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Effect of Polyamines on Indigo Biosynthesis in Hairy Root Cultures of Polygonum tinctorium Lour.  

Kim, Jin-Nam (School of Biological Sciences and Technology, Chonnam National University)
Jang, Hong-Gi (SENOCO Co.)
Park, Sang-Un (Division of Plant Science and Resources, Chungnam National University)
Ryu, Hwa-Won (School of Biological Sciences and Technology, Chonnam National University)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.51, no.spc1, 2006 , pp. 247-250 More about this Journal
Abstract
We herein studied the effect of polyamines (putrescine, spermidine and spermine) on growth and indigo biosynthesis in hairy root cultures of Polygonum tinctorium Lour. Our results revealed that polyamine treatment increased hairy root growth and indigo biosynthesisat all tested concentrations, with the highest growth rate (4.4 g/ flask) and indigo yield (216 ug/g) induced by 70 mg/L putrescine. These results show far the first time that the growth rates and indigo biosynthesis of Polygonum tinctorium hairy roots may be improved by addition of polyamines to the liquid culture medium.
Keywords
Agrobacterium rhizogenes; hairy root culture; indigo; polyamine; Polygonum tinctorium Lour.;
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