• 한국발생생물학회지:발생과생식
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• 제14권3호
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• pp.199-205
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• 2010

A neurotoxin, 6-hydroxydopamine (6-OHDA) has been widely used to create animal model for Parkinson's disease (PD). The present study was undertaken to examine whether depletion of brain dopamine (DA) stores with 6-OHDA can make alteration in the activities of the testicular steroidogenesis in adult rats. Young adult male rats (3 months old) were received a single dose of 6-OHDA (200 ${\mu}g$ in 10 ${\mu}{\ell}$/animal) by intracerebroventricular (icv) injection, and sacrificed after two weeks. The mRNA levels of steroidogenesis-related enzymes were measured by qRT-PCRs. Serum testosterone levels were measured by radioimmunoassay. Single icv infusion of 6-OHDA significantly decreased the mRNA levels of CYP11A1 (control:6-OHDA group=$1:0.68{\pm}0.14$ AU, p<0.05), CYP17 (control:6-OHDA group=$1:0.72{\pm}0.13$ AU, p<0.05). There were no changes in the mRNA levels of $3{\beta}$-HSD (control:6-OHDA group=$1:0.84{\pm}0.08$ AU) and $17{\beta}$-HSD (control: 6-OHDA group=$1:0.63{\pm}0.20$ AU), though the levels tended to be decreased in the 6-OHDA treated group. Administration of 6-OHDA decreased significantly the mRNA level of StAR when compared to the level of saline-injected control animals (control:6-OHDA group=$1:0.72{\pm}0.08$ AU, p<0.05). Treatment with single dose of 6-OHDA remarkably lowered serum testosterone levels compared to the levels of control group (control:6-OHDA group=$0.72{\pm}0.24:0.13{\pm}0.03ng/m{\ell}$, p<0.05). Taken together with our previous study, the present study demonstrated that the activities of hypothalamus-pituitary-testis hormonal axis could be negatively affected by blockade of brain DA biosynthesis, and suggested the reduced reproductive potential might be resulted in the animals. More precise information on the testicular steroidogenic activities in PD patients and PD-like animals should be required prior to the generalization of the sex steroid hormone therapy to meet the highest standards for safety and efficacy.

• 한국발생생물학회지:발생과생식
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• 제24권3호
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• pp.231-239
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• 2020

Many benefits of silk protein fibroin (SPF) have been suggested in biomedical applications; and notably, significant SPF effects have been observed for metabolic syndromes that are directly linked to insulin resistance, such as type 2 diabetes mellitus (T2DM). Based on our previous findings, we believe that SPF from spiders exhibits outstanding glucose-lowering effects in diabetic BKS.Cg-m+/+Lepr^db mice. In order to evaluate the dietary effects of SPF in diabetic animals, we generated several lines of transgenic rice (TR) that expresses SPF, and the feeding of TR-SPF to diabetic animals decreased blood glucose levels, but did not change insulin levels. Western blot analyses of hepatic proteins showed that AMP-activated protein kinase (AMPK) expression and phosphorylation both decreased in TR-SPF-fed groups, compared with controls. This finding suggests that the glucose-lowering effects in this diabetic animal model might be AMPK-independent. In contrast, six-transmembrane protein of prostate 2 (STAMP2) was upregulated after TR-SPF exposure. Together with STAMP2, the Akt protein phosphorylation increased after TR-SPF exposure, which indicates that STAMP2 leads to Akt phosphorylation and thus increases insulin sensitivity in hepatocytes. Importantly, the hepatic steatosis that was seen in the liver of diabetic mice was remarkably alleviated in TR-SPF-fed mice. Hepatocytes that were immunopositive for STAMP2 were overwhelmingly observed in hepatic tissues from TR-SPF-fed mice compared to the control. Taken together, these results suggest that feeding diabetic mice with TR-SPF upregulates STAMP2 expression and increases Akt phosphorylation in hepatic tissues and thus potentially alleviates insulin resistance and hepatic steatosis.

• 자원환경지질
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• 제47권6호
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• pp.635-643
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• 2014

현재 우리나라의 지하수 개발가능량은 10년빈도 갈수시 함양량으로 결정되며 일정면적 이상의 수문학적 유역 또는 행정구역별로 넓게 구분되어 있어 소규모 유역의 지하수 영향평가에 적용하기가 어려운 면이 있다. 이에 본 연구에서는 셀기반의 지하수 개발가능량을 추정하는 새로운 기법을 경주지역에 대해 제시하였다. 이를 위해 지표수-지하수 통합 수문해석 모형인 SWAT-MODFLOW를 이용하여 셀기반 지하수 함양량을 산정하였다. 지하수 개발가능량을 결정하는 기준은 10년빈도 갈수시 강수량에 함양계수를 곱하여 추정하는 기존방식을 준용했다. 격자별 개발가능량을 산정한 후에는 목적에 따라 경주지역에서 143개로 세분화된 소유역별, 읍면리등 행정구역별로 개발가능량을 제시할 수 있었다. 지하수 이용량과 개발가능량을 연계하여 분석한 결과 경주시 전체 이용율은 평균 24%의 비교적 양호한 이용률을 보였으나 지역적으로는 7.1~108.8%까지 편차가 큰 이용현황을 보이는 것을 쉽게 확인할 수 있었다. 또한 임의의 시군구에서 지역별 추가 개발가능량도 평가할 수 있어 그 활용성이 기대된다.

• Nutrition Research and Practice
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• 제12권3호
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• pp.191-198
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• 2018

BACKGROUD/OBJECTIVES: Neuroinflammation plays critical role in neurodegenerative disorders, such as Alzheimer's disease (AD). We investigated the effect of three licorice varieties, Glycyrhiza uralensis, G. glabra, and Shinwongam (SW) on a mouse model of inflammation-induced memory and cognitive deficit. MATERIALS/METHODS: C57BL/6 mice were injected with lipopolysaccharide (LPS; 2.5 mg/kg, intraperitoneally) and orally administrated G. uralensis, G. glabra, and SW extract (150 mg/kg/day). SW, a new species of licorice in Korea, was combined with G. uralensis and G. glabra. Behavioral tests, including the T-maze, novel object recognition and Morris water maze, were carried out to assess learning and memory. In addition, the expressions of inflammation-related proteins in brain tissue were measured by western blotting. RESULTS: There was a significant decrease in spatial and objective recognition memory in LPS-induced cognitive impairment group, as measured by the T-maze and novel object recognition test; however, the administration of licorice ameliorated these deficits. In addition, licorice-treated groups exhibited improved learning and memory ability in the Morris water maze. Furthermore, LPS-injected mice had up-regulated pro-inflammatory proteins, such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2, interleukin-6, via activation of toll like receptor 4 (TLR4) and nuclear factor-kappa B ($NF{\kappa}B$) pathways in the brain. However, these were attenuated by following administration of the three licorice varieties. Interestingly, the SW-administered group showed greater inhibition of iNOS and TLR4 when compared with the other licorice varieties. Furthermore, there was a significant increase in the expression of brain-derived neurotrophic factor (BDNF) in the brain of LPS-induced cognitively impaired mice that were administered licorice, with the greatest effect following SW treatment. CONCLUSIONS: The three licorice varieties ameliorated the inflammation-induced cognitive dysfunction by down-regulating inflammatory proteins and up-regulating BDNF. These results suggest that licorice, in particular SW, could be potential therapeutic agents against cognitive impairment.

• 농촌계획
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• 제20권4호
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• pp.77-87
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• 2014

Ecotourism has the potential to boost the energy of a village as it pays keen attention to the ecosystem and the residents of the village. It is empowered by participation of and cooperation among stakeholders who are closely involved in ecotourism. However, many of them express difficulty in striking a right balance between development and conservation with regard to promoting ecotourism. Against this backdrop, this research paper investigates the structure of conflict that the stakeholders, especially those in Stork Village in Yesan County, South Chungcheong Province of Korea, experience during the process of establishment of government-led ecotourism. In addition, this study examines the problems of government-led ecotourism model and how they can be addressed. To analyze conflict structure, this paper used Q methods and found out that the budget-related stakeholders are largely divided into four groups as who; a) complain about how business profits are distributed; b) secure profits by expanding programs; c) consider human settlement, and; d) broaden people's participation. The biggest contributor to the conflicts is found that compensation was given discriminately to different jurisdictions. The second finding is that residents became less cooperative when the financial compensation did not live up to their expectation. For instance, they would demand the tourism facility physically expanded, repeatedly complain about the process of the work, and even accuse the government of degrading ecosystem. In other words, unless the compromise is reached with the residents regarding financial compensation, it could be difficult to encourage their participation and develop as a program-oriented tour. Lastly, the tour program needs to induce voluntary participation of the residents and deliver proper information on ecosystem and natural resources so as to last as sustainable ecotourism. The success of ecotourism will be subject to the cooperation of stakeholders in a region, conservation of our fragile ecosystem, and realization of sustainable growth through sharing economic benefits. This study looks into the cause of the conflicts of ecotourism sites and their structure. If this paper can bring about cooperation of stakeholders, the management and operation of ecotourism sites would be more sustainable.

• 폴리머
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• 제35권4호
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• pp.277-283
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• 2011

고체분산체는 난용성 약물의 용출률 개선을 위한 방법으로 주로 사용된다. 난용성 약물인 프란루카스트를 플라스돈 S-630과 함께 분무건조하여 고체분산체를 제조하였다. pH에 따른 프란루카스트 용해도 실험을 실시하여 높은 pH에서 약물의 용해도가 높게 나왔다. 입도 분석으로 고체분산체 내의 약물의 크기가 나노 크기로 작아진 것을 확인하였다. 표면전위를 측정하여 고체분산체가 음전하를 가지고 있는 것을 확인하였다. 주사전자현미경으로 고체분산체의 표면이 구형임을 확인하였고, 시차주사열량계와 X-선 회절 분석법을 통해 고체분산체가 무정형임을 확인하였다. 고체분산체의 용출특성을 알아보기 위해 인공장액(pH 6.8)에서 용출거동을 확인하였고, 대조실험을 위해 시판제인 오논$^{(R)}$캡슐을 사용하였다. 이 결과로 분무건조를 통한 고체분산제의 제조를 통해 난용성 약물의 용출특성을 확인하였고, 경구용 약제학적 형태를 가질 수 있는 것을 확인하였다.

• Archives of Plastic Surgery
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• 제36권5호
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• pp.531-537
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• 2009

Purpose: Alveolar bone grafting has become an essential process in the treatmemt of alveolar cleft patient for stabilization of the maxillary arch, elimination of oronasal fistula, the reconstruction of the soft tissue nasal base support, and creation of bony support for tooth eruption for implant. The use of Autologous iliac cancellous bone is preferable because of the adequate quantity and high osteoinductive potential. However, even with iliac bone, insufficient osteoregeneration and absorption occur due to several factors such as the patient's age, cleft width, functional stress, and others. In order to increase osteoregeneration where the iliac bone is placed, the present study is associated with bone marrow aspirate (BMA). The experimental study evaluated the efficacy of osteoregeneration in normal cleft rabbits when alveolar bone grafting was performed with autologous iliac corticocancellous bone. Methods: Twenty - four New Zealand White rabbits were divided randomly into 2 groups (BMA, control). All animals underwent harvesting of corticocancellous bone graft from the right posterior iliac crest via standard surgical technique. $1m{\ell}$ of BMA were obtained by scraping the needle and aspirate with $10m{\ell}$ syringe from the contralateral iliac bone wall. The muco - periosteal flap on the palate was elevated. A mixture of Equal bone's volumes with BMA and saline as its control was inserted into the cleft. Animals were sacrificed at 2, 4, and 8 weeks and maxilla was harvested for dental peri - apical X-ray, bone matrix density (BMD),and histologic analysis. Result: BMD of regenerated bone to the cleft in the rabbits was higher than that of the control rabbits. X-ray, histologic analysis showed that increased osteoregeneration and low absorption rate were observed in the BMA group. Conclusion: Our experimental study showed BMA enhanced the osteoregeneration and survival rate of alveolar bone grafting. BMA is easy to extract & cost - time effective. So it can be an effective enhancers for bone grafting mixtures.

• 한국수자원학회논문집
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• 제43권8호
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• pp.681-694
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• 2010

본 연구에서는 경제성분석에 의한 강변저류지의 최적위치 선정기법을 제안하였다. 강변저류지 설치에 소요되는 비용을 크게 건설비용과 토지매입비용으로 나누어 산정하였으며, 강변저류지 설치로 인해 발생하는 이익을 홍수피해저감액과 습지설치로 인한 연간이득으로 구분하여 산정하였다. 홍수량-홍수피해액 관계를 간접적으로 도출하는 방안을 제시하였으며, 강변저류지의 습지활용 시에 발생하는 이익은 인공습지의 가치평가기법을 도입하여 평가하였다. 경제성평가 기법으로는 편익-비용분석을 적용하였으며, 안성천 유역의 13개 후보강변저류지에 대한 경제성평가를 수행하였다. 또한 강변저류지의 최적 위치와 규모를 결정하기 위해 홍수저감효과를 이용한 기존의 모형을 보완하여, 경제성평가기법을 이용한 의사결정기법을 제안하였다. 제안된 의사결정기법을 안성천유역의 13개 후보강변저류지를 대상으로 적용하였으며, 기존에 홍수저감효과만을 이용한 모형의 결과와 비교분석하였다. 그 결과 홍수저감량만을 고려한 기존의 결과와 비교할 때 보다 높은 경제성을 제공할 수 있는 최적조합선정이 가능하였고, 경제성을 고려하는 것이 보다 실질적인 의사결정의 기준으로 사용될 수 있다는 것으로 나타났다.

• 동의생리병리학회지
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• 제25권2호
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• pp.300-305
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• 2011

The present study was investigated the neuroprotective effects of acupuncture at ST36 on learning and memory deficits after transient cerebral ischemia in a gerbil model. The animals were randomly divided into three groups (n=7 in each group): the sham operation group (SHAM), ischemia-induced and ST36 acupuncture group (ISC + ST36), and the ischemia-induced and Tail-acupuncture group (ISC + TAIL). For the acupuncture stimulation, stainless steel needles, 0.3 mm in diameter, were inserted bilaterally into the ST36 locus or the tail and stimulated for 1 min/day for 14 days. Using the Morris water maze test, the animals were tested on spatial learning and memory. In addition, the effects of acupuncture on memory storage and the choline acetyltransferase (ChAT) activity, in the hippocampal CA1 area, were investigated by ChAT immunohistochemistry. Transient cerebral ischemia produced impaired performance on the MWM test (DAY 5: p<0.01 and retention test: p<0.05) and severely decreased ChAT immunoreactivity in the CA1 hippocampal area compared to the SHAM group (p<0.05). However, improved learning and memory were observed (DAY 5: p<0.05 and retention test: p<0.01) as well as a significantly reduced loss of ChAT immunoactivity in the hippocampal CA1 region (p<0.001) after acupuncture stimulation at ST36 were observed. These results show that acupuncture at ST36 ameliorated the learning and memory deficits at least in part through the cholinergic system. The findings of this study provide potential data that acupuncture is useful for the treatment of some of the behavioral impairs of transient cerebral ischemia.

• 한국측량학회지
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• 제32권4_1호
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• pp.319-326
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• 2014

고해상도 위성영상을 성공적으로 활용하기 위해서는 지상기준점 등을 활용한 좌표등록 및 보정 과정이 필수적이다. 작업자의 수작업을 통한 기준점 획득의 경우 작업 시간이 오래 걸리므로, 자동화된 좌표 등록 방법에 대한 요구가 증대하고 있다. 보정하고자 하는 위성 영상을 정확한 좌표를 가진 참조 데이터에 영상 매칭을 수행하는 기법이 많이 소개 되었는데, 참조 데이터 중 라이다 데이터의 경우 공간 해상도 및 정확도가 높고 무엇보다 3차원 데이터이기 때문에 기복 변위 등을 내포하고 있지 않는 등의 장점을 보인다. 최근 라이다 데이터와 고해상도 위성영상간의 매칭을 위한 기법이 연구, 발표되었으나, 라이다 데이터의 특성상 대용량이기 때문에 처리에 많은 시간이 소요되는 등의 단점이 있었다. 따라서 본 논문에서는 일부의 공간만을 라이다 칩으로 추출 및 저장하여 위성영상의 좌표 등록에 활용하는 연구를 수행하였다. 이를 위해, 전체 라이다 포인트 데이터를 반사강도 정사영상 및 수치표고모델의 두 가지 형태로 변환하고 에지 추출을 통해 의미 있는 양의 에지 정보만을 포함하는 지역을 영상형태의 라이다 칩으로 추출, 저장하였으며, 용량이 현저히 줄어든 것을 확인할 수 있었다. 마지막으로 라이다 칩을 아리랑2호 및 아리랑3호 영상의 자동 좌표등록에 활용 해본 결과 평균 한 픽셀가량의 정확도 또한 확보할 수 있었다.