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http://dx.doi.org/10.5851/kosfa.2017.37.6.917

Whey Protein Attenuates Angiotensin II-Primed Premature Senescence of Vascular Smooth Muscle Cells through Upregulation of SIRT1  

Hwang, Jung Seok (Department of Food Science and Biotechnology of Animal Products, Sanghuh College of Life Sciences, Konkuk University)
Han, Sung Gu (Department of Food Science and Biotechnology of Animal Products, Sanghuh College of Life Sciences, Konkuk University)
Lee, Chi-Ho (Department of Food Science and Biotechnology of Animal Products, Sanghuh College of Life Sciences, Konkuk University)
Seo, Han Geuk (Department of Food Science and Biotechnology of Animal Products, Sanghuh College of Life Sciences, Konkuk University)
Publication Information
Food Science of Animal Resources / v.37, no.6, 2017 , pp. 917-925 More about this Journal
Abstract
Whey protein, a by-product of milk curdling, exhibits diverse biological activities and is used as a dietary supplement. However, its effects on stress-induced vascular aging have not yet been elucidated. In this study, we found that whey protein significantly inhibited the Ang II-primed premature senescence of vascular smooth muscle cells (VSMCs). In addition, we observed a marked dose- and time-dependent increase in SIRT1 promoter activity and mRNA in VSMCs exposed to whey protein, accompanied by elevated SIRT1 protein expression. Ang II-mediated repression of SIRT1 level was dose-dependently reversed in VSMCs treated with whey protein, suggesting that SIRT1 is involved in preventing senescence in response to this treatment. Furthermore, resveratrol, a well-defined activator of SIRT1, potentiated the effects of whey protein on Ang II-primed premature senescence, whereas sirtinol, an inhibitor of SIRT1, exerted the opposite. Taken together, these results indicated that whey protein-mediated upregulation of SIRT1 exerts an anti-senescence effect, and can thus ameliorate Ang II-induced vascular aging as a dietary supplement.
Keywords
angiotensin II; senescence; SIRT1; vascular smooth muscle cell; whey protein;
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