• 한국미생물·생명공학회지
• /
• 제33권2호
• /
• pp.84-89
• /
• 2005

Phosphomannomutase는 진핵 생물과 원핵 생물에 있어서 중요한 효소로, ${\alpha}$-D-mannose 6-phosphate를 ${\alpha}$-D-mannose 1-phosphate로 전환시켜 GDP-mannose를 생산한다. 이 기질은 여러 대사 경로에서 중요하게 작용하는 mannosyl기를 제공하도록 돕는다. 본 논문에서는 Sphingomonas chungbukensis DJ77에서 phosphomannomutase를 암호화하는 유전자를 유전체 library로부터 동정하고 이를 pmmC로 명명하였으며, 이를 발현 vector에 클로닝하고 염기서열을 분석하였다. 유전자 pmmC는 ATG를 개시 코돈으로 사용하고, TAG를 종결 코돈으로 사용하는 750 bp의 open reading frame임을 확인하였고, 이 ORF의 5 bp앞쪽으로 리보좀 결합 부위가 존재한다. 이 ORF로부터 유추되는 아미노산은 249개이며, 단백질 분자량은 약 27.4 kDa이다. 이 유전자를 구성하는 아미노산 서열은 NCBI의 conserved domain search를 통한 분석으로 eukaryotic phosphomannomutase와 약 $86.9\%$ 유사성이 있음을 나타냈고, 기질에 대한 활성을 측정한 결과 pmmC 유전자가 암호화하는 단백질이 phosphomannomutase임을 확인할 수 있었다.

• 한국건축시공학회:학술대회논문집
• /
• 한국건축시공학회 2022년도 가을 학술논문 발표대회
• /
• pp.71-72
• /
• 2022

In this paper, the performance evaluation of the vibration damping ratio according to the polymer mixing ratio of the polymer modified mortar used as the floor finishing material of the apartment building structure was evaluated. To compare the vibration damping rate, ordinary potland cement (OPC) mortar and polymer modified mortar (PMM) were prepared. In addition, the mixed polymer was mixed with Styrene Butadiene Rubber (SBR) liquid polymer with a solid content of about 49%. Accordingly, the W/C of the test specimen was adjusted and compounded, and the experiment was conducted by mixing 5 types of the test specimen: OPC-60, PMM-5%, PMM-10%, PMM-15%, and PMM-20%. In addition, in order to adjust the W/C of the specimen, the fluidity of each specimen was set as 210 (±5) mm. The specimens measured density and flow in fresh mortar and after curing for 28 days, flexural strength, compressive strength and ultrasonic pulse were measured. The attenuation rate was shown. The experimental results showed that the density increased according to the mixing of the polymer, the flexural strength increased as the mixing rate of the polymer increased, and the compressive strength was decreased. In addition, it was shown that the vibration damping rate increases with the increase in the amount of polymer incorporated.

• 대한조선학회지
• /
• 제27권3호
• /
• pp.31-37
• /
• 1990

선박의 방향안정성 등을 논할 때 필요하게 되는 선형조종미계수에 대해서는 이론적, 실험적으로 그 추정법이 제안되고 있다. 이중, 실험적 방법은 모형선에 규칙적인 강제동요를 시켜서 원하는 값들을 구하고 있으나, 한규칙 동요에 대해서 그 주파수에 대한 값만 나오므로 넓은 범위의 운동주파수에 걸쳐 미계수의 값들을 얻고자 할 때는 방대한 회수의 실험을 행해야 한다, 이러한 단점을 개선하기 위해 고안된 것이 본 논문에서 취급하는 Transient Maneuvering Method(TMM)로서, 기본원리는 모형에게 과도적 운동을 시켜 그로 인한 동유체력을 계측하고 이를 Fourier 변환하므로서 여러가지 주파수에 대한 미계수의 값을 얻게 되는 것이다. 종래의 이 분야 연구자들은 모형을 움직일 때 전동모터를 사용했으나 모터를 사용할 경우 원하는 과도 운동을 충분히 시킬 수 없으므로 본 논문에서는 유압 펌프를 사용하여 좋은 효과를 얻었다. 그 외에도 입력신호를 다소 개량하여 최종결과의 S/N비를 개선하는데 성공하였다. 실험에 사용한 모델은 Todd의 계열 60(Cb=0.7) 2M급이고, 이 결과를 종래의 PMM 시험결과와 비교하였다.

• 여성건강간호학회지
• /
• 제10권2호
• /
• pp.162-170
• /
• 2004

Purpose: The purpose of this study was to differentiate between women with three perimenstrual symptom severity patterns : premenstrual syndrome(PMS), premenstrual magnification(PMM), and low symptom(LS), and to explore the related dietary factors to premenstrual symptoms. Method: Women were asked to keep a diary record of perimenstrual symptoms and food intake for 50 days. Result: Symptom patterns were defined for 26 among 38 women ; Eight(21.1%) demonstrated a PMS pattern, three(7.9%) demonstrated a PMM pattern, and fifteen(39.5%) exhibited a LS pattern. There were significant differences in symptom scores during the premenstrual phase($x^2=19.30$, p=.000), menstrual phase($x^2=13.32$, p=.001), and post menstrual phase($x^2=9.93$, p=.007) for three groups. Protein, vit E, vit C, niacin, folic acid, and phosphorus in the premenstrual phase, and energy, and vit B6 in the menstrual period were significantly different between the three groups. Among dietary compositions, amino acids, lipids, fatty acids, saturated fatty acids, natrium, vit B6, niacin, and vit E were negatively related to PMS symptoms. Conclusion: Pattern of perimenstrual symptoms should be differentiated for individualized PMS management. As a more efficient diet assessment for PMS women, randomized nutritional analysis during the 3 phases of the menstrual cycle should be done and a replication study is necessary with a larger sample.

• 한국지하수토양환경학회지:지하수토양환경
• /
• 제27권6호
• /
• pp.47-57
• /
• 2022

A number of different methods have been used for modeling the sorption of volatile organic chlorinated compounds such as tetrachloroethylene/perchloroethylene (PCE). In this study, PCE was adsorbed in several natural sorbents, i.e., Pahokee peat, vermicompost, BionSoil^®, and natural soil, in the batch experiments. Several sorption models such as linear, Freundlich, solubility-normalized Freundlich model, and Polanyi-Manes model (PMM) were used to analyze sorption isotherms. The relationship between sorption model parameters, organic carbon content (f_oc), and elemental C/N ratio was studied. The organic carbon normalized partition coefficient values (log K_oc = 1.50-3.13) in four different sorbents were less than the logarithm of the octanol-water partition coefficient (log K_ow = 3.40) of PCE due to high organic carbon contents. The log K_oc decreased linearly with log f_oc and log C/N ratio, but increased linearly with log O/C, log H/C, and log (N+O)/C ratio. Both log K_F,oc or log K_F,oc decreased linearly with log f_oc (R² = 0.88-0.92) and log C/N ratio (R² = 0.57-0.76), but increased linearly with log (N+O)/C (R² = 0.93-0.95). The log q_max,oc decreased linearly as log f_oc and log C/N increased, whereas it increased with log O/C, log H/C and log (N+O)/C ratios. The log q_max,oc increased linearly with (N+O)/C indicating a strong dependence of q_max,oc on the polarity index. The results showed that PCE sorption behaviors were strongly correlated with the physicochemical properties of soil organic matter (SOM).

• 미생물학회지
• /
• 제41권3호
• /
• pp.168-176
• /
• 2005

갯벌 퇴적물에 존재하는 병원성 해양미생물인 Vibrio vulnificus를 신속하고 정확하게 검출하기 위해 PCR, Southern hybridization 방법과 real-time PCR을 수행하여 검출 민감도를 비교하였다. 갯벌 퇴적물로부터 bead beater를 이용한 물리적 방법으로 DNA 조추출액을 얻고 상용화된 키트 (Geneclean turbo Kit)를 이용하여 부식물질(humic substances)을 제거하였다. 병원성에 관련된 3 종의 유전자(hemolysin, vvhA; phosphomannomutase, pmm; metalloprotease, vvpE)를 대상으로 설계한 프라이머 셋을 동시에 사용하는 multiplex PCR 방법과 Southern hybridization과 병행한 방법(PCR/Southern hybridization)을 수행하였다. Real-time PCR은 hemolysin 유전자(vvhA)에 특이한 프라이머와 TaqMan 탐침을 사용하였다. 전처리하지 않은 갯벌 퇴적물의 경우, PCR/Sourthern hybridization과 real-time PCR 방법의 검출 민감도는 퇴적물 1 g 당 약 $10^2$ 개의 세포 수준이었다. 농후처리액(APW; alkaline peptone water)으로 $35^{\circ}C$에서 $2{\~}3$시간, 8시간 중균 배양할 경우 갯벌 퇴적물 1 g 당 $2{\~}10$개 세포가 존재할 때 PCR/Southern hybridization 방법과 real-time PCR 방법으로 각각 검출할 수 있었다. 전처리 과정을 포함하여 real-time PCR은 $6{\~}7$시간, PCR/Sourthern hybridization은 약 36시간이 소요되었다.

• 한국지하수토양환경학회지:지하수토양환경
• /
• 제27권6호
• /
• pp.11-21
• /
• 2022

Sorption of phenanthrene (PHE) and pyrene (PYR) in several sorbents, i.e., natural soil, BionSoil^®, Pahokee peat, vermicompost and Devonian Ohio Shale and a surfactant (hexadecyltrimethyl ammonium chloride)-modified montmorillonite (HDTMA-M) were investigated. Pyrene exhibited higher sorption tendency than phenanthrene, as predicted by its higher octanol to water partition coefficient (K_ow). Several sorption models: linear, Freundlich, solubility-normalized Freundlich model, and Polanyi-Manes model (PMM) were used to analyze sorption isotherms. Linear isotherms were observed for natural soil, BionSoil^®, Pahokee peat, vermicompost, while nonlinear Freundlich isotherms fitted for Ohio shale and HDTMA-M. The relationship between sorption model parameters, organic carbon content (f_oc), and elemental C/N ratio was studied. In the binary competitive sorption of phenanthrene and pyrene in natural soil, competition between the solutes caused reduction in the sorption of each solute compared with that in the single-solute system. The ideal adsorbed solution theory (IAST) coupled with the single-solute Freundlich model was not successful in describing the binary competitive sorption equilibria. This was due to the inherent nature of linear sorption of phenanthrene and pyrene in natural soil. The result indicates that the applicability of IAST for the prediction of binary competitive sorption is limited when the sorption isotherms are inherently linear.