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http://dx.doi.org/10.5657/kfas.2002.35.6.633

Sequence Analysis of Nuclear 18S rDNA from the Seaweed Porphyra yezoensis (Rhodophyta) in Korea  

Jin Long Guo (Department of Biotechnology, Pukyong National University)
Kim Myung Sook (Department of Biotechnology, Pukyong National University)
Choi Jae Suk (Department of Biotechnology, Pukyong National University)
Cho Ji Young (Department of Biotechnology, Pukyong National University)
Jin Hyung Joo (Department of Biotechnology, Pukyong National University)
Hong Yong Ki (Department of Biotechnology, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.35, no.6, 2002 , pp. 633-638 More about this Journal
Abstract
Nuclear 18S ribosomal RNA gene (185 rDNA) from the aquaculturable seaweed Porphya yezoensis (Bangiales, Rhodophyta) was amplified using the polymerase chain reaction and its sequence was analysed. Complete 185 rDNA has an 1823 bp exon and a 514 bp intron. The G+ C contents of exon and intron were $48\%$ and $51.4\%$, respectively. The exon sequence showed $99.5\%$ homology to the GenBank accession number AB013177 of the Japanese p. yezoensis. The intron region that was inserted upstream between 568 and 1083 showed $93.4\%$ homology to the AB013177.
Keywords
18S rDNA; Exon; Intron; Polphyra yezoensis; Seaweed; Sequence analysis;
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1 White, T.J., T. Bruns, S. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications. Innis, M., J. Gelfand, J. Sninsky, and T. White eds., Academic Press, Florida, 315-322
2 Williams, S.M., RW. DeBry and J. Feder. 1988. A commentary on the use of ribosomal DNA in systematic studies. Syst. Zool., 37, 60-62   DOI   ScienceOn
3 Jin, L.G., M.S. Kim, Y.D. Kim, H.G. Kim and Y.K. Hong. 2001. Sequence Analysis of Nuclear 18S rDNA from Porphyra pseudotineans (Rhodophyta) in korea. Algae, 16, 277-282
4 Kang, J.W. 1970. Species of cultivated Porphyra in Korea. J. Kor. Fish. Soc., 3, 77-92   DOI
5 Kunimoto, M., H. Kito, Y. Kaminishi, Y. Mizukami and N. Murase 1999. Molecular divergence of the ssu rRNA gene and internal transcribed spacer 1 in Porphyra yezoensis (Rhodophyta). J. Appl. Phycol., 11, 211-216   DOI   ScienceOn
6 Kurogi, M. 1972. Systematics of Porphyra in Japan. In: Contributions to the Systematics of Benthic Marine Algae of the North Pacific. Abbott, I. A. and Kurogi, M. eds. Jap. Soc. Phycol., Kobe, 167-191
7 Lee, I.K. and J.W. Kang. 1986. A check list of marine alage in Korea. Kor. J. Phycol., 1, 311-325
8 Lindstrom, S.C. and K.M. Cole. 1992. Relationships between some North Atlantic and North Pacific species of Porphyra (Bangiales, Rhodophyta): Evidence from isozymes, morphology, and chromosomes. Can. J. Bot, 70, 2066-2075   DOI
9 Nicholas, K.B., H.B. Nicholas Jr and D.W. Deerfield. 1997. GeneDoc: Analysis and visualization of genetic variation. EMBNEW News, 4, 14
10 Oliveira, M.C, J. Kumiawan, CJ. Bird, E.L. Rice, CA. Murphy, R.K. Singh, R.R. Gutell and MA Ragan. 1995. A preliminary investigation of the order Bangiales (Bangiophycidae, Rhodophyta) based on sequences of nuclear small-subunit ribosomal RNA genes. Phycol. Res., 43, 71-79   DOI
11 Park, J.W., Y.C. Cho, B.H. Nam, H.J. Jin, C.H. Sohn and Y.K. Hong. 1998. RAPD identification of genetic variation in the seaweed Hizikia fusiformis. J. Mar. Biotechnol., 6, 62-64
12 Brodie, J., P.K. Hayes, G.L. Barker and L.M. Irvine. 1996. Molecular and morphological characters distinguishing two Porphyra species (Rhodophyta, Bangiophycidae). Eur. J. Phycol., 31, 303-308   DOI   ScienceOn
13 Brodie, J., P.K. Hayes, G.L. Barker, L.M. Irvine and I. Bartsch. 1998. A reappraisal of Porphyra and Bangia (Bangiophycidae, Rhodophyta) in the northeast Atlantic based on the rbcL-rbcS intergenic spacer. J. Phycol., 34, 1069-1074   DOI   ScienceOn
14 Dutcher, J.A. and D.F. Kapraun. 1994. Random amplified polymorphic DNA (RAPD) identification of genetic variation in three species of Porphyra (Bangiales, Rhodophyta). J. Appl. Phycol., 6, 267-273   DOI   ScienceOn
15 Hendriks, L., R. De Baere, Y. Van De Peer, J. Neefs, A. Goris and R. Dewatcher. 1991. The evolutionary position of the rhodophyte Porphyra umbilicalis and the basidiomycete Leucosporidium scottii among other eukaryotes as deduced from complete sequences of small ribosomal subunit RNA. J. Mol. Evol., 32, 167-177   DOI
16 Hillis, D.M. and M.T. Dixon. 1991. Ribosomal DNA: Molecular evolution and phylogenetic inference. Quart. Rev. Biol., 66, 411-453   DOI   ScienceOn
17 Jeannmougin, F., J.D. Thompson, M. Gouy, D.G. Higgins and T.J. Gibson. 1998. Multiple sequence alignment with Clustal X. Trends Biochem. Sci., 23, 403-405   DOI   ScienceOn
18 Hong, Y.K., S.D. Kim, M. Polne-Fuller and A. Gibor. 1995. DNA extraction conditions from Poyphyra perforata using LiCl. J. Appl. Phycol., 7, 101-107   DOI   ScienceOn
19 Hwang, M.S., M.H. Han and I.K. Lee. 1998. Allozyme variation and species relationships in the genus Porphyra (Bangiales, Rhodophyta) from Korea. Algae, 13, 447-459
20 Hwang, M.S. and I.K. Lee. 2001. Taxonomy of the Genus Porphyra (Bangiales, Rhodophyta) from Korea. Algae, 16, 233-273
21 Shin, J.A., N. Morikawa, H. Akita and A. Miura. 1996. Polymerase chain reaction-based identification of varieties in Porphyra yezoensis (Bangiales, Rhodophyta). J. Aomori Univ. & Aomori Jr. Coll., 18, 101-106
22 Altschul, S.F., W. Gish, W. Miller, E.W. Myers and D.J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol., 215, 403-410   DOI
23 Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman. 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nuc. Acids Res., 25, 3389-3402   DOI   ScienceOn
24 Araki, S., T. Sakurai, T. Oohusa and N. Sato. 1992. Comparative restriction endonuclease analysis of rhodoplast DNA from different species of Porphyra. Nippon Suisan Gakkaishi, 58, 477-480   DOI
25 Yamazald, S., Y. Kitade, T. Maruyama and N. Saga. 1996. Phylogenetic position of Porphyra yezoensis (Bangiales, Rhodophyta) based on the 18S rDNA sequence. J. Mar. Biotechnol., 4, 230-232
26 해양수산부. 2001. 해양수산 통계 연보. 크리홍보(주), 서울, 1439
27 Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, New York, 18.88
28 Saunders, G.W. and L.D. Druehl. 1992. Nucleotide sequences of the small-subunit ribosomal RNA genes from selected Laminariales (Phaeophyta): Implications for kelp evolution. J. Phycol., 28, 544-549   DOI
29 Stiller, J.W. and J.R. Waaland. 1993. Molecular analysis revealscryp-tic diversity in Porphyra (Rhodophyta). J. Phycol., 29, 506-517   DOI   ScienceOn
30 Tan, I.H. and L.D. Druehl. 1996. A ribosomal DNA phylogeny supports the close evolutionary relationships among the Sporochnales, Desmarestiales, and Laminanales (Phaeophyceae). J. Phycol., 32, 112-118   DOI
31 Van de Peer, Y. and R. De Wachter. 1994. TREECON for Windows: A software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput. App. Biosci.. 10, 569-570
32 Van Oppen, M.J., J.L. Olsen and W.T. Stam. 1993. Evidence for independent acquisition of group I introns in green algae. Mol. Biol. Evol., 10, 1317-1326