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http://dx.doi.org/10.5423/RPD.2013.19.2.090

Superficial Tuber Necrosis in Potato Cultivar 'Haryeong' Caused by Potato virus Y  

Lee, Young-Gyu (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration)
Kim, Jeom-Soon (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration)
Kim, Ju-Il (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration)
Park, Young-Eun (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration)
Publication Information
Research in Plant Disease / v.19, no.2, 2013 , pp. 90-94 More about this Journal
Abstract
Potato cv. 'Haryeong' was bred with high solids, resistance to late blight and good culinary quality. It has been registered as new potato variety in 2005. Tuber necrosis symptoms such as severe superficial necrosis, raised surface lesions and ringed necrotic areas were found in tubers of 'Haryeong' during storage of seed potatoes in 2010. Potato virus Y (PVY) was detected from these symptomatic tubers by the analysis of RT-PCR using a primer set specific to coat protein gene of PVY. The nucleotide sequence of RT-PCR product ($PVY^{Hkr}$) was determined and compared to those of other strains, such as $PVY^{Kor}$, $PVY^N$, $PVY^{NTN}$, $PVY^O$, and $PVY^C$ registered in GeneBank. The result showed that $PVY^{Hkr}$ was exactly the same as $PVY^{Kor}$, Korean isolate reported in 2005, except two nucleotides. To verify the PVY was responsible for the tuber necrosis symptoms shown in the tubers of 'Haryeong', a bioassay was done using two viruses (PVY and Potato leafroll virus) and five potato cultivars ('Haryeong', 'Superior', 'Atlantic', 'Dejima', and 'Chubaek'). As expected, the same necrosis symptom appeared in tubers of 'Haryeong' infected with PVY only during the storage period.
Keywords
Haryeong; Potato; Potato virus Y (PVY); Tuber necrosis symptom; Virus;
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1 Struik, P. C. and Wiersema, S. G. 1999. Quality control and seed certification. pp. 269-293. In: Seed Potato Technology. Wageningen Pers. Holland.
2 Boonham, N., Walsh, K., Preston, S., North, J., Smith, P. and Barker, I. 2002. The detection of tuber necrotic isolates of Potato virus Y, and the accurate discrimination of PVY (O), PVY (N) and PVY (C) strains using RT-PCR. J. Virol. Methods 102: 103−112.   DOI   ScienceOn
3 De Bokx, J. A. and Huttinga, H. 1981. Potato virus Y. CMI/AAB Descriptions of Plant Viruses No. 242.
4 Hahm, Y. I. 2003. Review on the occurrence and studies of potato viral disease in Korea. Res. Plant Dis. 9: 1−9.   DOI   ScienceOn
5 Jeffries, C. 1998. FAO/IPGRI Technical Guidelines for the Safe Movement of Germplasm. No. 19. Potato. Food and Agriculture Organization of the United Nations, Rome/International Plant Genetic Resources Institute, Rome.
6 Le Romancer, M. and Nedelee, M. 1994. Biological characterization of various geographical isolates of Potato virus Y inducing superficial necrosis on potato tubers. Plant Pathol. 43: 138-144.   DOI   ScienceOn
7 Lee, Y. G., Choi, J. A, Lee, J. S., Yoon, Y. N., Cheon, J. U. and Lee, K. W. 2005. Abnomal tubers symptoms in the solanum tuberosum cv. Chubaek by Potato virus Y - N strain (PVY-N). Korean J. Plant Pathol. 21: 424-425.
8 Park, Y. E., Cho, J. H., Cho, H. M., Ryu, K. Y., Yi, J. Y., Suh, H. W., Ahn, Y. K., Lim, H. K., Kim, H. S. and Choi, S. J. 2006. A new potato cultivar, "Haryeong" with resistance to late blight and good culinary taste of steamed potato. Korean J. Breed. 38: 213-214.   과학기술학회마을
9 Robaglia, C., Durand-Tardif, M., Tronchet, M., Boudazin, G., Astier-Manifacier, S. and Casse-Delbart, F. 1989. Nucleotide sequence of Potato virus Y (N strain) genomic RNA. J. Gen. Virol. 70: 935-947.   DOI   ScienceOn
10 Salazar, L. F. 1996. Ecology, epidermiology, and control of virus diseases. pp. 167-205. In: Potato Viruses and Their Control. CIP Lima. Peru.
11 Schubert, J., Fomitcheva, V. and Sztangret-Wisniewska, J. 2007. Differentiation of Potato virus Y strains using improved sets of diagnostic PCR-primers. J. Virol. Methods 140: 66-74.   DOI   ScienceOn