(14)^{\circ}$).

Fabrication of the photon scanning tunneling microscope with constant intensity mode (일정광량 방식의 광자주사현미경 제작)

  • Kim, Ji-Taek;Choi, Wan-Hae;Jo, Jae-Heung;Chang, Soo;Kim, Dal-Hyun;Koo, Ja-Yong;Chung, Seung-Tae
    • Korean Journal of Optics and Photonics
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    • v.10 no.3
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    • pp.195-200
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    • 1999
  • We made sharp optical fiber tips with less than 100 nm diameter by using the heating and pulling method with a good repetition and fabricated the photon scanning tunneling microscope (PSTM) using constant intensity mode. The 3-dimensional PZT (Piezoelctric transducer) scanner made of a long PZT tube is consisted of three divided parts, that is, a pair of $\pm$ x and a pair of $\pm$y scanning parts and a z scanning part for the fine approach and scanning. The scanning dimension is 1.43 $\mu\textrm{m}$$\times$1.76 $\mu\textrm{m}$. The height of a optical tip to maintain a constant height within $1/{\lambda}_0$ (${\lambda}_0$ is the incident wavelength) from surface of a specimen to a optical tip is controlled automatically by using the electric feedback circuit. The 3-dimensional shape of standing evanescent waves generated on the surface of a dove prism was measured successfully by using the constant intensity mode PSTM.

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A Study on the Color Grouping System to Fashion (섬유컬러 그루핑 체계에 관한 연구)

  • 이재정;정재우
    • Archives of design research
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    • v.17 no.3
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    • pp.27-38
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    • 2004
  • It is important for designers to be supported with their decision-making on colours which is often based on personal distinction rather than logical dialogue that may lead to confusion within communicating with others. To help these problems and to gain productivity, we would like to propose a way to define colour grouping method. In other words, the purpose of this study is to help to improve the communication and productivity within the design and designers. The grouping was based and inspired by from the studies of Kobayashi, Hideaki Chijiawa, Allis Westgate and Martha Gill. The study of grouping is based on the "tones" of each group, as they seem to reflect a designer s sentimentalism of chosen colours the best. Each of these groups will be named Bright , Pastel ,Deep and Neutral The general concept of each groups are: - Bright: high quality of pixels of primary colour - Pastel: primary colour with white - - Deep: Primary colour with gray or black - Neutral: colours that does not include any of above Each of the colour group has been allocated into Si-Hwa Jung's colour charts and colour prism to visualize the relationships between the colour groups. These four groups and the colours included in them will be broken down to smaller groups in order to make colour palette. This would break the barrier and result in using colours in groups as well as crossover coordination. This study would result in new ways of using colurs for designers designers

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Detection of infectious canine hepatitis virus by TaqMan real-time PCR method (TaqMan 실시간 PCR법에 의한 개 전염성 간염 바이러스의 검출)

  • Wang, Hye-young;Choi, Jae-yong;Lee, Mi-jin;Park, Jin-ho;Cho, Mae-Rim;Han, Jae-cheol;Choi, Kyoung-seong;Chae, Joon-seok
    • Korean Journal of Veterinary Research
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    • v.44 no.4
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    • pp.655-662
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    • 2004
  • The aim of this work was the validation of a rapid real-time PCR assay based on TaqMan technology for the unequivocal identification of infectious canine hepatitis (ICH) virus, to be used directly on DNA purified from blood specimens. A real-time PCR system targeting at the E3 ORFA gene sequence of canine adenovirus type 1 was optimized and validated through comparative analysis of samples using conventional PCR system. The real-time PCR assay based on TaqMan technology could disclose 23 (37.7%) out of 61 samples as PCR positive. In contrast, 18 (29.5%) samples were found PCR positive when conventional PCR was applied on these samples. The use of the ABI Prism 7700 sequence detection system allowed the efficient determination of the amplified product accumulation through a fluorogenic probe. The entire real-time TaqMan PCR assay, including DNA extraction, amplification, and detection could be completed within 3 hours. The detection method of real-time TaqMan PCR assay was 1,000 times more sensitive than conventional PCR. Real-time TaqMan probe and primer set developed and optimized in this study is a sensitive, rapid and accurate method for detection of ICH virus and can be effective screening tool for the detection of ICH in a diagnostic laboratory routines.

Relationship Between Vergence Facility and Fusional Vergence (버전스용이와 융합버전스의 관계)

  • Shin, Hoy-Sun
    • Journal of Korean Ophthalmic Optics Society
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    • v.14 no.3
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    • pp.37-42
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    • 2009
  • Purpose: There is uncertainty if the vergence facility would provide clinically significant supplementary information to the fusional vergence measurements. The purpose of this study was to determine the relationship between vergence facility and fusional vergence in a group of symptomatic subjects. Methods: A total of 114 symptomatic subjects aged 9 to 13 years, who passed the vision screening test, participated in this study. Vergence facility was measured with 8$\Delta$ BI/8$\Delta$ BO flipper lenses and a suppression control target, the 20/30 letter line on Vectogram 9 (Bernell, USA). Near fusional vergence was measured with a single 20/30 vertical line target by Von Graefe technique. In order to avoid excessive convergence stimulation, negative fusional vergence (NFV) range (blur, break and recovery) was measured followed by positive fusional vergence (PFV) ranges (blur, break and recovery). Results: Pearson correlations were calculated and showed no correlations between vergence facility and any of fusional vergence measurements (p>0.05). Also, there were no significant differences of vergence facility measurements on the compensating vergence that passed or failed Sheard and Morgan's criterion for comfortable vision (p>0.05). Conclusions: There was no correlation between vergence facility and fusional vergence among symptomatic subjects. Hence, both vergence facility and fusional vergence should be assessed for those with binocular dysfunction in order to make an accurate diagnosis and management plan.

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Case of Anterior Segment Ischemia after Two Vertical Rectus Muscles Surgery (두 개의 수직근수술 후 발생한 전안부허혈 1예)

  • Ahn, Jayoung;Kim, Gyu Nam;Kim, Seong Jae;Chung, In Young;Seo, Seong Wook;Yoo, Ji Myong
    • Journal of The Korean Ophthalmological Society
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    • v.59 no.12
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    • pp.1195-1200
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    • 2018
  • Purpose: To report a case of anterior segment ischemia after superior and inferior rectus muscle surgery in a patient with superior rectus muscle enlargement. This is the first report in the Republic of Korea of anterior segment ischemia after two rectus muscles surgery. Case summary: An 80-year-old male was referred to our clinic with a 3 years history of diplopia. The patient had 30 prism diopters right eye hypertropia in the primary position and downgaze limitation. The orbital computed tomography scan revealed enlargement of the right superior rectus muscle. He underwent right superior rectus recession and inferior rectus resection. On postoperative day 2, slit lamp examination revealed diffuse corneal edema, Descemet's membrane folding, an aqueous flare and a dilated pupil. Treatment with 45 mg oral steroid was initiated, and 1% prednisolone acetate and 0.5% Levofloxacin eye drops were administered. At postoperative 1 month, the patient was orthophoric in the primary position, and there was no corneal edema, pupil abnormality or aqueous flare. Conclusions: Base on the present case, the possibility of anterior segment ischemia should be considered after even two muscles surgery, and older patients with vertical muscle surgery should be considered more carefully.