• 한국응용곤충학회지
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• 제13권1호
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• pp.41-45
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• 1974

우리나라 씨감자에 발생하고 있는 잠복성바이러스의 종류와 이들의 감염상태를 조사하기 위하여 감자 원원종포, 원종포 그리고 원예시험장 등에서 수집한 외관상 무병 건전하게 보이는 감자와 외관상 바이러스병징이 뚜렷한 감자를 대상으로 혈청학적방법에 의해 바이러스 검정을 실시했다. 검정대상 바이러스는 Potato Virus X(PVX), Potato Virus S(PVS), Potato Virus M(PVM), 그리고 Potato Virus Y (PVY)의 4종이며, 바이러스의 검정은 van Slogteren의 미량침강법에 따라 실시했다. 결과는 다음과 같다. 1. 씨감자 생산포장에서 수집한 감자잎에서 PVX, PVS, PVM, 그리고 PVY가 검출되었는데 우리나라 감자에서 PVM이 검출되었다는 실험적 보고는 이것이 처음이다. 2. 외관상 건전하게 보이는 씨감자의 거의 $100\%$ PVX와 PVS에 약 $10\%$가 PVX+PVS+PVM에, 약 $4.5\%$가 PVX+PVS+PVY에, 그리고 $1\%$ 정도가 PVX+PVS+PVM+PVY에 각각 혼합감염되어 있었다. 3. PVX와 PVS만이 검출된 식물은 대부분 병징이 음폐되어 있었으나 PVX, PVS와 함께 PVY가 검출된 식물은 대부분 뚜렷한 병징을 나타내고 있었다. 4. 혈청학적 미량탐강법은 육안적인 관찰만으로는 검정이 어려운 PVX, PVS, PVM 및 mild strain의 PVY를 씨감자 생산지에서 대규모의 식물은 대상으로 신속정확히 검정하는데 애우 편리한 방법으로 생각된다.

• 식물병연구
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• 제19권2호
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• pp.90-94
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• 2013

하령 감자는 전분함량이 높고, 역병에 저항성이며 맛이 좋은 품종으로 2005년도에 신품종으로 등록되었다. 2010년 저장 중인 하령 씨감자에서 심한 표피의 괴저와 표면이 융기되고 원형의 괴저 병반이 생기는 괴경 괴저 증상이 발생하였다. 괴경 괴저 증상의 감자를 PVY 진단용 프라이머를 이용한 RT-PCR 분석 결과 모두 PVY가 검출되었다. 괴저증상 감자에서 검출된 $PVY^{Hkr}$ 외피단백질의 유전자 염기서열을 분석하였고 $PVY^{Kor}$, $PVY^N$, $PVY^{NTN}$, $PVY^O$, $PVY^C$ 계통과 상동성을 비교한 결과 $PVY^{Hkr}$은 2005년에 보고된 $PVY^{Kor}$와 2개의 염기를 제외하고 정확히 일치하였다. PVY 감염이 저장 중인 하령 품종에서 같은 병징을 일으키는지 확인하기 위해 5품종의 감자(하령, 수미, 대서, 대지, 추백)와 2종의 바이러스(PVY, PLRV)를 이용하여 생물검정을 실시하였다. 그 결과, 괴경 괴저 증상은 PVY에 감염된 저장 중인 하령 품종에서만 나타났다.

• 한국연초학회지
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• 제23권2호
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• pp.115-122
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• 2001

The effect of mineral oil treatment in Burley 21 tobacco field on the control of potato virus Y(PVY-VN) mostly transmitted by green peach apid(Myzus persicae Sulzer) in nature was studied and the virus infection in some plants including potato, pepper, bramble, radish, etc near the tobacco fields as a virus infection source was tested by capillary tube precipitatioin test with PVY-antibody and bioassay in Xanthi-nc tobacco. The main source of PVY-VN infection in tobacco field in korea was potato(ca. 40% of test plants infected). Pepper and bramble were also infected by PVY-VN. The control level of PVY-VN infection by treatment of 0.75% liquid mineral oil with 3 % nonionic emulsifier to the plants was 84.8 % in case of the artificial transfection with a infected apterous aphid in laboratory. However, the reduction of PVY-VN disease severity in tobacco fields treated with mineral oil at late June was only 35.5%. These results suggest that mineral oil treatment is not so effective for the protection of aphid-born virus(PVY - VN) infection in tobacco fields.

• 한국연초학회지
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• 제5권1호
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• pp.19-23
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• 1983

Potato virus Y (PVY) distribution, areas where the virus occurred, and incidence, Percentage of plants infected, on burley tobacco in Korea was surveyed in 1982. Most of the fields Investigated were infected with PVY. The virus incidence was 12.5%. District)union and incidence generally were sporadic, but Onyang, Hongseung and Iksan area virus incidence was higher than that of other areas. For strain identification, approximately 95% was nonnecrotic (PVY-VB) and 5olo necrotic strain (PVY-VN) .

• 식물병연구
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• 제9권1호
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• pp.1-9
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• 2003

The occurrence of potato(Sotanum tuberosum) viral diseases caused by Potato virus X(PVX), Potato virus Y (PVY), Potato leafroll virus(PLRV), Potato vims S(PVS), Potato virus M(PVM), Potato virus A(PVA), Potato virus T(PVT), Alfalfa mosic virus(AIMV), Tobacco mosic virus(TMV), Potato mop top virus(PMTV) Tobacco rattle virus(TRV) and Potato spindle tuber viroid(PSTVd), potato witches' broom phytoplasma, have been identified so far in Korea. Major viral diseases such as PVX, PVY and PLRV had been studied more deeply, however, the others are just identified and only partially characterized since the first study on the relation between PVX nucleic acid and virus protein by Kim in 1961. The most studies on potato viral diseases are mainly focused on the problems of seed potato production. The National Alpine Agricultural Experiment Station(NAAES), since it began its activities in 1961, has given special attention to this problem by doing studies to identify, characterize and control potato virus diseases. This effort resulted in the development of new potato virus detection methods as a basis for elaborating new method of control, such as the production of seed potato free of virus and the selection of new virus-resistant transgenic potatoes. The further studies of potato viral diseases required would be fallowings: the continuous monitoring for the occurrence of identified or not identified potato viruses in Korea, the isolation of resistant viral genes, the development of control method for the non-persistently transmitted viruses like PVY, special vectors such as nematode and fungus transmitted viruses, TRV and PMTV and the development of control methods against potato viral diseases by viral cross protection, therapy, transgenic plant, and the use of the agents or molecules, such as virus inhibitors and antiviral proteins, etc., blocking viral replication.

• 한국작물학회지
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• 제59권4호
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• pp.498-504
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• 2014

Potato virus Y (PVY) and potato leafroll virus (PLRV) are among the most damaging potato viruses and prevalent in most potato growing areas. In this study, cryopreservation was used to eradicate PVY and PLRV using two cryogenic methods. Potato shoot tips proliferated in vitro were cryopreserved through droplet-vitrification and encapsulation-vitrification using plant vitrification solution 2 (PVS2; 30% glycerol + 15% dimethyl sulfoxide + 15.0% ethylene glycol + 13.7% sucrose) and modified PVS2. Both cryogenic procedures produced similar rates of survival and regrowth, which were lower than those from shoot tip culture alone. The health status of plantlets regenerated from shoot tip culture alone and cryopreservation was checked by reverse transcription-polymerase chain reaction. The frequency of virus-free plants regenerated directly from highly proliferating shoot tips reached 42.3% and 48.6% for PVY and PLRV, respectively. In comparison, the frequency of PVY and PLRV eradication after cryopreservation was 91.3~99.7% following shoot-tip culture. The highest cryopreserved shoot tip regeneration rate was observed when shoot tips were 1.0~1.5 mm in length, but virus eradication rates were very similar (96.4~99.7%), regardless of shoot tip size. This efficient cryotherapy protocol developed to eliminate viruses can also be used to prepare potato material for safe long-term preservation and the production of virus-free plants.

• The Plant Pathology Journal
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• 제33권4호
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• pp.382-392
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• 2017

This study reports the findings of a distinct Potato virus Y (PVY) isolate found in Northeast China. One hundred and ten samples (leaves and tubers) were collected from potato plants showing mosaic symptoms around the city of Harbin in Heilongjiang province of China. The collected tubers were planted and let to grow in a greenhouse. New potato plants generated from these tubers showed similar symptoms, except for one plant. Subsequent serological analyses revealed PVY as the causing agent of the disease. A novel PVY isolate (referred to as HLJ-C-44 in this study) was isolated from this sample showing unique mild mosaic and crisped leaf margin symptoms. The complete genome of this isolate was analyzed and determined. The results showed that HLJ-C-44 is a typical PVY isolate. Phylogenetic analysis indicated that this isolate belongs to the N-Wi strain group of PVY recombinants ($PVY^{N-Wi}$) and also shared the highest overall sequence identity (nucleotide and amino acid) with other members of this strain group. However, recombination analysis of isolate HLJ-C-44 revealed a recombination pattern that differed from that of other $PVY^{N-Wi}$ isolates. Moreover, biological assays in four different potato cultivars and in Nicotiana tabacum also revealed a different phenotypic response than that of a typical $PVY^{N-Wi}$ isolate. This data, combined, suggest that HLJ-C-44 is a novel PVY recombinant with distinct biological properties.

• 한국식물병리학회지
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• 제13권4호
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• pp.219-224
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• 1997

여러 계통의 PVY 외피단백질 유전자간에 상동성이 높은 부위에 위치한 primer 쌍을 이용하여 RT-PCR 방법으로 PVY 검정법을 개발하였는데 여러 line의 대서품종으로부터 764 bp의 PCR 산물이 합성되었고 이 절편을 sequencing한 결가 PVY의 유전자임을 확인하였다. 싹과 괴경 조직에서 RT-PCR에 의한 PVY의 검정의 민감도는 ELISA 방법보다 약 1,000배정도 높았다.

• 식물병연구
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• 제12권1호
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• pp.28-31
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• 2006

최근 감자 바이러스의 발생 양상은 과거에는 PLRV의 이병율이 높았으나 현재에는 PVY의 발생이 많아지고 있는 추세인데 이는 '대서'와 '추백' 같은 감수성 품종의 재배 면적의 증가와 바이러스 이병율이 높은 재배 환경에서의 채종 때문일 것이다. 특히 2기작 감자 포장에서의 당대 감염율을 보더라도 '대서'와 '추백'의 이병율이 높아 감수성 품종으로 '수미'와 '대지'는 이병율이 비교적 낮아 저항성으로 나타났다. 또한 2기작 감자 재배지대에서 PVY에 감수성인 '추백' 품종의 안전한 씨감자 생산을 위해서 주위에 일반감자 재배가 없는 격리가 양호한 환경에서의 채종과 그렇지 못한 환경에서의 채종을 비교하여 재감염 정도를 조사하였는데 조직 배양후 2세대 걸친 노지 재배에서 바이러스 이병율이 약 3배의 차이를 보여 씨감자 생산에서 채종 환경의 중요성을 입증한 결과로 판명되었다.

• 한국식물병리학회지
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• 제11권1호
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• pp.73-79
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• 1995

Complementary DNAs (cDNAs) to the coat protein gene of an ordinary Korean strain of potato virus Y (PVY-OK) isolated from potato (cv. Superior) were synthesized and cloned into a plasmid pUC119 and sequenced. The RNA of the virus propagated in tobacco (Nicotinaa sylvestris) was extracted by the method of phenol extraction. The first strand of cDNAs to the coat protein penomic RNA of the virus was made by Moloney murine leukemia virus reverse transcriptase. The cDNA were synthesized and amplified by the method of polymerase chain reaction (PCR) using a pair of oligonucleotide primers. PVYCP3P and PVYCP3M. The size of cDNAs inserted in pUC119 plasmid was estimated as about 840 bp upon agarose gel electrophoresis. Double stranded cDNAs were transformed into the competent cell of E. coli JM109. Sequence analysis of cDNAs was conducted by the dideoxynucleotide chain termination method. Homology of cDNAs of the PVY-OK coat protein genomic RNA with those of PVY-O (Japan), PVY-T (Japan), PVY-TH (Japan), PVYN (The Netherlands),and PVYY (France) was represented as 97.3%, 88.9%, 89.3%, 89.6% and 98.5%, respectively. Homology at the amino acid level turned out to the be 97.4%, 92.5%, 92.9%, 92.9% and 98.5%, respectively.