• Title/Summary/Keyword: pretreatments

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ESR-based Identification of Radiation-Induced Free Radicals in Gamma-Irradiated Basil and Clove Using Different Sample Pre-Treatments (감마선 조사된 바질과 정향의 전처리방법에 따른 ESR Spectra 판별 특성)

  • Kwak, Ji-Young;Ahn, Jae-Jun;Akram, Kashif;Kwon, Joong-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.10
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    • pp.1454-1459
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    • 2012
  • An improved detection of radiation-induced paramagnetic faults was developed to identify the irradiation status of basil and clove. The effectiveness of different sample pretreatments, including freeze-drying (FD), oven-drying (OD), alcoholic-extraction (AE), and water-washing and alcoholic-extraction (WAE), were examined. All non-irradiated samples showed a single central signal ($g_0$=2.006), whereas radicals representing two additional side peaks ($g_1$=2.023 and $g_2$=1.986) with a mutual distance of 6 mT were detected in the irradiated samples. AE and WAE produced the best results for irradiated clove in terms of intensities of radiation-specific ESR signals and their ratios to the central signal. However, FD provided the highest intensities of radiation-specific ESR signals for basil, whereas their ratios to the major signal were better in the cases of AE and WAE. Signal noise, particularly due to $Mn^{2+}$ signals, was observed, whereas it decreased in AE and WAE pretreatments. Based on our results, AE and WAE can improve the detection conditions for radiation-specific ESR signals in irradiated samples.

A Study on the pretreatment of Activated Sludge for Bio-hydrogen Production process (생물학적 수소생산 공정 개발을 위한 오니 슬러지 전처리에 대한 연구)

  • Kim Dong Kkun;Kim Ji Seong;Kim Ho Il;Lee Yu Na;Pak Dae Won
    • 한국생물공학회:학술대회논문집
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    • 2004.07a
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    • pp.21-33
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    • 2004
  • In this study, Anaerobic sewage sludge in a batch reactor operating at $35^{\circ}C$ was used as the seed to investigate the effect of pretreatments of waste activated sludge and to evaluate its hydrogen production potential by anaerobic fermentation. Various pretreatments including physical, chemical and biological means were conducted to utilize for substrate. As a result, SCODcr of alkali and mechanical treatment was 15 and 12 times enhanced, compared with a supernatant of activated sludge. And SCODcr was 2 time increase after re-treatment with biological hydrolysis. Those were shown that sequential hybridized treatment of sludge by chemical & biological methods is most efficient process for sludge treatment. The pre-treatment activated sludge was tested to conform hydrogen production potential in batch experiments. When buffer solution was added to the activated sludge, hydrogen production potential increased as compare with no addition.

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Pretreatment of Cane Molasses for Production of Bacterial Cellulose and Its Physico-Chemical Properties (미생물 셀룰로오스 생산을 위한 당밀의 전처리 및 생산된 셀룰로오스의 물리화학적 특성)

  • Jung, Ho-Il;Jeong, Jin-Ha;Jeon, Young-Dong;Lee, Na-Ri;Park, Ki-Hyun;Kim, Yong-Gyun;Park, Geun-Tae;Son, Hong-Joo
    • Journal of Life Science
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    • v.19 no.10
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    • pp.1432-1437
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    • 2009
  • The aim of this study is to investigate cane molasses pretreatments for the production of cellulose by Acetobacter sp. V6, which has excellent bacterial cellulose (BC) producing capacity in the shaking culture. Among pretreatments of cane molasses, 1% (w/v) tricalcium phosphate (TP) treatment was more efficient in BC production. The physico-chemical properties of BCs that were produced in static and shaking cultures were also investigated. Although BC had an emulsifying ability, its emulsion stability was low. Water holding capacity (WHC) of BC was high; the WHC of BC produced in static culture was 14 times higher than that of $\alpha$-cellulose. In addition, the viscosity of BC was higher than that of $\alpha$-cellulose. Composition analysis by FT-IR showed no difference in composition between BC and plant cellulose. In the crystallinity analysis by XRD, all BC samples showed crystallinity. All BC samples showed reticulated structures consisting of ultrafine cellulose fibriles. Microfibriles of cellulose from static culture were especially more compact than those of cellulose from shaking culture.

Properties of Porphyran and Hemicellulose extracted with Different extract Solutions and Enzymatic Pretreatments from Porphyra (추출 용매 및 효소 전처리 방법에 따른 포피란과 헤미셀루로오즈의 특성)

  • AN, Se-Ra;KOO, Jae-Geun
    • Journal of Fisheries and Marine Sciences Education
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    • v.29 no.1
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    • pp.108-117
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    • 2017
  • Laver, Porphyra, is distinctive for its high content of proteins and polysaccharides such as porphyran and hemicellulose. The chemical properties of the polysaccharides extracted with different extraction methods such as hot water, dilute acid(pH 4.0) or alkali solution(2N NaOH) were examined to investigate the suitable extraction conditions for porphyran and hemicellulose from laver. For porphyran extraction, dilute acid solution was more preferable to hot water and alkali solution because of its higher 3,6-anhydrogalactose content and lower protein content. However, alkali solution was more suitable to extract the hemicellulose because of higher mannose content indicating the extraction of mannan. To decrease contamination of the polysaccharides with protein, the dried lavers were pretreated with enzymes (Protamex, Flavourzyme, Alcalase, Viscozyme) before hot water extraction. All enzyme pretreatments increased the yield of polysaccharides by compared with control (enzyme unpretreated) and Flavourzyme pretreatment was most effective to decrease protein contamination in the polysaccharide. All viscosities of porphyran solutions pretreated by enzymes were lower compared to the control porphyran solution and showed pseudoplastic behavior with yield stress. In case of alkali extraction of residues obtained after enzyme hydrolysis and hot water extraction, protease pretreatment increased the mannose contents in the polysaccharide while the xylose content was increased by Viscozyme pretreatment.

Effects of Pretreatments and Storage Conditions on Quality and Vase Life of Cut 'Red Sandra' Rose (전처리와 저장조건이 절화장미 'Red Sandra'의 품질과 수명에 미치는 영향)

  • Bang, Chang-Seok;Song, Cheon-Young;Lee, Jong-Suk;Huh, Kun-Yang;Song, Jeong-Seob
    • Horticultural Science & Technology
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    • v.17 no.6
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    • pp.762-764
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    • 1999
  • This experiment was conducted to investigate the effect of pretreatments and storage conditions on quality and vase life of cut 'Red Sandra' rose (Rosa hybrida L.). 'Red Sandra' rose flowers were harvested and pulsed with distilled water, 0.2% RVB or 200ppm aluminum sulfate+3% sucrose+50ppm $AgNO_3$+0.1mM ethionine for 22 hours and then stored in wet or dry condition for 12, 24, 48 hours at low or room temperature. Pulsing with aluminum sulfate+sucrose+$AgNO_3$+ethionine delayed bent-neck, increased solution absorption and flower diameter, and prolonged vase life rather than pulsing with distilled water or RVB. Storage in wet condition or low temperature also delayed bent-neck, increased solution absorption and flower diameter, and prolonged vase life compared with storage in dry or room temperature condition. As the storage hours prolonged, solution absorption, flower diameter, and vase life were decreased especially pulsing with distilled water. Pulsing with aluminum sulfate+sucrose+$AgNO_3$+ethionine prolonged vase life compared to pulsing with distilled water or RVB regardless of storage conditions and shipping hours.

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Neuropretective effect of Kupunggibodan, Gamisamul-tang and Whangryunhaedok-tang on the ischemia-induced learning and memory deficits by MCAO in the rats (중풍 한방처방전의 효능비교 연구 ; 황련해독탕, 거풍지보단, 가미사물탕이 국소 전뇌허혈에 의한 학습과 기억에 미치는 효과)

  • Lee Bom-Bi;Chung Jin-Yong;Kim Sun-Yeou;Kim Ho-Cheol;Kwon Youn-Jun;Hahm Dae-Hyun;Lee Hae-Jeong;Shim In-Sup
    • Korean Journal of Acupuncture
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    • v.19 no.2
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    • pp.63-78
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    • 2002
  • Kupunggibodan(KU), Gamisamul-tang(GA) and Whangryunhaedok-tang(WH) are clinically the most popular prescriptions as an herbal medicine in the treatment of ischemia. In order to compare and evaluate their protective effects on the ischema-induced cognitive deficits by middle cerebral artery occlusion (MCAO), we examined its ability to improve ischemia-induced cell loss and impairements of learning and memory in the Morris water maze and eight-arm radial arm maze. Focal cerebral ischemia produced a marked cell loss, decrease in acetylcholinesterase(AchE) reactivity in the hippocampus, and learning and memory deficits in two behavioral tasks. Pretreatment with WH (100 mg/kg, p.o.) produced a substantial increase in acquisition in the Morris water maze. Pretreatment with KU increased the perfomance of the resention test in the Morris water maze. WH, KU and GA caused a significant improvement in choice accuracy in radial arm maze test. WH was superior to KU and GA in perfomance of the radial arm maze test. Consistent with behavioral data, staining with cresyl violet showed that pretreatments with WH, but not KU and GA significantly recovered the ischemia-induced cell loss in the hippcampal CA1 area. In addition, pretreatments with WH and KU recovered the ischemia-induced reduction of AchE reactivity in the hippocampal CA1 area. These results demonstrated that KU, GA and WH have protective effects against ischimea-induced learning and memory impairments and that the efficacy was the order of WH>KU>GA in tratment of ischemia induced memory deficits. The present studies provide an evidence of KU, GA and WH as putative treatment of vascular dementia. Supported by a fund from the Ministry of Health and Welfare(HMP-00-OO-04-0004), and the Brain Korea 21 Project from Korean Ministry of Education, Korea.

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Extraction Yield and Quality Attributes of Agar from Imported Seaweeds According to Various Pretreatments (수입원조(輸入原藻)의 전처리(前處理)과정에 따른 한천(寒天)의 수율(輸率) 및 품질특성(品質特性))

  • Cho, Han-Ok;Chung, Man-Jai;Lee, Su-Rae
    • Korean Journal of Food Science and Technology
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    • v.7 no.3
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    • pp.115-119
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    • 1975
  • Imported seaweeds Gracilaria sp. and Gelidium sp. from the Philippines and Gracilaria sp. from Brazil were subjected to the examination of yield and quality of agar according to various pretreatment conditions. As pretreatment condition for alkali-treated Gracilaria sp. from the Philippines, $0.05{\sim}0.1%$ concentration of sulfuric acid was proper to obtain the higher yield of agar for which a reverse proportionality was shown between the acid concentration and the strength of extraction condition. Highest yield from Gelidium sp. from the Philippines was obtained by pretreating with 5% sodium hydroxide at $90^{\circ}C$ for one hour, followed by 0.1% sulfuric acid treatment. Gracilaria sp. from Brazil gave the highest yield of agar by pretreatments with 1% sodium hydroxide followed by 0.05% sulfuric acid, both at room temperature for one hour. Acid treatment of alkali-treated Gracilaria sp. from the Philippines brought about the decrease of total nitrogen, total sulfur and crude ash, the increase of jelly strength and no marked change in gelation point and gelation ability. In general, acid-treatment conditions to give rise to high yield of agar improved the quality of product.

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Extraction Yield and Quality Attributes of Agar from Domestic Seaweeds According to Various Pretreatments (국산원조(國産原藻)의 전처리(前處理)과정에 따른 한천(寒天)의 수율(收率) 및 품질특성(品質特性))

  • Lee, Su-Rae;Cho, Han-Ok;Park, Sang-Ki
    • Korean Journal of Food Science and Technology
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    • v.7 no.3
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    • pp.109-114
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    • 1975
  • Domestic seaweeds Gracilaria verrucosa collected from coastal areas of Namhae, Wando and Yeosoo, Korea were subjected to the examination of yield and quality of agar prepared according to various pretreatment conditions. In alkali treatment at high temperature, higher alkali concentration for one-hour period at $90^{\circ}C$ gave rise to higher yield of agar. In acid treatment, higher yield was obtained by higher acid concentration and longer treating period. Alkali treatment at room temperature gave rise to a slightly decreased yield at higher alkali concentration and longer treating time. Total nitrogen and crude ash of agar samples were greatly decreased by pretreatments. Jelly strength, gelation ability, gelation point and viscosity of agar samples tended to increase as the alkali-treating condition of seaweed became stronger. It was shown that sulfur content of agar had a high negative correlation with jelly strength of its gel. Various alkali treatments of seaweed at room temperature showed no marked difference in agar quality and did not exhibit any good effect comparable to alkali treatment at high temperature.

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Organic Acid Composition and Flavor Characteristics of Lactic Acid Fermented Cereal Beverages

  • Yi, Do-Youn;Kim, Gi-Myung;Lee, Ki-Young
    • Journal of Microbiology and Biotechnology
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    • v.3 no.2
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    • pp.129-133
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    • 1993
  • The effect of different compositions of organic acids on the flavor profile of 10% sugar solution was investigated by the response surface methodology, and the results were used to evaluate the flavor characteristics of lactic acid fermented cereal beverages. A mixture of extruded rice flour (10%) and soymilk (7.8% dry matter) was fermented with Leuconostoc mesenteroides (Sikhae). Depending on the substrate pretreatments, for example, the malt or amylase digestion and the proteolytic enzyme hydrolysis, the sugar and organic acid composition of the product varied. The organic acid composition of the fermented beverages was in the ranges of 0.44-0.55% lactic acid, 0.05-0.09% acetic acid and 0.07-0.09% citric acid, while that of commercial apple juice was 1.59% malic acid and 0.49% acetic acid. The flavor profiles of fermented beverages added with 10% sucrose were compared to those of apple juice and a model mixture containing 0.48% citric acid, 0.39% lactic acid and 0.12% acetic acid in 10% sugar solution. The QDA diagram of fermented beverages approached to that of apple juice, when the substrate was digested by amylase but not by protease.

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Surface Modification of PET Irradiated by Ultra-Violet (Part II) - Transformation of moisture properties and physical Properties - (UV조사를 통한 PET의 표면개질(제2보) -수분특성 및 물리적 특성 변화-)

  • Choi Hae Young;Lee Jung Soon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.29 no.5 s.142
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    • pp.617-625
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    • 2005
  • The purpose of this study was to develop functional and environment-friendly polyester fabric by irradiating Ultra-Violet, which was produced by the low-pressure mercury lamp. UV irradiation was conducted with various treatment times and distances. Also, pretreatments of solvents and photoinitiator were used to improve the surface modification effects. The effects of UV irradiation on the moisture regain were found to increase gradually with increase of UV treatment time and decrease of treatment distance. Compared the effects of UVC and UVA, UVC was more effective than UVA. Moisture regain and wicking of PET was increased after UV treatment in our experimental condition. And owing to increasing of moisture regain, static charge was decreased. Pretreatment of solvents such as methanol, ethyl ether and addition of photoinitiator such as Benzophenone accelerated surface modification. The moisture regain was increased but wicking was decreased with pretreatment and addition of photoinitiatior. Therefore it is considered as inappropriate f3r clothing because of wicking effects. Yellowness, tensile strength and elasticity slightly decreased after UV irradiation.