• Journal of Dental Anesthesia and Pain Medicine
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• v.15 no.4
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• pp.229-233
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• 2015

Background: Although water chambers are often used as surrogate blood-warming devices to facilitate rapid warming of red blood cells (RBCs), these cells may be damaged if overheated. Moreover, filtered and irradiated RBCs may be damaged during the warming process, resulting in excessive hemolysis and extracellular potassium release. Methods: Using hand-held syringes, each unit of irradiated and leukocyte-filtered RBCs was rapidly passed through a water chamber set to different temperatures (baseline before blood warming, $50^{\circ}C$, $60^{\circ}C$, and $70^{\circ}C$). The resulting plasma potassium and free hemoglobin levels were then measured. Results: Warming RBCs to $60^{\circ}C$ and $70^{\circ}C$ induced significant increases in free hemoglobin (median [interquartile ranges] = 60.5 mg/dl [34.9-101.4] and 570.2 mg/dl [115.6-2289.7], respectively). Potassium levels after warming to $70^{\circ}C$ ($31.4{\pm}7.6mEq/L$) were significantly higher compared with baseline ($29.7{\pm}7.1mEq/L$; P = 0.029). Potassium levels were significantly correlated with storage duration after warming to $50^{\circ}C$ and $60^{\circ}C$ (r = 0.450 and P = 0.001; r = 0.351 and P = 0.015, respectively). Conclusions: Rapid warming of irradiated leukoreduced RBCs to $50^{\circ}C$ may not further increase the extracellular release of hemoglobin or potassium. However, irradiated leukoreduced RBCs that have been in storage for long periods of time and contain higher levels of potassium should be infused with caution.

• Journal of Biosystems Engineering
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• v.31 no.6 s.119
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• pp.463-473
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• 2006

Automated sensing of soil macronutrients would allow more efficient mapping of soil nutrient spatial variability for variable-rate nutrient management. The capabilities of ion-selective electrodes for sensing macronutrients in soil extracts can be affected by the presence of other ions in the soil itself as well as by high concentrations of ions in soil extractants. Adoption of automated, on-the-go sensing of soil nutrients would be enhanced if a single extracting solution could be used for the concurrent extraction of multiple soil macronutrients. This paper reports on the ability of the Kelowna extractant to extract macronutrients (N, P, and K) from US Corn Belt soils and whether previously developed PVC-based nitrate and potassium ion-selective electrodes could determine the nitrate and potassium concentrations in soil extracts obtained using the Kelowna extractant. The extraction efficiencies of nitrate-N and phosphorus obtained with the Kelowna solution for seven US Corn Belt soils were comparable to those obtained with IM KCI and Mehlich III solutions when measured with automated ion and ICP analyzers, respectively. However, the potassium levels extracted with the Kelowna extractant were, on average, 42% less than those obtained with the Mehlich III solution. Nevertheless, it was expected that Kelowna could extract proportional amounts of potassium ion due to a strong linear relationship ($r^2$ = 0.96). Use of the PVC-based nitrate and potassium ion-selective electrodes proved to be feasible in measuring nitrate-N and potassium ions in Kelowna - soil extracts with almost 1 : 1 relationships and high coefficients of determination ($r^2$ > 0.9) between the levels of nitrate-N and potassium obtained with the ion-selective electrodes and standard analytical instruments.

• Korean Journal of Community Nutrition
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• v.2 no.2
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• pp.133-140
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• 1997

The serum concentrations of the major minerals(calcium, phosphorus, magnesium, sodium, potassium) and trace elements(iron, Zinc, copper manganese)were datermined in 23 breast fed infants living in Eumsung, Choong-buk region. The results obtained are summarized as follows: 1)The mean levels of calcium, phosphorus, magnesium, sodium and potassium of the serum of total subjects were 8.15$\pm$0.33mg/dI, 11.06$\pm$0.16mg/dI, 2.00$\pm$0.14mg/dI, 3.4476$\pm$17.99mg/dI and 9.06$\pm$2.04mg/dI respectively. 2)The serum concentrations of iron, zinc, copper and manganese in total subjects averaged 95.83$\pm$0.33mg/dI, 93.79$\pm$7.06$\mu$g/dI and 98.57$\pm$7.06 $\mu$g/dI and 4.93$\pm$0.62$\mu$g/dI respecitively. 3)Breast fed infants had significantly higher serum calcim, magnesium, sodium and iron concentrations than the formula fed groups. Otherwise, serum potassium, copper and manganese levels in breast milk fed infants were significantly lower than those in formula fed infants. 4) In formula fed infants, serum potassium and copper levels increased increased significantly with months after birth.

• Clinical and Experimental Pediatrics
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• v.57 no.10
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• pp.445-450
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• 2014

Purpose: Familial hypokalemic periodic paralysis (HOKPP) is an autosomal dominant channelopathy characterized by episodic attacks of muscle weakness and hypokalemia. Mutations in the calcium channel gene, CACNA1S, or the sodium channel gene, SCN4A, have been found to be responsible for HOKPP; however, the mechanism that causes hypokalemia remains to be determined. The aim of this study was to improve the understanding of this mechanism by investigating the expression of calcium-activated potassium ($K_{Ca}$) channel genes in HOKPP patients. Methods: We measured the intracellular calcium concentration with fura-2-acetoxymethyl ester in skeletal muscle cells of HOKPP patients and healthy individuals. We examined the mRNA and protein expression of KCa channel genes (KCNMA1, KCNN1, KCNN2, KCNN3, and KCNN4) in both cell types. Results: Patient cells exhibited higher cytosolic calcium levels than normal cells. Quantitative reverse transcription polymerase chain reaction analysis showed that the mRNA levels of the $K_{Ca}$ channel genes did not significantly differ between patient and normal cells. However, western blot analysis showed that protein levels of the KCNMA1 gene, which encodes $K_{Ca}$1.1 channels (also called big potassium channels), were significantly lower in the membrane fraction and higher in the cytosolic fraction of patient cells than normal cells. When patient cells were exposed to 50 mM potassium buffer, which was used to induce depolarization, the altered subcellular distribution of BK channels remained unchanged. Conclusion: These findings suggest a novel mechanism for the development of hypokalemia and paralysis in HOKPP and demonstrate a connection between disease-associated mutations in calcium/sodium channels and pathogenic changes in nonmutant potassium channels.

• Asian-Australasian Journal of Animal Sciences
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• v.1 no.1
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• pp.33-41
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• 1988

In a study about minerals cycling in grassland agro-ecosystem, investigation on relations among two minerals, potassium(K) and magnesium(Mg), and nitrogen(N) was performed. Four kinds of diets different in K and N levels were fed to four goats with a Latin-square method and $2{\times}2$ factorial design. As the basal diet, press cake silage prepared from Italian ryegrass was used because of its uniformity and comparatively low mineral concentrations. Supplementation of K and N were made using potassium bicarbonate and urea. In the experiment, it was clearly shown that high K concentration in the forage crops is the main reason of the low utilization of Mg in ruminant animals. However, high nitrogen intake resulted in the increase of magnesium retention, urinary potassium excretion, water intake and volume of urine and in the decreases of potassium intake minus urinary potassium excretion. The results of high nitrogen intake seemed to be produced in the following order;increase of urine, increase of water intake, increase of urinary potassium excretion, and decrease of intake minus urinary potassium excretion. The amount of potassium intake minus urinary potassium excretion had significantly close relationships with magnesium utilization and serum magnesium concentration. As a conclusion, higher nitrogen intake by ruminants seemed to be preferable for magnesium utilization through increased water intake and urinary potassium excretion, if the sufficient drinking water could be supplied to ruminants.

• Food Science of Animal Resources
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• v.33 no.4
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• pp.549-554
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• 2013

Food safety is important issue for consumers and recently the usage of food ingredients especially food preservatives are limited by regulations. However, some manufacturers use food preservatives instead of improving their hygienic production. Therefore, the levels of sodium benzoate and potassium sorbate of 147 vacuum packaged Kashar (fresh) cheese samples produced in Black Sea Region, Turkey were investigated and some microbiological properties were determined. Research results demonstrated that the production of vacuumed Kashar cheese in Black Sea Region was not standardized for all production periods depending on the microbiological properties. Coliform and E. coli counts detected in the cheese samples showed that necessary hygienic conditions were not provided for Kashar cheese production. Staphylococcus aureus was not determined in the cheese samples. The sodium benzoate and potassium sorbate were analyzed by HPLC-DAD. Potassium sorbate levels (69.39 mg/kg) of Kashar cheese samples were determined to be lower than the maximum permitted concentration of Turkish Food Codex. Although the utilization of sodium benzoate is prohibited by the Codex, the average level of sodium benzoate of cheese samples was detected to be 68.63 mg/kg. Sodium benzoate can be naturally occurred in fresh cheese at concentrations of up to 50 mg/kg.

• Journal of Nutrition and Health
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• v.52 no.3
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• pp.277-284
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• 2019

Purpose: Sodium intake is known to be a critical dietary factor in several diseases including cataract. Earlier studies have reported that excess intake of sodium may elevate the risk of cataract. However, little is known about this in Koreans. Thus, the purpose of this study was to examine whether dietary intake of sodium and potassium might modify the risk of cataract. Methods: A total of 1,319 males (219 cases) and 1,966 females (369 cases) from Korean National Health and Nutrition Examination Survey 2012 were analyzed. Energy adjusted dietary intakes of sodium and potassium and their ratios were evaluated to ascertain their associations with the risk of cataract. Dietary intake levels were stratified into quartiles and their risk modifying effects were estimated with logistic regression models with or without subjects' socio-economic characteristics and life styles for each sex. Results: Findings suggested that various descriptive factors were associated with the risk of cataract either in males or females. Males' intake levels of sodium and potassium and their ratios did not differ between phenotypes. Higher intakes or higher ratio was not associated with the risk of cataract. In contrast, female controls had higher intakes of sodium and potassium. Higher intake of potassium reduced the risk of cataract in females. However, such association was not retained when subjects' socioeconomic status and life styles were factored into the analysis. Conclusion: Dietary sodium and potassium intakes minimally affected the risk of cataract in Korean males and females. More studies are needed to ascertain the true pathological effect of sodium intake on cataract aetiology.

• Journal of Nutrition and Health
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• v.26 no.9
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• pp.1098-1109
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• 1993

The purpose of this study was to investigate macro-mineral contents in human milk longitudinally from 2 days to 12 weeks postpartum. Milk samples were collected from 34 healthy lactating women. Calcium, Phosphorus, magnesium, sodium and potassium were analyzed. Concentrations of the minerals except for magnesium differed significantly within the lactation period. The levels of sodium and potassium declined linearly over the first 12 weeks of lactation, but that of phosphorus increased. Statistically significant quadratic trends were observed in phosphorus and sodium concentrations. There was no significant correlation between maternal age and mineral concentrations throughout the lactations period. In addition, no significant differences were found between the primipara and the multipara for mineral concentrations in human milk. Mineral intake of lactating women did not affect the individual mineral concentrations of milk. However, the protein and fat intake of lactating women had a significant correlation with sodium and potassium concentrations. The following four pairs of minerals : potassium and calcium, potassium and phosphorus, sodium and magnesium, and calcium and phosphorus showed a positive correlation with each other in human milk.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.8
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• pp.1037-1041
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• 2009

The effect of dietary zinc levels on macro mineral contents was investigated in rats. Rats were divided into three groups by zinc levels, such as low (50% of requirement, Zn/L), adequate (100%, Zn/A) and high (200%, Zn/H) levels of dietary zinc. The calcium, magnesium, sodium and potassium contents in serum and organs were measured. The food intake was significantly higher in Zn/L group compared to that of Zn/H group. The body weight gain in Zn/H group was significantly lower than that of other two groups. There were no significant differences in liver, kidney and spleen weights. Also, serum calcium, magnesium, sodium and potassium concentrations were not significantly changed by the zinc diet at any level. In the low zinc diet group, liver calcium content was significantly lower, but the magnesium and potassium contents in spleen were significantly higher than that in other groups. The calcium, magnesium, sodium and potassium contents of kidney were not significantly different for any level of zinc. In conclusion, the macro mineral contents of liver and spleen in the normal rats were changed by zinc levels. Based on this study, the interaction between micro and macro minerals remains to be further studied.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.122-122
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• 2022

In order to investigate changes in rice yield and quality according to phosphate and potassium fertilization levels when nitrogen fertilizer was applied at 7 kg·10a^-1, a field experiment was conducted at National Institute of Crop Science of Korea in 2021. Three Korean rice varieties were grown in paddy fields, and phosphate and potassium fertilizer were treated at three levels(N-P-K 7-4.5-5.7 kg·10a^-1, 7-3-3 kg·10a^-1, 7-0-0 kg·10a^-1). When phosphate and potassium fertilizers were not treated, the yield of Ilpum and Chilbo was significantly reduced, and there was no significant difference in Hopyung. The head rice ratio was significantly lower in the untreated plot of Hopyung and Chilbo, but there was no significant difference in the Ilpum. Protein content was significantly decreased in the untreated plot of Chilbo, and there was no significant difference in other varieties. As a result of this study, it was confirmed that yield, head rice ratio, and protein content were lowered when phosphate and potassium fertilizers were not treated, and the degree of decrease was different depending on the variety.