• 생태와환경
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• 제55권1호
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• pp.76-83
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• 2022

기수갈고둥의 잠재적 서식지를 추정하고, 기수갈고둥의 존재 여부에 따라 저서성 거대무척추동물의 군집 구조를 확인하고자 했다. 기수갈고둥의 분포지는 환경부에서 수행 중인 "하구 수생태계 현황 조사 및 건강성 평가" 자료 중 2016년부터 2018년까지의 자료를 이용했다. 점유 모델은 기수갈고둥의 잠재적 서식지를 추정하기 위해 사용되었으며 저서성 거대무척추동물의 군집 구조를 확인하기 위해 4개의 다양성 지수가 사용되었다. 기수갈고둥은 남해안 일대와 동해안 일부 지역에서 발견되었으며, 이러한 양상은 기존 연구와 일치했다. 추가로 점유 모델 적용 결과 기수갈고둥의 잠재적 서식지가 서해안 일부 지역에 나타날 수 있음을 예측했다. 저서성 거대무척추동물의 군집 구조는 기수갈고둥이 채집되지 않은 지역에 비해 기수갈고둥이 채집된 지역에서 상대적으로 높았다. 따라서 제한된 지역에 서식하는 기수갈고둥을 보전할 때 점유 모델을 고려할 수 있으며, 기수갈고둥은 기수환경을 대표할 수 있는 생물로서 지표종으로 활용될 수 있는 것으로 판단된다.

• 환경영향평가
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• 제6권2호
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• pp.61-80
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• 1997

This study tried to propose the guidelines for the ecological preservation zoning in Korea. So some related laws and regulations were inspected, which were Natural Environment Preservation Act, Nature Park Act, Cultural Asset Conservation Act, Forest Act and Urban Planning Act. In these acts, I could find several concepts related to the ecosystem that are described as the protection area. But there aren't detailed and practical characteristics in those concepts. So for making the practical concept of ecosystem preservation, I considered Multiple Use Module, Wildlife habitat model, and Environmental evaluation model. Thorough this step, the process and methodology was established for evaluating and analysing. The potentiality of the GIS system was inspected. So the TM5 scene of the site was acquired and processed by ER-Mapper, Idrisi, Arc/Info and Arcview. And several digitized data were input by scanning and vecterizing. The Erdas format was mostly exchangeable to any program. The site is the Byonsan Peninsula National Park. The forest stand information and topographic data were digitized, types of which are forest year, DBH, density, slope, aspect etc. And also the watershed boundary, roads and paths, natural and cultural resources were mapped and analysed. Modelling of preservation suitability found the dispersed patterns for the best suitable zone through all the site. And the development potential areas were checked on downwatershed. This patterns are thought to result from the forest location for the wildlife habitat and the low altitude and no-steep slopes for developing. And Early warning system concept was introduced by overlapping these two patterns on the both potential area. As the conclusions, I proposed that the preservation zone be assigned according to the watershed unit as the main ecosystem zone. This main area should be linked by the eco-corridor through the point type eco-system. Finally, I thought the comprehensive information system should be established for making the rational and efficient decision making in natural area.

• 한국해양학회지:바다
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• 제21권4호
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• pp.125-133
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• 2016

본 연구는 통영연안의 오염 현황을 파악하기 위하여 표층퇴적물의 유기물 분포 특성, 미량금속의 공간적인 분포 특성과 오염도 및 생태계 위해성평가를 하였다. 총질소(Total nitrogen, TN), 총유기탄소(Total organic carbon, TOC)와 산 휘발성 황화물(Acid volatile sulfide, AVS)은 협수로에 위치한 정점 35-38에서 높은 농도를 나타내었다. Cd, Cr, Ni, Co, Hg, Zn의 공간적 분포양상은 유사하였으며, 이와 달리 Cu는 협수로에서 높은 농도를 나타내었다. 미량금속 원소의 오염도를 농축계수(Enrichment Factor, EF)로 평가한 결과, Cd이 모든 정점에서 오염되지 않은 상태(No enrichment), Pb, Cr, Ni, Co, Zn, Hg이 약간 오염상태(Minor enrichment), Cu가 약간~중간 오염상태(minor-moderate enrichment)의 오염도를 나타냈다. 미량금속에 의한 생태위해성 수준은 오비도와 미륵도 사이의 협수로 정점에서 국지적으로 생태계에 잠재적 위해성이 있는 것으로 나타났다.

• 생명과학회지
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• 제8권6호
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• pp.654-659
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• 1998

NR2B는 연접후 치밀질의 주요 tyrosine 인산화 단백질이다. 본 연구에서는 mass spectrometry 방법을 적용하여 NR2B의 tyrosine 인산화 위치를 동정하였다. NR2B를 N-octyl glucoside (NOG)에 용해되지 않는 PSD 분획으로부터 SDS-PAGE와 electroelution방법으로 분리하였다. 분리한 단백질을 NR2B와 phos-photyrosine에 특이한 항체로 조사한 결과 이들은 phosphotyrosine을 유지하고 있는 NR2B임이 확인되었다. 이 단백질을 trypsin 혹은 endolys-C 처리하고, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry 방법으로 조사한 결과 Tyr-1304이 인산화됨을 확인하였다.

• Radiation Oncology Journal
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• 제38권1호
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• pp.18-25
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• 2020

Purpose: This study was aim to evaluate the patterns of failure according to radiotherapy (RT) target volume for cervical lymph nodes in metastases of unknown primary origin in head and neck region (HNMUO). Materials and Methods: Sixty-two patients with HNMUO between 1998 and 2016 were retrospectively reviewed. We analyzed the clinical outcomes and primary site failure depending on the radiation target volume. The target volume was classified according to whether the potential head and neck mucosal sites were included and whether the neck node was treated involved side only or bilaterally. Results: Potential mucosal site RT (mucosal RT) was done to 23 patients and 39 patients did not receive mucosal RT. Mucosal RT showed no significant effect on overall survival (OS) and locoregional recurrence (LRR). The location of primary site failure encountered during follow-up period was found to be unpredictable and 75% of patients with recurrence received successful salvage therapies. No significant differences in OS and LRR were found between patients treated to unilateral (n = 35) and bilateral neck irradiation (n = 21). Treatment of both necks resulted in significantly higher mucositis. Conclusions: We found no advantages in OS and LRR of patients with HNMUO when mucosal sites and bilateral neck node were included in the radiation target volume.

• 한국물환경학회지
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• 제27권3호
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• pp.314-321
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• 2011

The potential effects of increased water temperature on fish assemblages and their habitats were studied in the streams of the Han River watershed when the water temperature was supposed to increase by $1^{\circ}C$, $2^{\circ}C$, and $3^{\circ}C$ in each sampling site. The percent changes in suitable habitats for each species and in species number within a site were determined, based on the estimated maximum thermal tolerances of 51 fish species whose habitats were classified into four reaches: upstream, up-/midstream, midstream, and mid-/downstream. The maximum thermal tolerance ranged between $25.0^{\circ}C$ and $31.0^{\circ}C$ and significantly increased as the habitat reaches move from upstream to mid-/downstream. With the increases in water temperature, the average suitable habitats for all 51 species were decreased by 31% ($+1^{\circ}C$), 46% ($+2^{\circ}C$), and 60% ($+3^{\circ}C$). The increased water temperature, however, did not induce significant differences in the changes in suitable habitats among four reaches within each level of temperature increase. The relative frequencies of the sampling sites classified into six levels according to the percent change in the number of species in each site were significantly different among three levels of water temperature increase, with relatively greater changes in the sites where 0~20% and 61~100% of species were affected.

• Bulletin of the Korean Chemical Society
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• 제24권6호
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• pp.817-823
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• 2003

We have performed ab initio calculation on the active site of HIV-1 protease. The FEP method was used to determine the binding free energy of four different of protonated states of HIV-1 protease with inhibitor. The structure of the active site and hole structure was taken from the X-ray crystallographic coordinates of the C₂ symmetric inhibitor A74704 protease bound. The active site was modeled with the fragment molecules of binding pocket, acetic acid/ acetate anion (Asp25, Asp125), formamide (amide bond of Thr26/Gly27, Thr126/ Gly127), and methanol as inhibitor fragment. All possibly protonated states of the active site were considered, which were diprotonated state (0, 0), monoprotonated (-1, 0),(0, -1) and diunprotonated state (-1, -1). Once the binding energy Debind, of each model was calculated, more probabilistic protonated states can be proposed from binding energy. From ab-initio results, the FEP simulations were performed for the three following mutations: Ⅰ) Asp25 … Asp125 → AspH25 … Asp125, ⅱ) Asp25 … Asp125 → Asp25 … AspH125, ⅲ) AspH25 … Asp125 → AspH25 … AspH125. The free energy difference between the four states gives the information of the more realistic protonated state of active site aspartic acid. These results provide a theoretical prediction of the protonation state of the catalytic aspartic residues for A74707 complex, and may be useful for the evaluation of potential therapeutic targets.

• 방사성폐기물학회지
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• 제5권4호
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• pp.351-357
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• 2007

일본 정부는 사용후핵연료의 중간저장시설의 입지로 아우모리현의 무쯔시를 선정했다. 2000년부터 시작된 유치활동이 5 년만에 결실을 맺은 것이다. 한국은 사용후핵연료를 원자력 발전소 내에 저장하고 있는데 2016년이면 저장한계에 이를 것으로 전망되고 있어 일본의 사례연구는 한국에 시사하는 바가 크다. 한국은 경주에 중, 저준위 방사성 폐기물 저장시설을 성공적으로 유치한 경험이 있어 사용후핵연료의 저장시설을 유치하는 학습경험을 축적한 바 있는데 두 나라의 큰 차이점은 한국은 경쟁적인 주민투표를 통하면서 막대한 지역지원금을 지원한다는 점이고 일본은 주민투표를 시행하지 않고 지방자치 정치의 리더쉽이 문제를 해결하는 방식을 택했다는 점이다. 엄청난 지역지원금이 지원되지 않은 점도 일본의 특징이다. 본 연구가 한국의 사용후핵 연료 저장시설을 유치하는 데 도움이 되었으면 하는 바람이다.

• 한국환경복원기술학회지
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• 제11권5호
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• pp.92-103
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• 2008

Development of new approaches to achieve naturally good ecological potential of forest road cut-slope by making the best use of advantages of gabion systems with vegetation base materials to prevent slope failure and erosion, in the area with highly erodible soil. As a result on the type analysis of gabion systems already installed in road cut-slopes, gabion systems were generally established to prevent slope failure. Existing gabion systems can be divided into monolithic and modular system and can be divided into ten subtypes according to the purpose of establishment and combination of other measures. As a result on the monitoring of erosion amount from forest road cut-slopes in the test applications, the order of erosion amount from largest to smallest is as follows : the curved road cut-slope site where normal gabion system was established ($7,911cm^3$); the control site ($7,632cm^3$); the straight road cut-slope site where normal gabion system was established ($7,301cm^3$); the curved road cut-slope site where the new gabion system was established ($5,684cm^3$); and the straight road cut-slope site where the new gabion system ($5,325cm^3$). Therefore, the result shows that the new gabion system is more effective than the normal gabion system to reduce erosion amount from forest ! road cut-slopes. During the study period, vegetation coverages of the straight and curved road cut-slope site where the new gabion system was established were about 45% and about 36%, so average vegetation coverage of the sites where the new gabion systems was established was higher than the sites where the normal gabion systems was established. Therefore, it was concluded that the new gabion system can be more effective for cut-slope revegetation.

• 한국가축번식학회지
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• 제25권4호
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• pp.381-388
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• 2001

본 연구는 돼지에서 성장호르몬과 결합되는 부위에 변이를 유도하여 결합력이 향상된 성장호르몬 결합단백질을 획득하기 위하여 수행되었다. 돼지의 지방으로부터 얻은 성장호르몬 수용체 RNA 내 성장호르몬 결합단백질 부분을 756 bp의 cDNA로 전향하고 클로닝한 후 site-directed mutagenesis 방법을 이용하여 26과 122번째 아미노산을 변이시켰다. 26번째 아미노산은 성장 호르몬과의 결합에 관련이 있다고 알려져 있는 돼지 성장호르몬 수용체 외막에 존재하는 다섯 군데의 N-linked glycosylation 부위와 가까이 위치한 부분이고, 122번째 아미노산은 소에서의 결합부위로 알려져 있다. 이렇게 변이를 유도한 성장호르몬 결합 단백질을 pET-32(c) 발현벡터에 삽입시키고 과발현시켰고 이를 정제하여 30 kDa의 변이를 유도한 성장호르몬 결합 단백질을 얻었다. 이러한 방법으로 성장호르몬 결합 단백질을 성장기에 있는 세포나 동물에 주입한다면 보다 향상된 성장을 볼 수 있을 것으로 사료된다.