Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Whole genome re-sequencing and development of SSR markers in oriental melon

참외 전장유전체 염기서열 분석 및 SSR 마커 개발

  • Song, Woon-Ho (Department of Life Science, Dongguk University-seoul) ;
  • Chung, Sang-Min (Department of Life Science, Dongguk University-seoul)
  • 송운호 (동국대학교 서울 생명과학과) ;
  • 정상민 (동국대학교 서울 생명과학과)
  • Received : 2019.04.09
  • Accepted : 2019.04.16
  • Published : 2019.06.30
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Abstract

The objective of this study was to use 'Danta PR', NGS (Next Generation Sequencing) technology for genome resequencing to develop polymorphic makers between Chinese oriental melon, 'Hyangseo 1' and Korean oriental melon. From the resequencing data that covered about 81 times of the genome size, 104,357 of SSR motifs and Indel, and 1,092,436 of SNPs were identified. 299 SSR and 307 Indel markers were chosen to cover each chromosome with 25 markers. These markers were subsequently used to identify genotypes of 'Danta PR' BC1 (F1 x 'Danta PR') population and a genetic linkage map was constructed. SSR, Indel, and SNPs identified in this study would be useful as a breeding tool to develop new oriental melon varieties.

Keywords

References

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