• Title/Summary/Keyword: plcR

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Classification and Characterization of Bacteriophages of Lectobacillus casei (Lactobacillus casei Bacperiophage의 분류 및 특성에 관한 연구)

  • 김영창;박민철;강국희;윤영호;이광웅
    • Korean Journal of Microbiology
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    • v.17 no.4
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    • pp.165-178
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    • 1979
  • Phages of Lactobaciilus casei (PLC) isolated from plant drainage were classified and characterized. The results are as follows : 1. On the basis of host range pattern, phages could be divided into 2 groups (PLC-B and PLC-C). PLC-B group phages could be further divided into 5 sub-groups $(B_1, \;B_2, \;B_3, \;B_4, \;and\;B_5)$. Although PLC-C group phages had the same host range, they could be also divided into 2 sub-groups $(C_1\;and\;C_2)$ by morphlogical type. 2. It was $B_3$ group phages that represented a major proportion (44.4%) of phages tested. However, $B_1$ group phages were shown to have the widest host range. 3. Electron micrographs revealed that the phages fell into three different morphological types. $(B_1, \;B_2, \;and\;B_3)$ group phages hd a hexagonal head (52nm in diameter) and a sheathless noncontractile (245 nm in length). $B_4\;and\;C_2$ group phages had a hexagonal head (56 nm) and a short flexible tail (169nm) having no sheath. $B_5\;and\;C_1$ group phages were shown to have a hexagonal head (81 nm) and a contractile tail (140 nm) having a sheath, a base plate and tail fibers. 4. The inactivation of the phages by antisera indicated that serological relationships correlated completely with morphological types. 5. $B_1, \;C_1\;and\;C_2$ group phages produced a large (1, 2 mm in diameter) plaque with a clear ring. The morphology of plaques of $B_3\;and\;B_5$ group phages was the same as those produced by the above, but the average plaque sizes for $B_3\;and\;B_5$ were 0.8 mm abd 0.5 mm, respectively. $B_2\;and\;B_4$ group phages produced a small (0.5 mm) turbid plaque with an irregular edge. 6. The latent period and the average burst size of $B_1\;and\;B_3$ group phages were 90 min and 100, respectively. These phages reuqired calcium ions for their miltiplication. 7. $B_3$ group phages could not be absrobed to R-variant $KC_1$. 8. The order of resistance of phages to heat was $B_2\;>\;B_1, B_4\;and\;B_5\;>\;B_3\;and\;C_2, \;B_5$ group phages were more stable than $B_3$ in various pH values. $C_2$ group phages were more sensitive to UV irradiation than $B_1\;and\;B_3$ group phages. 9. Strains YIT9018 and IAM 1043 were induced by mitomycin C treatment. Phage particles detected in the lysates had a hexagonal head (38 and 49 nm, respectively), but no tail. Any sensitive indicator strain could not be isolated in spite of repaeated trials.

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The Development of a Multi-Purpose Irradiator and the Characteristic of Dose Distribution (다목적 방사선 조사장치 개발 및 선량분포특성)

  • Lee, Dong-Hoon;Ji, Young-Hoon;Lee, Dong-Han;Kim, Yoon-Jong;Hong, Seung-Hong
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.39 no.6
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    • pp.42-48
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    • 2002
  • The design, construction and performance test of a convenient multi-purpose irradiator is described. A multi-purpose irradiator using Cesium-137 has been developed for studies of low dose radiation effects in biology and for calibration of Thermo Luminescent dosimeter(TLD). During the operation, three rods of radioactive material which are 10cm in length revolve 180 degrees and irradiate biological samples, or TLD, and return to their shielded position, after the programmed time. A programmable Logic Controller(PLC) controls the sequence of operation, interlock, motor rotation and safety system. The rotation speed of biological samples can vary up to 20 RPM. A real time monitoring system was also incorporated to check and control the operation status of the irradiator. The capacity of the irradiation chamber was 4.5 liters. The isodose distribution at arbitrary vertical planes was measured by using film dosimetry. The dose-rate was 0.13 cGy/min in air and 0.11 cGy/min in water equivalent material in the case of Cesium-137. Range of activity was 2 Ci. The homogeneity of dose distribution in the chamber was ${\pm}$7%. The actual radiation level on the surface was within permissible levels. The irradiator had a maximum 0.35 mR/min radiation leakage on its surface.

Wind Generation Monitoring System Based on IEC61400-25 International Standard (IEC61400-25 국제 표준 프로토콜을 적용한 풍력 발전 모니터링 시스템 개발 사례)

  • Lee, Duck-Su;Lee, Jun-Chul;Hong, Jung-Gi
    • Proceedings of the KIEE Conference
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    • 2008.07a
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    • pp.227-228
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    • 2008
  • This paper presents monitoring system based on IEC61400-25 for 750kW-class DFIG wind power generation system. It is consists of wind turbine PLC, Local and Remote I/O Server, human machine interface, and Web-server. Proposed System has been demonstrated in Daegi-ri, Kangwon-do, which aims to test IEC61400-25 communication capability of monitoring system and evaluate the performance of 750kW-class WTS.

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Design of an Inductive Coupler for Power Transmission line (송전선용 대용량 신호결합장치의 설계)

  • Kim, H.S.;Byun, W.B.;Kim, J.R.;Bae, E.R.;Lee, D.C.;Lee, H.Y.;Lee, J.H.;Ji, M.K.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.11a
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    • pp.439-440
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    • 2008
  • An inductive coupler, which feeds communication to the electric power transmission line, is required to establish Power Line Communication(PLC). The electro-magnetic property of magnetic core and design technology for coupler are very important to manufacture an inductive coupler for power transmission line. The magnetic core with superior electro-magnetic property was manufactured by using nano-crystalline alloy and an inductive coupler, which can operate at the maximum 2,000 A current, was designed and manufactured by establishment of current saturation, signal out winding, and electro-magnetic simulation in this study. Communication speed of 14 Mbps in 600 m communication distance of the real electric power transmission line was obtained by using the inductive coupler and application possibility of the inductive coupler for the electric power transmission line was certified.

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Development of Reefer Container Real-time Management System (실시간 냉동컨테이너 관리 시스템 개발)

  • Choi, Sung-Pill;Jung, Jun-Woo;Moon, Young-Sik;Kim, Tae-Hoon;Lee, Byung-Ha;Kim, Jae-Joong;Choi, Hyung-Lim;Lee, Eun-Kyu
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.19 no.12
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    • pp.2917-2923
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    • 2015
  • In spite of a recent trend of container ships becoming larger in size, the current circumstance is that managing reefer containers marine transportation is being mostly dependent upon manpower. Particularly, in the case of bad weather or nighttime, reefer containers are not being monitored due to lack of safety device. For the purpose of reducing such risk, IMO is recommending a system using PLC but the system is not being used. In addition, they are still relying on manpower for control and reliability of freight in transit is decreasing due to lack of information during marine transportation for every subject related to freight as well as shipper. Accordingly, the purpose of this paper is to propose a real-time reefer container management system to effectively control all reefer containers widely being used across the world.

A Study on the Optical Element Alignment of Ultra Precision Multi-Axis Stage (극초정밀 다축 위치제어장치의 광소자 정렬 특성에 관한 연구)

  • Jeong S.H.;Cha K.R.;Kim H.U.;Choi S.B.;Kim G.H.;Park J.H.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2005.06a
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    • pp.1190-1193
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    • 2005
  • In recent years, as the demands of VBNS and VDSL increase, the development of kernel parts of optical communication such as PLC(Planar Light Circuit), Coupler, and WDM elements increases. The alignment and the attachment technology are very important in the fabrication of optical elements. In this paper, the optical alignment characteristics of multi-axis ultra precision stage were studied. The alignment algorithms were studied for applying to the ultra precision multi-axis stage. The alignment algorithm is comprised of field search and peak search algorithms. The contour of optical power signals can be obtained by field search and the precise coordinate can be found out by peak search. Two kinds of alignments, such as 1 ch. input vs. 1 ch. output optical stack, and 1 ch. input vs. 8 ch. output PLC stacks were performed for investigating the alignment characteristics.

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Signal Transmission Properties of the Inductive Coupler for PLC (전력선통신용 비접촉식 커플러의 신호전송 특성 향상 연구)

  • Kim, Hyun-Sik;Kim, Jong-Ryung;Huh, Jeong-Sub;Lee, Hae-Yeon;Lee, Jun-Hui;Jung, Won-Chae;Oh, Young-Woo;Byun, Woo-Bong
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2006.11a
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    • pp.239-240
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    • 2006
  • 저손실 고투자율 특성을 가지는 ${\alpha}-Fe$ 나노결정 자심재료를 제조하기 위한 열처리 온도 변화에서, $510^{\circ}C$의 열처리 조건에서 가장 높은 투자율과 신호전송 특성을 나타내었다. 저주파 대역에서의 신호 전송 특성은 자심재료의 자기적 특성에 지배적인 영항을 받으며, 고주파 대역의 신호전송 특성은 임피던스 매칭으로 향상시킬 수 있었다. 그리고 커플러의 출력부에 신호증폭장치를 삽입하여 자기유도에 의해 신호가 커플링될 때 발생하는 삽입손실을 보완하여 커플러의 특성을 향상시킬 수 있었다.

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CD40 Co-stimulation Inhibits Sustained BCR-induced $Ca^{2+}$ Signaling in Response to Long-term Antigenic Stimulation of Immature B Cells

  • Nguyen, Yen Hoang;Lee, Ki-Young;Kim, Tae-Jin;Kim, Sung-Joon;Kang, Tong-Mook
    • The Korean Journal of Physiology and Pharmacology
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    • v.15 no.3
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    • pp.179-187
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    • 2011
  • Regulation of B cell receptor (BCR)-induced $Ca^{2+}$ signaling by CD40 co-stimulation was compared in long-term BCR-stimulated immature (WEHI-231) and mature (Bal-17) B cells. In response to long-term pre-stimulation of immature WEHI-231 cells to ${\alpha}$-IgM antibody (0.5~48 hr), the initial transient decrease in BCR-induced $[Ca^{2+}]_i$ was followed by spontaneous recovery to control level within 24 hr. The recovery of $Ca^{2+}$ signaling in WEHI-231 cells was not due to restoration of internalized receptor but instead to an increase in the levels of $PLC{\gamma}2$ and $IP_3R-3$. CD40 co-stimulation of WEHI-231 cells prevented BCR-induced cell cycle arrest and apoptosis, and it strongly inhibited the recovery of BCR-induced $Ca^{2+}$ signaling. CD40 co-stimulation also enhanced BCR internalization and reduced expression of $PLC{\gamma}2$ and $IP_3R-3$. Pre-treatment of WEHI-231 cells with the antioxidant N-acetyl-L-cysteine (NAC) strongly inhibited CD40-mediated prevention of the recovery of $Ca^{2+}$ signaling. In contrast to immature WEHI-231 cells, identical long-term ${\alpha}$-IgM pre-stimulation of mature Bal-17 cells abolished the increase in BCR-induced $[Ca^{2+}]_i$, regardless of CD40 co-stimulation. These results suggest that CD40-mediated signaling prevents antigen-induced cell cycle arrest and apoptosis of immature B cells through inhibition of sustained BCR-induced $Ca^{2+}$ signaling.

Application of the Laser Vision Sensor for Corrugated Type Workpiece

  • Lee, Ji-Hyoung;Kim, Jae-Gwon;Kim, Jeom-Gu;Park, In-Wan;Kim, Hyung-Shik
    • 제어로봇시스템학회:학술대회논문집
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    • 2004.08a
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    • pp.499-503
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    • 2004
  • This application-oriented paper describes an automated welding carriage system to weld a thin corrugated workpiece with welding seam tracking function. Hyundai Heavy Industries Corporation has developed an automatic welding carriage system, which utilizes pulsed plasma arc welding process for corrugated sheets. It can obtain high speed welding more than 2 times faster than traditional TIG based welding system. The aim of this development is to increase the productivity by using automatic plasma welding carriage systems, to track weld seam line using vision sensor automatically, and finally to provide a convenience to operator in order to carry out welding. In this paper a robust image processing and a distance based tracking algorithms are introduced for corrugated workpiece welding. The automatic welding carriage system is controlled by the programmable logic controller(PLC), and the automatic welding seam tracking system is controlled by the industrial personal computer(IPC) equipped with embedded OS. The system was tested at actual workpiece to show the feasibility and performance of proposed algorithm and to confirm the reliability of developed controller.

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The Development of Electric Vehicle Slow Charging System Applying ISO/IEC 15118 Power Line Communication (ISO/IEC 15118 국제표준기반 충전기용 전력선 통신을 적용한 완속 충전기 개발)

  • Oh, JeongHun;Baek, Yohan;Oh, SungMin;Choi, YoungGon;Seo, JeongDueok
    • Proceedings of the KIPE Conference
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    • 2013.11a
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    • pp.83-84
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    • 2013
  • 최근 국내에서 진행중인 스마트 그리드(Smart Grid) 사업 분야에 속해 있는 스마트 운송(Smart Transportation) 사업의 하나로 전기차 운영이 핵심이 되어 진행이 되고 있다. 그러나 실제적으로 전기차를 원활히 운영하기 위해서는 극복해야 할 여러 문제가 있으며 대표적으로 주행 거리를 좌우하는 배터리 용량 문제와 고객이 어디서든 전기차를 사용하고 충전하는데 불편이 없을 만큼 충분한 충전 인프라 구축 문제가 있다. 충전 인프라는 현재, 완속 충전기와 급속 충전기로 구성되어 있으나 본 논문에서는 완속 충전기에 한정하여 서술하였고 인프라 구축에 있어 표준화 일환으로 미국자동차기술학회에서 제시하는 SAE-J1772 규격에 맞추어 개발한 자사의 완속 충전기에 2012년 독일과 미국의 자동차 업체 주도로 발표된 국제표준(ISO/IEC 15118, Vehicle-to-Grid Communication Interface) 기술 규격이며 향후 충전 인프라에 적용 예상이 되는 PLC 통신 방식을 적용한 완속 충전기 개발에 관하여 소개하도록 하였다.

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