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The Korean Association of Immunobiologists (대한면역학회)
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The Kinetics of Secondary Response of Antigen-Specific $CD4^+$ T Cells Primed in vitro with Antigen

실험적으로 항원에 의하여 일차 자극된 $CD4^+$ T 세포의 이차 면역 반응의 분석

  • Park, Seong-Ok (Laboratory of Microbiology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Han, Young-Woo (Laboratory of Microbiology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Aleyas, Abi George (Laboratory of Microbiology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • George, June Abi (Laboratory of Microbiology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Yoon, Hyun-A (Laboratory of Microbiology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Eo, Seong-Kug (Laboratory of Microbiology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
  • 박성옥 (전북대학교 수의과대학 미생물학교실 및 생체안전성연구소) ;
  • 한영우 (전북대학교 수의과대학 미생물학교실 및 생체안전성연구소) ;
  • ;
  • ;
  • 윤현아 (전북대학교 수의과대학 미생물학교실 및 생체안전성연구소) ;
  • 어성국 (전북대학교 수의과대학 미생물학교실 및 생체안전성연구소)
  • Published : 2006.06.30
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Abstract

Background: Memory T lymphocytes of the immune system provide long-term protection in response to bacterial or viral infections/immunization. Ag concentration has also been postulated to be important in determining whether T cell differentiation favors effector versus memory cell development. In the present study we hypothesized that naive Ag-specific $CD4^+$ T cells briefly stimulated with different Ag doses at the primary exposure could affect establishment of memory cell pool after secondary immunization. Methods: To assess this hypothesis, the response kinetics of DO11.10 TCR $CD4^+$ T cells primed with different Ag doses in vitro was measured after adoptive transfer to naive BALB/c mice. Results: Maximum expansion was shown in cells primarily stimulated with high doses of ovalbumin peptide $(OVA_{323-339})$, whereas cells in vitro stimulated with low dose were expanded slightly after in vivo secondary exposure. However, the cells primed with low $OVA_{323-339}$ peptide dose showed least contraction and established higher number of memory cells than other treated groups. When the cell division was analyzed after adoptive transfer, the high dose Ag-stimulated donor cells have undergone seven rounds of cell division at 3 days post-adoptive transfer. However, there was very few division in naive and low dose of peptide-treated group. Conclusion: These results suggest that primary stimulation with a low dose of Ag leads to better memory $CD4^+$ T cell generation after secondary immunization. Therefore, these facts imply that optimally primed $CD4^+$ T cells is necessary to support effective memory pool following administration of booster dose in prime-boost vaccination.

Keywords

References

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