• Journal of Physiology & Pathology in Korean Medicine
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• v.33 no.3
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• pp.175-180
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• 2019

Torilis Japonica (TJ) has been used as an anti-allergy, antifungal, and antibacterial agent. Recent studies have reported that it also shows anti-cancer effects. It is report that TJ inhibits melanin synthesis in melanocyte in the skin. However, the effect and mechanism of TJ extract (TJE) on Ultraviolet (UV)B-induced photoaging are unknown. In this study, we investigated the preventive effects of TJE on matrix metalloproteinase (MMP)-1 and MMP-3 expressions and the underlying molecular mechanism in UVB-irradiated primary human dermal fibroblasts (HDFs). The effect of TJE on HDF cell viability was determined using the XTT assay and cell counting. MMP-1 and MMP-3 expressions levels were measured by western blotting and real-time PCR analysis. Activations of mitogen-activated protein kinase (MAPKinase), nuclear $factor-{\kappa}B$ ($NF-{\kappa}B$), and activator protein-1(AP-1) were measured by western blotting. Our results showed that TJE effectively reduced UVB-induced MMP-1 and MMP-3 protein and mRNA levels. Moreover, TJE significantly blocked the UVB-induced activation of MAPK (p38 and JNK) and transcription factors ($NF-{\kappa}B$ and AP-1), but not ERK. Taken together, our results suggest that the TJE inhibits UVB-induced MMP expressions in HDFs and its may be a potential agent for the prevention and treatment of skin photoaging.

• The Journal of the Convergence on Culture Technology
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• v.5 no.2
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• pp.123-138
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• 2019

The clinical institution of some dental hygiene students and the degree of practice and performance according to the practice contents are identified, so as to develop clinical practice guidelines and to improve the education of universities and practical institutions. From November 2016 to March 2017, the questionnaire was prepared by self-inclusion after explaining the purpose of research and how to prepare questionnaire to some dental hygiene students. This material has been analyzed using PASW Statistics 18.0. As for the clinical practice content items of basic care and infection control, the frequency of "execution" among practice methods was high, and the distribution rate was high in oral evil face radiation, preventive dentistry, prosthesis, periodontology, orthodontics, and oral internal medicine. The conservation department has conducted various practical methods such as "executing," "observing" and "preparing." Oral surgery showed similar distribution rates, such as "observation," "preparation," and "execution" in the "preparation and cooperation of the feet." In pediatric dentistry, "observation" was the most common practice, and among them, "observation," "preparation," and "execution" were performed in "preparation and cooperation for infantile development." In addition, students with more experience in clinical practice showed statistically significant differences in basic care, oral hygiene, preventative dentistry, conservation, dental dentistry, oral surgery, orthodontics, and oral internal medicine(p<0.05). Most clinical practice shows that it is more observable than performed.It is expected that the goal of clear clinical practice content should be set by grade or number of clinical practice experiences, as well as the practice method that can be performed by the trainees.

• Journal of Society of Preventive Korean Medicine
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• v.5 no.2
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• pp.106-113
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• 2001

The Cytotoxic activity of the ethyl acetate soluble fraction of Perilla frutescens on human oral epithelioid carcinoma cell lines was evaluated by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl-2H-tetrazalium bromide (MTT) colorimetric method. The light microscopic study was carried out to observe the morphological changes of cultured human oral epitheloid carcinoma cell lines (KB) These results were obtained as follows; 1. subfraction 1 of the ethyl acetate soluble fraction of perilla frutescens was shown significant cytotoxic activity (p<0.001) at 10-2 mg/ml concentration, this concentration was decreased the cytotoxic activity of 70.3% . 2. The comparison of IC50 values of various subfractions in human oral epithelioid carcinoma cell lines was exhibited that their susceptibility to these subfractions decreased in the following order ; subfraction 5 > subfraction 4 > subfraction 3 > subfraction 2 > subfraction 1 by MTT assay. In light microscopy, the subfraction 1 of the ethyl acetate soluble fraction of Perilla frutescens showed the highest cytotoxic activity. These findings suggest that subfraction 1 possessed the most cytotoxic constituents.

• Journal of Society of Preventive Korean Medicine
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• v.5 no.2
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• pp.122-128
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• 2001

This study was carried out to evaluate antitoxic effects of Houttuynia cordata extract on cadmium by colorimetric methods. The antitoxic activity ofc in NIH 3T3 fibroblasts was evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) and SRB (sulforhodamine B protein) assays. The light microscopic study was carried out to observe morphological changes of the treated cells. The concentration of 10-2 mg/ml of Houttuynia cordata extract was shown significant antitoxic activity. The number of NIH 3T3 fibroblasts were antitoxic and tend to regenerate. These results suggest that the ethyl acetate extract of Houttuynia cordata retains a potential antitoxic activity.

• Journal of Korean society of Dental Hygiene
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• v.16 no.2
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• pp.225-231
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• 2016

Objectives: The purpose of this study was to investigate the influential factors of stress and fatigue on peridontal indicators in patients with periodontal disease. Methods: A self-administrated questionnaire study was conducted during November 2012 to January 2013 with 140 periodontal patients. The Fatigue and Stress were evaluated with Multidimensional Fatigue Scale(MFS) and Psychosocial Well-bing Index(PWI). Periodontal health indicators were consisted with gingival index and pocket depth. Data were analyzed by descriptive analysis, t-test, ANOVA, and multiple regression analysis. Results: Overall stress and fatigue level of subjects were $22.61{\pm}5.62$ and $64.67{\pm}9.45$. Gingival index and periodontal pocket depth as periodontal health indicators were $1.26{\pm}0.3$ and $4.29{\pm}0.52$. The influential factors of gingival index and periodontal pocket depth were social function(subcategory of pwi) and depression(subcategory of pwi), respectively. Conclusions: Some subcategories of PWI were related with periodontal health indicators. So, consideration of stress related with depression and social function are needed to manage and prevent periodontal disease.

• Journal of Preventive Medicine and Public Health
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• v.50 no.3
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• pp.165-176
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• 2017

Objectives: This study aimed to estimate the risk of bleeding following minor oral surgical procedures and uninterrupted aspirin therapy in high-risk patients or patients with existing chronic diseases compared to patients who did not use aspirin during minor oral surgery at a public hospital. Methods: This retrospective cohort study analyzed the data of 2912 patients, aged 20 years or older, who underwent 5251 minor oral surgical procedures at a district hospital in Thailand. The aspirin group was comprised of patients continuing aspirin therapy during oral surgery. The non-aspirin group (reference) included all those who did not use aspirin during surgery. Immediate and late-onset bleeding was evaluated in each procedure. The risk ratio of bleeding was estimated using a multilevel Poisson regression. Results: The overall cumulative incidence of immediate bleeding was 1.3% of total procedures. No late-onset bleeding was found. A significantly greater incidence of bleeding was found in the aspirin group (5.8% of procedures, p<0.001). After adjusting for covariates, a multilevel Poisson regression model estimated that the bleeding risk in the aspirin group was 4.5 times higher than that of the non-aspirin group (95% confidence interval, 2.0 to 10.0; p<0.001). However, all bleeding events were controlled by simple hemostatic measures. Conclusions: High-risk patients or patients with existing chronic diseases who continued aspirin therapy following minor oral surgery were at a higher risk of hemorrhage than general patients who had not used aspirin. Nonetheless, bleeding complications were not life-threatening and could be promptly managed by simple hemostatic measures. The procedures could therefore be provided with an awareness of increased bleeding risk, prepared hemostatic measures, and postoperative monitoring, without the need for discontinuing aspirin, which could lead to more serious complications.

• Journal of Korean society of Dental Hygiene
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• v.12 no.1
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• pp.113-122
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• 2012

Objectives : This study was carried out to investigate the caries resistant effect of fluoride by dental polishing prior to fluoride application in vitro. Methods : Artificial caries lesion was made on the surface of specimen enamel taken from cow's permanent cuspid on the part of labial surface after resin embedding and polishing. Artificial dental plaque was formed on the 72 dental specimen 25~45 VHN(Vickers Hardness Number) which were divided into three groups(fluoride varnish, APF gel, control) with dental polishing and without polishing respectively. Fluoride varnish and APF gel group with 20 second polishing or without polishing were immersed in the artificial saliva respectively. Control group with or without polishing were immersed in the artificial saliva. Results : 1. Significant difference was not found by fluoride varnish between polishing group and non polishing group (p>0.01). 1) polishing group. The changes of Vickers Hardness Number(VHN) were $14.49{\pm}13.73$. 2) non-polishing group. The changes of VHN were $11.67{\pm}5.39$. 2. Significant difference was not found by APF gel between polishing group and non polishing group (p>0.01). 1) polishing group. The changes of VHN were $8.48{\pm}8.37$. 2) non-polishing group. The changes of VHN were $5.32{\pm}2.59$. Conclusions : Showed no significant difference between polishing group and non-polishing group regardless of fluoride materials (fluoride varnish, APF gel).

• Journal of Society of Preventive Korean Medicine
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• v.18 no.3
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• pp.23-39
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• 2014

Objective : This review aims at analyzing Complementary and Alternative Medicine (CAM) therapies, using for oral health to know current use of CAM in dental area by analyzing randomized controlled clinical trials (RCTs). Method : We searched the following 6 electronic databases until 1 July 2014. : Pubmed, Medline, Embase, Allied and Complementary Medicine Database(AMED), Cumulative Index to Nursing and Allied Health Literature(CINAHL), the Cochrane Library. We included parallel RCTs and cross-over RCTs that assessed the efficacy of CAM regardless of blinding and language. Results : A total of 154 RCT articles were included. There were 2-arm parallel study design(69.5%), 3-arm parallel study design(20.8%), 4-arm parallel study design(4.5%) and Cross-over design(5.2%). Complementary and alternative medicine RCTs in oral health tend to increase in the early-2000s and in the mid-2010s. In addition, 154 citations were classified according to diseases and interventions that categorized Natural Products studies(68.2%), Mind and Body Practices studies(31.8%). We classified in detail that vitamin and mineral therapies(29.9%), dietary supplements(24.7%), acupuncture(23.3%), Herbal medicine(13.6%), homeopathy (2.6%), energy therapies(2.6%), Massage(1.9%), biofeedback(0.6%), traditional medicine(0.6%). Conclusion : Increasing publications and diverse interventions regarding CAM for oral health is observed by analyzing RCTs from the literature review. Further studies are needed to be performed as systematic reviews to verify their effectiveness and the research to inquire into side effect.

• Korean Journal of Pharmacognosy
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• v.28 no.4
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• pp.264-270
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• 1997

This Study was carried out develop antitumor effect of the n-butanol soluble of fraction of Perilla frutescens on human skin melanoma cells. The antitumor activity of various fractions obtained form n-butanol soluble fraction of Perilla frutescens was evaluated in human skin melanoma cells. The antitumor activity of the n-butanol soluble fraction in human skin melanoma cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, neutral red (NR) assay and sulforhordamine B protein (SRB) assay of colorimetic assay methods. The light microscopic study was carried out to observe morphological changes of cultured human skin melanoma cells. These results were obtained follows; The fractions 5 and 6 of the n-butanol soluble fraction of P frutescens were shown significant antitumor activities. The number of human skin melanoma cells were decreased and tend to form cell cluster by treatment with actions 5 and 7 of the n-butanol soluble fraction of P. frutescens. The fraction 6 of the the n-butanol soluble fraction showed the highest antitumor activity on P. frutescens. These results suggest that the fraction 6 of the n-butanol soluble fraction of P. frutescens may be a valuable choice for the studies on the treatment of human skin tumors.

• Journal of Preventive Medicine and Public Health
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• v.56 no.4
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• pp.357-367
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• 2023

Objectives: The 2018 Basic Health Research (RISKESDAS), conducted by the Ministry of Health of the Republic of Indonesia showed a high prevalence of dental caries (88.8%) in Indonesia and suggested that smoking tobacco was associated with an increased risk of dental caries. This study analyzed the association between tobacco smoking and dental caries in the Indonesian population. Methods: This was a cross-sectional analysis of secondary data collected from RISKESDAS 2018. The study population included 35 391 Indonesians aged ≥10 years from all 34 provinces. The decayed, missing, and filled teeth (DMFT) index was used to measure dental caries. Smoking status was assessed qualitatively based on smoking activity, and the level of smoking exposure was assessed based on the Brinkman index. A multivariable logistic regression analysis was employed to examine the relationships of smoking status and smoking exposure levels with the DMFT index. Results: Of the population aged ≥10 years, 36% had a DMFT≥8 (females: 37.5%, males: 33.9%). Almost one-fourth (23.4%) were current smokers, and 4.1% were ex-smokers. Furthermore, 26.4% had a Brinkman index ≥400, indicating heavy smoking. According to the multivariate analysis, current smoking status was associated with the risk of DMFT≥8 in males (adjusted odds ratio [aOR], 1.40; 95% CI, 1.27 to 1.55; p<0.001) and overall (aOR, 1.07; 95% CI, 1.00 to 1.14; p=0.037). In females, ex-smoking was associated with a 41% higher risk of DMFT≥8 (aOR, 1.41; 95% CI, 1.07 to 1.84; p=0.014). Heavy smoking was associated with a higher risk of DMFT≥8 in males (aOR, 1.38; 95% CI, 1.25 to 1.52; p<0.001) and females (aOR, 1.24; 95% CI, 1.03 to 1.50; p=0.022). Conclusions: Tobacco smoking was associated with dental caries in the Indonesian population.