• Journal of Korean Society of Forest Science
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• v.99 no.3
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• pp.344-352
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• 2010

To investigate the mating system of clones in the seed orchard of Japanese red pine, parameters of mating system, such as outcrossing rates, number of potential pollen contributors, and degree of pollen contamination, were estimated in the seed orchard of Japanese red pines on the basis of DNA data including 4 nSSR and 6 cpSSR markers. Estimates of outcrossing rates were ranged from 94.9 to 100% with an average of 98.9% on the basis of the analysis of cpSSR haplotypes. They were ranged from 90.3% to 100% with an average of 95.9% on the basis of the analysis of nSSR genotypes. However, cross checking of both DNA markers revealed that the seeds presumed to be products of self pollination were turned out to be generated by pollination between mother tree and other tree (i.e., 100% of cumulative outcrossing rate). Estimates of pollen contamination ranged from 43.6% (Gangwon-10) to 56.4% (Gangwon-12) with the average of 48.9%. On the basis of pooled cpSSR haplotype of each seed, maximum number of 21 pollen contributors were verified from the seeds reproduced by Kyungbuk-38. Minimum number of 13 pollen contributors were verified in Gangwon-10. Mean of 16.2 pollen contributors were verified from a total of 5 mother trees. In conclusion, considering pretty high outcrossing rates between clones within a seed orchard, it may be expected that a fairly good genetic potential of the seeds, produced in '77 plot of the seed orchard of Japanese red pines at Anmyeon island, may be guaranteed. Observed results from the analysis of mating system of Japanese red pines in a '77 plot of the seed orchard may also provide useful information for the establishment and management of the seed orchard of the progressive generation.

• Korean Journal of Plant Resources
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• v.25 no.1
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• pp.63-71
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• 2012

To assess parameters of mating system in seed orchard, such as outcrossing rates, number of potential pollen contributors, and degree of pollen contamination, seeds, produced in '77 plot of the Japanese red pine (Pinus densiflora S et Z) seed orchard at Anmyeon island, were collected in 2007 and analysed by nSSR and cpSSR markers. Estimates of outcrossing rates ranged from 91.2 to 100% (mean 97.7%) on the basis of the analysis of cpSSR haplotypes and from 81.6 to 100% (mean 95.3%) on the basis of the analysis of nSSR genotypes. By cross checking of both DNA markers, seeds, presumed to be products of self pollination on the basis of single marker, were confirmed as outcrossed seeds, which resulted in cumulative outcrossing rates of 98.9%. On the basis of pooled cpSSR haplotype of each seed, the number of pollen contributors and paternal contribution rates were estimated as 14.8 and 0.512, respectively. In conclusion, considering pretty high level of outcrossing rates observed in a seed orchard, good genetic potential of the seeds, produced in '77 plot of the seed orchard of Japanese red pines at Anmyeon island, may be guaranteed. Investigated results from the analysis of mating system of Japanese red pines in a '77 plot of the seed orchard may also be expected to provide useful information for the management and establishment of the seed orchard of the progressive generation.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.367-370
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• 2005

The risk from genetically modified (GM) plants results from the possibility of gene contamination producing adverse effects on biological diversity by introducing herbicide or insect resistance into related plants or weeds (NAS 2002). The concern about the leakage of genes from GM plants into the environment has primarily focused on pollen that could be wind-borne for long distances. During the period of fisk assessment in Japan, physical containment is applied as a measure of reducing gene flow via the dispersal of pollen from GM plants into the surrounding environment In this study, we tried to estimate the effect of physically contained greenhouse covered by 1-mm fine mesh to reduce pollen dispersal by researching cross pollination rate between non-GM yellow maize in a greenhouse and silver maize outside the greenhouse.

• The Plant Pathology Journal
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• v.37 no.3
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• pp.299-306
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• 2021

Pollination is an essential process for plants to carry on their generation. Pollination is carried out in various ways depending on the type of plant species. Among them, pollination by insect pollinator accounts for the most common. However, these pollinators have be decreasing in population density due to environmental factors. Therefore, use of artificial pollination is increasing. However, there is a lack of information on microorganisms present in the artificial pollens. We showed the composition of bacteria structure present in the artificial pollens of apple, kiwifruit, peach and pear, and contamination of high-risk pathogens was investigated. Acidovorax spp., Pantoea spp., Erwinia spp., Pseudomonas spp., and Xanthomonas spp., which are classified as potential high-risk pathogens, have been identified in imported pollens. This study presented the pollen-associated bacterial community structure, and the results are expected to be foundation for strengthening biosecurity in orchard industry.

• Journal of Korean Society of Forest Science
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• v.104 no.4
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• pp.578-587
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• 2015

Nuclear SSR (nSSR) and chloroplast SSR (cpSSR) markers were analyzed to assess the parameters of mating system in seed orchard, such as outcrossing rates, the number of potential pollen contributors, paternal contribution rates, degree of pollen contamination, and biparental inbreeding ($t_m-t_s$). In 2006, 2007, seeds were collected from the seed orchard of Pinus densiflora, established in 1977 at Anmyeon island. Estimates of outcrossing rates ranged from 94.9 to 100% (mean 98.9%) in 2006 and from 91.2 to 100% (mean 97.7%) in 2007 on the basis of the analysis of cpSSR haplotypes and from 90.3 to 100% (mean 95.9%) in 2006 and from 81.6 to 100% (mean 95.3%) in 2007 on the basis of the analysis of nSSR genotypes. By cross checking of both DNA markers, mean cumulative outcrossing rates of 100% and 98.9% were estimated in each year. Mean contamination rates were estimated as 48.9% and 42.4%, respectively. On the basis of cpSSR haplotype observed in each seed, paternal contribution rates (the number of pollen contributors) were estimated as 0.458 (mean 16.2) in 2006 and 0.512 (mean 14.8) in 2007. In conclusion, considering pretty high level of outcrossing rates observed in a seed orchard, there might be little to be influenced by inbreeding depression for genetic potential of the seeds induced by selfing. Estimates of the mating system parameters obtained from the two reproductive years were not statistically different, which revealed stable genetic quality of seeds produced in different years. Observed results from this study may provide useful information for the management and establishment of the seed orchard of the progressive generation.

• Korean Journal of Breeding Science
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• v.40 no.3
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• pp.263-268
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• 2008

Estimates of genetic gain (in volume growth) and diversity (expressed as status number, $N_s$) were determined in a clonal seed orchard of Pinus koraiensis. The genetic thinning was based on clonal breeding values (represented by general combining ability) obtained from progeny tests, clonal fertility estimated by strobilus production, and clonal size variation determined by the ramet numbers per clone. Parental GCA values for volume growth were calculated, based on height and diameter at breast height measured from field trials. Clonal fertility was estimated from the assessments of strobilus production over twelve years from 1991 to 2003, and used for the calculation of status number. There are 179 clones and 5,268 ramets in 12ha area of P. koraiensis clonal seed orchard. Genetic gain and diversity estimates were determined under assumptions of 30% pollen contamination and inferior genetic value of contaminating pollen. Genetic gain increased as thinning rates were set from 10% to 60%. However, for the higher thinning intensities, the increase of genetic gain was not remarkable. Genetic thinning by means of truncation selection resulted in a greater genetic gain but a large decrease in status number. Status number was represented around 40 clones for 10% through 60% thinning intensities, but for the higher thinning intensities, it was a bit fluctuated. Based on the present results, it could be concluded that thinning rate should not be stronger than 60% to optimize genetic gain while conserving genetic diversity. Consequently 50% or 60% thinning rate might be appropriate for genetic thinning in the clonal seed orchard of P. koraiensis. The effect of pollen contamination on the genetic gain and the consequence of genetic thinning for seed production in the clonal seed orchard, and seed orchard management scheme were also discussed.

• Food Science and Industry
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• v.55 no.2
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• pp.166-175
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• 2022

Current food system has developed with the agricultural innovation to feed the increasing population of the world, but with high costs such as environmental contamination and inequality with low sustainability. Human has developed long history of mutualistic interaction with honey bee. This manuscript describes the multi-functionality of honey bee for food production. Firstly honey bee produces honey, bee pollen, royal jelly and propolis which are rich in functionality. Second honey bee serves as the main pollinator for crop production which is worth for 28% of total crop production values in Korea. Lastly honey bee can be an alternative meat produciton system with lower energy, carbon costs but higher nutritional security. This manuscript described those parts and discussed the multi-functionality of honey bees for eco-friendly food security pursuing lowered environmental cost and carbon-zero strategies in the climate change era.

• Journal of Korean Society of Forest Science
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• v.98 no.6
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• pp.696-702
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• 2009

Yellow poplar (Liriodendron tulipifera L.) is an insect-pollinated tree species with large, perfect flower, and its seed sets average only about 10 percent naturally. In its controlled pollination, pollination bags are usually taken to prevent unwanted pollination, but bagging is an expensive and time-consuming process. Therefore, this study was conducted to determine the need of pollination bag by estimating how much unintended pollination would occur when different cross methods were applied. Five different pollination methods were applied as follows: 1) natural open pollination (i.e. insect pollination) as a reference, 2) self-pollination; no removing reproductive organs with bagging, 3) open pollination; emasculated(removing sepal, petal and stamen) without bagging, 4) controlled pollination; emasculated with bagging and 5) controlled pollination; emasculated without bagging. Very low value of full seed rate (0.2%) was observed in method 3, it was suggested that removing stamen and petal restrict the activity of pollen vectors like bee. Difference in the full seed rate between method 4 and method 5 was not significant (27.9% versus 24.0%, respectively). Consequently, controlled pollination without bagging might be an alternative method for extensive breeding and mass production of seeds in yellow poplar.

• Journal of Korean Society of Forest Science
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• v.24 no.1
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• pp.53-61
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• 1974

Pine forests existing in Hong-do island were examined as regarding such characteristics, tree form, needle length, branch diameter, bark colors, ratio of summer wood to springwood, location and number of resin canal and other measurements. Hong-do island, located the furthest west part of Korea, suports only Pinus densiflora but very few Pinus thunbergii, in fact the present authors could not find any black pine individuals at all through investigation. Checking 14 individual trees of red pine, more than age of 20, of the genetic contamination by black pine, in a certain degree, exist. Here, a very dense foliage type and a very sparse foliage type tree of red pine growing nearby was examined and the unique fact was that each of those type has only one (average number 1.3) accessory resin canal, while other trees have three as minimum or more. The present authors thought this island had not been covered by any Pinus thunbergii but by Pinus densiflora. It was thought that Pinus thunbergii stands grown along the sea-coast of Korean Peninsula are pollen donors to this island.

• Korean Journal of Agricultural Science
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• v.41 no.3
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• pp.157-161
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• 2014

Understanding the gene flow from genetically modified (GM) crops to conventional crops is important to prevent and mitigate seed contamination caused by pollen-mediated gene flow. We conducted a field test to investigate the gene flow from diamondback moth resistant GM cabbage (Brassica oleracea var. capitata) containing cry1Ac1 gene, to a non-GM control line AD126. GM and non-GM cabbage plants were cultivated in the field and pollinated using Bombus terrestris under the nets during the flowering periods. After seeds were collected from non-GM plants, hybrids between them and the GM cabbages were screened by multiplex PCR targeting cry1Ac1 gene. Out of 878 germinated seedlings, 168 hybrids were found and the average gene flow frequency was 19.7%. Because cabbage is mainly pollinated by insect pollinators, large-scale field tests are needed to study gene flow of GM cabbage.