Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
권호 표지

Cytotoxic Evaluation of the Ethyl Acetate Soluble Fraction of Perilla frutescens

소엽 Ethyl acetate 분획의 세포독성 평가

  • Kim Sung-Eun (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Chun Hyun-Ja (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kim Il-Kwang (Department of Chemistry, College of Natural Sciences, Wonkwang University) ;
  • Han Du-Seok (Department of Oral Anatomy, School of Dentistry, Wonkwang University) ;
  • Lee Hyun-Ok (Department of Oral Hygiene, Wonkwang Health Science) ;
  • Ahn Jong-Woong (Korea Institute of Chemical Research, Natural Products Research Team) ;
  • Lee Mee-Hee (Osio Oriental Medicine) ;
  • Baek Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 김성은 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 천현자 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 김일광 (원광대학교 자연과학대학 화학과) ;
  • 한두석 (원광대학교 치과대학 구강해부학교실) ;
  • 이현옥 (원광보건대학 치위생과) ;
  • 안종웅 (한국화학연구소 화학물질 연구단) ;
  • 이미희 (오시오 한의원) ;
  • 백승화 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2001.12.29
Download PDF
⟨ Previous Next ⟩

Abstract

The Cytotoxic activity of the ethyl acetate soluble fraction of Perilla frutescens on human oral epithelioid carcinoma cell lines was evaluated by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl-2H-tetrazalium bromide (MTT) colorimetric method. The light microscopic study was carried out to observe the morphological changes of cultured human oral epitheloid carcinoma cell lines (KB) These results were obtained as follows; 1. subfraction 1 of the ethyl acetate soluble fraction of perilla frutescens was shown significant cytotoxic activity (p<0.001) at 10-2 mg/ml concentration, this concentration was decreased the cytotoxic activity of 70.3% . 2. The comparison of IC50 values of various subfractions in human oral epithelioid carcinoma cell lines was exhibited that their susceptibility to these subfractions decreased in the following order ; subfraction 5 > subfraction 4 > subfraction 3 > subfraction 2 > subfraction 1 by MTT assay. In light microscopy, the subfraction 1 of the ethyl acetate soluble fraction of Perilla frutescens showed the highest cytotoxic activity. These findings suggest that subfraction 1 possessed the most cytotoxic constituents.

Keywords

References

  1. 약초의 성분과 이용 문관심
  2. 원색임상본초학 신민교
  3. Handbook of Food Additivos v.II Furia, T.E.
  4. 천연물대사전 김재길
  5. Kor. J. Food Sci. Technol. v.23 Volatile components of Perilla folium Jang, H.J.;Park, J.Y.;Kim, Y.T.
  6. Biosic. Biotech. Biochem. v.56 Priming effects of ve getable juice on endogeneous production of tumor necrosis factors Yamazaki, M.;Ueda, H.;Fukuda, K.;Okamoto, M.;Yui, S.
  7. Biosi. Biochem. v.56 Inhibition of perilla juice of tumor necrosis factor prodution Yamazak, I.M.;Ueda, H.;Du, D.
  8. 한국영양식량학회지 v.21 녹황색 채소류의 돌연변이유발 억제 및 AZ-521 위암세포의 성장 저해효과 박건영;이경임;이숙희
  9. 한국영양식량학회지 v.21 아플라톡신 B1과 4-NQO에 대한 녹황색 채소류의 항돌연변이 효과 이경임;박건영;이숙희
  10. 한국 생약학회지 v.25 소엽의 세포독성 및 항암작용에 관한 연구 한두석;정병호;유현경;김영옥;백승화
  11. 대한구강해부학회지 v.18 소엽의 메탄올 분획이 피부암세포에 미치는 항암효과 한두석;유현경;백승화
  12. 원광대학교 석사학위논문 소엽으로부터 부탄올 분획의 분리와 항암활성 최규은
  13. J. Immunol. Methods v.65 Repid colorimetric assays for celluar growth and survival: application of proliferation and cytotoxicity assays Mosmann, T.