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http://dx.doi.org/10.5352/JLS.2003.13.4.441

Inhibitory Effect of Phorbol 12-Myristate 13-Acetate on NO Production Induced by Interleukin-1 beta in Aortic Vascular Smooth Muscle Cells of Rats  

윤병헌 (경북대학교 의과대학 약리학교실)
김인겸 (경북대학교 의과대학 약리학교실)
박태규 (경북대학교 의과대학 약리학교실)
김중영 (경북대학교 의과대학 약리학교실)
Publication Information
Journal of Life Science / v.13, no.4, 2003 , pp. 441-447 More about this Journal
Abstract
To examine the role of protein kinase C (PKC) in regulation of interleukin-1 beta (IL-1$\beta$)-induced iNOS expression, IL-1$\beta$-induced nitrite production was observed in cultured vascular smooth muscle (VSM) cells pretreated with phorbol 12-myristate 13-acetate (PMA) and phorbol 12,13-butyrate (PDB) as PKC activator; 4$\alpha$-phorbol-didecanoate (PDD) as PKC non-activator. Nitrite production induced by IL-1$\beta$ was increased by the presence of increasing concentration of PMA ranging from 2 to 200 nM. However, in VSM cells pretreated with PMA and PDB, IL-1$\beta$-induced $NO_2$ production was decreased in proportion to the duration of pretreatment, and most significantly decreased in pretreatment time of 24 hours. Using RT-PCR method, the expression of iNOS mRNA induced by IL-1$\beta$ was decreased in VSM cells pretreated with PMA 200 nM for 24 hours. These results suggest that decrease in IL-I$\beta$-induced nitrite production by the pretreatment of PMA result from inhibition of iNOS expression and the inhibition related to PMA-induced PKC down-regulation.
Keywords
Smooth Muscle; Phorbol 12-Myristate 13-Acetate; Nitrate; Interleukin-1${\beta}$;
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