• Title/Summary/Keyword: pollen analysis

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Investigation of Fungal Strains Composition in Fruit Pollens for Artificial Pollination

  • Do, Heeil;Kim, Su-Hyeon;Cho, Gyeongjun;Kim, Da-Ran;Kwak, Youn-Sig
    • Mycobiology
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    • v.49 no.3
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    • pp.249-257
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    • 2021
  • Plants pollination are conducted through various pollinators such as wind, animals, and insects. Recently, the necessity for artificial pollination is drawing attention as the proportion of natural pollinators involved is decreasing over the years. Likewise, the trade in pollen for artificial pollination is also increasing worldwide. Through these imported pollens, many unknown microorganisms can flow from foreign countries. Among them, spores of various fungi present in the particles of pollen can be dispersed throughout the orchard. Therefore, in this study, the composition of fungal communities in imported pollen was revealed, and potential ecological characteristics of the fungi were investigated in four types of imported pollen. Top 10 operational taxonomic unit (OTU) of fungi were ranked among the following groups: Alternaria sp., Cladosporium sp., and Didymella glomerata which belong to many pathogenic species. Through FUNGuild analysis, the proportion of OTUs, which is assumed to be potentially plant pathogens, was higher than 50%, except for apple pollen in 2018. Based on this study of fungal structure, this information can suggest the direction of the pollen quarantine process and contribute to fungal biology in pollen

The Change of Vegetation Environment since middle-late Holocene in the lower reaches of Taehwa River, Taehwa-dong, Ulsan-si, Korea (울산 태화강 하류 태화동 홀로세 중·후기 식생환경 변화)

  • Kim, HyeRyung;Yoon, Soon-Ock;Hwang, Sangill
    • Journal of The Geomorphological Association of Korea
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    • v.23 no.2
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    • pp.1-13
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    • 2016
  • This study analyzed pollen compositions in archaeological site on alluvial plain of Taehwa River, Taehwa-dong, Ulsan-si vegetational and environmental change during 6,200~3,000 yr BP. The results consist upward of Pollen Zone I (Inner Bay environment), Pollen Zone II (Quercus-Alnus stage) and Pollen Zone III (Alnus stage). The pollen grains of Fagopyrum and reddish gray silty horizon including artifacts of the Bronze Age suggest that there was cultivation on dry-field around the study site, although it is not sure that there was paddy-field cultivation.

Gross Chemical Analysis for Honey and Pollen Load (봉밀(蜂蜜) 및 화분하(花粉荷)의 순도시험(純度試驗)과 성분조사(成分調査))

  • Suk, Kuy-Duk;Kim, Mi-Kyung
    • Korean Journal of Pharmacognosy
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    • v.14 no.4
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    • pp.197-200
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    • 1983
  • This study is concerned with quality and analysis of nutritive value of commercially available honey and pollen load. Of thirteen kind of commercially available honeys, acid levels were less than KP IV standard. Color reaction for ammonia, resorcine and varium chloride was negative and trace of starch and dextrine was not detected. There were no extraneous materials in honey samples. Specific gravity of sample was slightly higher than KP IV standard. Total ash lied between 0.01 and 0.15% of honey weight which was less than KP IV standard, except 0.56% of Castanea Honey. Studies on mineral compositions (AAS) for honeys and pollen loads showed that $Na^+,\;K^+,\;Ca^{2+},\;Fe^{2+}\;Cu^{2+}\;and\;CO^{2+}$ were the most commonly occuring minerals. Pollen loads showed higher levels of mineral contents than honeys. Castanea Honey revealed rich in minerals. $Cd^{2+}\;and\;Pb^{2+}$ were found relatively higher level in Robinia Honey. Reducing sugar level showed $60{\sim}70$ in honeys and $25{\sim}30$ in pollen loads. Non reducing sugar varied between 2 to 7% in pollen loads.

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The Environmental Change and Geomorphic Development of Unsan Alluvial Plain in Kangreung City during the Late Holocene (강릉 운산충적평야의 홀로세 후기의 환경변화와 지형발달)

  • 윤순옥
    • Journal of the Korean Geographical Society
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    • v.33 no.2
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    • pp.127-142
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    • 1998
  • Unsan alluvial plain is the backmarsh of Seomseokcheon which is a river originated from Chilseongdae(954m) on Mts Taeback, flowing into Donghae in southern Kangreung City, Kangwon Province. The vegetation change, geomorphic develoopment and depositional environment during the late Holocene have been investigated, using the methods such as boring, pollen analysis and radiocarbon dating. Because the deposits fo the study area are mainly consisted of peat and paety sand, they contained many pollen fossils. The peat layer has been sedimented since the high sea-level periods, 3,200 y. BP, and the records of vegetation change until now has well preserved here. According to archeological researches and the results of pollen analysis in east coast of Korea, it is supposed that the prehistoric rice farming in this area has begun since ca. 1,800 y. BP.

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Vegetational History of the Mulyeongari Fen by Pollen Analysis in Jeju Island, Korea (제주도 물영아리늪 퇴적물의 화분분석에 의한 식생변천)

  • Lee, Chang-Su;Kang, Sang-Joon;Choi, Kee-Ryong
    • Journal of Environmental Science International
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    • v.20 no.3
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    • pp.341-350
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    • 2011
  • For the reconstruction of the past vegetational changes in Jeju Island, Korea, pollen analysis and radiocarbon dating on the sediments obtained from the Mulyeongari fen were carried out. By the results, the vegetational changes around the Mulyeongari fen from ca. 3,300 cal. yr BP to the present can be interpreted and reconstructed. The pollen record from the Mulyeongari fen was divided into two local pollen assemblage zones and three sub-pollen assemblage zones. Zone I (Quercus-Carpinus-Herbs Zone) was characterized by the predominance of Quercus(30~63%), Carpinus(9~35%) and herbs(40~424%). Zone II was characterized by three sub-pollen assemblage zones and the high occurrence ratio of the tree layer in comparison with Zone I. In Zone IIa (Quercus-Carpinus Zone), herbs(3~161%) were drastically decreased in predominance of Quercus(28~56%) and Carpinus(14~31%). In Zone IIb (Carpinus-Quercus Zone), Carpinus(36~48%) was preferentially increased but Quercus(29~39%) was slightly decreased. In Zone IIc (Quercus-Carpinus Zone), Carpinus(26~38%) was decreased inversely but Quercus(36~50%) was increased. In addition, Cyperaceae was also increased to 52%. Consequently, it is suggested that cool temperate southern/sub-montane vegetation composed of Quercus and Carpinus which was physiognomy of deciduous broad leaved forest was distributed around the Mulyeongari fen from ca. 3,300 cal. yr BP. In addition, Cyclobalanopsis(4~23%), Castanopsis(1~12%) and Myrica(under 1%) which are warm-temperate evergreen deciduous forest components were constantly appeared from this period. Accordingly, it can be inferred that the present vegetation type around the Mulyeongari fen was formed from ca. 180 cal. yr BP.

The Holocene Environmental Change and Reconstruction of the Palaeogeography at Ilsan Area with the Special Reference to Pollen Analysis (花粉分析을 중심으로 본 一山지역의 홀로세 環境變化와 古地理復元)

  • Yoon, Soon-Ock
    • Journal of the Korean Geographical Society
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    • v.32 no.1
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    • pp.15-30
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    • 1997
  • This paper concerns the Holocene environmental change with vegetational history and sea-level fluctuation at Ilsan area by the analytical data of pollen, sedimentary facies and $C^14$-dating. The hypothetic palaeogeographic maps of the vegetation cover have been reconstructed with the reference to the periods of pollen zone. The environmental characteristics from the pollen zonation have been summerized as follows. 1)Pollenzone I(3.75~5.75m) showed the period of Alnus-and EMW-dominance. The study area was very humid under the influence of the transgression spreading widely from the rapid sea-level rise during the period(8,000~4,200y.BP). 2)Pollen zone II(5.75~6.35m) has been influenced by the fall of the sea-level and ground water surface. This zone(4,200~2,300y.BP) represented the period of spore~ and NAP-dominance with the increase of Pinus. 3) Pollen zone III(6.35~6.55m) has reflected the influence of the transgression and human interferences together. This zone(2,300~1,800y.BP) represented the period of NAP-dominance. The boundary between Subzone Ilb and Pollen zone III represents the same characteristics as what Weber says Grenzhorizont.

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An Increment of Crossing Efficiency with Consideration of Pollen Viability Analysis in Rose (장미 교배 효율 증대를 위한 화분 임성 검정)

  • Hwang, Yoon Jung;Song, Chang Min;Kwon, Min Kyung;Kim, Sung Tae;Kim, Won Hee;Han, Youn Yol;Han, Tae Ho;Lim, Ki Byung
    • FLOWER RESEARCH JOURNAL
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    • v.18 no.3
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    • pp.193-200
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    • 2010
  • Current rose cultivars are all composed of heterozygous genome due to long history of out crossing including interspecific hybridization. It has been adapted by artificial selection and crossing by breeders that mainly based on the crossing with fertile pollen derived from inter- or intra-specific hybridization. Pollen viability and germination ability tests provide valuable information for the designing of parentage for more successful breeding efficacy. In this study, we tested the pollen viability and germination ability in seven rose cultivars to find any relationship among several factors including pollen size, ploidy levels, and crossing compatibility. The pollen viability showed wide ranges from 39% 'Pinocchio' as minimum to 82% 'Scarlet Mimi' as maximum, whereas pollen germination rate were from 1% 'Mini Rosa' to 41% 'Scarlet Mimi' as a highest. Pollen size ranged from 41.3 to $45.4{\mu}m$ in large sized pollen and 30.7 to $37.4{\mu}m$ in small sized pollen. The mean diameter of large sized pollen is approximately 10-40% bigger than that of small sized pollen. There are positive relationships among ploidy level, total chromosome length, and pollen size. Crossing list showed that seed setting ratio and seed germination were related to pollen viability, pollen germination, and ploidy level.

Influence of gene flow from GM to non-GM soybeans by the size of the pollen donor

  • Lee, Bumkyu;Oh, Sung-Dug;Chang, Ancheol
    • Korean Journal of Agricultural Science
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    • v.45 no.4
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    • pp.591-600
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    • 2018
  • The use of genetically modified (GM) crops has increased continuously over the world, and concerns about the potential risks of GM crops have also risen. Although, until now, GM crops have not been cultivated commercially in Korea, it is necessary to develop technology for the safe evaluation of GM crops. In this study, we investigated the influence of gene flow from GM to non-GM soybeans by the size of the pollen donor. In the experimental design, GM soybeans were placed in the center as a pollen donor and non-GM soybeans were placed in four directions as the pollen receivers. Three sizes of pollen donor were designed as $90cm{\times}90cm$, $180cm{\times}180cm$, and $360cm{\times}360cm$. A total 22,719 seeds were collected from non-GM soybeans, and 14 hybrids were finally obtained through herbicide resistance screening and PCR analysis. The highest hybridization rate was 0.78% at a distance of 15 cm from a $360cm{\times}360cm$ GM pollen donor, and the farthest distance of hybridization was 180 cm from a GM pollen donor which was $360cm{\times}360cm$ in size. Ten hybrids were found among the 14 hybrids at the $360cm{\times}360cm$ pollen donor size, 3 hybrids at $180cm{\times}180cm$, 1 hybrid at $90cm{\times}90cm$. From these results, it could be concluded that with the larger pollen donor size, more hybridization occurred in soybeans.

Morphological Features of Pollen Grains in Portulaca

  • Kim, InSun
    • Applied Microscopy
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    • v.43 no.2
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    • pp.75-80
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    • 2013
  • The morphological features of pollen grains collected from the broad-leaved and cylindrical-leaved Portulaca species of the Hawaiian Islands were investigated. The variation in size and surface pattern were examined using scanning electron microscopy and statistical analysis. Pollens of the Portulaca were apolar grains of monads exhibiting apertures, colpi, and relatively thin echini. Of particular interest was the differing size of pollen grains among the species studied. Pollens of the broad-leaved Portulaca were considerable in size with a length of about $73{\sim}86{\mu}m$, while the cylindrical-leaved Portulaca tended to have smaller pollen grains ranging from 50~65 in diameter. The smallest pollens were found in cultivar 2 having an average of $43{\mu}m$. The pollen was intectate, and the exine surface appeared granulous, having sculptured elements of spinules and puncta. Two types of puncta were discerned; one forming an operculum, and the other, a simple perforation. Numerous spinules and small puncta were found throughout the cylindrical-leaved Portulaca. In the present study, morphological features of pollen grains from ten Hawaiian Portulaca species, including endemic and unknown taxa, revealed the aforementioned pattern of variation. This paper aim to provide morphological information that could be of phylogenetic value within the Hawaiian Portulaca.

Effects of Cattail Pollen Powders on the Rheology of Dough and Processing Adaptability of White Pan Bread (부들화분을 첨가한 밀가루 반죽의 물성과 제빵 적성)

  • Lee, Bung-Chan;Joung, Yong-Myeon;Hwang, Seong-Yun;Lee, Jong-Hwa;Oh, Man-Jin
    • Food Science and Preservation
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    • v.16 no.4
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    • pp.525-533
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    • 2009
  • To explore cattail pollen powder as a functional food ingredient, we analyzed the general components of pollen powder, tested changes in the physical properties of dough containing the powder, and investigated the process ability of powder-containing dough in bread making by adding 3%, 6%, or 9% by weight of pollen powder to wheat flour. Cattail pollen powder consisted of (all w/w) 12.7-13.2% water, 15.7-17.8% crude protein, 1.3% crude fat, 7.5-7.7% free sugar, 14.7-18.6% crude fiber, 3.4-4.9% pollen, and 49.7-55.9% soluble nitrogen-free extract (NFE). Analysis of the physical properties of dough mixed with pollen powder showed that as more pollen powder was added, the absorption rate increased, but dough stability decreased. With increasing levels of cattail pollen powder, the falling number decreased, and amylase activity increased. Fermentability was highest in dough made with 3% by weight of pollen powder, and the bread product made from such dough had the greatest volume. As more cattail pollen powder was added, the moisture activity in dough tended to decrease to a greater extent than seen in control dough, and this tendency increased with time. We found that longer storage periods were associated with greater hardness and springiness, which indicated degradation in product quality. Therefore, it is suggested that bread products containing cattail pollen powder should be consumed within 3 days of preparation. In a taste survey, bread baked with 3% (w/w) cattail pollen powder scored highest in all questionnaire items.