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Polyamine Stimulation of arcA Expression in Escherichia coli  

Rhee, Mun-Su (Department of Biological Science, Sung Kyun Kwan University)
Kim, Young-Sik (Department of Biological Science, Sung Kyun Kwan University)
Park, Seon-Young (Department of Biological Science, Sung Kyun Kwan University)
Park, Myung-Hun (Department of Biological Science, Sung Kyun Kwan University)
Kim, Bo-Min (Department of Biological Science, Sung Kyun Kwan University)
Kang, Seong-Uk (Department of Biological Science, Sung Kyun Kwan University)
Lee, Kui-Joo (Department of Biological Science, Sung Kyun Kwan University)
Lee, Jong-Ho (Department of Biological Science, Sung Kyun Kwan University)
Publication Information
Journal of Microbiology / v.40, no.4, 2002 , pp. 305-312 More about this Journal
Abstract
The effects of two natural polyamines (putrescine and spermidine) on the synthesis of ArcA, a response regulator of the Arc two-component signal transduction system, were studied using an E. coli mutant deficient in polyamine biosynthesis. Endogenous polyamine deficiency of the mutant resulted in marked reduction in the ArcA level determined by Western blot analysis. Putrescine supplement to the growth medium effectively increased the ArcA level of the mutant in a concentration-dependent manner. Spermidine also stimulated the ArcA level in the mutant to a greater degree than putrescine. Expression of arcA'::lacZ operon fusion in the mutant was stimulated 6-fold and 10-fold by putrescine and spermidine at a 1mM concentration, respectively, indicating that the stimulatory effect of the polyamines on ArcA synthesis is due to transcriptional induction, and that spermidine is a more potent arcA inducer than putrescine. The polyamine-dependent arcA'::lacZ induction was growth-phase-dependent and independent of either arcA or fnr which are two regulators involved in anaerobic stimulation of the Arch level. These results suggested that putrescine and spermidine polyamines may be potential intracellular signal molecules in the control of arcA expression, and thereby may play an important role in cellular metabolism.
Keywords
polyamine; putrescine; spermidine; transcriptional regulation; arcA; Escherichia coli;
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  • Reference
1 Purification and phosphorylation of the Are regulatory components of Escherichia coli. /
[ Iuchi,S.;E.C.C.Lin. ] / J.Bacteriol.
2 Iron and oxygen regulation of Escherichia coli MnSOD expression: competition between the global regulators Fur and ArcA for binding to DNA. /
[ Tardat,B.;D.Touati. ] / Mol. Microbiol.   ScienceOn
3 Requirnment for terminal cytochtomes in generation of the aerobic signal for the arc regulatory system in Escherichia coli:study utilizing deletions and lac fusions of cyo and cyd. /
[ Iuchi,S.;V.Chepuri;H.A.Fu;R.B.Gennis;E.C.C.Lin. ] / J. Bacteriol.
4 arcA(dye), a global regulatory gene in Escherichia coli mediating repression of en aerobic pathways. /
[ Iuchi,S.;E.C.C.Lin. ] / Proc. Natl.Acad.Sci.USA   ScienceOn
5 Indirect stimulation of genetic recombination. /
[ Golub,E.I.;K.B.Low. ] / Proc. Natl.Acad.Sci.USA.   ScienceOn
6 Anaerobic activation of arcAtranscription of Escjerichia coli: roles of Fnr and ArcA. /
[ Compan,I;D.Touati. ] / Mol.Microbiol.   ScienceOn
7 Effect of putrescine on induction of Escherichia coli paiAB is mediated through ArcA. /
[ Rhee,M.S.;S.U.Kang;J.H.Lee. ] / 101st American Society for Microbiology Meeting H-80
8 The aerobic/anaerobic interface. /
[ Sawers,G. ] / Curr. Opin.Micobiol.   ScienceOn
9 Identification of conserved, RpoS-dependent stationary-phase genes of Escherichia coli. /
[ Schellhorn,H.E.;J.P.Audia;L.I.C.Wei;L.Chang. ] / J.Bacteriol.
10 Spermidine synthase of Escherichia coli: localization of the speE gene. /
[ Tabor,C.W.;H.Tabor;Q.W.Xie ] / Proc. Natl. Acad. Sci USA   ScienceOn
11 /
[ Miller,J. ] / Experiments in molecular genetics.
12 The Cpx envelope stress response is controlled by amplification and feedback inhibition. /
[ Raivio,T.L.;D.L.Popkin;T.J.Silhavy. ] / J.Bactriol
13 In vitro phosphorylation study of the Arc two component signal transduction system of Escherichia coli. /
[ Georgellis,D.;A.S.Lynch;E.C.C.Lin. ] / J.Bacteriol.
14 Polyamines in microorganisms. /
[ Tabor,C.W.;H.Tabor. ] / Microbiol.Rev.
15 Gene expression profiling of Escherichia coli growth transitions: an expanded stringen response midel. /
[ Chang,D.E.;D.J.Smalley;T.Conway. ] / Mol. Microbiol.   ScienceOn
16 /
[ Hoch,J.A.;T.J.Shihavy. ] / Two-component signal transduction.
17 Multimerixation of phosphorylated and non-phosphorylated ArcA is necessary for the response regulator function fo the Arc two-component signal transdution system. /
[ Jeon,Y;Y.S.Lee;J.S.Han;J.B.Kim;D.S.Hwang. ] / J.Biol.Chem.   ScienceOn
18 Polyamine deficiency reduces the rate of DNA replication fork movement in Escherichia coli. /
[ Geiger,L.E.;D.R.Morris ] / Nature   ScienceOn
19 Protein measurement with tne Folin phenol reagent. /
[ Lowry,O.H.;N.J.Rosebrough;A.L.Farr;R.J.Randall. ] / J. Biol. Chem.
20 Spermidine biosynthesis in Escherichia coli: promoter and termination regions of the speEDoperon. /
[ Xie,Q.W.;C.W.Tabor;H.Tabor. ] / J.Bacteriol.
21 Adapttion of Escherichia coli to redox environments by gene expression. /
[ Iuchi,S.;E.C.C.Lin. ] / Mol. Microbiol.   ScienceOn
22 Celluiar and molecular physiology of Escherichia coli in the adaptation to aerobic environments. /
[ Iuchi,S.;L.Weiner. ] / J.Biochem   ScienceOn
23 1,4-Diaminobutane(Putrescine), spermidine, and spermine. /
[ Tabor,H.;C.W.Tabor. ] / Ann.Rev.Biochem   ScienceOn
24 Improved single and multicopy lac-based cloning vectors for protein and operon fusions. /
[ Simon,R.V.;F.Houman;N.Kleckner. ] / Gene   ScienceOn
25 Polyamines enhancement fo the synthesis fo adenylate cylase at the translational level and the consequential stimulation of the synthesis fo the RNA polymerase σ28 subunit. /
[ Yoshida,M.;K.Kashiwagi,K.G.Kawai;A.Ishihama;K.Igarashi. ] / J. Biol. Chem.   ScienceOn
26 The speE speD operon of Escherichia coli. Formantion and processing of a proenzyme form of Sadenosylmethionine decarboxylase. /
[ Tabor,C.W.;H.Tabor. ] / J. Biol. Chem
27 Effects of high glucose and TGF-betal on the expression of collagen IV and vascular endothelial growth fator in mouse podocytes. /
[ Iglesias-De;La.Cruz MC.;F.N.Ziyadeh;M.Isono;M.Kouahou;D.C.Han;R.Kalluri;P.Mundel;S.Chen. ] / Kidney Int.   ScienceOn
28 /
[ Ausubel,F.M.;R.Brent;R.E.Kingston;D.D.Moore;J.G.Seidman;J.A.Smith;K.Struhl. ] / Short protocols in Molecular Biology.
29 Polyamines as constituents of the outer membranes of Escherichia coli and Salmonella typhimurium. /
[ Koski,P;M.Vaara. ] / J.Bacteriol.
30 /
[ Park,S.J.;P.A.Cotter;R.P.Gunsalus. ] / Autoregulation of the acrA gene Escherichia coli. 92nd American Society for Microbioligy Meeting H-145.
31 Transeriptional control mediated by ArcA two-componment response regylator protein of Escherichia coli:Characterization of DNA binding at target promotors. /
[ Lynch,A.S.;E.C.C.Lim. ] / J. Bacteriol.
32 Biosynthesis of polyamines in ornithine decarboxylase, arginine decarboxylase, and agmatine ureohydrolase deletion mutants of Escherichia coli K-12. /
[ Panagiotidis,C.A.;S.Blackburn;K.B.Low;E.S.Canellakis. ] / Proc. Natl.Acad.Sci.USA.   ScienceOn
33 Polyamine biosynthesis and function in Escherichia coli. /
[ Tabor,H.;C.W.Tabor. ] / Adv.Polyamine Rev.
34 Polyamines /
[ Tabor,H.;C.W.Tabor. ] / Annu. Rev. Biochem.   ScienceOn
35 Quinones as the redox signal for the Arc two-component systam of bacteria. /
[ Georgellis.D.;O.Kwon;E.C.C.Lin. ] / Science
36 On the role of cyclic AMP and the Fnr protein in Escherichia coli growing anaerobically. /
[ Unden,G.;A.Duchene. ] / Arch Microbiol.   ScienceOn
37 Putrescine as an essential growth gactor for a mutant of Aspergillus nidulans. /
[ Sneath,P.H. ] / Nature