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Effect of Priming Materials and its Concentrations on the Germination of Pasture Seed

Priming 약제의 종류와 농도가 목초종자의 발아에 미치는 영향

  • Published : 2006.12.30

Abstract

Seed priming is a useful technique for early establishment of seedling. In this experiment, the conditions for priming of pasture seeds (tall fescue, orchardgrass, alfalfa and white clover) have been optimized to ensure an early germination and more uniform growth of seedlings. The experiment was conducted in a split plot design with three replications. The main plots consisted of five different materials such as $KNO_3,\;KH_2PO_4,\;K_3PO_4,\;Ca(NO_3){_2}$ and PEG. The subplots were consisted of three chemical concentration groups such as 500mM (or 10%), 100mM (or 20%) and 200mM (or 30%). Effect of priming materials and its concentrations were different in all four pasture seeds examined. Chemical concentration did not show any significant effects on the germination of tall fescue, however, germination of tall fescue primed with $Ca(NO_3){_2}\;and\;K_3PO_4$ materials were higher than the others. The concentration of prime materials did not affect on the germination of orchardgrass, but germination of orchardgrass primed with $KH_2PO_4$ was showed better result than the other materials examined. The seeds of alfalfa and white clover primed with PEG showed better germination capability than the other priming materials. In addition, chemical concentration of primed seeds also showed significant difference on the germination of alfalfa and white clover. These results suggest that seed priming induced earlier and higher germination for all four pastures tested. However, the, degree of priming effectiveness on pasture species depending on the priming materials and its concentrations.

종자의 priming은 유식물의 초기 정착에 중요한 기술 중의 하나이다. 따라서 본 시험에서는 목초의 주요 초종인 톨 페스큐, 오처드그라스, 알팔파 및 화이트 클로버의 발아율 향상과 균일도 증가를 위하여 priming 약제의 종류와 농도를 달리하여 발아율을 조사하였다. 본 시험은 분할구 배치법으로 주구는 $KNO_3,\;KH_2PO_4,\;K_3PO_4,\;Ca(NO_3){_2}$ 및 PEG 약제를, 세구는 50mM(또는 10%), 100mM(또는 20%) 및 200mM(또는 30%)의 약제농도를 두었다. Priming 목초의 발아율은 약제의 종류 및 농도에 따라 다르게 나타났다. 화본과 목초인 톨 페스큐는 $Ca(NO_3){_2}$$K_3PO_4$가 다른 약제보다 발아율이 높았으며, 오처드그라스는 약제간에 차이가 없었으나 $KH_2PO_4$가 다른 약제보다 발아율이 높았다. 한편 두과목초인 알팔파와 화이트 클로버는 모두 PEG에서 높은 발아율을 나타내었다. 한편 약제의 농도는 약제의 종류에 따라 달랐다. 이상의 결과를 종합해 보면 목초의 priming은 초종에 따라 적정 약제종류와 농도가 달랐다.

Keywords

References

  1. Association of Official Seed Analysis. 1983. Rules for testing seeds. Proc. Assoc. Seed Anal. 54:1-112
  2. Bodsworth, S. and J.D. Bewley. 1981. Osmotic priming of crop species with polyethylene glycol as a means of enhancing early and synchronous germination at cool temperature. Can. J. Bot. 59:672-676 https://doi.org/10.1139/b81-094
  3. Bradford, K.J. 1986. Manipulation of seed water relations via osmotic priming to improve germination under stress condition. Hort Science 26: 1105-1112
  4. Brocklehurst, P.A. and J. Dearman. 1983. Interaction between seed priming treatments and nine seed lots of carrot, celery and onion. I. Laboratory germination. Ann. Appl. Biol. 102:577-584 https://doi.org/10.1111/j.1744-7348.1983.tb02729.x
  5. Come, D. and T. Tissaoui. 1973. Interrelated effects of imbibition, temperature and oygen on seed germination. p. 157-168. In: W. Heydecker (ed.). Seed ecology. Butterworths, London
  6. Coolber, P., A. Francis and D. Grierson. 1984. The effect of low temperature pre-sowing treatment on the germination performance and membrane integrity of artificially aged tomato seeds. J. Exp. Bot. 35:1609-1617 https://doi.org/10.1093/jxb/35.11.1609
  7. Dahal, P., K.J. Bradford and R.A. Jones. 1990. Effects of priming and endosperm Integrity on seed germination rates of tomato genotype. II. Germination at reduced water potential. J. Exp. Bot. 41: 1441-1453 https://doi.org/10.1093/jxb/41.11.1441
  8. Fu, J.R., X.H. Lu, R.Z. Chen, B.Z. Zhang, Z, S. Liu, Z.S. Li and D.Y. Cay. 1988. Osmoconditioning of peanut (Arachis lypogea L.) seeds with PEG to improve vigor and some biochemical activities. Seed Sci. Technol. 16:197-212
  9. Fleming, R.L. and S.A. Lister. 1984. Stimulation of black spruce germination by osmotic priming: Laboratory studies. Information Report O-X-362. Canadian Forestry Service
  10. Greenway, H. and R. Munns. 1980. Mechanism of salt tolerance in nonhalophytes. Ann. Rev. Plant Physiol. 31:149-190 https://doi.org/10.1146/annurev.pp.31.060180.001053
  11. Haigh, A.M. and E.W.R. Barlow. 1987. Water relations of tomato seed germination. Aust. J. Plant Physiol. 114:485-492
  12. Hegarty, T.W. and H.S. Ross. 1980/1981. Investigations of control mechanisms of germination under water stress. Israel J. Bot. 29:83-92
  13. Heydecker, W. 1974. Germination of an idea: the priming of seeds. University of Nottingham School of Agriculture Report, 197/1974:50-57
  14. Heydecker, W. 1978. Stress and seed germination : An agronomic view. p. 237-282. In A.A. Khan (ed.). The physiology and biochemistry of seed dormancy and germination. Elsevier/North-Holland, Amsterdam
  15. Heydecker, W. and P. Coolbear. 1977. Seed treatments for improved performance survey and attempted prognosis. Seed Sci. & Technol. 5:353-425
  16. Khan, A.A., K.L. Tao, J.S. Knypl, B. Borkowska, and L. E. Powell. 1978. Osmotic conditioning of seeds; physiological and biochemical changes. Acta Hort. 83:267-278
  17. Khan, A.A. 1992. Preplant physiological seed conditioning. Hort. Rev. 13:131-181
  18. Liptay, A. and C.S. Tan. 1985. Effect of various levels of available water on germination of polyethylene glycol (PEG) pretreated of untreated tomato seeds. J. Amer. Soc. Hort. Sci. 110: 748-751
  19. Malnassy, P.G. 1971. Physiological and biochemical studies on a treatment hastening the germination of seeds at low temperatures. Ph.D. Diss. Rutgers Univ., New Brunswick, N.J
  20. Mexal, J., J.T. Fisher, J. Osteryoung, and C.P.P. Reid. 1975. Oxygen availability in polyethylene glycol solutions and its implications in plant-water relations. Plant Physiol. 55:20-24 https://doi.org/10.1104/pp.55.1.20
  21. Rabin, J., G.A. Berkowitz, and S.W. Akers, 1988. Field performance of osmotically primed parsley seed. Hort. Science 23:554-555
  22. Stoffela, P.J., M. Lipucci, Di Paola, A. Pardossi, and F. Tognomi. 1992. Seedling root morphology and shoot growth after seed priming or pregermination of bell pepper. Hort. Science 27: 214-215
  23. Suzuki, H., S. Obayash, J. Yamagish, and S. Inanaga. 1990. Effect of pH of tertiary phosphates solutions on radicals protrusion during priming of carrot seeds. J. Japan Soc. Hort. Sci. 62:143-148
  24. Taylor, A.G. and G.E. Harman. 1990. Concepts and technologies of selected seed treatment. Ann. Rev. Phytopathol. 28:321-339 https://doi.org/10.1146/annurev.py.28.090190.001541