Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2003.10b
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- Pages.85.1-85.1
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- 2003
A Random Amplified Polymorphic DNA (RAPD) primer to assist the Identification of Panax ginseng in Commercial Ginseng Granule Products
- Shim, Young-Hoon (College of Pharmacy, Chung Ang University) ;
- Choi, Jung-Ho (Korea Food & Drug Administratio) ;
- Park, Chan-Dong (Korea Food & Drug Administratio) ;
- Lim, Chul-Joo (Korea Food & Drug Administratio) ;
- Kim, Do-Hun (Korea Food & Drug Administratio) ;
- Cho, Jung-Hee (Korea Food & Drug Administratio) ;
- Kim, Hong-Jin (Korea Food & Drug Administration)
- Published : 2003.10.01
Abstract
Previously, we found the operon random primer (OP-5A) that is characteristic the genus Panax by randomly amplified polymorphic DNA (RAPD) analysis. However, OP-5A primer is limited to apply on the differentiation of only crude herbal plants. To construct more sensitive and unique primers on the genus Panax, ginseng-specific DNA profile (350 bp) that was amplified by OP-5A primer were inserted in a plasmid vector in the TA cloning method and sequenced. We designed the PCR primers (Forward: 5＂-AGGGGTCTTGCTAT AGCGGAAC-3＂, Reverse: 5＂-AGTCTTAATTTCATATTTTCGTATG-3＂) and identified the unique ginseng band (350 bp) in commercial granule products including ginseng extracts as well as crude ginseng plants by nascent PCR.(omitted)
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