• 제목/요약/키워드: polyamine

검색결과 264건 처리시간 0.026초

대두 종자의 유근생장시 Ferulic Acid 가 Polyamine 함량과 효소활성에 미치는 영향 (Effect of Ferulic Acid on Polyamine Titers and Enzyme Activities during the Radicle Growth of Glycine max)

  • Kim, Yong-Ok;Ho-Joon Lee;Young-Dong Cho
    • The Korean Journal of Ecology
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    • 제19권5호
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    • pp.385-392
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    • 1996
  • Changes in polyamine titers and enzyme activities during radicle growth of Glycine max were studied in order to investigate the effect of ferulic acid in regulation of polyamine biosynthesis. Among eight compounds used, gallic acid stimulated the radicle growth and ferulic acid inhibited it significantly. During the radicle growth of Glycine max, the content of putrescine was shown the highest level at the second day, while at the fourth day spermidine was the highest and spermine followed. Ornithine decarboxylass (ODC, EC 4.1.1.17) seems to be responsible for biosynthesis of putrescine. As the concentration of ferulic acid (0.001, 0.01, 0.1 mM) treated increased, the content of spermine was gradually enhanced and putrescine was increased at 0.001~0.01 mM, decreased after 0.1 mM concentration but spermidine was not affected. Ferulic acid elevated ODC and S-adenosylmethionine decarboxylass (SAMDC, EC 4.1.1.50) activity. ODC activity was increased more than 120% and SAMDC activity was increased about 50% more than that of the control. Diamine oxidase (DAO EC, 1.4.3.6) activity was enhanced about 20% at low concentration, decreased after then.

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Polyamine이 쪽 모상근배양에서 인디고 생합성에 미치는 영향 (Effect of Polyamines on Indigo Biosynthesis in Hairy Root Cultures of Polygonum tinctorium Lour.)

  • 김진만;장홍기;박상언;류화원
    • 한국작물학회지
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    • 제51권spc1호
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    • pp.247-250
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    • 2006
  • 본 연구는 polyamine이 쪽 모상근 배양에서 뿌리의 생장과 indigo 생합성에 미치는 영향을 조사하였다. 1. 토양미생물인 Agrobacterium rhizogenes R1000을 이용하여 쪽 자엽으로부터 모상근을 효과적으로 유도하여 배양하였다. 2. 모상근 생장과 인디고 생합성이 polyamines(putrescine, spaermidine, spermine) 처리에 의하여 향상되었으며, 그 중 putrescine이 다른 polyamines에 비하여 효과적이었다. 3. Putrescine 70 mg/l 처리가 모상근의 생육(4.4 g/flask)과 인디고 생합성(216.3 ug/g)의 향상에 최적조건으로 조사되었다.

숙주 배축생장과정 중 스트론티움에 의한 오옥신산화 및 폴리아민 생합성 촉진 (Strontium Stimulates IAA Oxidation and Polyamine Synthesis in Germinating Mung Bean Hypocotyls (Vigna radiata L.))

  • 김태완
    • 한국토양비료학회지
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    • 제37권1호
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    • pp.7-13
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    • 2004
  • 본 연구는 숙주의 발아 과정 중 오옥신 과련 대사작용에서의 strontium의 역할을 구명코자 실시하였다. 오옥신 첨가 용액에 strontium을 첨가하여 발아시킨 결과 침지 한 후 IAA 산화가 일어남이 밝혀졌다. 3-5배의 IAA 산화효소 활성화가 측정되었다. 이러한 결과는 strontium에 의한 생장억제의 직접적인 증거였다. 더 나아가 1-10 mM의 strontium 첨가 용액에서 생장한 배축에서의 spermine과 spermine 함량이 2-3배 높은 것으로 밝혀졌다. 이러한 증가는 생체중에 근거한 결과였다. 결론적으로 strontium에 의한 배축생장 억제는 오옥신 산화 및 polyamine 생합성과 관계가 있는 것으로 밝혀졌다.

금속막을 이용한 저압 막 공기병용 역세척수 처리 타당성 연구 (Experimental study on feasibility test for removing particles in air scouring membrane backwash water with metal membrane)

  • 박노석;윤석민;문용택;이선주;박성혁
    • 상하수도학회지
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    • 제29권2호
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    • pp.251-259
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    • 2015
  • The main objective of this research is to study feasibility for applying metal membrane to remove particles from air scouring membrane backwash water. Also, the research was conducted to investigate the influence of polyamine coagulation on floc growth in membrane backwash water as pretreatment for removal particles. From the results of experiments for evaluating the influence of polyamine coagulation on floc growth, it was investigated that particles in the rage of $2{\sim}50{\mu}m$ grew up to $30{\sim}5,000{\mu}m$. In addition, all six metal membranes showed lower removal efficiency, which was 0.87~13.89%, in the case of no polyamine coagulant. On the other hand, in the case of injecting polyamine coagulant, those did extremely high efficiency in 56~92%. From the SEM(Scanning Electron Microscope) images of filtered wiremesh and metal foam membrane, sieve effects were predominant for liquid solid separation in wiremesh and adsorption and diffusion capture effects were predominant in metal foam membrane.

인삼(Panax ginseng C.A. Meyer) Saponin 성분이 흰쥐의 장기에서 Polyamine 대사에 미치는 영향

  • 최연식;조영동
    • Journal of Ginseng Research
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    • 제20권3호
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    • pp.233-240
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    • 1996
  • In order to study effects of Korean ginseng (Panax ginseng C.A. Meyer) saponin fraction on polyamine metabolism in rat organs, Korean ginseng saponin fraction was administrated to rats for 1, 2, 3, 4, 6 and 12 months and brain, liver, prostate, spleen and testis were removed from these rats. Two enzyme activities were measured from those organs; ornithine decarboxylase (ODC), which is a regulatory enzyme of putrescence biosynthesis and S-adenosylmethionine decarboxylase (SAMDC), which is also a regulatory enzyme of spermidine and spermine biosynthesis. The amounts of polyamine were also determined. As for prostate and testis organs, Korean ginseng saponin fraction was innocuous for ODC and SAMDC activities from rats fed for 1 and 2 months. However, after 3 months, the stimulatory effect on the activities of two enzyme gradually increased in test groups and reached its maximum in 12 months. The contents of spermidine and spermlne of test groups in prostate and testis were also much higher than those of control groups. Another stimulatory effect on the activities of two enzymes was observed in liver and reached its maximum in 6 months. In the other organs such as brain and spleen, the enzymes were turned out to be not affected by feeding Korean ginseng saponin fraction. From the cumulative results, the stimulatory effect of Korean ginseng saponin fraction on polyamine metabolism was observed in prostate, testis and liver.

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미생물 ornithine decarboxylase와 polyamines에 관하여

  • 성창근
    • 미생물과산업
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    • 제19권1호
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    • pp.2-7
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    • 1993
  • polyamine을 생합성하는 율속단계효소인 ornithine decarboxylase(ODC)는 미생물에서 많이 연구되어 왔으며 실제적으로 이 효소에 대한 연구는 세균에서 그 효시를 이루었다(1-5,19,35). 최근 들어서는 Escherichia coli(1-22), Saccharomyces cerevisiae (35-40), Neurospora crassa, Physarum polycephalum에서 보다 상세한 연구가 이루어졌는데, 본 고에서는 Escherichia coli, Saccharomyces cerveisiae에 관하여 좀 더 자세히 살펴보고자 한다. Polyamines의 연구나 ODC효소에 대한 연구는 미생물이 많이 이용되었는데, 이는 비교적 쉽게 세포배양이 되기도 하지만, ODC가 결손된 변이주를 만들기가 비교적 용이한 이유도 있었다.

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생체생장에 관한 세포생물학적 연구 Polyamine과 auxin이$\beta-1,4-endoglucanase$ 활성에 미치는 영향 (Cell Biological Studies on Growth and Development Effect of polyamine and auxin on $\beta-1,4-endoglucanase$)

  • 조영동
    • Journal of Plant Biology
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    • 제31권3호
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    • pp.239-247
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    • 1988
  • Spermidine, spermine and IAA promoted an increase in $\beta$-1, 4-endoglucanase activity in hypocotyls of Glycine max. The optimal concentration for the increase of the enzyme activity was 10-6 M for spermidine, 10-8 M for spermine and 10-6 M for IAA. However, IAA had innocuous effect on arginine decarboxylase and ornithine decarboxylase activites, and the content of polyamine. Such cumulative results suggest that the increase in $\beta$-1, 4-endoglucanase activity by IAA is not attributed by the effect on the biosynthesis of polyamine by IAA but spermidine, spermine and IAA induce cell wall loosening and therefore extension growth of cells.

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당근 Hairy root의 성장 및 Anthocyanin 함량에 미치는 Polyamime의 영향 (Effects of Polyamine on Growth and Anthocyanin Contents of Carrot Hairy Root)

  • 안준철;표병식황백
    • KSBB Journal
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    • 제6권2호
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    • pp.195-199
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    • 1991
  • The effects or polyamines on growth, anthocyanin contents and $\beta$-glucan synthetase(GSII) activity in carrot hairy root were studied. Growth of hairy root was stimulated somewhat when each polyamine concentration was treated, especially addition of 1mM spermidine resulted in about 20% increase. On the whole, the axial diameter of hairy root was increased in response to increase in concentration of polyamine. On the other hand, GSII activity was stimulated in response to increase in concentration of polyamine, especially addition of 1mM spermine resulted in about 100% increase of activity. Therefore increased activity of GSII stimulated growth and thickness of hairy root. Anthocyanin contents were not affected by the polyammine.

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