Korean Journal of Pharmacognosy (생약학회지)
- Volume 28 Issue 4
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- Pages.264-270
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- 1997
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- 0253-3073(pISSN)
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- 2288-9299(eISSN)
Development of Anticancer Agents from Korean Medicinal Plants. Part 5 - Cytotoxic Activity of the Butanol Soluble Fraction of Perilla frutescens against Human Skin Melanoma Cells -
한국산 생약으로부터 항암물질의 개발 (제 5 보) - 소엽의 부탄올 가용분획이 인체피부흑색종 세포에 미치는 세포독성작용 -
- Lee, Ki-Nam (Department of Preventive Medicine, School of Oriental Medicine) ;
- Shin, Heuk-Ho (Department of Preventive Medicine, School of Oriental Medicine) ;
- Han, Du-Seok (Department of Oral Anatomy, School of Dentistry) ;
- Kim, Young-Ok (Department of Chemistry, College of Natural Sciences, Wonkwang University) ;
- Choi, Kyw-Eun (Department of Chemistry, College of Natural Sciences, Wonkwang University) ;
- Kwag, Jung-Suk (Department of Dental Hygenic, Mokpo Junior College) ;
- Baek, Seung-Hwa (Department of Chemistry, College of Natural Sciences, Wonkwang University)
- 이기남 (원광대학교 한의과대학) ;
- 신혁호 (원광대학교 한의과대학) ;
- 한두석 (치과대학 구강해부학교실) ;
- 김영옥 (자연과학대학 화학과) ;
- 최규은 (자연과학대학 화학과) ;
- 곽정숙 (목포전문대학 치위생과) ;
- 백승화 (자연과학대학 화학과)
- Published : 1997.12.30
Abstract
This Study was carried out develop antitumor effect of the n-butanol soluble of fraction of Perilla frutescens on human skin melanoma cells. The antitumor activity of various fractions obtained form n-butanol soluble fraction of Perilla frutescens was evaluated in human skin melanoma cells. The antitumor activity of the n-butanol soluble fraction in human skin melanoma cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, neutral red (NR) assay and sulforhordamine B protein (SRB) assay of colorimetic assay methods. The light microscopic study was carried out to observe morphological changes of cultured human skin melanoma cells. These results were obtained follows; The fractions 5 and 6 of the n-butanol soluble fraction of P frutescens were shown significant antitumor activities. The number of human skin melanoma cells were decreased and tend to form cell cluster by treatment with actions 5 and 7 of the n-butanol soluble fraction of P. frutescens. The fraction 6 of the the n-butanol soluble fraction showed the highest antitumor activity on P. frutescens. These results suggest that the fraction 6 of the n-butanol soluble fraction of P. frutescens may be a valuable choice for the studies on the treatment of human skin tumors.