• Title/Summary/Keyword: pretreated method

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Effects of High-Hydrostatic Pressure on Ginsenoside Concentrations in Korean Red Ginseng

  • Kim, Sun-Ok;Park, Chan-Woong;Moon, Sang-Young;Lee, Hyun-A;Kim, Byong-Ki;Lee, Dong-Un;Lee, Jae-Ho;Park, Ji-Yong
    • Food Science and Biotechnology
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    • v.16 no.5
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    • pp.848-853
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    • 2007
  • The effects of high-hydrostatic pressure (HHP) on the ginsenoside concentration in Korean red ginseng were investigated. HHP-pretreated Korean red ginseng samples were compared to samples produced by a conventional method. Six-year-old Korean fresh ginseng (Panax ginseng C.A. Meyer) samples were vacuum-packaged in polyethylene film and treated at room temperature for 1 min with HHP (200-600 MPa) and steamed at $98^{\circ}C$ for 3 hr. Major ginsenosides of red ginseng were analyzed by HPLC. HHP-pretreated red ginseng showed a 45% higher level of total major ginsenosides than conventionally prepared red ginseng. The levels of 4 protopanaxadiol-type ginsenosides increased 34-43% and the levels of 5 protopanaxatriol-type ginsenosides increased 45-66%. Scanning electron microscopy and electrical conductivity spectrum analysis showed that HHP pretreatment damaged ginseng plant cells and increased extraction efficiencies of ginsenosides from red ginseng products.

The investigation of conservation methods and destruction regions of papers under the various temperature and humidity changes (온.습도 변화에 따른 양지의 손상원인 및 보존방안 연구)

  • Lee, Hye-Yun;Chung, Yong-Jae;Lee, Kyu-Sik;Han, Sung-Hee
    • 보존과학연구
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    • s.21
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    • pp.145-175
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    • 2000
  • To examine the method for preservation of papers, 9 kinds of papers were purchased and pretreated for 6 months with various artificial conditions which were controlled as 3 temperature classes($20^{\circ}C$, $80^{\circ}C$, $-20^{\circ}C$&$20^{\circ}C$) and 4 humidity classes(40%, 60%, 80%, 100%).Whiteness, acidity and folding endurance were measured to examine the state of papers pretreated under each condition. From the resultexamining the state, all the papers were best condition at low temperature($20^{\circ}C$) and low humidity(40%) and damaged at high temperature($80^{\circ}C$) and high humidity(100%).The values of whiteness, acidity, and folding endurance were measure din relation with deterioration of papers. According to damage of papers caused by the chemical agent, papers became to be acidic, increased color difference and reduced the force of folding endurance. The effect of change of temperature($-20^{\circ}C$&$20^{\circ}C$) on degradation of papers was not well acquired. Because the period of investigation was not long enough to acquire the data. So the more studies are needed to find the cause of damage of papers.

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Characteristics of Enzymatic Hydrolysis of Sodium Hydroxide pretreated Suwon Poplar (NaOH 전처리된 현사시나무의 효소가수분해 특성)

  • 박영기;오정수
    • Journal of Korea Foresty Energy
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    • v.20 no.2
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    • pp.20-27
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    • 2001
  • An effective method for production of glucose was developed using enzymatic hydrolysis of Suwon poplar by the cellulase. Enzymatic hydrolysis of wood is the reaction to produce glucose from wood using enzyme which derives from microorganism. Glucose can be transferred easily to ethanol by fermentation. Ethanol is the starting material for producing acetone, butanol, citric acid and lactic acid. The mechanism of the enzymatic hydrolysis of cellulose are reasonably explained in terms of the sequential action of three different types of enzymes, endo-cellulase, ex-cellulase, and $\beta$ -glucosidase. The goal of this work was to investigate the cellulose hydrolysis pretreated polar with various concentration NaOH, the crystallinity of cellulose, lignin contents and the degree of hydrolysis.

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Effects of Osmotic Dehydration on Drying Characteristics of Kiwifruits (키위의 건조특성에 미치는 삼투처리의 영향)

  • 윤광섭;홍주헌
    • Food Science and Preservation
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    • v.6 no.3
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    • pp.319-323
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    • 1999
  • This study was conducted to minimize the deterioration of dried kiwifruit quality. Osmotic dehydration was carried out as pretreatment before drying. After the kiwifruits were pretreated under optimized osmotic dehydration conditions, they were dried by three drying methods(hot air drying, vacuum drying, freeze drying). Hot air drying and vacuum drying were superior to freeze drying in the drying speed. But vacuum and freeze drying preserved more vitamin C than hot air drying. Also, osmotic dehydrated kiwifruit kept better quality than nontreated kiwifruit. Diffusion coefficient which describes moisture transfer, was high in drying process pretreated with osmosis. The changes of vitamin-C followed the second-order reaction rate equation with high RE, respectively.

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Effect of Low Dose Mutagens on Adoptive Response and Plasma Membrane Glycoconjugates in Sarcoma 180 Cells

  • Lee, Jong Hwa;Choi, Eun Sili;Oh, Kyu Seon;Lee, Dong Wook;Chang, Jeong Hyun;Um, Kyung Il
    • Animal cells and systems
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    • v.4 no.3
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    • pp.293-297
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    • 2000
  • The present investigation was performed to elucidate the effect of pretreatment with low dose ultraviolet radiation (UV) and ethyl methanesulfonate (EMS) on cell survival by trypan blue dye exclusion method and plasma membrane glycoconjugates by lectin-cytochemistry in sarcoma 180 (S180) cells. Pretreatment with 2 J/$m^2$ UV or 2 mM EMS increased the percentage of survival of cells subsequently treated with high dose UV (10 or 20 J/$m^2$) or EMS (10 or 20 mM), respectively. Staining intensity of concanavalin A (Con A) of the cells pretreated with 2J/$m^2$ UV or 2 mM EMS and subsequently treated with 10 or 20 mM EMS was stronger than that of the cellstreated with 10 or 20 mM EMS. These results suggest that there is an adaptive response on cell survival to EMS or UV in S180 cells. And the results show a change in mannose-containing glycoconjugates of plasma membrane in S180 cells pretreated with EMS or UV and subsequently treated with EMS.

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Improving the Dyeability of Gelatin Pretreated Cotton Fabrics Dyeing with Cochineal in Ethanol-Water Mixture (젤라틴 전처리 면직물의 에탄올-물 혼합용매에 의한 코치닐 염색성 향상)

  • Ha, Su Young;Jang, Jeong Dae
    • Textile Coloration and Finishing
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    • v.31 no.3
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    • pp.127-134
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    • 2019
  • Cotton fabrics were treated with gelatin to improved their dyeability, color strength toward cochineal dye. Gelatin were used as the protein. Gelatin is containing a large number of hydrophilic groups. Pad-dry-cure method was used for the treatment process(10g/L concentration). The scanning electron micrograph showed the gelatin was deposited on the surface of cotton. Pretreated fabrics were mordanted with 10%(owf) alum. Then the fabrics were dyed with cochineal. Compared with original cotton fabric the K/S value with cochineal dyes was significantly improved on gelatin modified cotton. Treating cotton with 10g/L concentration gelatin offered higher cochineal adsorption. The dyeability of pH 4 yielded the highest color strength. In dyebaths of a ratio of ethanol and water such as; 10:0, 9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8, 1:9, 0:10, fabrics were dyed. The ratio of ethanol and water had powerful effects on solution polarity. Cochineal dye uptake showed maximum value, when the proportion of ethanol and water was 9:1. Dyeing at increased temperatures and with increased time resulted in higher dye uptake and reddish-purple color(5RP). The washing fastness was 1-2grade, and the fastness to light was 2-3grade.

Inhibitory Effect of Phorbol 12-Myristate 13-Acetate on NO Production Induced by Interleukin-1 beta in Aortic Vascular Smooth Muscle Cells of Rats (혈관평활근세포에서 Phorbol 12-Myristate 13-Acetate의 전처리가 Interleukin-1β에 의한 Nitrite생성에 미치는 영향)

  • 윤병헌;김인겸;박태규;김중영
    • Journal of Life Science
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    • v.13 no.4
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    • pp.441-447
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    • 2003
  • To examine the role of protein kinase C (PKC) in regulation of interleukin-1 beta (IL-1$\beta$)-induced iNOS expression, IL-1$\beta$-induced nitrite production was observed in cultured vascular smooth muscle (VSM) cells pretreated with phorbol 12-myristate 13-acetate (PMA) and phorbol 12,13-butyrate (PDB) as PKC activator; 4$\alpha$-phorbol-didecanoate (PDD) as PKC non-activator. Nitrite production induced by IL-1$\beta$ was increased by the presence of increasing concentration of PMA ranging from 2 to 200 nM. However, in VSM cells pretreated with PMA and PDB, IL-1$\beta$-induced $NO_2$ production was decreased in proportion to the duration of pretreatment, and most significantly decreased in pretreatment time of 24 hours. Using RT-PCR method, the expression of iNOS mRNA induced by IL-1$\beta$ was decreased in VSM cells pretreated with PMA 200 nM for 24 hours. These results suggest that decrease in IL-I$\beta$-induced nitrite production by the pretreatment of PMA result from inhibition of iNOS expression and the inhibition related to PMA-induced PKC down-regulation.

A Preliminary Investigation on Pozzolanic Activity of Dredged Sea Soil (소성 준설토의 포졸란 반응성에 대한 기초 연구)

  • Kim, Ji-Hyun;Moon, Hoon;Chung, Chul-Woo;Lee, Jae-Yong
    • Journal of the Korea Institute of Building Construction
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    • v.14 no.6
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    • pp.531-536
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    • 2014
  • Recently, the amounts of dredge sea soil in south Korea have been increasing because of various maintenance works at harbors and rivers. Dredged sea soil contains various contaminants. Hence, prior to recycling the dredged sea soil, the various contaminants should be removed to prevent a secondary contamination due to the leaching of hazardous chemicals. Pretreated dredged sea soil can be buried under the ground or used for land reclamation. In this study, however, pretreated dredged sea soil was used to investigate the level of pozzolanic activity. The properties of pretreated dredged sea soil were investigated, the method for heat treatment was determined, and the compressive strength of mortar using dredged sea soil was examined. According to the XRF result, the main components of dredged sea soil were $SiO_2$ of over 55%, and $Al_2O_3$ and $SO_3$ of some amounts. Results from XRD and TG/DTA showed that pretreated dredged sea soil can be used as a pozzolanic material. When dredged sea soil was thermally treated for 90 min at $550^{\circ}C$, a compressive strength result was similar to that of control mortar.

Maximizing biogas production by pretreatment and by optimizing the mixture ratio of co-digestion with organic wastes

  • Lee, Beom;Park, Jun-Gyu;Shin, Won-Beom;Kim, Beom-Soo;Byun, Byoung-su;Jun, Hang-Bae
    • Environmental Engineering Research
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    • v.24 no.4
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    • pp.662-669
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    • 2019
  • Anaerobic digestion is a popular sewage sludge (Ss) treatment method as it provides significant pollution control and energy recovery. However, the low C/N ratio and poor biodegradability of Ss necessitate pretreatment methods that improve solubilization under anaerobic conditions in addition to anaerobic co-digestion with other substrates to improve the process efficiency. In this study, three pretreatment methods, namely microwave irradiation, ultrasonication, and heat treatment, were investigated, and the corresponding improvement in methane production was assessed. Additionally, the simplex centroid design method was utilized to determine the optimum mixture ratio of food waste (Fw), livestock manure (Lm), and Ss for maximum methane yield. Microwave irradiation at 700 W for 6 min yielded the highest biodegradability (62.0%), solubilization efficiency (59.7%), and methane production (329 mL/g VS). The optimum mixture ratio following pretreatment was 61.3% pretreated Ss, 28.6% Fw, and 10.1% Lm. The optimum mixture ratio without pretreatment was 33.6% un-pretreated Ss, 46.0% Fw, and 20.4% Lm. These results indicate that the choice of pretreatment method plays an important role in efficient anaerobic digestion and can be applied in operational plants to enhance methane production. Co-digestion of Ss with Fw and Lm was also beneficial.

The Action of Panaquilon on the Isolated Clam Heart (Panaquilon의 적출조개 심장운동에 대한 작용)

  • Song, Suk-Kyu
    • The Korean Journal of Pharmacology
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    • v.6 no.1
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    • pp.9-13
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    • 1970
  • The effect of Panaquilon (Partial Component of Panax ginseng), on the isolated clam heart, the motility of the isolated clam heart suspended in physiological solution for clam heart was recorded by the Magnus Method. 1. The concentration of $5{\times}10^{-6}$ of Panaquilon produced cardiac arrest in the diastolic state, which resumed the normal state slowly after changing the physiological solution for clam heart. 2. Cardiac arresting action of Panaquilon was antagonistic when pretreated with Serotonin.

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