• The Korean Journal of Pesticide Science
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• v.3 no.2
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• pp.81-85
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• 1999

Concentration change of soil-applied pretilachlor (2-chloro-2',6'-diethyl- N-2-propoxyethyl)-actanilide) was investigated under upland condition with various soil moisture contents ranging from 50 to 80%(water content by weight). Following pretilachlor from each soil solution was extracted by centrifugation using double tubes, its concentration was determined by HPLC. Pretilachlor concentration in the soil solutions were almost the same under various soil moisture conditions. However, the total amount of pretilachlor increased as the soil moisture content increased. With increasing soil moisture content, the bioactivity of soil-applied pretilachlor on inhibiting the growth of Echinochloa ultilis Ohwi et Yabuno and the absorption of $^{14}C$-pretilachlor in its plants were also enhanced. Our results demonstrate that the absorption of pretilachlor in plants varies with soil moisture content and thus the bioactivity of soil-applied pretilachlor on inhibiting plant growth is different under various soil moisture conditions at the same dosage based on air-dried weight.

• Environmental Analysis Health and Toxicology
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• v.19 no.1
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• pp.93-100
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• 2004

Pretilachlor [2-chloro-N -(2, 6-diethylphenyl)-N-(2-propoxyethyl) acetamide, $C_{17}$H$_{26}$ClNo$_2$, M.W.=311.9, CAS No.51218-49-6]는 제초제의 일종으로 본 연구에서는 박테리아 복귀 돌연변이 시험과 포유동물 세포를 이용한 염색체 이상 시험 및 마우스를 이용한 in vivo소핵 시험을 수행하여 pretilachlor의 유전독성을 평가하였다. 박테리아 복귀 돌연변이 시험에서 pretilachlor는 Salmonella typhimurium TA98, TA 100, TA1535, TA1537 균주의 대사 활성계 존재 및 부재시 313-5,000$\mu\textrm{g}$/plate의 범위에서 농도 의존적인 돌연변이 율의 증가를 관찰할 수 없었다. 또한 포유동물 세포인 Chinese hamster lung (CHL) fibroblast를 이용한 염색체 이상 시험에서 pretilachlor는 대사 활성계 존재 및 부재시 1.56-6.24$\mu\textrm{g}$/mL의 농도에서 clastogenicity를 보이지 않았고, 137.5-550.1 mg/kg의 pretilachlor를 복강 주사한 마우스의 골수세포를 이용한 in vivo소핵 시험의 결과에서도 통계적으로 유의한 소핵 유발능을 관찰할 수 없었다었다

• Korean Journal of Weed Science
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• v.6 no.1
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• pp.59-66
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• 1986

This experiment was conducted with pot trial to evaluate effects of pretilachlor [2-chloro-2', 6'-diethyl-N(n-propoxythyl) acetanilide], pyrazoxyfen [ 1,3-dimethyl-4-(2,4-dichloro benzoyl)-5-phenacyloxy-pyrazole], pyrazolate (4-(2,4-dichloro benzoyl)-1,3-dimethyl-pyrazol-5-yl-p-toluenesulphonate] and their combinations on Echinochloa crew-galli. Herbicides were treated with different dosages under 3 cm water depth at 1st and 2nd leaf stages of E. crus-galli. E. crus-galli showed stunted symptom by treatment of pretilachlor and etiolation by treatment of pyrazoxyfen or pyrazolate after 4 days from treatment. Stunting and chlorosis degrees of E. crusgalli increased with high dosage of all herbicides at lst and 2nd leaf stage. Combination of pyrazoxyfen+pretilachlor (6+1.5)G or pyrazolate+pretilachlor (6+1.5)G showed positive synergistic effect of herbicidal action on E. crus-galli, therefore these combinations can control E. crux galli in paddy field with lower dosage than each single treatment of herbicides. Herbicidal effect of pyrazoxyfen on E. crus-galli was similar to pyrazolate in single treatment and combination with pretilachlor.

• Korean Journal of Weed Science
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• v.7 no.2
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• pp.179-185
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• 1987

Laboratory studies were conducted to evaluate safening effect of CGA 123'407 (4,6-dichloro-2-phenyl-pyrimidine) against pretilachlor, butachlor, and benthiocarb in rice plants. Butachlor and benthiocarb at 50 ppm or higher concentrations significantly inhibited rice growth and pretilachlor gradually inhibited growth of rice plants with increase in concentration starting from 0.5 ppm. CGA 123'407 at 0.1 ppm or higher concentrations was effective in reducing injury of rice plants when butachlor and benthiocarb were applied at rate of 10 ppm. Rice injury from pretilachlor at 10 ppm was also reduced by addition of CGA 123'407 at 0.25 ppm or higher concentrations Herbicidal activity of pretilachlor, butachlor, and benthiocarb was not affected by mixture treatments of CGA 123'407.

• Korean Journal of Weed Science
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• v.10 no.4
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• pp.328-337
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• 1990

The incividual and combined effects of the chloroacetanilide herbicide pretilachlor and of the safener fenclorim on the growth and selected physiological processes of rice (Oryza sativa L., var 'Lemont')were evaluated under greenhouse and laboratory conditions. Fenclorim applied at rates ranging from 50 to 300 g a.i./ha antagonized the injurious effects caused by 150 to 900 g a.i./ha of pretilachlor on 15-day old wet-sown rice grown under greenhouse conditions. When used rates of 150 g/ha or higher, fenclorim reversed completely the effects of all doses of pretilachlor on rice. When the two compounds were given simultaneously, fenclorim enhanced the uptake of $^{14}C$pretilachlor into rice leaf mesophyll protoplasts measured for 1 hr, indicating that competition for uptake at the protoplast level is not involved in the protective action of this safener. The safener-induced stimulation of pretilachlor uptake was particularly evident when fenclorim was used at concentrations of 10, 20 and $40{\mu}M$. Following 4 hr of incubation, individual treatments with pretilachlor inhibited the in vitro incorporation of radiolabeled precursors into proteins, DNA, and lipids of rice leaf protoplasts only when used at the high concentration of $100{\mu}M$M. Individual treatments with high concentrations (10 or $100{\mu}M$) of the safener fenclorim inhibited the incorporation of radiolabeled precursors into proteins and lipids of rice protoplasts, but had no DNA synthesis. The combined effects of pretilachlor and fenclorim on the incorporation of radiolabeled precursors into these macromolecules of isolated rice mesophyll protoplasts appeared to be additive or slightly synergistic rather than antagonistic. Fenclorim at $1{\mu}M$ antagonized the effects of pretilachlor on total lipids of rice leaf protoplasts. In addition, individual and combined treat-menu with pretilachlor and fenclorim influenced the incoroporation of$^{14}C$acetate into polar lipids, triglycerides and steryl esters of rice leaf protoplas causing a redistribution of carbon in these lipid fractions. However, these effects were not large enough to explain the herbicidal activity of pretilachlor or to account for the protective action of the safener fenclorim. Overall, the uesults of the present study idnicate that the safener fenclorim does not seem to protect rice against pretilachlor injury by antagonizing its effects on protein, DNA, or lipid syntheses.

• Korean Journal of Weed Science
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• v.6 no.2
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• pp.154-161
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• 1986

Since herbicide damage has been occurred in subirrigated rice seedbed in Korea, the use of crop safener is required to reduce crop injury. Field studies were conducted to evaluate safening effect of 1,8-naphthalic anhydride (NA) and CGA 123'407 (4, 6-dichloro-2-phenyl-pyrimidine) against four kinds of paddy herbicides applied to subirrigated vinyl-covered rice seedbed. NA as a seed dressing at 0.5% (W/W) was effective in reducing rice injury from butachlor, pretilachlor, chlormethoxynil and benthiocarb, and increased number of seedling established, plant height, and dry weight of rice seedlings, while growth inhibition and stand loss were observed at 1% NA. Pretilachlor slightly decreased number of seedlings established and inhibited growth of rice seedlings. But pre-mix combination of pretilachlor (2%) and CGA 123'407 (0.5%) reduced rice injury caused by pretilachlor alone and increased number of seedlings established and dry weight of rice seedlings. The results indicate that NA and CGA 123'407 would permit the safe application of butachlor, pretilachlor, chlormethoxynil, and benthiocarb in subirrigated rice seedbed.

• Korean Journal of Weed Science
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• v.5 no.2
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• pp.164-168
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• 1985

In order to enhance herbicidal efficacy of amide and diazine herbicides, synergistic effects of pretilachlor and pyrazoxyfene or butachlor and pyrazolate on control of Cyperus serotinus were determined by isobole method. Interaction indices(I) between pretilachlor and pyrazoxyfene treated at 0 and 3rd leaf stage were 2.64 and 2.07 and hence showed synergistic effect for control of Cyperus serotinus. The points indicated Imax between pretilachlor and pyrazoxyfene were 0.7:1.3 g ai/a and 9.0:20.5 g ai/a at 0 and 3rd leaf stage, respectively. Combination of butachlor and pyrazolate showed synergistic effect(1=1.57) on control of Cyperus serorinus and point indicated Imax was 48.0:20.9 g ai/a.

• Applied Biological Chemistry
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• v.31 no.2
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• pp.200-204
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• 1988

This study was conducted to evaluate effect of soil texture, organic matter, temperature and water regime on degradation of pretilachlor (2-chloro-2',6'-diethyl-N-(n-propoxyethyl) acetanilide) in the soils. The period of residual activity in soil treated with pretilachlor at rate of 60g a.i./10a was about 50 days. Also, period of reaching GR 50 value on the barnyard grass was about $25{\sim}27$ days. Degradation rate of pretilachlor in the sandy clay loam soil which has higher contents of organic matter and clay was faster than in the sandy loam soil. When organic matter was added to the soil, the rate of decomposition was accelerated. The faster degradation occurred under the $30^{\circ}C$ soil temperature by comparison under the $20^{\circ}C$. The rate of degradation in the soil was faster under the flooded conditions than under the wetted conditions.

• Applied Biological Chemistry
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• v.30 no.4
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• pp.351-356
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• 1987

This study was conducted to investigate the absorption and translocation of pretilachlor [2-chloro-2, 6-diethyl-N-(n-propoxyethyl)-acetanilide] in plants and to evaluate the mobility in soil using the $^{14}C-or$ non-labelled compound in laboratory. Rice plant(Oryza sativa L.) was very tolerant to pretilachlor. Echinochloa crus-galli P. Beauv. was completely controlled by pretilachlor at 60g a.i./10a. At the 120g a.i./l0a, growth of Cyperus serotinus Rottb. and Sagittaria pygmaea Miq. was inhibited by 75% and 25%, respectively. The growth inhibition depended on absorbed amount of $^{14}C-pretilachlor$. The greatest concentration of $^{14}C$ was found in E. crus-galli, whereas the lowest was determined in rice plant. The rate of absorption and translocation in E. curs-galli was faster than in rice plan. Pretilachlor moved to 6cm deep in sandy clay loam, clay loam and loam soils, but to 10cm in sandy loam soil. In the soils herbicide-treated layer was found 0 to 2cm profile.

• Korean Journal of Weed Science
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• v.5 no.2
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• pp.155-163
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• 1985

Three pyrazole-herbicides, pyrazolate, pyrazoxyfene and benzophenap, were evaluated for their interaction in controlling barnyardgrass (Echinochloa crusgalli) with two chloroacetamide-herbicides, butachlor and pretilachlor. Percent inhibition of barnyardgrass growth by pyrazolate, pyrazoxyfene, and benzophenap was 44%, 64%, and 0%, respectively, when each was applied at the 1.5 leaf-stage of barnyardgrass at a rate of 3㎏ ai per ㏊ as single treatment, and the benzophenap showed 60% inhibition when it was applied at the coleoptile stage. While the lowest rate controlling the 1.5 leaf-stage barnyardgrasses by 98 to 100% of the butachlor and pretilachlor was 1.5㎏ and 200g per ㏊, respectively. All of the combinations of pyrazolate with butachlor, pyrazoxyfene with pretilachlor, and benzophenap with butachlor have shown synergistic interaction in controlling barnyardgrass on the Chisaka's isobole of 90% growth inhibition as well as on the Colby`s interaction efficacy data; synergism indices were 2.44, 1.62 and 1.52 in order. The dose combinations shown the maximal synergism were 1870g of pyrazolate with 140g of butachlor (1:0.075), 33008 of pyrazoxyfene with 338 of pretilachlor (1:0.01), and 3350g of benzophenap with 520g of butachlor (1:0.15) on the ai/㏊ basis.