• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.4
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• pp.506-515
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• 2015

In this study, the effect of Goheung pomegranate extract on postmenopausal syndrome was evaluated both in vitro and in vivo in ovariectomized Sprague-Dawly (SD) rats. Sixty female SD rats were divided into six groups: sham, sham operation and distilled water; OVX, ovariectomized and distilled water; PE1, ovariectomized and pomegranate concentrate (0.75 mL/twice/d); PE2, ovariectomized and pomegranate concentrate (1.5 mL/twice/d); PE3, ovariectomized and pomegranate concentrate (2.2 mL/twice/d); and CE, ovariectomized and commercial pomegranate concentrate (2.2 mL/twice/d). Percent bone volume (bone volume/tissue volume) and trabecular thickness (Tb.Th) improved in a dose-independent manner in PE1, 2, and 3. Especially, bone mineral density was significantly improved in PE3 (P<0.05) compared to OVX. Pomegranate extract reduced body weight and visceral fat mass. High density lipoprotein cholesterol (HDL-C) level slightly increased in a dose-independent manner in the experimental group. In addition, HDL-C/total cholesterol level of PE3 significantly increased (P<0.05) compared with OVX. These results show that pomegranate concentrate improved blood lipid levels and bone metabolism in ovariectomized rats. Therefore, Goheung pomegranate concentrates are expected to improve cardiovascular and bone-related diseases in menopausal women.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.3
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• pp.1119-1122
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• 2016

Background: Punica granatum (PG) has been demonstrated to possess antitumor effects on various types of cancer cells. In this study, we determined antiproliferative properties of a seed extract of PG (PSE) from Iran in different human cancer cells. Materials and Methods: A methanolic extract of pomegranate seeds was prepared. Total phenolic content (TPC) and total flavonoid content (TFC) were assessed by colorimetric assays. Antioxidant activity was determined with reference to DPPH radical scavenging activity. The cytotoxicity of different doses of PSE (0, 5, 20, 100, 250, 500, $1000{\mu}g/ml$) was evaluated by MTT assays with A549 (lung non small cell carcinoma), MCF-7 (breast adenocarcinoma), SKOV3 (ovarian cancer cells), and PC-3 (prostate adenocarcinoma) cells. Results: Significant (P<0.01) or very significant (P<0.0001) differences were observed in comparison to negative controls at all tested doses ($5-1000{\mu}g/ml$). In all studied cancer cells, PSE reduced the cell viability to values below 23%, even at the lowest doses. In all cases, IC50 was determined at doses below $5{\mu}g/ml$. In this regard, SKOV3 ovarian cancer cells were the most responsive to antiproliferative effects of PSE with a maximum mean growth inhibition of 86.8% vs. 82.8%, 81.4% and 80.0% in MCF-7, PC-3 and A549 cells, respectively. Conclusions: Low doses of PSE exert potent antiproliferative effects on different human cancer cells SKOV3 ovarian cancer cells as most and A549 cells ar least responsive regarding cytotoxic effects. However, the mechanisms of action need to be addressed.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2012.03a
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• pp.55-55
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• 2012

To improve the deodorizing and antibacterial performance of various fabrics (cotton, silk and wool) dyed with pomegranate(Punica granatum L.) extract without mordants, natural colorant solutions, which were extracted from pomegranate using water as an extractant at $90^{\circ}C$ for 90 min with a various liquor ratio (solid natural colorant material/solvent water, weight ratio) from 1:100 to 1:5 were used. To achieve the highest K/S and the deodorizing and antibacterial performance, the best liquor ratio, dyeing bath ratio, dyeing temperature and dyeing time were found to be 1:10, 1:50, $80^{\circ}C$ and 60 min, respectively. The deodorizing performance of dyed cotton, silk and wool fabrics against acetic acid vapor were found to be95,70,90%,respectively. However, all the dyed fabrics displayed outstanding deodorizing performance(99%) against ammonia gas and antibacterial performance(bacteriostatic reduction rate:99.9%) against Staphylococcu aureus and Klebsiella pneumonia(bacteriostatic reduction rate: 99.9%). It is worth noting that pomegranate (Punica granatum L.) colorants notably enhanced the deodorizing and antibacterial performance of cotton, silk and wool fabrics.

• Preventive Nutrition and Food Science
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• v.21 no.1
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• pp.14-23
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• 2016

Non-alcoholic fatty liver disease (NAFLD) is caused by fat accumulation and is associated with oxidative stress. In this study, we investigated the potential protective effect of pomegranate (Punica granatum L.) peel extract (PPE) against oxidative stress in the liver of rats with NAFLD. Sprague-Dawley rats were fed a high fat diet (HFD), 20% corn oil, or palm oil for 8 weeks in the presence or absence of PPE. The control group was fed a basal diet. The progression of NAFLD was evaluated histologically and by measuring liver enzymes (alanine transaminase and aspartate transaminase), serum lipids (triglycerides and total cholesterol), and oxidative stress markers. The HFD feeding increased the body weight and caused NAFLD, liver steatosis, hyperlipidemia, oxidative stress, and elevated liver enzymes. Administration of PPE ameliorated the hepatic morphology, reduced body weight, improved liver enzymes, and inhibited lipogenesis. Furthermore, PPE enhanced the cellular redox status in the liver tissue of rats with NAFLD. Our findings suggest that PPE could improve HFD-induced NAFLD via abolishment of hepatic oxidative damage and hyperlipidemia. PPE might be considered as a potential lead material in the treatment of NAFLD and obesity through the modulation of lipid metabolism.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.8
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• pp.4051-4055
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• 2012

Functional foods include antioxidant nutrients which may protect against many human chronic diseases by combating reactive oxygen species (ROS) generation. The purpose of the present study was to investigate the protective effect of pomegranate peel extract (PPE) on azoxymethane (AOM)-induced colon tumors in rats as an in vivo experimental model. Forty Sprague-Dawley rats (4 weeks old) were randomly divided into 4 groups containing 10 rats per group, and were treated with either AOM, PPE, or PPE plus AOM or injected with 0.9% physiological saline solution as a control. At 8 weeks of age, the rats in the AOM and PPE plus AOM groups were injected with 15 mg AOM/kg body weight, once a week for two weeks. After the last AOM injection, the rats were continuously fed ad-libitum their specific diets for another 6 weeks. At the end of the experiment (i.e. at the age of 4 months), all rats were killed and the colon tissues were examined microscopically for lesions suspected of being preneoplastic lesions or tumors as well as for biochemical measurement of oxidative stress indices. The results revealed a lower incidence of aberrant crypt foci in the PPE plus AOM administered group as compared to the AOM group. In addition, PPE blocked the AOM-induced impairment of biochemical indicators of oxidative stress in the examined colonic tissue homogenates. The results suggest that PPE can partially inhibit the development of colonic premalignant lesions in an AOM-induced colorectal carcinogenesis model, by abrogating oxidative stress and improving the redox status of colonic cells.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.2
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• pp.287-290
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• 2015

Antifungal activities of pomegranate were investigated. Seventy percent ethanol extracts of seeds, peels, and whole fruits of pomegranate showed similar antifungal activities against Candida albicans in liquid media, whereas extract of whole fruit showed relatively high antifungal activity in solid media. When 70% ethanol extracts were fractionated sequentially with chloroform and ethyl acetate, ethyl acetate fraction exhibited the highest anti-fungal activities against C. albicans. Ethyl acetate fractions of whole fruits and peel portions showed at least 36% and 25% growth against C. albicans, Candida glabrata, Candida tropicalis, and Candida lusitaniae in liquid media, respectively. These results indicate that pomegranate contains antifungal compounds soluble with organic solvents.

• Korean journal of food and cookery science
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• v.21 no.2 s.86
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• pp.171-179
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• 2005

This study was investigated on antioxidative and antimicrobial activities of PSW(pomegranate seed water extract), PSE(pomegranate seed ethanol extract) and PSO(pomegranate seed oil). The extraction yields of PSW, PSE and PSO were 28.9, 13.0 and $4.9\%$, respectively. Total phenol contents of PSW, PSE and PSO were 47, 78 mg/g(dry basis) and 40 mg/g, respectively. Electron donating abilities of PSW, PSE and PSO at 1,000 ppm were 18.8, 28.5 and $9.7\%$, respectively. Antioxidative activities in Iinoleic acid substrates at 500 ppm were in order of PSE > $\alpha-tocopherol$ > PSW > PSO. Antioxidative activities in Iinoleic acid emulsion substrates at 200 ppm were in order of $\alpha-tocopherol$l > PSE > PSW > PSO. In antimicrobial activity, PSO showed growth inhibition effect against Micrococcus luteus, Salmonella enteritidis and PSW showed growth inhibition effect against Bacillus cereus, Escherichia coli. Whereas antimicrobial activity of PSE was not observed. The nitrite-scavenging abilities of PSW, PSE and PSO at 2,000 ppm were 27.5, 23.7 and $39.6\%$, respectively. And the SOD-like activities of PSW, PSE and PSO at 1,000 ppm were 15.9, 34.9 and $0.10\%$, respectively.

• Journal of the Korean Society of Clothing and Textiles
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• v.25 no.3
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• pp.598-605
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• 2001

The study that has been conducted is the consideration of the durability of dye, antibacterial activity, and deodorizing effects of cotton and silk textiles dyed with a botanical dying material that has been used in various ways over the years as Korean diet and folk remedies, the pomegranate. 1. The results of the K/S value of dying according to the types of mordants of textiles dyed with pomegranate extract the following have been discovered: The increase of K/S value of dying of the silk textiles appear to be higher than that of the cotton textiles. Next, silk textiles that have been treated with FeSO$_4$.7$H_2O$ appear to be at the highest. 2. The results of the color change measurement show a difference of color can be noticed according to the types of mordant. As opposed to those textiles that had been only scured textiles, all of the dyed textiles appeared to turn yellow. 3. Textiles that had been treated with $K_2$Cr$_2$O(sub)7 recorded the highest durability according to the colorfastness to light results. In the case of the cotton textiles colorfastness to washing, all appeared to show exceptional results of 3rd class or higher, excluding the textiles that had been treated FeSO$_4$.7$H_2O$. However silk textiles recorded relatively low classes of below 3rd to 4th class. For the colorfastness to washing of pollution, the results for both the cotton and silk textiles were excellent at class 4-5. The results of colorfastness to perspiration, colorfastness to rubbing, and colorfastness to dry cleaning showed comparatively exceptional results of 3rd to 4th class or higher when it came to dyed textiles. 4. According to the measurements of antibiosis, dyed textiles that had been treated with SnCl$_2$.2$H_2O$ appeared to show an exceptional bacterium decrease of approximately 70%. 5. According to the deodorizing measurements, dyed silk textiles appeared to have a higher deodorizing effect than cotton textiles. As for cotton textiles, those that had been treated with FeSO$_4$.7$H_2O$ and in the case of dyed silk textiles those that had been treated SnCl$_2$.2$H_2O$ recorded the highest deodorizing effect.

• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2003.05a
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• pp.64-64
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• 2003

• Proceedings of the Korean Journal of Food and Nutrition Conference
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• 2002.06a
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• pp.34-34
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• 2002