• 한국연초학회지
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• 제21권1호
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• pp.17-25
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• 1999

To elucidate the effect of elevated sublethal temperature ($33\pm1^{\circ}C$) on polyamine metabolism and oogenesis, we investigated alterations in the major polyamines and ornithine decarboxylase (ODC) and arginine decarboxylase (ADC), and ovarian development during the pupal-adult development of the tobacco budworm, Helicoverpa assulta. Ovaries ODC activity under the elevated sublethal temperature ($33\pm1^{\circ}C$) were lower than those of the optimal rearing temperature ($25\pm1^{\circ}C$). whereas ovarian ADC activity was consistently higher than the optimal rearing temperature ($25\pm1^{\circ}C$). When the gonads were exposed to the higher temperature, ovarian putrescine showed somewhat suppressed levels throughout development, indicating a relatively high correlationship with the alteration aspects in ODC or ADC activity under elevated sublethal temperature. A somewhat precocious ovary was observed in an early stage of development at $33\pm1^{\circ}C$, but cellular abnormalities occurred in this ovary. The ovary developed under elevated sublethal temperature was observed the inhibitional effect of polyamine metabolism and the abnormal development of ovariole, which seem to be related to the sterility.

• The Korean Journal of Physiology and Pharmacology
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• 제1권4호
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• pp.457-466
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• 1997

The effects of DFMO or/and putrescine on the dexamethasone-induced apoptosis of CEM cells were studied to investigate the role of polyamines in anti-leukemic glucocorticoid action. Dexamethasone- induced apoptosis was preceded by significant decreases of cellular polyamine contents and putrescine uptake activity. But DFMO produced decreases of putrescine and spermidine contents and marked increase of putrescine uptake activity, but did not induce apoptosis. However, dexamethasone and DFMO, respectively, induced $G_1-arrest$ in cell cycle and hypophosphorylation of pRb, resulting in the increase of $G_1$ to S ratio and decrease of CEM cell count. DFMO enhanced the dexamethasone-induced apoptosis and $G_1-arrest$. On the other hand, putrescine little affected the apoptotic and $G_1-arresting$ activities of dexamethasone, but almost suppress the effects of DFMO and also the DFMO-dependent enhancement of dexamethasone effects. These results suggested that the dexamethasone-induced apoptosis to be associated with pRb hypophosphorylation and $G_1-arrest$ in CEM cells might be ascribed to the concomitant decreases of cellular polyamine contents and putrescine uptake activity.

• 분석과학
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• 제8권4호
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• pp.895-900
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• 1995

Urinary free and acetylated polyamine profiles have been investigated for their potential usefulness as biochemical markers of cancer in a control of group comprised of healthy volunteers (32 cases) and patients with various types of cancers(48 cases). The nine (5 free and 4 acetylated) endogeneous polyamines were simultaneously determined by a sensitive capillary gas chromatography/nitrogen-phosphorus detector (GC/NPD). The newly modified (simple and convenient) method was developed and the compounds were isolated by adsorption onto silica gel and derivatized by heptafluorobutyric anhydride to enhance their specificity on gas chromatograms. The good quality-control data were obtained through the precision and accuracy test and the recovery range of them was 48.6 ~ 101.2 %. The Korean reference values of urinary polyamines were established and significant differences were found in cancer patients compared with normal subjects. Also, to eliminate subject variations, precursors to product concentration ratios were compared between cancer patients and control group. The ratios of both putrescine to spermidine and total (free plus acetylated) putrescine to total spermidine were significantly greater in cancer patients than in normal subjects.

• Bulletin of the Korean Chemical Society
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• 제27권1호
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• pp.99-105
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• 2006

Polyamine dendrimers functionalized with electrochemiluminescent (ECL) polypyridyl Ru(II) complexes, dend-$[CO-(CH_2)_3-mbpy{\cdot}Ru(L)_2]_3(PF_6)_6$ (dend: N$(CH_2CH_2NH)_3$-, L: bpy, o-phen, phen-Cl, DTDP), were synthesized through the complexation of dendritic polypyridyl ligands to Ru(II) complexes. Their electrochemical redox potentials, photoluminescence (PL), and relative ECL intensities were studied. The ECL emissions produced by the reaction between the electro-oxidized $Ru^{3+}$ species of polyamine dendrimers and tripropylamine as a coreactant were measured in a static system with potential cycles between 0.8 and 1.3 V or through flow injection analysis with a potential of +1.3 V, and were compared to that of $[Ru(o-phen)_3](PF_6)_2{\cdot}Dend-[CO-(CH_2)_3-mbpy{\cdot}Ru(bpy)_2]_3(PF_6)_6$ showed an ECL intensity that was two-fold greater than that of the reference complex $[Ru(o-phen)_3](PF_6)_2$.

• Journal of Microbiology
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• 제43권5호
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• pp.398-405
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• 2005

Polyamines such as putrescine are small, ubiquitous polycationic molecules that are required for optimal growth of eukaryotic and prokaryotic cells. These molecules have diverse effects on cell physiology and their intracellular content is regulated by de novo synthesis and uptake from the environment. The studies presented here examined the structure of a putative polyamine transporter (Pot) operon in Streptococcus pneumoniae (pneumococcus) and growth of pneumococci in medium containing putrescine substituted for choline. RT-PCR experiments demonstrated that the four genes encoding the Pot system are co-transcribed with murB, a gene involved in an intermediary step of peptidoglycan synthesis. Pneumococci grown in chemically-defined media (CDM) containing putrescine without choline enter logarithmic phase growth after 36-48 hs. However, culture density at stationary phase eventually reaches that of choline-containing medium. Cells grown in CDM-putrescine formed abnormally elongated chains in which the daughter cells failed to separate and the choline-binding protein PspA was no longer cell-associated. Experiments with CDM containing radiolabeled putrescine demonstrated that pneumococci concentrate this polyamine in cell walls. These data suggest that pneumococci can replicate without choline if putrescine is available and this polyamine may substitute for aminoalcohols in the cell wall teichoic acids.

• 분석과학
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• 제12권5호
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• pp.441-446
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• 1999

무우 유식물의 자엽에서 Abscisic acid(ABA). ABA plus cyeloheximide(CH)와 ABA plus polyamine(PA) 처리로 유도되는 아미노산의 변화를 분석하였다. ABA 처리로 봄무우에서는 총 아미노산의 농도가 증가되었고, 겨울무우에서는 감소되었다. 봄무우에서는 친수성 아미노산인 proiine, glycine, serine과 cysteine 등이 증가되었고(특히 proline), 반면 겨울무우에서는 cysteine, leucine와 phenylalanine이 증가되었다(특히 cysteine과 leucine). CH는 ABA가 유도시킨 탈수에 대항할 수 있는 단백질의 합성을 방해하여 아미노산의 축적을 초래하였다. PA처리는 반대로 봄무우에서는 총 아미노산의 농도가 감소되었고, 겨울무우에서는 증가되었다. 이상의 결과는 축적된 친수성 아미노산과 단백질이 스트레스에 대항하여 탈수의 적응을 유도하였고, PA은 ABA에 의한 탈수 스트레스의 적응능력을 조절한다고 본다.

• 생명과학회지
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• 제16권3호
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• pp.522-526
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• 2006

UV-B에 의한 강낭콩 식물의 피해양상, 지질과산화, 지질조성 변화와 UV-B에 대한 식물의 방어기작 등을 조사하기 위하여 3주간 UV-B 조사 실험을 수행하였다. UV-B 처리에 의해 초장이 약 22% 정도 감소하는 것으로 나타났다. MDA 함량은 UV-B 처리에 의해 약 50% 정도 증가하는 것으로 나타났다. Glutathione 및 ascorbate acid 함량은 UV-B 조사에 의해 산화형이 증가하고 환원형이 감소하는 것으로 나타났다. UV-B 조사에 의한 지방산 구성 변화를 조사한 결과 당지질 및 인지질 모두 UV-B조사에 의해 포화지방산이 증가하는 것에 반해, 불포화지방산이 감소하는 것으로 나타났다. 강낭콩 잎에는 주로 3종류의 polyamine이 존재하는 것으로 나타났으며, 3종류 모두 UV-B 조사에 의해 증가하는 것으로 나타났다. 본 실험 결과로 볼 때, UV-B 조사는 활성산소를 생성하여 생체막 지질에 직접적인 영향을 미치는 것으로 나타났으며, ascorbate acid, glutathione, polyamine 등의 항산화물질들이 이에 대한 피해를 최소화하기 위하여 작용하는 것으로 나타났다.

• 한국환경과학회지
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• 제2권4호
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• pp.255-270
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• 1993

녹두 유식물의 자엽에서 엽록소 및 단백질 함량과 $\delta$-aminolevulinate dehydratase(ALAD)활성의 변화에 미치는 polyamine의 영향을 조사하였다. Polyamine은 녹화과정에서 자엽내의 엽록소 생성을 촉진하였으며, 이 효과는 KCl에 의해서 상승되었다. 자엽내의 단백질 함량의 변화 또한 엽록소 함량의 변화와 유사하였다. ALAD활성은 암하에서보다 광선하에서 억제되었으나, 18시간 암처리후의 광조사는 ALAD활성을 증가시켰다. Putre-scine처리에 의한 ALAD활성은 암하에서 촉진효과가 낮았으나 광선하에는 그 활성이 증가되었다. KCl은 암하에서 ALAD활성을 촉진시켰으나 광선하에서는 그 효과가 감소되었다. 또한 polyamine과 KCl의 복합처리에서는 촉진효과가 없었다. 이와같은 결과에서 녹두자엽에서의 색소체발달은 polyamine과 광선에 의해 영향을 받으며, polya- mine은 색소체발달에 중요한 작용을 하는 것으로 사료된다.

• Journal of Microbiology
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• 제40권4호
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• pp.305-312
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• 2002

The effects of two natural polyamines (putrescine and spermidine) on the synthesis of ArcA, a response regulator of the Arc two-component signal transduction system, were studied using an E. coli mutant deficient in polyamine biosynthesis. Endogenous polyamine deficiency of the mutant resulted in marked reduction in the ArcA level determined by Western blot analysis. Putrescine supplement to the growth medium effectively increased the ArcA level of the mutant in a concentration-dependent manner. Spermidine also stimulated the ArcA level in the mutant to a greater degree than putrescine. Expression of arcA'::lacZ operon fusion in the mutant was stimulated 6-fold and 10-fold by putrescine and spermidine at a 1mM concentration, respectively, indicating that the stimulatory effect of the polyamines on ArcA synthesis is due to transcriptional induction, and that spermidine is a more potent arcA inducer than putrescine. The polyamine-dependent arcA'::lacZ induction was growth-phase-dependent and independent of either arcA or fnr which are two regulators involved in anaerobic stimulation of the Arch level. These results suggested that putrescine and spermidine polyamines may be potential intracellular signal molecules in the control of arcA expression, and thereby may play an important role in cellular metabolism.

• Membrane and Water Treatment
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• 제11권2호
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• pp.141-149
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• 2020

Prechlorination could increase the removal efficiency of Synedra, but there was no significant effect of increasing the amount of chlorine added. However, a removal efficiency of greater than 80% was noted when ozone was injected at concentrations greater than 2 mg/L. Also, it was found that on addition of polyamine, a removal efficiency of 80% or more could be achieved. As a result of the analysis of field operation data from the water treatment plants G and B, it was found that at water treatment plant G, the filter run time decreased to 10 hours or less when only coagulant was injected, but the filter run time increased to around 40 hours when polyamine (3 mg/L) was also injected. The Synedra population in the raw water subsequently increased to 2,340 cells/mL, and the filter continued running for more than 20 hours. At water treatment plant B, the average Synedra removal efficiency was 56% when only coagulant was injected, and the filter run time decreased drastically with the increasing population of Synedra in the raw water. However, the removal efficiency of Synedra reached 79% when polyamine was injected together with the coagulant, 90% when ozone was also injected, and 95% when polyamine and ozone were injected together and the filter continued running for over 50 hours. The filter run time was maintained at 60 hours when a Synedra population of 6,890 cells/mL flowed into the Paldang water source, but the filter run time with Synedra at 1,960 cells/mL decreased rapidly from 65 hours to 35 hours when the ratio of the size of the individual Synedra reaching 250 ㎛ or more, increased from 38% to 94%. Therefore, the size of the Synedra is considered to be a factor that significantly influences filter clogging, as well as the size of the Synedra population.