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http://dx.doi.org/10.5010/JPB.2019.46.2.071

Whole genome re-sequencing and development of SSR markers in oriental melon  

Song, Woon-Ho (Department of Life Science, Dongguk University-seoul)
Chung, Sang-Min (Department of Life Science, Dongguk University-seoul)
Publication Information
Journal of Plant Biotechnology / v.46, no.2, 2019 , pp. 71-78 More about this Journal
Abstract
The objective of this study was to use 'Danta PR', NGS (Next Generation Sequencing) technology for genome resequencing to develop polymorphic makers between Chinese oriental melon, 'Hyangseo 1' and Korean oriental melon. From the resequencing data that covered about 81 times of the genome size, 104,357 of SSR motifs and Indel, and 1,092,436 of SNPs were identified. 299 SSR and 307 Indel markers were chosen to cover each chromosome with 25 markers. These markers were subsequently used to identify genotypes of 'Danta PR' BC1 (F1 x 'Danta PR') population and a genetic linkage map was constructed. SSR, Indel, and SNPs identified in this study would be useful as a breeding tool to develop new oriental melon varieties.
Keywords
Indel; Oriental melon; Polymorphic markers; Resequencing; SSR;
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