• Food Science of Animal Resources
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• v.37 no.6
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• pp.917-925
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• 2017

Whey protein, a by-product of milk curdling, exhibits diverse biological activities and is used as a dietary supplement. However, its effects on stress-induced vascular aging have not yet been elucidated. In this study, we found that whey protein significantly inhibited the Ang II-primed premature senescence of vascular smooth muscle cells (VSMCs). In addition, we observed a marked dose- and time-dependent increase in SIRT1 promoter activity and mRNA in VSMCs exposed to whey protein, accompanied by elevated SIRT1 protein expression. Ang II-mediated repression of SIRT1 level was dose-dependently reversed in VSMCs treated with whey protein, suggesting that SIRT1 is involved in preventing senescence in response to this treatment. Furthermore, resveratrol, a well-defined activator of SIRT1, potentiated the effects of whey protein on Ang II-primed premature senescence, whereas sirtinol, an inhibitor of SIRT1, exerted the opposite. Taken together, these results indicated that whey protein-mediated upregulation of SIRT1 exerts an anti-senescence effect, and can thus ameliorate Ang II-induced vascular aging as a dietary supplement.

• Korean Journal of Ecology and Environment
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• v.33 no.3 s.91
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• pp.206-212
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• 2000

Since some cyanobacterial isolates under selective culturing conditions are lacking of characteristic specialized cells or showing altered morphology, the morpho-taxonomic criteria are not accurate enough to discriminate between species. Instead of morphological parameters, a method based on the single or the combination of repetitive oligonucleotides in a single PCR, repetitive oligonucleotides-primed PCR (ROP-PCR), was applied to generate DNA profiles for members of the cyanobacterial genera Anabaena and Oscillatoria, both of which are responsible for causing poisonous blooms in various freshwater systems. ROP-PCR performed on 10 isolates of the cyanobacteria with ERIC and REP sequences from gram-negative bacteria, STRR1A and LTRR sequences derived from cyanobacterial genome, and eukaryotic repetitive sequences, led to the identification of distinct genotypes, and provided specific and repeatable DNA fingerprints for cyanobacterial isolates. Grouping analysis of cyanobacterial isolates showed a signifiant difference depending on the primer used in PCR.

• Journal of Embryo Transfer
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• v.4 no.1
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• pp.46-55
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• 1989

The effects of an antiprogesterone (RU 486) and an antiestrogen (tamoxifen) on ovulatory response and oocyte morphology were examined in pregnant mare serum gonadotropin (PMSG)-primed immatare female rats (28 days of age): a comparison has been made on two different regirnens primed with a "control" dose (4 IU) and a "superovulatory" dose (40 IU) of PMSG. Females for control control regimen received three consecutive injections of lmg RU486, lmg tamoxifen, or vehicle at 24, 36 and 48hr, and were killed at 72l'r after PMSG. Animals for superovalatory regimen received lmg RU486, 2.5mg tamoxifen, or vehicle fouowlag the injection schedule comparable to control regimen, and were killed at 60 and 72hr after PMSG. Compared to vehicle group, there was a significant reduction in ovulatory response as judged by the proportion of rats ovulating andi or by the mean number of oocytes per rat for each treatment of RU486 and tamoxifen in both regimens. The activity of tamoxifen in inhibiting the ovulatory response was greater in control, but less in superovulatory regimen than that of RU486 based on the dose employed for each antisteroid. In both regimens, RU 486 did not have any effect 6n the changes in the proportion of degenerate oocytes as well as ovarian weight, well tamoxifen treatment resulted in a marked promotion of oocyte degeneration as well as a great reduction in ovarian weight, compared to each parameter of vehicle group. RU486 treatment in each regimen did not alter the serum levels of any steroid hormones observed. Howerver, tamoxifen treatment was associated with significant increases in serum 17$\beta$-estradiol and decreases in progesterone in both regimens; also significant increases in androgens in superovulatory regimen. The results illustrate the relative inhibitory activity of RU486 and tamoxifen indicating major steroid hormone involved in PMSG-induced ovulation: 17$\beta$-estradiol for control and progesterone for superovulatory regimen. It also appears that tamoxifen-associated elevation of circulating 17$\beta$-estradiol andi or androgens could be in part, a contributing factor to the promotion of oocyte degeneration presumably by producing a hostile oviductal environment after ovulation.ent after ovulation.

• Journal of Korean Society of Forest Science
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• v.109 no.1
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• pp.41-49
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• 2020

In this study, the effect of seed pre-treatments and pellet coating materials to enhance the efficiency of large-scale propagation of Aster koraiensis seeds were investigated. Seeds were immersed in water for one day, and only those that sank were used for pre-treatment to use filled seeds. Pre-treatments were divided into hormone treatments, with gibberellic acid (GA₃; 200 and 500 ppm) and 24-epibrassinolide (10^-6, 10^-7, and 10^-8M), and priming with potassium nitrate (100 mM of KNO₃). To produce pellet-coated seeds, pellet materials (DTCS or DTK) were applied to control (unprimed) and primed seeds with binders (PVA or CMC). The maximum germination percent (GP) of seeds before pellet coating was 65% (with the priming treatment), and there was no difference in the GP of seeds among hormone treatments. For seeds sown in a growth chamber on filter paper, GP was 41% for control (unprimed/uncoated) seeds, 65% for uncoated primed seeds, 71% for DTCS/PVA-pellet-coated seeds, and 42% for DTK/CMC-pellet-coated seeds. Seeds that were primed first and then pellet-coated showed greatly improved GP, mean germination time (MGT), and germination rate than seeds that were only pellet-coated. For seeds sown in commercial soil in a greenhouse, control seeds had a GP of 27%, whereas primed seeds had the highest GP (58%), and their MGT and GT were 9.4 days and 7.0%·day, respectively. In addition, DTK/PVA-pellet-coated seeds (40%) also had a GP higher than the control (27%), and their MGT was 15-27 days. For seeds sown in sandy-loam soil in a greenhouse, unprimed-pellet-coated seeds and primed-pellet-coated seeds both had GPs ranged of 39%, which were lower than that of control seeds. In general, the seeds that were pellet-coated with DTK had a higher GP than those pellet-coated with DTCS. Furthermore, the MGT of unprimed-pellet-coated seeds was 15.0-19.8 days, which was longer than the MGT of primed-pellet-coated seeds. These results suggest that priming enhances seedling emergence of Aster koraiensis seeds. Moreover, when priming is combined with pellet coating, DTK is a more suitable pellet material than DTCS, and PVA and CMC are equally suitable adhesives.

• Journal of Life Science
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• v.13 no.4
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• pp.433-440
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• 2003

Osmotic and solid matrix priming treatments enhanced germination performance. We compared osmotic with solid matrix priming to determine the more effective treatment for improving seed germination in pepper and tomato. Seed hydration was immediately observed after osmotic priming and solid matrix priming treatment. The moisture content of solid matrix primed seeds was lower than that of osmotic primed seeds in the two vegetable crops. Osmotic priming and solid matrix priming did not increased percent germination, but showed shorter number of days to 50% of the final germination percentage ($T_{50}$) compared with untreated seeds, regardless of germination temperature. $T_{50}$ value was reduced in osmotic or solid matrix primed pepper seeds about 6.0, 5.0, 4.6 and 4.0 days compared with untreated seeds at 15, 20, 25, and $30^{\circ}C$, respectively. While, that in tomato seeds was reduced about 3.3, 5.0, 4.6 and 4.0 days compared with untreated seeds at 15, 20, 25, and $30^{\circ}C$, respectively. The effectiveness of osmotic priming or solid matrix priming in reducing the $T_{50}$ was greater when the seeds were germinated at $15^{\circ}C$ than at temperature of higher than $20^{\circ}C$. Solid matrix primed seeds germinated faster than osmotic primed seeds at all temperature in pepper. However, there was no significant difference on the percentage germination between solid matrix and osmotic primed seeds in tomato. After priming, dried-bark seeds showed faster germination than surface-dried seeds in pepper. On the other hand, there was no significant difference in tomato. Emergence of pepper and tomato seeds was markedly enhanced by osmotic priming or SMP treatment although the final emergence percentage was not significantly influenced. On the other hand, early growth was not significantly influenced by osmotic priming or SMP treatment of pepper and tomato seeds.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.4
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• pp.277-284
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• 2006

Seed priming is a useful technique for early establishment of seedling. In this experiment, the conditions for priming of pasture seeds (tall fescue, orchardgrass, alfalfa and white clover) have been optimized to ensure an early germination and more uniform growth of seedlings. The experiment was conducted in a split plot design with three replications. The main plots consisted of five different materials such as $KNO_3,\;KH_2PO_4,\;K_3PO_4,\;Ca(NO_3){_2}$ and PEG. The subplots were consisted of three chemical concentration groups such as 500mM (or 10%), 100mM (or 20%) and 200mM (or 30%). Effect of priming materials and its concentrations were different in all four pasture seeds examined. Chemical concentration did not show any significant effects on the germination of tall fescue, however, germination of tall fescue primed with $Ca(NO_3){_2}\;and\;K_3PO_4$ materials were higher than the others. The concentration of prime materials did not affect on the germination of orchardgrass, but germination of orchardgrass primed with $KH_2PO_4$ was showed better result than the other materials examined. The seeds of alfalfa and white clover primed with PEG showed better germination capability than the other priming materials. In addition, chemical concentration of primed seeds also showed significant difference on the germination of alfalfa and white clover. These results suggest that seed priming induced earlier and higher germination for all four pastures tested. However, the, degree of priming effectiveness on pasture species depending on the priming materials and its concentrations.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.214-214
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• 2017

Seeds of Tomato (Lycopersicon esculentum Mill.) have demanded high quality because of their high cost of seed. The optimization of the seed priming techniques that have positive effect on fast and uniform germination becomes important at the commercial level. Several factors such as solution composition, osmotic potential, and treatment duration affect seed priming response. In this study, osmotic potentials of priming solution and germination characteristics of primed seed were investigated to clarify the effects different inorganic salt types and the duration. Tomato seeds were primed in osmotic solutions that were osmotic potential ranged -1.54 to -0.45 MPa in an aerated solution of PEG 8000 (17%, 22%, 27%), and inorganic salt solution of $KNO_3$, $Na_2SO_4$, and $K_2SO_4$ (100, 200, 300mM). The seeds were treated at $20^{\circ}C$ for 2, 4, and 6 days. After each treatment, the seeds were dried to moisture content ranged 5-8% at $25^{\circ}C$. Four replications of 25 seeds per each treatments were placed in 10-cm petri dishes containing two filter papers and 3 ml of $dH_2O$ and incubated at $20^{\circ}C/30^{\circ}C$ and $15^{\circ}C$ and seedlings evaluated for abnormality after 14 days of incubation. Seed water potential (${\psi}$) was correlated with water potential of priming solution ($r^2=0.86$). Seeds primed in 100mM $KNO_3$ resulted the highest germination rate (GR, $63.9 %{\cdot}day^{-1}$) and lowest mean germination time (MGT, 2.0 days) comparing to untreated control ($23.9%{\cdot}day^{-1}$ of germination rate and 4.1 days of MGT) at $20/30^{\circ}C$, even though 96% of germination percentage were not different. Seeds primed in 100mM $KNO_3$ (${\psi}=-0.45MPa$) for 4 days showed ${\psi}=-0.38MPa$. Priming in $Na_2SO_4$, $K_2SO_4$, and PEG solution for 6 days improved MGT and GR, but not significantly than 4 days of treatment. Additionally, stepwise osmotic solution treatment with 100mM and 300mM concentration for 6 day did not showed differences with single treatment. In relation to osmotic potentials, identical osmotic potential in different inorganic salt solution showed different effect on germination characteristics.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.216-216
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• 2017

Primed seeds germinate faster and uniformly, since these have already started imbibition and metabolism requiring to complete germination. Several factors such as solution composition, osmotic potential, and treatment duration affect seed priming response. Water potentials of priming solution and germination response of primed seed were investigated to clarify the effects different inorganic salt types and the duration. Pepper seeds were primed in osmotic solutions that were osmotic potential ranged -1.54 to -0.45 MPa in an aerated solution of polyethylene glycol 8000 (PEG; 17%, 22%, 27%), and inorganic salt solution of $KNO_3$, $K_2SO_4$, and $Na_2SO_4$ (100, 200, 300mM) in aerated condition. The seeds were treated at $20^{\circ}C$ for 4, 6 day (D) and stepwise duration combined concentration of 100mM (2 or 4 days) and 300mM(2 or 4days). After soaking treatment, seeds were washed with distilled water and then were dried to approximately 6% moisture content in dry chamber at $25^{\circ}C$. The germination of seed was characterized by ISAT rules at $20^{\circ}C/30^{\circ}C$ and $15^{\circ}C$. Total germination percentage (GP), mean germination time (MGT), germination uniformity (GU), germination rate (GR), and health seedling percent (HS) were calculated on the germinated seed in a 14-day period. Seed water potential (${\psi}$) was correlated with water potential of priming solution ($r^2=0.84$). The effect of seed priming on germination varies with inorganic salt and organic agents (PEG). Germination percentage (GP, 77 and 73%), GR (21.3 and $19.4 %{\cdot}day^{-1}$), and HS (58 and 52 %) was greatest and lowest MGT (4.2 and 4.8 day) when they were primed in 100mM $K_2SO_4$ (${\psi}=-0.45MPa$), and 100mM $KNO_3$ (${\psi}=-0.52MPa$) for 6 days compared to untreated control (67% GP, $19.7%{\cdot}day^{-1}\;GR$, 18% HS, and 10.8 day MGT) in $15^{\circ}C$ chamber. Water potential less then -1.2MPa of osmotic solution resulted lower GP, GR and HS and in $K_2SO_4$ and $KNO_3$. However, stepwise treatment of water potential that changed solution as 100mM for 4 D + 300mM for 2 D or 300mM for 4 D + 100mM for 2 D) were not better than single concentration alone in germination characteristics.

• IMMUNE NETWORK
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• v.6 no.2
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• pp.93-101
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• 2006

Background: Memory T lymphocytes of the immune system provide long-term protection in response to bacterial or viral infections/immunization. Ag concentration has also been postulated to be important in determining whether T cell differentiation favors effector versus memory cell development. In the present study we hypothesized that naive Ag-specific $CD4^+$ T cells briefly stimulated with different Ag doses at the primary exposure could affect establishment of memory cell pool after secondary immunization. Methods: To assess this hypothesis, the response kinetics of DO11.10 TCR $CD4^+$ T cells primed with different Ag doses in vitro was measured after adoptive transfer to naive BALB/c mice. Results: Maximum expansion was shown in cells primarily stimulated with high doses of ovalbumin peptide $(OVA_{323-339})$, whereas cells in vitro stimulated with low dose were expanded slightly after in vivo secondary exposure. However, the cells primed with low $OVA_{323-339}$ peptide dose showed least contraction and established higher number of memory cells than other treated groups. When the cell division was analyzed after adoptive transfer, the high dose Ag-stimulated donor cells have undergone seven rounds of cell division at 3 days post-adoptive transfer. However, there was very few division in naive and low dose of peptide-treated group. Conclusion: These results suggest that primary stimulation with a low dose of Ag leads to better memory $CD4^+$ T cell generation after secondary immunization. Therefore, these facts imply that optimally primed $CD4^+$ T cells is necessary to support effective memory pool following administration of booster dose in prime-boost vaccination.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.4
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• pp.373-381
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• 2017

The main objective of this study was to increase the germination percentage of kenaf seeds with less number of times under non-saline and saline conditions. Therefore, the first goal was to assess the response of kenaf seeds to NaCl. The second goal was to evaluate the effects of $KNO_3$ on kenaf seed germination. The germination percentage exhibited a decreasing tendency in germination rate. Plant dry weight was approximately 0.2 g in all treatments at 5 days after germination. As time passed, the electrical conductivity (EC) value of hydro-priming (HP) consistently increased by 8.7 mS/cm at 24 hours of immersion. However, seeds primed with $KNO_3$ showed no difference in EC values even as times passed. Regarding the priming effect, priming in 100 mM $KNO_3$ concentration for 12 hours increased germination up to 85% in $H_20$ solution and in 0 mM $KNO_3$ concentration upto 73.8% under 0.3% NaCl solution, compared to that of Control. Germination synchronization, shoot length, and leaf unfolding of primed seeds were greater than those of the Control. In addition, main root and hair roots appeared more rapidly in the treated seeds and were more abundant compared to that of the Control. The T50 (times to reach 50% of the final germination percentage) of the Control in both $H_20$ and 0.3% NaCl solutions was 18 and 22 hours, respectively. However, when treated $KNO_3$ priming (0 to 100 mM) in $H_20$ and 0.3% NaCl solution, 9 hours was sufficient to reach T50. Primed (hydro-priming and $KNO_3$) seeds had a lower MDG (mean days untill germination; 0.6-0.62) compared to that of the Control (1.13-1.31) in $H_20$ and 0.3% NaCl solutions. Regarding dry weight of plants after priming, an increasing tendency after the priming treatment in the H20 solution was observed. Furthermore, no significant difference in plant dry weight under 0.3% NaCl stress was observed between the Control and primed seeds. Taken together, the results suggest that 50-100 mM $KNO_3$ priming for 24 hours optimize seed germination rate in less number of times of exposure with great vigor. Therefore, it is recommended for kenaf seed invigoration before planting.