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http://dx.doi.org/10.15188/kjopp.2019.06.33.3.175

Extracts of Torilis Japonica Suppresses of Ultraviolet B-induced Matrix Metalloproteinase-1/-3 Expressions in Human Dermal Fibroblasts  

Noh, Eun Mi (Department of Oral Biochemistry, and Institute of Biomaterials, Implant, College of Dentistry, Wonkwang University)
Song, Hyun Kyung (Department of Oral Biochemistry, and Institute of Biomaterials, Implant, College of Dentistry, Wonkwang University)
Kim, Jeong Mi (Department of Oral Biochemistry, and Institute of Biomaterials, Implant, College of Dentistry, Wonkwang University)
Lee, Guem San (Department of Herbology, College of Korean Medicine, Wonkwang University)
Kwon, Kang Beom (Department of Korean Physiology, College of Korean Medicine, Wonkwang University)
Lee, Young Rae (Department of Oral Biochemistry, and Institute of Biomaterials, Implant, College of Dentistry, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.33, no.3, 2019 , pp. 175-180 More about this Journal
Abstract
Torilis Japonica (TJ) has been used as an anti-allergy, antifungal, and antibacterial agent. Recent studies have reported that it also shows anti-cancer effects. It is report that TJ inhibits melanin synthesis in melanocyte in the skin. However, the effect and mechanism of TJ extract (TJE) on Ultraviolet (UV)B-induced photoaging are unknown. In this study, we investigated the preventive effects of TJE on matrix metalloproteinase (MMP)-1 and MMP-3 expressions and the underlying molecular mechanism in UVB-irradiated primary human dermal fibroblasts (HDFs). The effect of TJE on HDF cell viability was determined using the XTT assay and cell counting. MMP-1 and MMP-3 expressions levels were measured by western blotting and real-time PCR analysis. Activations of mitogen-activated protein kinase (MAPKinase), nuclear $factor-{\kappa}B$ ($NF-{\kappa}B$), and activator protein-1(AP-1) were measured by western blotting. Our results showed that TJE effectively reduced UVB-induced MMP-1 and MMP-3 protein and mRNA levels. Moreover, TJE significantly blocked the UVB-induced activation of MAPK (p38 and JNK) and transcription factors ($NF-{\kappa}B$ and AP-1), but not ERK. Taken together, our results suggest that the TJE inhibits UVB-induced MMP expressions in HDFs and its may be a potential agent for the prevention and treatment of skin photoaging.
Keywords
Torilis Japonica(蛇 床 子 ); photoaging; MMPs; $NF-{\kappa}B$; AP-1;
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