• 한국해양바이오학회지
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• 제4권1호
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• pp.11-14
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• 2010

The genus Porphyra, consisting of 133 species includes several economically valuable species (i.e. P. yezoensis, P. tenera, P. pseudolinearis etc.). They are predominantly consumed and cultivated in East Asian countries such as Japan, Korea and China, and they are regarded as a big commercial market today. In addition to the industrial importance, P. yezoensis is currently regarded as a feasible candidate for a model plant in marine biotechnology, therefore there are a wide range of studies being undertaken: strain-preservation, development of mutant strains and genetic analysis and exhaustive molecular analysis including EST and macro/micro array. Focusing on the activities of our research group, current situation and future perspectives in marine biotechnological studies using P. yezoensis will be discussed in this mini review.

• 한국양식학회지
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• 제10권3호
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• pp.321-326
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• 1997

The random amplified polymorphic DNA (RAPD) technique was used to anlayze six isolates of two species of Porphyra, P. yezoensis and P. tenera. Four 21-mer prrmers were combined randomly into six groups of double primers and screened for DNA amplification using nuclear and chloroplast tempate DNA. The RAPD patterns resulting from RnRc and CnCc primers provided evidence for both genetically homo-and heterogeneous populations of P. yezoensis and P. tenera. Similarity values obtained by RnRc primer analysis of nuclear DNA varied from 0.364 to 0.714 and those of chloroplast DNA were high, ranging from 0.727 to 1.000, except for P. yezoensis (Enoura).

• 한국해양바이오학회지
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• 제12권1호
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• pp.66-74
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• 2020

Marine algae contain a variety of substances, such as mycosporine-like amino acids, which can defend against UV irradiation. Among them, Porphyra-334 derived from Porphyra yezoensis is attracting attention as a novel active ingredient for anti-aging cosmetics because of its excellent anti-oxidative, anti-inflammatory, and wound-healing properties through promoting skin cell migration. In this study, a process using direct current (DC) for increasing the yield of large-scale purification of Porphyra-334 was developed. When DC was applied to obtain Porphyra-334 efficiently, the purification time was shortened by approximately 1/4 compared with the process wherein DC was not applied; moreover, the yield of purification was improved.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2001년도 춘계 수산관련학회 공동학술대회발표요지집
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• pp.199-200
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• 2001

해조류중 특히 방사무늬 김 (Porphyra yezoensis)의 양식기술은 인공채묘기술이 개발된 이래 양식 및 가공분야의 많은 기술적 진보를 거듭해 오고있으며 양식장은 천해 해역으로 하천수의 유입으로 인한 풍부한 영양염류의 공급을 받고있는 지역에 있다. 이러한 영양염류는 김의 생장촉진에 도움을 주는 반면에 여러 가지 미생물, 미세조류, 부착조류 등의 증식도 함께 촉진하여 결과적으로 김 성장에 대한 영양분의 경쟁관계, 질병유발 김 양식의 생산력 저하등의 문제점들을 동시에 유발하고 있다. (중략)

• 생명과학회지
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• 제29권2호
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• pp.152-157
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• 2019

김(Porphyra yezoensis)은 김속의 홍조류이다. RAPD (random amplified polymorphic DNA) 마커를 이용하여 한국 내 네 집단의 표현형과 유전적 다양성을 조사하였다. 전체적으로 20 시발체로 김에서 55분절이 관찰되었다. 이들 밴드 중 30개(54.5%)는 다형성을 나타내었다. OPA-18-02 밴드는 낙동 김 집단에서만 증폭되었다. OPA-20-02 밴드는 서천 김 집단에서만 증폭되었다. 이 두 밴드는 특별한 집단을 구별해주는 특이밴드로 판정되었다. 대립유전자좌위의 수(Ae)는 1.161에서 1.293로 평균은 1.366였다. 서천 김 집단이 가장 높은 다형성을 나타내었다(0.163). 다른 집단과 격리되고 조간대에 위치한 낙동 김 집단은 가장 낮은 다형성을 나타내었다(0.092). 샤논의 표현형 다양성(I)은 서천 김 집단이 가장 높았다(0.238). 전체 유전적 다양도($H_T$)는 0.132(OPA-02)에서 0.420(OPA-19)로 나타났다. 대립유전자좌위에서 유전적 다양성($H_S$)은 0.059(OPA-18)에서 0.339(OPA-19)였다. 대립유전자좌위에 근거에서 전체 유전적 다양도에서 집단 간 차이($G_{ST}$)는 0.012(OPA-11)에서 0.762(OPA-18)이였으며 평균은 0.415였다. 이는 전체 변이의 약 42%는 집단 간에서 발견된다는 것을 의미한다. 종 내 다양도의 58.5%는 집단 내에 있었다. 유전자 흐름(Nm)은 0.705로 낮았다.

• 한국수산과학회지
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• 제36권3호
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• pp.220-224
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• 2003

Effect of the ingestion of porphyran extracted from Porphyra yezoensis on calcium, magnesium and potassium contents in serum and tissue was investigated using hyperlipidemic and hypercholesterolemic rats. The contents of calcium magnesium and potassium in serum of rats fed porphyran for four weeks were higher than those of the control group. The contents of calcium and potassium were decreased with increasing a porphyran level while magnesium content was increased. Liver calcium contents in an $1\%$ porphyran group were significantly (p<0.05) higher than those in the control group, and magnesium contents in a $10\%$ porphyran group were higher than those in the control group. Kidney calcium and magnesium contents in rats fed porphyran were significantly (p<0.05) high compared with the control group. However, potassium content in kidney was Increased as a porphyran level was increased in diet. Spleen calcium and potassium contents were significantly lower in the porphyran groups than those in the control group. Rats fed the $5\%$ porphyran diet had higher magnesium content in spleen than any other diets. The results showed that diets supplemented with several porphyran levels had variable effects on the contents of calcium, magnesium and potassium in serum and tissue of hyperlipidemic rats.

• ALGAE
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• 제33권1호
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• pp.49-54
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• 2018

Red algal mitochondrial genomes (mtDNAs) can provide useful information on species identification. mtDNAs of Pyropia / Porphyra (Bangiales, Rhodophyta) have shown diverse variation in their size and gene structure. In particular, the introns and intronic open reading frames found in the ribosomal RNA large subunit gene (rnl) and cytochrome c oxidase subunit 1 gene (cox1) significantly vary the mitochondrial genome size in Pyropia / Porphyra species. In this study, we examined the exon / intron structure of rnl and cox1 genes of Pyropia yezoensis at the intraspecific level. The combined data of rnl and cox1 genes exhibited 12 genotypes for 40 P. yezoensis strains, based on the existence of introns. These genotypes were more effective to identify P. yezoensis strains in comparison to the traditional DNA barcode cox1 marker (5 haplotypes). Therefore, the variation in gene structure of rnl and cox1 can be a novel molecular marker to discriminate the strains of Pyropia species.

• 한국수산과학회지
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• 제35권6호
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• pp.633-638
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• 2002

Nuclear 18S ribosomal RNA gene (185 rDNA) from the aquaculturable seaweed Porphya yezoensis (Bangiales, Rhodophyta) was amplified using the polymerase chain reaction and its sequence was analysed. Complete 185 rDNA has an 1823 bp exon and a 514 bp intron. The G+ C contents of exon and intron were $48\%$ and $51.4\%$, respectively. The exon sequence showed $99.5\%$ homology to the GenBank accession number AB013177 of the Japanese p. yezoensis. The intron region that was inserted upstream between 568 and 1083 showed $93.4\%$ homology to the AB013177.

• 환경생물
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• 제30권3호
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• pp.200-209
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• 2012

서식 환경에 따른 양식 김의 종류 및 광합성 색소 조성과 농도에 대하여 2011년 1월부터 3월까지 김 양식이 이루어지고 있는 남서해역에서 조사를 하였다. 수온의 범위는 $3.0{\sim}11.3^{\circ}C$이었고, 염분의 범위는 32.7~34.7으로 연안과 가까운 정점 1과 6에서 낮게 나타났다. 해수 중 DIN의 농도 범위는 $1.73{\sim}12.84{\mu}M$, DIP의 농도 범위는 $0.07{\sim}0.67{\mu}M$이었고, 규산염의 농도 범위는 $4.93{\sim}18.29{\mu}M$이었다. 해수 중 Chl a 농도 범위는 $0.41{\sim}9.14{\mu}g\;L^{-1}$로서 1월의 정점 1에서 가장 높았다. 해수 중 조류색소 조성은 규조류 (diatoms)의 marker 색소인 fucoxanthin이 $0.06{\sim}3.41{\mu}g\;L^{-1}$로 우점하였고, 정점 1에서 $3.41{\mu}g\;L^{-1}$로 1월에 가장 높았다. 용존 무기질소 농도는 모든 정점에서 낮은 염분을 보이는 1월에 높았으나, 정점 1은 용존 무기질소 농도는 낮았다. 김 엽체 색소 중 Chl a, PE 및 PC의 농도는 각각 $1,173{\sim}8,124{\mu}g\;DW\;g^{-1}$, $3,281{\sim}10,076{\mu}g\;DW\;g^{-1}$, $388{\sim}1,346{\mu}g\;DW\;g^{-1}$로서 조사 정점 2, 3에서 높은 농도를 보였다. 김 엽체의 색소농도는 지주식인 모무늬돌김과 방사무늬김이 혼재되어 양식되는 정점 1과 6보다 부류식인 방사무늬김만 생산되는 정점에서 높았고, 방사무늬김만 생산되는 정점에서는 정점 2와 3에서 높았다. 따라서, 김 엽체의 색소 농도는 양식 방법과 해양 환경적 요인에 의하여 달라질 수 있음을 알 수 있었다.

• 한국수산과학회지
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• 제41권6호
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• pp.409-413
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• 2008

A simple method for the purification of porphyran from laver Porphyra yezoensis was developed to obtain information for the development of food materials with biological functionality. Crude porphyran (CP) was extracted from dried laver in boiling water for 3 h, and then fractionated using cetylpyridinium chloride into an acidic fraction (CP-F1) and a neutral fraction (CP-F2). CP-F1 was fractionated further by fractional ethanol precipitation. Fraction CP-F1-70, precipitated at an ethanol concentration of 61-70% was the major fraction containing 68.1% of the yield from the initial fraction CP-F1. The CP-F1-70 fraction displayed a single band on Sepharose CL-4B with a molecular mass of 550 kDa, indicating a homogeneous polysaccharide. The molar ratio of galactose, 3,6-anhydro-L-galactose, 6-0-methyl-D-galactose and ester sulfate of CP-F1-70 was 1:0.32:0.07:0.53. This method is very useful for rapid and large-scale preparation of purified porphyran because it is compatible with mass production.