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Effect of Bone Marrow Aspirate with Autogenous Bone graft for Alveolar Cleft in a new Rabbit Model  

Bae, Sung Gun (Department of Plastic and Reconstructive Surgery, Kyungpook National University)
Chung, Ho Yun (Department of Plastic and Reconstructive Surgery, Kyungpook National University)
Lee, Sang Yun (Department of Plastic and Reconstructive Surgery, Kyungpook National University)
Cho, Byoung Chae (Department of Plastic and Reconstructive Surgery, Kyungpook National University)
Yang, Jung Dug (Department of Plastic and Reconstructive Surgery, Kyungpook National University)
Park, Mee Young (Department of Neurology College of Medicine, Yeung Nam University)
Publication Information
Archives of Plastic Surgery / v.36, no.5, 2009 , pp. 531-537 More about this Journal
Abstract
Purpose: Alveolar bone grafting has become an essential process in the treatmemt of alveolar cleft patient for stabilization of the maxillary arch, elimination of oronasal fistula, the reconstruction of the soft tissue nasal base support, and creation of bony support for tooth eruption for implant. The use of Autologous iliac cancellous bone is preferable because of the adequate quantity and high osteoinductive potential. However, even with iliac bone, insufficient osteoregeneration and absorption occur due to several factors such as the patient's age, cleft width, functional stress, and others. In order to increase osteoregeneration where the iliac bone is placed, the present study is associated with bone marrow aspirate (BMA). The experimental study evaluated the efficacy of osteoregeneration in normal cleft rabbits when alveolar bone grafting was performed with autologous iliac corticocancellous bone. Methods: Twenty - four New Zealand White rabbits were divided randomly into 2 groups (BMA, control). All animals underwent harvesting of corticocancellous bone graft from the right posterior iliac crest via standard surgical technique. $1m{\ell}$ of BMA were obtained by scraping the needle and aspirate with $10m{\ell}$ syringe from the contralateral iliac bone wall. The muco - periosteal flap on the palate was elevated. A mixture of Equal bone's volumes with BMA and saline as its control was inserted into the cleft. Animals were sacrificed at 2, 4, and 8 weeks and maxilla was harvested for dental peri - apical X-ray, bone matrix density (BMD),and histologic analysis. Result: BMD of regenerated bone to the cleft in the rabbits was higher than that of the control rabbits. X-ray, histologic analysis showed that increased osteoregeneration and low absorption rate were observed in the BMA group. Conclusion: Our experimental study showed BMA enhanced the osteoregeneration and survival rate of alveolar bone grafting. BMA is easy to extract & cost - time effective. So it can be an effective enhancers for bone grafting mixtures.
Keywords
Alveolar cleft; Bone marrow aspirate; Bone transplantation;
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1 Schmitz JP, Hollinger JO: The critical size defect as an experimental model for craniomandibulofacial nonunions. Clin Orthop Relat Res 205: 299, 1986   PUBMED   ScienceOn
2 Lindholm TS, Nilsson OS, Lindholm TC: Extraskeletal and intraskeletal new bone formation induced by demineralized bone marix combined with bone marrow cells. Clin Orthop Relat Res 171: 251, 1982   PUBMED   ScienceOn
3 Freng A: Single layered periosteoplasty in experimental mid-palatal clefts. a histological study in the cat. Scand J Plast Reconstr Surg 13: 401, 1979   DOI   PUBMED
4 Soltan M, Smiler D, Choi JH: Bone marrow: orchestrated cells, cytokines, and growth factors for bone regeneration. Implant Dent 18: 132, 2009   DOI   ScienceOn
5 Rullo R, Festa VM, Guida L, Laino G: Bone grafting with platelet-rich plasma in alveolar cleft. Case report. Minerva Stomatol 56: 63, 2007   PUBMED   ScienceOn
6 Smiler D, Soltan M: The bone-grafting decision tree: a systematic methodology for achieving new bone. Implant Dent 15: 122, 2006   DOI   ScienceOn
7 Boyne PJ, Sands NR: Secondary bone grafting of residual alveolar and palatal cleft. J Oral Surg 30: 87, 1972
8 el-Bokle D, Smith SJ, Germane N, Sharawy M: New technique for creating permanent experimental alveolar clefts in a rabbit model. Cleft Palate Craniofac J 30: 542, 1993   DOI   ScienceOn
9 Smiler D, Soltan M: Bone marrow aspiration: technique, graft, and reports. Implant Dent 15: 229, 2006   DOI   ScienceOn
10 Schultz RC: Surgically produced cleft palates in rabbits. A study of resulting middle ear infections. Plast Reconstr Surg 33: 120, 1964   DOI   PUBMED   ScienceOn
11 Aurouze C, Moller KT, Bevis RR, Rehm K, Rudney J: The presurgical status of the alveolar cleft and success of secondary bone grafting. Cleft Palate Craniofac J 37: 179, 2000   DOI   ScienceOn
12 Cho BC: Cleft treatment protocol in korea. J Korean Cleft Palate-Craniofac Assoc 6: 27, 2005
13 Soltan M, Smiler DG, Gailanil F: A new 'plantinum' standard for bone grafting: autogenous stem cells. Implant Dent 14: 322, 2005   DOI   PUBMED   ScienceOn
14 Jonsson G, Stenstrom S: Maxillary growth after palatal surgery. an experimental study on dogs. Scand J Plast Reconstr Surg 12: 131, 1978   DOI   PUBMED
15 Jakse N, Tangl S, Gilli R, Berghold A, Lorenzoni M, Eskici A, Haas R, Pertl C: Influence of PRP on autogenous sinus graft. an experimental study on sheep. Clin Oral Implants Res 14: 578, 2003   DOI   ScienceOn
16 Filho Cerruti H, Kerkis I, Kerkis A, Tatsui NH, da Costa Neves A, Bueno DF, da Silva MC: Allogenous bone grafts improved by bone marrow stem cells and platelet growth factors: clinical case reports. Artif Organs 31: 268, 2007   DOI   ScienceOn
17 Harvold E: Cleft palate, an experiment. Acta Odontol Scand 9: 84, 1950   DOI   PUBMED   ScienceOn
18 Caplan AI: Mesenchymal stem cells. J Orthop Res 9: