• 제목/요약/키워드: polymorphic

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Development and characterization of eleven microsatellite markers for a popular pet stag beetle, Dorcus hopei (Coleoptera, Lucanidae) using paired-end Illumina shotgun sequencing

  • Han, Taeman;Kim, Seung-Hyun;Park, In Gyun;Park, Haechul
    • International Journal of Industrial Entomology and Biomaterials
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    • 제35권2호
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    • pp.97-99
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    • 2017
  • Eleven polymorphic microsatellite loci were developed and characterized for Dorcus hopei in this study. The number of alleles varied from 2 to 21. The observed heterozygosity and expected heterozygosity ranged from 0.1058 to 0.9744 and 0.0997 to 0.8941, respectively. Two loci showed low polymorphism, while the rest were highly polymorphic. Six loci deviated from Hardy-Weinberg Equilibrium. The set of markers will provide effective tools for examining the population genetic structures and be helpful for managing wild population in D. hopei.

참외 전장유전체 염기서열 분석 및 SSR 마커 개발 (Whole genome re-sequencing and development of SSR markers in oriental melon)

  • 송운호;정상민
    • Journal of Plant Biotechnology
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    • 제46권2호
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    • pp.71-78
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    • 2019
  • The objective of this study was to use 'Danta PR', NGS (Next Generation Sequencing) technology for genome resequencing to develop polymorphic makers between Chinese oriental melon, 'Hyangseo 1' and Korean oriental melon. From the resequencing data that covered about 81 times of the genome size, 104,357 of SSR motifs and Indel, and 1,092,436 of SNPs were identified. 299 SSR and 307 Indel markers were chosen to cover each chromosome with 25 markers. These markers were subsequently used to identify genotypes of 'Danta PR' BC1 (F1 x 'Danta PR') population and a genetic linkage map was constructed. SSR, Indel, and SNPs identified in this study would be useful as a breeding tool to develop new oriental melon varieties.

A Random Amplified Polymorphic DNA (RAPD) primer to assist the Identification of Panax ginseng in Commercial Ginseng Granule Products

  • Shim, Young-Hoon;Choi, Jung-Ho;Park, Chan-Dong;Lim, Chul-Joo;Kim, Do-Hun;Cho, Jung-Hee;Kim, Hong-Jin
    • 대한약학회:학술대회논문집
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    • 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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    • pp.85.1-85.1
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    • 2003
  • Previously, we found the operon random primer (OP-5A) that is characteristic the genus Panax by randomly amplified polymorphic DNA (RAPD) analysis. However, OP-5A primer is limited to apply on the differentiation of only crude herbal plants. To construct more sensitive and unique primers on the genus Panax, ginseng-specific DNA profile (350 bp) that was amplified by OP-5A primer were inserted in a plasmid vector in the TA cloning method and sequenced. We designed the PCR primers (Forward: 5"-AGGGGTCTTGCTAT AGCGGAAC-3", Reverse: 5"-AGTCTTAATTTCATATTTTCGTATG-3") and identified the unique ginseng band (350 bp) in commercial granule products including ginseng extracts as well as crude ginseng plants by nascent PCR.(omitted)

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